Antibacterial and cytotoxic activity of Kenyan medicinal plants

Seven medicinal plant extracts traditionally used in Kenya, mainly for management of infectious conditions, were chosen and screened for their antibacterial activity against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Bacillus cereus and Staphylococcus aureus) bacteria. Antibacterial activity was tested using the broth dilution method. Harrisonia abyssinica and Terminalia kilimandscharica extracts showed significant activity against Gram+ and Gram- bacteria. The methanolic extracts of T. kilimandscharica bark and H. abyssinica bark and leaves showed minimum inhibitory activity against all tested bacteria, with minimal inhibitory concentrations ranging from 25-150 mg/mL. Ajuga remota and Amaranthus hybridus, which are lethal to brine shrimp nauplii, showed significantly lower antibacterial activity than those that were relatively non-toxic.

Biological activity of neosergeolide and isobrucein B (and two semi-synthetic derivatives) isolated from the Amazonian medicinal plant Picrolemma sprucei (Simaroubaceae)

In the present study, in vitro techniques were used to investigate a range of biological activities of known natural quassinoids isobrucein B (1) and neosergeolide (2), known semi-synthetic derivative 1,12-diacetylisobrucein B (3), and a new semi-synthetic derivative, 12-acetylneosergeolide (4). These compounds were evaluated for general toxicity toward the brine shrimp species Artemia franciscana, cytotoxicity toward human tumour cells, larvicidal activity toward the dengue fever mosquito vector Aedes aegypti, haemolytic activity in mouse erythrocytes and antimalarial activity against the human malaria parasite Plasmodium falciparum. Compounds 1 and 2 exhibited the greatest cytotoxicity against all the tumor cells tested (IC50 = 5-27 µg/L) and against multidrug-resistant P. falciparum K1 strain (IC50 = 1.0-4.0 g/L) and 3 was only cytotoxic toward the leukaemia HL-60 strain (IC50 = 11.8 µg/L). Quassinoids 1 and 2 (LC50 = 3.2-4.4 mg/L) displayed greater lethality than derivative 4 (LC50 = 75.0 mg/L) toward A. aegypti larvae, while derivative 3 was inactive. These results suggest a novel application for these natural quassinoids as larvicides. The toxicity toward A. franciscana could be correlated with the activity in several biological models, a finding that is in agreement with the literature. Importantly, none of the studied compounds exhibited in vitro haemolytic activity, suggesting specificity of the observed cytotoxic effects. This study reveals the biological potential of quassinoids 1 and 2 and to a lesser extent their semi-synthetic derivatives for their in vitro antimalarial and cytotoxic activities.

In vitro antiplasmodial activity and toxicity assessment of plant extracts used in traditional malaria therapy in the Lake Victoria Region

As part of our program screening the flora of the Lake Victoria Region, a total of 54 organic extracts from seven plant families (8 species) were individually tested for antiplasmodial activity against chloroquine-sensitive [Sierra Leone (D-6)] and chloroquine-resistant [Vietnam (W-2)] strains. Only 22% of these extracts exhibited very high in vitro antiplasmodial activity. Six methanol (MeOH) extracts and one chloroform extract showed in vitro antiplasmodial activity against the D-6 Plasmodium falciparum strain, while only three MeOH extracts were active against the W-2 strain. All of the ethyl acetate extracts proved to be inactive against both strains of P. falciparum. A brine shrimp cytotoxicity assay was used to predict the potential toxicity of the extracts. The cytotoxicity to antiplasmodial ratios for the MeOH extracts were found to be greater than 100, which could indicate that the extracts are of low toxicity.

Análisis de la dimensión social y económica de la relación de la comunidad de Cumbe con el manglar y los impactos de la industria camaronera

En los últimos años la industria camaronera ha adquirido mucha importancia en Brasil y genera presiones sobre ecosistemas y comunidades tradicionales que viven en equilibrio con su entorno. En Cumbe, una comunidad tradicional del municipio de Aracati, Ceará, Brasil, el ecosistema que rodea a su población es el manglar. Debido a esta fuerte presión, tanto el manglar como los servicios ambientales que la población local se beneficia, están transformándose. En este trabajo, la población local de Cumbe identifica, valora y caracteriza los servicios ambientales que les proporciona el manglar dándole así una vital importancia, que puede servir de precedente para una posible protección. Por otra parte, se hace una aproximación en términos económicos de la pesca artesanal y las granjas de camarón para discutir a corto y a largo plazo cuál es el modelo más sostenible a seguir.

Evaluación ambiental de la acuicultura de camarón sobre el ecosistema manglar en el tramo bajo del río Juguaribe

En los últimos años la acuicultura de camarón se ha desarrollado como industria potencial en Brasil y sus actividades generan presiones sobre ecosistemas y comunidades tradicionales que viven en equilibrio con su entorno. En Cumbe, una comunidad tradicional del municipio de Aracati, Ceará, Brasil, el ecosistema que rodea a su población es el manglar. Debido a esta fuerte presión, tanto el manglar como los servicios ambientales de los que la población local se beneficia, están en peligro. Este estudio pretende evaluar las diferencias entre tres áreas de manglar que están afectadas en diferente grado por la presión de las industrias camaroneras. Se evalúan parámetros biológicos y la estructura de los manglares para observar las posibles diferencias y relacionarlas con las diferentes variables.

L'altra cara del progrés. Estudi multidimensional sobre les conseqüències de les activitats d'explotació intensiva sobre l'ecosistema del manglar i les activitats econòmiques tradicionals a la comunitat de Cumbe, Brasil

La localitat de Cumbe és una comunitat tradicional que es troba al tram baix del riu Jaguaribe situat a l’estat de Ceará (Brasil) i que ha viscut centenars d’anys a partir de les pràctiques extractives que es porten a terme a l’ecosistema del manglar. Fa 20 anys va aparèixer a la regió una nova economia basada en l’explotació intensiva. L’aqüicultura destinada a la cria de gamba està causant danys severs a l’ecosistema del manglar i perjudicant greument la font d’ingressos de moltes famílies, amenaçant la continuïtat de l’economia local i deteriorant la qualitat de vida de les persones que s’hi dediquen. A partir de l’observació personal i la immersió en aquesta societat s’ha detectat que l’economia tradicional està infravalorada pel govern i per les persones que donen suport a l’aqüicultura. Per aquest motiu s’han creat una sèrie d’indicadors, a partir de les activitats extractives del cranc, que posin en valor aquesta economia tradicional envers la nova economia emergent.

Propuesta para un Proyecto de Evaluación de Stocks de Camarón de Profundidad en aguas de Cabo Verde (2010-2013)

A bibliographic revision on the fisheries research in the Canary, Madeira, and the Azores Islands related to stock assessment of the striped soldier shrimp (Plesionika edwardsii) (Crustacea, Decapoda, Pandalidae) was made. Biological information –reproduction, growth, mortality, recruitment– of the target species, selective technological developments –multiple semi-floating shrimp traps–, and know-how gained by the ICCM can give support to elaborate and execute an ICCM-INDP joint project, in order to explore and assess its insular stocks in the Capeverdian deepwaters (150-300 m). In the frame of the UE Trans-national Cooperation Programme MAC (2007- 2013), the next coming call for proposals seems to be an adequate source to fund it. This project should include some activities for the valorisation and promotion of this new shellfish product, as well as for scientific-technical and social divulgation, and for giving advice to the decision-makers. Lastly, with the aim of developing a new fishing activity in the Cape Verdes on a responsible and sustainable basis, authors have scheduled a second study phase. This should consist of a process of technology transfer, followed by an experimental fishing pilot project including a study on biological and commercial viability with the participation of the Capeverdian fishing sector.

Variscan lamprophyres in the Lower Penninic domain (Central Alps): age and tectonic significance

Lamprophyre dykes have been recently discovered in blocks of gneiss embedded in a calcschist formation of wildflysch type that forms the top of the Mesozoic-Tertiary metasedimentary cover of the Antigorio nappe (the Teggiolo zone) in the Val Bavona (Lower Penninic, NW Ticino, Switzerland). The presence of the lamprophyres gives a clue to the possible source of these blocks. Similar dykes occur in the N part of the Maggia nappe where they are intruded into the Matorello granite and the surrounding gneisses. We studied these lamprophyres at two localities in the Teggiolo zone (Tamierpass and Lago del Zott) and at one locality in the Maggia nappe (Laghetti). Detailed mineralogical and geochemical investigations confirm their great similarity, particularly between the Tamier and Laghetti dykes. They all recrystallized during Alpine metamorphism under amphibolite facies conditions and lost their primary mineral assemblages and textures. The chemistry reveals a calc-alkaline affinity, a limited differentiation range, features of mineral accumulation and intense remobilization in some cases. The lamprophyres are characterized by a high mg# and relatively low contents in REE and other incompatible elements. In situ SHRIMP and LA-ICPMS U-Pb zircon dating yielded ages of 284.8 +/- 1.7 Ma (Tamier), 290.0 +/- 1.3 Ma (Zott) and 290.5 +/- 3.7 Ma (Laghetti). These ages are compatible with the general late- to post-Variscan magmatic evolution of the Helvetic and Lower Penninic domains. The lamprophyres are considered as melts derived from the lithospheric Variscan mantle, variously hybridized and differentiated at the contact with crustal material during late- to post-orogenic extension. These lamprophyres are chemically distinct from earlier lamprophyres of Visean age, emplaced together with their associated granites in transcurrent fault zones during the Variscan orogenic compression. The similarity of these different dykes suggests that the front of the Maggia nappe is a likely source of the gneissic blocks embedded in the calcschists at the top of the Teggiolo zone. They would have been provided by the advancing Maggia nappe during its thrusting over the Antigorio nappe and simultaneous closure of the Teggiolo sedimentary basin.

Monitoreo de postlarvas de langostinos en Tumbes, Perú. 1996-2003

Durante 1996-2003 se realizó el monitoreo de postlarvas de langostinos en el litoral y los canales de marea de Tumbes donde se identificaron cinco especies: Litopenaeus vannamei, L. stylirostris, L. occidentalis farfantepenaeus californiensis y F. brevirostris.

Lipid reserves of red mullet (Mullus barbatus) during pre-spawning in the northwestern Mediterranean

Lipid reserves are a particularly important attribute of fishes because they have a large influence on growth, reproduction and survival. This study analyses the lipid content of red mullet (Mullus barbatus) pre-spawners in three different areas of the northwestern Mediterranean in relation to trawling activities and river runoff. The muscle lipid was considered as an indicator of the somatic condition of individuals whilst the gonad lipid was used as a proxy of the energy invested in reproduction. The results show that fish with the highest muscle lipid levels inhabited the area where fishing impact was lowest. Since the abundance and biomass of polychaetes, which represent the main food source for red mullet, were found to be lower in trawled zones than in unfished ones, we suggest that differences in the muscle lipid levels between areas might be attributed to variation in prey abundance in relation to fishing impact. However, no impact of river runoff on lipid reserves of red mullet was observed. The results also show that muscle and gonad lipid reserves are not related to each other during pre-spawning

Camarón de río Cryphiops caementarius (Molina, 1782) en la costa centro-sur del Perú, 2007

Entre julio y diciembre del 2007, se efectuó el Monitoreo Poblacional del Cryphiops caementarius de los ríos Cañete, Ocoña, Majes-Camaná y Tambo. La calidad del agua evidenció alteraciones en los parámetros fisicoquímicos con respecto al periodo 1996-2007. El río Majes-Camaná alcanzó los mayores valores de densidad (1,87 ind/m2) y biomasa media (21,51 g/m2), en el río Cañete hubo reducción en la densidad (0,25 ind/m2) y biomasa media (2,28 g/m2) que coincidió con mayor número de ejemplares menores a 70 mm.

Differences in diet between the two largest breeding colonies of Audouin's gulls: the effects of fishery activities

We analysed and compared the diet of Audouin´s gulls Larus audouinii between their two largest breeding sites in the world: the Ebro Delta and the Chafarinas Islands (western Mediterranean). These two localities showed marked differences in the features of the commercial fishing fleet: in the Ebro Delta area a large fishing fleet produced large amounts of discards, while in the Chafarinas the fleet discarded smaller amounts of fish and marine invertebrates, due to the smaller number of vessels. It is also likely that the percentage of discards from total catches is also lower around the Chafarinas than at the Ebro Delta. We distinguished two types of fishing to compare diet compositions: diurnal (only trawling activity) and diurnal and nocturnal (trawling and purse-seine activity, respectively). We also differentiated regurgitates from young nestlings (up to 20 days old) and from older nestlings or adult birds. At the two localities, fish was the main food of Audouin´s gulls, with epipelagic prey (mainly clupeoids) being more important when both diurnal and nocturnal fisheries were operating. This confirms that epipelagic prey either caught actively by the gulls or linked to fisheries was particularly important in the feeding habits of Audouin´s gulls. Nevertheless, differences between the two colonies appear mainly when only trawlers operated: while at the Ebro Delta gulls showed higher consumption of benthic-mesopelagic prey (probably linked to a higher trawler discard availability), gulls from the Chafarinas Islands consumed higher biomass of epipelagic prey probably caught actively at night. When both fleets operated around the two colonies, the average biomass of prey in a regurgitate of younger chicks was significantly higher at the Ebro Delta than at Chafarinas, and the opposite trend was recorded for older nestlings and adults. Niche width was broader in Chafarinas than in the Ebro Delta for both age classes and for any fishing fleet schedule, suggesting again that the exploitation of discards was higher at the Ebro Delta than at the Chafarinas, where gulls showed a more varied diet. Despite the fact that availability of discards was probably higher at the Ebro Delta than at Chafarinas, the per capita availability was not so different at both localities due to the increasing seabird community population at the Ebro Delta, which ca. doubled that at Chafarinas in the last decade.

Differences in diet between the two largest breeding colonies of Audouin's gulls: the effects of fishery activities

We analysed and compared the diet of Audouin´s gulls Larus audouinii between their two largest breeding sites in the world: the Ebro Delta and the Chafarinas Islands (western Mediterranean). These two localities showed marked differences in the features of the commercial fishing fleet: in the Ebro Delta area a large fishing fleet produced large amounts of discards, while in the Chafarinas the fleet discarded smaller amounts of fish and marine invertebrates, due to the smaller number of vessels. It is also likely that the percentage of discards from total catches is also lower around the Chafarinas than at the Ebro Delta. We distinguished two types of fishing to compare diet compositions: diurnal (only trawling activity) and diurnal and nocturnal (trawling and purse-seine activity, respectively). We also differentiated regurgitates from young nestlings (up to 20 days old) and from older nestlings or adult birds. At the two localities, fish was the main food of Audouin´s gulls, with epipelagic prey (mainly clupeoids) being more important when both diurnal and nocturnal fisheries were operating. This confirms that epipelagic prey either caught actively by the gulls or linked to fisheries was particularly important in the feeding habits of Audouin´s gulls. Nevertheless, differences between the two colonies appear mainly when only trawlers operated: while at the Ebro Delta gulls showed higher consumption of benthic-mesopelagic prey (probably linked to a higher trawler discard availability), gulls from the Chafarinas Islands consumed higher biomass of epipelagic prey probably caught actively at night. When both fleets operated around the two colonies, the average biomass of prey in a regurgitate of younger chicks was significantly higher at the Ebro Delta than at Chafarinas, and the opposite trend was recorded for older nestlings and adults. Niche width was broader in Chafarinas than in the Ebro Delta for both age classes and for any fishing fleet schedule, suggesting again that the exploitation of discards was higher at the Ebro Delta than at the Chafarinas, where gulls showed a more varied diet. Despite the fact that availability of discards was probably higher at the Ebro Delta than at Chafarinas, the per capita availability was not so different at both localities due to the increasing seabird community population at the Ebro Delta, which ca. doubled that at Chafarinas in the last decade.

Avaluació dels canvis de ritme de sedimentació en el canyó submarí d'Arenys a partir del Pb-210

Amb la finalitat d’estudiar els ritmes d’acumulació de sediments durant els últims 100 anys, s’han extret tres testimonis de sediments del canyó d’Arenys a profunditats de 1074 m, 1410 m i 1632 m respectivament. Els ritmes de sedimentació basats en els perfils verticals de Pb-210 suggereixen que les tendències actuals sobre el flux i acumulació de sediments poden ser diferents a tendències passades. Durant la dècada dels 70 es va portar a terme una ràpida evolució de la flota pesquera del port d’Arenys de Mar. Aquest fet es pot relacionar amb els canvis en el ritme d’acumulació dels sediments al testimoni extret a 1074 m. Els flancs del canyó submarí són objectiu dels arrossegadors del port d’Arenys de Mar, una activitat que pot fer variar la morfologia del fons marí, la resuspensió de les partícules i pot crear fluxos de terbolesa. Per tant, els resultats suggereixen que l’activitat pesquera d’arrossegament pot afectar als ambients submarins d’una manera més important del que s’havia pensat.

Influence on present-day coastal dynamics and evolution of a relict subaqueous delta lobe: Sol de Riu lobe, Ebro Delta. Continental Shelf Research, 74: 94-104. doi:10.1016/j.csr.2013.11.021

We used high-resolution swath-bathymetry data to characterise the morphology of the abandoned subaqueous Sol de Riu delta lobe in the Ebro Delta, Western Mediterranean Sea. This study aims to assess the influence of an abandoned delta lobe on present-day coastal dynamics in a micro-tidal environment. Detailed mapping of the relict Sol de Riu lobe also showed a set of bedforms interpreted as footprints of human activities: seasonal V-shaped depressions on the middle shoreface due to boat anchoring and old trawling marks between 16 and 18 m water depth. Estimations of the mobility of bottom sediment showed that the shallowest shoreface (i.e. less than 7 m depth) is the most dynamic part of the relict lobe, while the middle shoreface experienced significant morphological changes since the lobe was abandoned. The deepest shoreface (i.e. water depth in excess of 15 m), which corresponds to the front of the lobe, is defined by a very small potential for morphological change. Simulations showed that while the relict lobe does not significantly affect the typical short period waves (Tp ≈4 s) in the study area, it does interfere with the most energetic wave conditions (Tp ≥ 7 s) acting as a shoal leading to the concentration of wave energy along the shoreline northwest of the lobe. The consequence of such modification of the high-energy wave propagation pattern by the relict lobe is an alteration of the wave-induced littoral sediment dynamics with respect to a situation without the lobe.