557 resultados para tir-fccs


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The DAN/TIR mannoprotein genes of Saccharomyces cerevisiae (DAN1, DAN2, DAN3, DAN4, TIR1, TIR2, TIR3 and TIR4) are expressed in anaerobic cells while the predominant cell wall proteins Cwp1 and Cwp2 are down-regulated. Elements involved in activation and repression of the DAN/TIR genes were defined in this study, using the DAN1 promoter as a model. Nested deletions in a DAN1/lacZ reporter pinpointed regions carrying activation and repression elements. Inspection revealed two consensus sequences subsequently shown to be independent anaerobic response elements (AR1, consensus TCGTTYAG; AR2, consensus AAAAATTGTTGA). AR1 is found in all of the DAN/TIR promoters; AR2 is found in DAN1, DAN2 and DAN3. A 120 bp segment carrying two copies of AR1 preferentially activated transcription of lacZ under anaerobic conditions. A fusion of three synthetic copies of AR1 to MEL1 was also expressed anaerobically. Mutations in either AR1 site within the 120 bp segment caused a drastic loss of expression, indicating that both are necessary for activation and implying cooperativity between adjacent transcriptional activation complexes. A single AR2 site carried on a 46 bp fragment from the DAN1 promoter activated lacZ transcription under anaerobic conditions, as did a 26 bp synthetic AR2 fragment fused to MEL1. Nucleotide substitutions within the AR2 sequence eliminated the activity of the 46 bp segment. Ablation of the AR2 sequences in the full promoter caused a partial reduction of expression. The presence of the ATTGTT core (recognized by HMG proteins) in the AR2 sequence suggests that an HMG protein may activate through AR2. One region was implicated in aerobic repression of DAN1. It contains sites for the heme-induced Mot3 and Rox1 repressors.

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Microbial pathogens have evolved many ingenious ways to infect their hosts and cause disease, including the subversion and exploitation of target host cells. One such subversive microbe is enteropathogenic Escherichia coli (EPEC). A major cause of infantile diarrhea in developing countries, EPEC poses a significant health threat to children worldwide. Central to EPEC-mediated disease is its colonization of the intestinal epithelium. After initial adherence, EPEC causes the localized effacement of microvilli and intimately attaches to the host cell surface, forming characteristic attaching and effacing (A/E) lesions. Considered the prototype for a family of A/E lesion-causing bacteria, recent in vitro studies of EPEC have revolutionized our understanding of how these pathogens infect their hosts and cause disease. Intimate attachment requires the type III-mediated secretion of bacterial proteins, several of which are translocated directly into the infected cell, including the bacteria's own receptor (Tir). Binding to this membrane-bound, pathogen-derived protein permits EPEC to intimately attach to mammalian cells. The translocated EPEC proteins also activate signaling pathways within the underlying cell, causing the reorganization of the host actin cytoskeleton and the formation of pedestal-like structures beneath the adherent bacteria. This review explores what is known about EPEC's subversion of mammalian cell functions and how this knowledge has provided novel insights into bacterial pathogenesis and microbe-host interactions. Future studies of A/E pathogens in animal models should provide further insights into how EPEC exploits not only epithelial cells but other host cells, including those of the immune system, to cause diarrheal disease.

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This layer is a georeferenced raster image of the historic paper map entitled: Carte de l'isle de Cheu-Chan, ou l'isle de Chusan, de la province de Che-kiang : avec les costes et isles voisines, tiré de Thornton, Hydrog. anglois et autres navigatrs. par N.B., Ingr. de la Marine ; J.V. Schley direx. It was published by Pierre de Hondt in 1749. Scale [ca. 1:400,000]. Covers the Zhoushan Qundao region, China. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Universal Transverse Mercator (UTM Zone 51N, meters, WGS 1984) projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, shoreline features (islands, rocks, anchorage points, etc.), and more. Relief shown pictorially. Depths shown by soundings.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image of the historic paper map entitled: Plan de la ville de Hang-Tcheou-Fou ou Hang-Chew-Fu, capitale de la province de Che-kiang : tiré du P. du Halde, J.V. Schley direx. It was published by Pierre de Hondt in 1749. Scale [ca. 1:29,500]. Covers Hangzhou, China. Map in French and Dutch. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Universal Transverse Mercator (UTM Zone 51N, meters, WGS 1984) projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, canals, selected buildings, fortification, shoreline features, and more. Relief shown pictorially.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image of the historic paper map entitled: Carte de la Tartarie Occidentale : pour servir a l'Histoire generale des voyages, tiré des auteurs anglois, par N. Bellin, Ingenieur de la Marine. It was published by Pierre de Hondt in 1749. Scale [ca. 1:5,400,000]. Covers Mongolia and North China, and portion of Russia. Map in French and Dutch. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Asia North Lambert Conformal Conic coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, roads, the Great Wall of China, and more. Relief shown pictorially.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image of the historic paper map entitled: Carte de la coste d'Arabie, Mer Rouge et Golfe de Perse : tir�e de la Carte de l'Oc�an Oriental publi�e en 1740 par Ordre de Mgr le Comte de Maurepas augment�e sur des remarques particuli�res et dress�e sur des observations astronomiques = Kaart van de Kust van Arabi�, de Roode-Zee en de Gulf van Persi� Gemaakt na de Fransse-Kaart van den Ooster-Ocean uitgegeven A. 1740 op Bevel van den Hre Grave de Maurepas Vermeederd op byzondere Aanmerkingen, en geschikt volgens Sterrekundige-Waarnemingen, J. V. Schley. It was published by Pierre de Hondt between 1747 and 1767. Scale [ca. 1:14,000,000]. Covers the Arabian Peninsula. Map in French and Dutch. The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Gall Stereographic projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, shoreline features, and more. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection as part of the Open Collections Program at Harvard University project: Islamic Heritage Project. Maps selected for the project represent a range of regions, originators, ground condition dates, scales, and purposes. The Islamic Heritage Project consists of over 100,000 digitized pages from Harvard's collections of Islamic manuscripts and published materials. Supported by Prince Alwaleed Bin Talal and developed in association with the Prince Alwaleed Bin Talal Islamic Studies Program at Harvard University.

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This layer is a georeferenced raster image of the historic paper map entitled: Tiré de l'ouvrage les missions catholiques d'Afrique en 1889, par le Baron Léon Bethune. It was published by Société de St. Augustin in 1889. Scale 1:20,000,000. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to a non-standard 'World Sinusoidal' projection with the central meridian at 20 degrees east. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, colonial possessions, ecclesiastical districts, and more. Relief is shown by hachures. Includes index.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection and the Harvard University Library as part of the Open Collections Program at Harvard University project: Organizing Our World: Sponsored Exploration and Scientific Discovery in the Modern Age. Maps selected for the project correspond to various expeditions and represent a range of regions, originators, ground condition dates, scales, and purposes.

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This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 1 entitled: Lutece, ou, premier plan de la ville de Paris: tiré de Cesar, de Strabon, de l'empereur Iulien, et d'Ammian Marcellin. The map represents Paris ca. 4th Century. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, selected buildings, ground cover, and more. Relief shown by hachures. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 2 entitled: Lutece conquise par les François sur les Romains, ou, second plan de la ville de Paris: tiré du misopogone de l'empereur Iulien, d'Amian Marcellin, de Gregoire de Tours, de Boece, de l'abbé Suger, des ancients titres, du tresor des chartres, des archives de St. Germain, de St. Magloire, de St. Eloy, du temple et des vestiges de cette ancienne enceinte qui subsiste encore au jourd'huy ou l'on a vû de nos jours. The map represents Paris, 5th to 10th Centuries. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, built-up areas and selected buildings, fortification, ground cover, and more. Relief shown by hachures.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 3 entitled: Troisieme plan de la ville de Paris son êtendiie et les bourgs dont elle êtoit environnée sous le regne de Louis le Jeune VIIe du nom: tiré des descriptions de Fortunat, de Gregoire de Tours, Dabbon, et D'Aimoin, et des anciens titres qui sont dans les Tresor des chartres de France, les registres de la Chambre, des Comptes, les Banieres du Châtelet de Paris, les Archives de l'Archevêché celles de Saint Germain, de Ste. Genevieve, de St. Martin, de S. Eloy, et du Temple. The map represents Paris, 1137 to 1180. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, built-up areas and selected buildings, fortification, ground cover, and more. Relief shown by hachures.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 4 entitled: Quatriême plan de la ville de Paris son accroissement, et l'etat ou elle êtoit sous le regne de Philippe Auguste qui mourut l'an 1223 apres avoir regné 43 ans: tiré de Rigord, de Knobelsderf, de Rodolphe Boterée, de Raoul, de Praesles, de Paul Merula, de Guaquin, de Pithou, de Papire Masson, de Corrozet, de Dubreüil, de Duchesne, des memoriaux, et autres anciens registres de la Chambre, des Comptes, et des archives de l'Archevêché du Chaptire de N. Dame, et des anciennes abbayes. The map represents Paris, 1180 to 1223. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, built-up areas and selected buildings, fortification, ground cover, and more. Relief shown by hachures. Includes index.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 5 entitled: Cinquiême plan de la ville de Paris son accroissement et sa quatriême clôture commancée sous Charles V. l'an 1367 et finie sous Charles VI. l'an 1383: tiré des devis et marchez faits avec le ouvriers, des procez verbaux de toisez et receptions des ouvrages des comptes rendus par ceux qui en eurent la conduite, de la chronique m.s. de St. Denis et d'autres titres et manuscrits qui sont conservez en la Chambre des Comptes et dans Biblioteques. The map represents Paris, 1367 to 1383. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, built-up areas and selected buildings, fortification, ground cover, and more. Relief shown by hachures. Includes index, text, and notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 7 entitled: Septiême plan de la ville de Paris son acroissement [sic] et ses embelissemens sous Henry III. et Louis XIII. depuis 1589 jusqu'en 1643: tiré des lettres patentes ou arrest du conseil qui ont ordonné les ouvrages des devis et marchez faits avec les entrepreneurs et levé sur les lieux ou ils ont été construits, et ou la plus grand partie subsistent encore. The map represents Paris, 1589 to 1643. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, built-up areas and selected buildings, fortification, ground cover, and more. Relief shown by hachures. Includes index, text, and notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 6 entitled: Sixiême plan de la ville de Paris et ses accroissements depuis le commencement du régne de Charles VII. l'an 1422 jusqu'a la fin du régne d'Henry III. l'an 1589: tiré des lettres patentes qui ont ordonné les ouvrages, des contrats passez avec les entrepreneurs, des registres de la chambre des comptes de l'histoire et des memoires du temps. The map represents Paris, 1422 to 1589. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, built-up areas and selected buildings, fortification, ground cover, and more. Relief shown by hachures. Includes index, text, and notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

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[ʻAbd al-Vahāb ibn Muḥammad Amīn al-Shāhshahānī].