Desempenho de Ankistrodesmus gracilis (Reischi) Korsikov (Chlorophyta) cultivada em três meios de cultura em laboratório

Pós-graduação em Aquicultura - FCAV

Production and characterization of lipases and immobilization of whole cell of the thermophilic Thermomucor indicae seudaticae N31 for transesterification reaction

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Biological studies of puccina species using germinatelia

Despite the importance of plant pathogens belonging to the Order Pucciniales (=Uredinales), popularly called rusts, certain peculiarities of its biology is poorly known, mainly due to the difficulty of growing them in culture media and also by the lack of appropriate methodologies for the development of research. Therefore, this study was to verify the efficiency of apparatus called germinatelia to obtain data about the basidiospores production biology of different Pucciniales species. Were used in the study Puccinia pampeana, P. psidii, P. pelargonii-zonalis, P. arachidis, P. mogiphanis, P. emiliae, P. hetereospora, P. malvacearum and P. cnici-oleracei. The use of germinatelia have revealed high basidiospores production for a period up to 5 months at temperatures between 12 degrees and 21 degrees C. Furthermore, in P. malvacearum was possible to record the germination by repetition (survival strategy) in 4.5% of basidiospores produced. All species were favored to mild temperatures. These results therefore demonstrate that the use of germinatelios is efficient, assisting in obtaining data about the biology and cycles of different Puccinia species, enabling better understanding of these pathogens, resulting in management and control measures most appropriate/accurate.

Remoção de N-amoniacal em baixas concentrações de oxigênio dissolvido a partir do efluente de frigorífico bovino

This work aimed to develop a biological system for removal of ammonia nitrogen operating at low concentrations of dissolved oxygen. Thus, a biological upflow vertical reactor was built, in which the affluent pass through the support media until the top. Sludge from an anaerobic stabilization pond of a slaughterhouse unit in the city of Presidente Prudente - SP was used as inoculum. Initially the system operated in batch and afterwards in a continuous flow with different HRT. For feeding the reactor, an initial phases was adopted a synthetic culture media, described by Martins (2007), in order to establish the ideal conditions for the development of Anammox bacteria and subsequently, submitted to the system a slugde effluent of slaughterhouse. The results showed significant removal efficiency of N-NH4+, especially in the phase without recirculation of culture media, with an average of 71% removal, with the proportion of removal of N-NH4 +:N-NO2- average 1: 1,69. For the period of operation with effluent from the slaughterhouse, were not obtained satisfactory results, without confirmation of the proliferation of Anammox bacteria in the system, due to the high presences of organic matter in the same confirmed by high concentrations of COD

Purification and properties of polygalacturonase produced by thermophilic fungus Thermoascus aurantiacus CBMAI-756 on solid-state fermentation

Polygalacturonases are enzymes involved in the degradation of pectic substances, being extensively used in food industries, textile processing, degumming of plant rough fibres, and treatment of pectic wastewaters. Polygalacturonase (PG) production by thermophilic fungus Thermoascus aurantiacus on solid-state fermentation was carried out in culture media containing sugar cane bagasse and orange bagasse in proportions of 30% and 70% (w/w) at 45°C for 4 days. PG obtained was purified by gel filtration and ion-exchange chromatography. The highest activity was found between pH 4.5 and 5.5, and the enzyme preserved more than 80% of its activity at pH values between 5.0 and 6.5. At pH values between 3.0 and 4.5, PG retained about 73% of the original activity, whereas at pH 10.0 it remained around 44%. The optimum temperature was 60–65°C. The enzyme was completely stable when incubated for 1 hour at 50°C. At 55°C and 60°C, the activity decreased 55% and 90%, respectively. The apparent molecular weight was 29.3 kDa, Km of 1.58 mg/mL and Vmax of 1553.1μmol/min/mg. The presence of Zn+2, Mn+2, and Hg+2 inhibited 59%, 77%, and 100% of enzyme activity, respectively. The hydrolysis product suggests that polygalacturonase was shown to be an endo/exoenzyme.

Análise microbiológica da exposição das roscas dos implantes osseointegráveis no seio maxilar de ovelhas

Pós-graduação em Odontologia - FOA

Cenário e perspectivas para a melhoria do ensino público no Brasil: mídia, cultura e educação

Thinking about improving public education in Brazil means thinking not only on what we wish to develop, skills and abilities in individuals, but understand and clearly identify the type of citizens we are devoloping and what kind of society we want for the future. In this context it is important to note that the practice in the classroom today can not be considered without regard to education steeped in culture and, therefore, in the forms of modern communication. So if there is a crisis in education, it can not be solved only within the classroom. The challenge for school education is to show students within the classroom how they will be out of school. Quality education is a blueprint for the future, to the unknown, to a world we do not know now. In this sense, to direct the education only for the labor market is a mistake, because the market is so today, but may be another in ten or fifteen years. Precisely because we do not know what are the requirements for citizens of the future, we must focus on what is essential in the formation of our youth. Is essential then to understand the ideas, learn to discuss them, argue and learn to recognize the role of technology in social and cultural evolution of mankind.

Color alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocols

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Patógenos periodontais: comparação entre cultura bacteriana e PCR em tempo real para teste diagnóstico

The correct distinguishment of microorganisms involved in the periodontal disease pathogen, it is important in the understanding of its progression and adequate treatment planning. Considering this fact, some molecular methods of identification and quantification were developed and are extremely sensitive and precise in the characterization of different bacteria species. The present study aimed to realize a literature review, including studies that realized a comparative analysis between bacterial culture and real time PCR methods in the identification of pathogens. The bacterial culture method can possibly identify new microorganisms and realize antibiotics sensitivity tests. The real time PCR is a microbiologic test that identifies and quantifies bacterial species, through gene amplification of predetermined DNA fragments, with high sensitivity and specificity, and need a shorter operation time of the operator when compared to the bacterial culture method. In this way, to determine a specific diagnostic test, should be considered not only its precision in the identification of microorganisms, but the cost-benefit relationship as well.

Artificial neural network associated to UV/Vis spectroscopy for monitoring bioreactions in biopharmaceutical processes

Currently, mammalian cells are the most utilized hosts for biopharmaceutical production. The culture media for these cell lines include commonly in their composition a pH indicator. Spectroscopic techniques are used for biopharmaceutical process monitoring, among them, UV–Vis spectroscopy has found scarce applications. This work aimed to define artificial neural networks architecture and fit its parameters to predict some nutrients and metabolites, as well as viable cell concentration based on UV–Vis spectral data of mammalian cell bioprocess using phenol red in culture medium. The BHK-21 cell line was used as a mammalian cell model. Off-line spectra of supernatant samples taken from batches performed at different dissolved oxygen concentrations in two bioreactor configurations and with two pH control strategies were used to define two artificial neural networks. According to absolute errors, glutamine (0.13 ± 0.14 mM), glutamate (0.02 ± 0.02 mM), glucose (1.11 ± 1.70 mM), lactate (0.84 ± 0.68 mM) and viable cell concentrations (1.89 105 ± 1.90 105 cell/mL) were suitably predicted. The prediction error averages for monitored variables were lower than those previously reported using different spectroscopic techniques in combination with partial least squares or artificial neural network. The present work allows for UV–VIS sensor development, and decreases cost related to nutrients and metabolite quantifications.

Avaliação do potencial de degradação do herbicida diuron por fungos isolados de solo de canavial e suas tolerâncias aos metabólitos gerados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de ácidos orgânicos por cepas leveduras assimiladoras de xilose

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Conventional and whitening toothpastes: Cytotoxicity, genotoxicity and effect on the enamel surface

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência do manejo do solo em atributos químicos, físicos e microbiológicos dos córregos Jaboticabal e Cerradinho

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Susceptibilidade antimicrobiana ao metronidazole de microrganismos isolados de periodontite crônica e agressiva

The objective of this study was to evaluate the microbial susceptibility to metronidazole from microorganisms isolated from patients with chronic or aggressive periodontitis. The samples were obtained from 50 patients with periodontitis and microorganisms were isolated onto selective and nonselective culture media, identified by biochemical methods and tested for susceptibility to metronidazole. The results evidenced the susceptibility to metronidazole among gram-negative anaerobes and some intermediate susceptibility and resistance among Grampositive anaerobes and facultatives.