899 resultados para immunomagnetic separation – IMS
Resumo:
Orthogonal design and uniform design were used for the optimization of separation of enantiomers using 2,6-di-O-methyl-beta-cyclodextrin (DM-beta-CD) as a chiral selector by capillary zone electrophoresis, The concentration of DM-beta-CD, buffer pH, running voltage, and capillary temperature were selected as variable parameters, their different effects on peak resolution were studied by the design methods. It was concluded that orthogonal design offers a rapid and efficient means for testing the importance of individual parameters and for determining the optimum operating conditions. However, for a large number of both factors and levels, uniform design is more efficient, The effect of addition of methanol and citric acid buffer on the separation of enantiomers was also examined.
Resumo:
Direct enantiomeric separation of all four optical isomers of 2-phenylcyclopropane carboxylate ester was first achieved on each of the three different beta-cyciodextrin chiral stationary phases (CSPs) in GC. Using these CSPs, enantiomeric excess of the products of enantioselective cyclopropanation can be determined directly, conveniently and fast.