In vitro activity of Artemisia annua L (Asteraceae) extracts against Rhipicephalus (Boophilus) microplus

A atividade de extratos vegetais sobre parasitas pode indicar grupos de substâncias de uso potencial no controle de Rhipicephalus (Boophilus) microplus. O objetivo do presente estudo foi investigar a ação in vitro de extratos de Artemisia annua sobre esta espécie. A concentração das lactonas sesquiterpênicas artemisinina e deoxiartemisinina presentes nos extratos vegetais, foi quantificada via cromatografia líquida de alta eficiência. Quatro extratos produzidos a partir do extrato bruto concentrado (EBC) foram avaliados sobre larvas pela metodologia do papel impregnado, com leitura após 24 horas de incubação. As fêmeas ingurgitadas foram imersas por cinco minutos no EBC e nos seus quatro extratos derivados, e incubadas para posterior análise dos parâmetros biológicos. Os extratos não tiveram eficácia sobre as larvas nas concentrações avaliadas (de 3,1 a 50 mg.mL-1). O EBC apresentou melhor eficácia sobre as fêmeas ingurgitadas (CE 50 de 130,6 mg.mL-1 e CE 90 de 302,9 mg.mL-1) que os extratos derivados. Esses resultados tendem a confirmar que a ação da artemisinina sobre as fêmeas ingurgitadas de R. (B.) microplus estaria condicionada à sua ingestão através do sangue. Nesse caso, os métodos in vitro seriam inadequados para a efetiva avaliação da ação de A. annua R.(B.) microplus.

Physical activity and postural control in the elderly: Coupling between visual information and body sway

Background: Aging is characterized by a decline in the postural control performance, which is based on a coherent and stable coupling between sensory information and motor action. Therefore, changes in postural control in elderlies can be related to changes in this coupling. In addition, it has been observed that physical activity seems to improve postural control performance in elderlies. These improvements can be due to changes in the coupling between sensory information and motor action related to postural control. Objective: the purpose of this study was to verify the coupling between visual information and body sway in active and sedentary elderlies. Methods: Sixteen sedentary elderlies ( SE), 16 active elderlies ( AE) and 16 young adults ( YA) were asked to stand upright inside a moving room in two experimental conditions: ( 1) discrete movement and ( 2) continuous movement of the room. Results: In the continuous condition, the results showed that the coupling between the movement of the room and body sway was stronger and more stable for SE and AE compared to YA. In the discrete condition, SE showed larger body displacement compared to AE and YA. Conclusions: SE have more difficulty to discriminate and to integrate sensory information than AE and YA indicating that physical activity may improve sensory integration. Copyright (C) 2005 S. Karger AG, Basel.

Environmental education and the health risk question: how do we educate the educators working with this theme?

Our society is presently seeing a trend of continuous increase of risk with severe consequences, especially those linked to environmental aspects. It has obligated us to reflect on this production and its effects, either positive or negative, and, after a long reflection, to think about the adopted model of civilization and the possibilities of changing such a model. The educational institution is among the social departments responsible for this revision process, since it represents one possibility to promote a more critical vision from society, providing it with tools needed for a more responsible action. However, schools are dominated by a traditional work, which does not allow for enough and proper attention to these relevant and current questions, and which involve a critical analysis of the conceptions and values established by our civilization, resulting in the present patterns of science-technology-society relations and their influence on the environment and health. Several research works have indicated a diversity of barriers that obstruct this necessary change. Since formal education plays an important role in the education of society for this theme, in this paper the possibilities and difficulty dealing with this problem are discussed, focusing on the question of teacher education, based on investigations related to teacher education programs for environmental education. The data collected show the urgency for introducing this theme in teacher training programs, taking into account the fact that educators have their own conceptions, values and attitudes that should be considered in these programs.

Effects of monocrotaline on energy metabolism in the rat liver

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Histoenzymological and ultrastructural changes in lateral muscle fibers of Oreochromis niloticus (Teleostei: Cichlidae) after local injection of veratrine

The effects of veratrine have been investigated in mammalian, amphibian, and crustacean muscle, but not in fish. In this work, the action of veratrine was studied in the lateral muscle of the freshwater teleost Oreochromis niloticus after intramuscular injection. Histoenzymological typing and electron microscopy of muscle fibers before and 15, 30, and 60 min after veratrine injection (10 ng/kg fish) were used to indirectly assess the morphological changes and the oxidative and m-ATPase activities. In some cases, muscles were pretreated with tetrodotoxin to determine whether the ultrastructural changes were the result of Na+ channel activation by veratrine. Veratrine altered the metabolism of fibers mainly after 30 min. Oxidative fibers showed decreased NADH-TR activity, whereas that of glycolytic and oxidative-glycolytic type fibers increased. There was no change in the m-ATPase activity of the three fiber types, except at 60 min postveratrine, when a novel fiber type, which showed no reversal after acidic and alkaline preincubations, appeared. Ultrastructural damage involved sarcomeres, myofibrils, and mitochondria, but the T-tubules remained intact. Pretreatment with tetrodotoxin (1 ng/ml) prevented the ultrastructural changes caused by veratrine. These results show that in fish skeletal muscle veratrine produces some effects that are not seen in mammalian muscle.

Enzymes present in the thoracic gland extracts from workers and males of Apis mellifera (Hymenoptera: Apidae)

This research deals with the analysis of the enzymes present in thoracic gland extracts from newly emerged, nurse workers, forager workers, newly emerged males, and mature males of A. mellifera L. (Hymenoptera, Apoidea, Apidae). The enzymes found in larger quantities in the thoracic gland occurred in all classes of workers and are digestive. Acid phosphatase and Naphtol-AS-BI-phosphohydrolase act in protein synthesis, leucine arylamidase hydrolyses proteins and a-glucosidase actuate in the nectar processing into honey. Naphtol-AS-BI-phosphohydrolase was found in larger quantities only in workers, this suggests action in protein synthesis by the thoracic gland, b-galactosidase is in larger amounts in the newly emerged bees (workers and males) this aids in the provision of other substances to be used as an energy source when glucose or sucrose are absent. Differences between enzymatic profiles from workers and males are usually related to their colony tasks, or related to their physiological necessities per individual in specific life stages.

Calling sites and acoustic partitioning in species of the Hyla nana and rubicundula groups (Anura, Hylidae)

We analysed spatial and acoustic partitioning among four species of Hyla belonging to two species-groups: nana (H. nana and H. sanborni) and rubicundula (H. elianeae and H. jimi). Field activities were conducted at three permanent ponds, from 1998 through 2001. Four attributes of the calling sites were analysed: perch height, distance of the perch from the edge of the pond, type of perch (vegetation) and the individual's position on the perch. There was extensive overlap in the four calling-site variables analysed. However, we found spatial segregation did occur in calling site height and the distance of perches from pond edges. Bioacoustic analyses revealed behavioural differences among species in calling activity, both time of onset and peak calling in chorus. There was acoustic partitioning among species the fundamental frequency of the advertisement calls, principally as a function of the temporal structure (e.g. note duration, rate of note repetition, duration and rate of repetition of the calling pulses). We propose that differences in physical attributes of calling site and in characteristics of calls allow these species to exist in sympatry.

Investigation of photo-bleaching through transmittance method in pigmented solution: Understanding possible mechanisms and advantages for photo dental whitening

The dental bleaching is known for many years. Recently a technique employing light has open up new and exciting possibilities. Besides its vast application there are still many important points to be understood about teeth photon bleaching. In this work we present an in vitro experiment to explore the main mechanisms involved during the photon action in tooth whitening. Our results indicated that light at same wavelengths are great absorbed by pigments creating a local heating which considerably increase the bleaching rate. This results in a fast reaction without heating the whole dental structure. We discuss details of our experiment. Work supported by Fapesp and CNPq.

False alarms in damage detection approaches

Structural Health Monitoring (SHM) denotes a system with the ability to detect and interpret adverse changes in structures in order to improve reliability and reduce life-cycle costs. The greatest challenge for designing a SHM system is knowing what changes to look for and how to classify them. Different approaches for SHM have been proposed for damage identification, each one with advantages and drawbacks. This paper presents a methodology for improvement in vibration signal analysis using statistics information involving the probability density. Generally, the presence of noises in input and output signals results in false alarms, then, it is important that the methodology can minimize this problem. In this paper, the proposed approach is experimentally tested in a flexible plate using a piezoelectric (PZT) actuator to provide the disturbance.

A territorial perspective: towards the consolidation of human settlements in Latin America and the Caribbean

Includes bibliography

The Role of Tax Policy in the Context of the Global Crisis: consequences and prospects

Includes bibliography

Development and validation of a Xanthomonas axonopodis pv. citri DNA microarray platform (XACarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome

Background. From shotgun libraries used for the genomic sequencing of the phytopathogenic bacterium Xanthomonas axonopodis pv. citri (XAC), clones that were representative of the largest possible number of coding sequences (CDSs) were selected to create a DNA microarray platform on glass slides (XACarray). The creation of the XACarray allowed for the establishment of a tool that is capable of providing data for the analysis of global genome expression in this organism. Findings. The inserts from the selected clones were amplified by PCR with the universal oligonucleotide primers M13R and M13F. The obtained products were purified and fixed in duplicate on glass slides specific for use in DNA microarrays. The number of spots on the microarray totaled 6,144 and included 768 positive controls and 624 negative controls per slide. Validation of the platform was performed through hybridization of total DNA probes from XAC labeled with different fluorophores, Cy3 and Cy5. In this validation assay, 86% of all PCR products fixed on the glass slides were confirmed to present a hybridization signal greater than twice the standard deviation of the deviation of the global median signal-to-noise ration. Conclusions. Our validation of the XACArray platform using DNA-DNA hybridization revealed that it can be used to evaluate the expression of 2,365 individual CDSs from all major functional categories, which corresponds to 52.7% of the annotated CDSs of the XAC genome. As a proof of concept, we used this platform in a previously work to verify the absence of genomic regions that could not be detected by sequencing in related strains of Xanthomonas. © 2010 Moreira et al; licensee BioMed Central Ltd.

Economic efficiency of free and open source software in the public sector: the example of Chile

Includes bibliography

Poverty, hunger and food security in Central America and Panama

Includes bibliography

Limitations of trust management schemes in VANET and countermeasures

Vehicular networks ensure that the information received from any vehicle is promptly and correctly propagated to nearby vehicles, to prevent accidents. A crucial point is how to trust the information transmitted, when the neighboring vehicles are rapidly changing and moving in and out of range. Current trust management schemes for vehicular networks establish trust by voting on the decision received by several nodes, which might not be required for practical scenarios. It might just be enough to check the validity of incoming information. Due to the ephemeral nature of vehicular networks, reputation schemes for mobile ad hoc networks (MANETs) cannot be applied to vehicular ad hoc networks (VANET). We point out several limitations of trust management schemes for VANET. In particular, we identify the problem of information cascading and oversampling, which commonly arise in social networks. Oversampling is a situation in which a node observing two or more nodes, takes into consideration both their opinions equally without knowing that they might have influenced each other in decision making. We show that simple voting for decision making, leads to oversampling and gives incorrect results. We propose an algorithm to overcome this problem in VANET. This is the first paper which discusses the concept of cascading effect and oversampling effects to ad hoc networks. © 2011 IEEE.