Influencia de cultivos antecesores y métodos de control de malezas sobre la cenosis de las malezas y el crecimiento, desarrollo y rendimiento de los cultivos de maíz (Zea mays L.) y sorgo (Sorghum bicolor (L.) Moench)

En este trabajo se estudió la influencia de diferentes rotaciones de cultivos y métodos de control de malezas, sobre la dinámica de la cenósis de malezas y el crecimiento, desarrollo y rendimiento de los cultivos Maíz y Sorgo. El ensayo se inició en primera de 1992, en los meses de mayo a agosto, en la cooperativa Rubén Duarte, Managua. Se utilizó un diseño de parcelas divididas arregladas en bloque completo al azar. Siendo el factor A: Ajonjolí -Maíz, Soya-Maíz, Ajonjolí-Sorgo, Soya-Sorgo y el factor B: control químico, control período crítico, control limpia periódica. Los resultados demuestran que el control limpia periódica efectuó un control satisfactorio de malezas, mientras los controles periodo crítico y químico fueron insuficientes, debido a la predominancia de Cyperus rotundus y poáceas como Cenchrus sp y Rottboellia cochinchinensis. Las diferentes rotaciones influyeron sobre el nivel de enmalezamiento, siendo más bajo en la rotación Ajonjolí-Sorgo y Soya-Sorgo que en las restantes rotaciones. En cuanto a rendimiento, los mejores resultados se obtuvieron en las rotaciones Soya-Maíz y Ajonjolí-Sorgo, en éstas, los cultivos antecesores ejercieron un aceptable control de malezas.

Evaluación del rendimiento de veinticinco líneas de sorgo (Sorghum bicolor L. Moench) bajo dos niveles de fertilización en el municipio de San Isidro-Matagalpa (2004)

El estudio se realizó en la Finca el Nancite ubicada en la comarca El Bocón del municipio de San Isidro _ Matagalpa, en la época de postrera (agosto _ diciembre) del año 2004. Elobjetivo de esta investigación fue evaluar veinticinco líneas de sorgo (Sorghum bicolor L. Moench), bajo dos niveles de fertilización (37.43 kg N ha-1 y 0 kg N ha-1). Los materiales evaluados son líneas puras y pertenecen al programa ICRISAT/LASIP; ICSVLM, que significa en ingles Sorghum Variety Latín American Program. El diseño que se utilizó para el experimento fue un diseño de bloques completos al azar (BCA). Cada una de las repeticiones estaba constituida por veinticinco parcelas en donde se sembraron las líneas a evaluar. Cada parcela contenía seis surcos de los cuales tres se fertilizaron aplicándosele completo (10-30-10) al momento de la siembra y fertilizante nitrogenado urea al 46% aplicado en forma fraccionada un 50% a los 30 (dds) y el otro 50% a los 45 días después de la siembra (dds), los otros tres surco no recibieron aporte de fertilización química sintética de forma aplicada. Las variables a evaluar en el desarrollo del cultivo fueron: Altura de planta (cm), diámetro del tallo (mm) y número de hojas por planta, estas se evaluaron en tres momentos (45, 60, 75dds). Al momento de la cosecha se evaluaron: Longitud de la panoja (cm.), longitud del raquis (cm), biomasa seca producida (kg ha-1), porcentaje de nitrógeno en la biomasa (%), porcentaje de nitrógeno en el grano (%), rendimiento de grano (kg ha-1), acumulación de nitrógeno en el grano (kg ha-1 ), acumulación de nitrógeno en la biomasa (kg ha-1), eficiencia fisiológica (%), incremento del rendimiento de grano por kg de nitrógeno aplicado (%), eficiencia de uso de nitrógeno por el grano (%). Los resultados obtenidos de las variables evaluadas se procesaron en el paquete estadístico FUALNM (versión 0.5) y separación de medias por Tukey al 95% de confiabilidad (α= 0.05), los resultados obtenidos de acuerdo al análisis, para las variables de desarrollo (altura, numero de hojas y diámetro), para largo de panoja y largo de raquis fueron altamente significativo para ambos factores (A: Líneas, B: Fertilización), en los diferentes momentos evaluados, exceptuando para la variable numero de hojas a los 75 dds para el factor B que fue significativo y para el diámetro del tallo a los 60 dds el factor B que no hubo significancia, todas estas variables mencionadas mostraron diferencias mínimas numéricamente. Para la biomasa seca producida y rendimiento de grano mostraron ser altamente significativo para los dos factoresA y B, obteniendo las líneas ICSVLM-89527, ICSVLM-89551 promedios de 5664 y 4912 kg.ha-1, siendo estos los mayores rendimientos de biomasa de todas las líneas. El rendimiento del grano, los mayores valores lo obtuvieron las líneas ICSVLM-89527 y ICSVLM-89551 con 1526.5 y 1271.8 kg. ha-1. En el incremento de rendimiento por kg. de N aplicado las líneas con mayores resultados fueron la ICSVLM-90538 con 14.7 % y las ICSVLM-93079 con 12.8%. Las líneas que hicieron un mejor uso eficiente de nitrógeno por la biomasa y el grano fueron la JOCORO y el testigo PINOLERO.

La edición crítica de Amadís de Gaula de Garci Rodríguez de Montalvo

Integran este número de la revista ponencias presentadas en Studia Hispanica Medievalia VIII: Actas de las IX Jornadas Internacionales de Literatura Española Medieval, 2008, y de Homenaje al Quinto Centenario de Amadis de Gaula

Garci Sánchez de Badajoz y la propulsión del Cancionero General

Integran este número de la revista ponencias presentadas en Studia Hispanica Medievalia VIII : Actas de las X Jornadas Internacionales de Literatura Española Medieval, 2011, y de Homenaje al Quinto Centenario del Cancionero General de Hernando del Castillo.

Activation of violaxanthin cycle in darkness is a common response to different abiotic stresses : a case study in Pelvetia canaliculata

Background: In the violaxanthin (V) cycle, V is de-epoxidized to zeaxanthin (Z) when strong light or light combined with other stressors lead to an overexcitation of photosystems. However, plants can also suffer stress in darkness and recent reports have shown that dehydration triggers V-de-epoxidation in the absence of light. In this study, we used the highly stress-tolerant brown alga Pelvetia canaliculata as a model organism, due to its lack of lutein and its non-photochemical quenching independent of the transthylakoidal-ΔpH, to study the triggering of the V-cycle in darkness induced by abiotic stressors. Results: We have shown that besides desiccation, other factors such as immersion, anoxia and high temperature also induced V-de-epoxidation in darkness. This process was reversible once the treatments had ceased (with the exception of heat, which caused lethal damage). Irrespective of the stressor applied, the resulting de-epoxidised xanthophylls correlated with a decrease in Fv/Fm, suggesting a common function in the down-regulation of photosynthetical efficiency. The implication of the redox-state of the plastoquinone-pool and of the differential activity of V-cycle enzymes on V-de-epoxidation in darkness was also examined. Current results suggest that both violaxanthin de-epoxidase (VDE) and zeaxanthin-epoxidase (ZE) have a basal constitutive activity even in darkness, being ZE inhibited under stress. This inhibition leads to Z accumulation. Conclusion: This study demonstrates that V-cycle activity is triggered by several abiotic stressors even when they occur in an absolute absence of light, leading to a decrease in Fv/Fm. This finding provides new insights into an understanding of the regulation mechanism of the V-cycle and of its ecophysiological roles.

Evaluation of bioactive sphingolipids in 4-HPR-resistant leukemia cells

Background -- N-(4-hydroxyphenyl)retinamide (4-HPR, fenretinide) is a synthetic retinoid with potent pro-apoptotic activity against several types of cancer, but little is known regarding mechanisms leading to chemoresistance. Ceramide and, more recently, other sphingolipid species (e.g., dihydroceramide and dihydrosphingosine) have been implicated in 4-HPR-mediated tumor cell death. Because sphingolipid metabolism has been reported to be altered in drug-resistant tumor cells, we studied the implication of sphingolipids in acquired resistance to 4-HPR based on an acute lymphoblastic leukemia model. Methods -- CCRF-CEM cell lines resistant to 4-HPR were obtained by gradual selection. Endogenous sphingolipid profiles and in situ enzymatic activities were determined by LC/MS, and resistance to 4-HPR or to alternative treatments was measured using the XTT viability assay and annexin V-FITC/propidium iodide labeling. Results -- No major crossresistance was observed against other antitumoral compounds (i.e. paclitaxel, cisplatin, doxorubicin hydrochloride) or agents (i.e. ultra violet C, hydrogen peroxide) also described as sphingolipid modulators. CCRF-CEM cell lines resistant to 4-HPR exhibited a distinctive endogenous sphingolipid profile that correlated with inhibition of dihydroceramide desaturase. Cells maintained acquired resistance to 4-HPR after the removal of 4-HPR though the sphingolipid profile returned to control levels. On the other hand, combined treatment with sphingosine kinase inhibitors (unnatural (dihydro)sphingosines ((dh)Sph)) and glucosylceramide synthase inhibitor (PPMP) in the presence or absence of 4-HPR increased cellular (dh)Sph (but not ceramide) levels and were highly toxic for both parental and resistant cells. Conclusions -- In the leukemia model, acquired resistance to 4-HPR is selective and persists in the absence of sphingolipid profile alteration. Therapeutically, the data demonstrate that alternative sphingolipid-modulating antitumoral strategies are suitable for both 4-HPR-resistant and sensitive leukemia cells. Thus, whereas sphingolipids may not be critical for maintaining resistance to 4-HPR, manipulation of cytotoxic sphingolipids should be considered a viable approach for overcoming resistance.

Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord

Background: The adult central nervous system (CNS) contains different populations of immature cells that could possibly be used to repair brain and spinal cord lesions. The diversity and the properties of these cells in the human adult CNS remain to be fully explored. We previously isolated Nestin(+) Sox2(+) neural multipotential cells from the adult human spinal cord using the neurosphere method (i.e. non adherent conditions and defined medium). -- Results: Here we report the isolation and long term propagation of another population of Nestin(+) cells from this tissue using adherent culture conditions and serum. QPCR and immunofluorescence indicated that these cells had mesenchymal features as evidenced by the expression of Snai2 and Twist1 and lack of expression of neural markers such as Sox2, Olig2 or GFAP. Indeed, these cells expressed markers typical of smooth muscle vascular cells such as Calponin, Caldesmone and Acta2 (Smooth muscle actin). These cells could not differentiate into chondrocytes, adipocytes, neuronal and glial cells, however they readily mineralized when placed in osteogenic conditions. Further characterization allowed us to identify the Nkx6.1 transcription factor as a marker for these cells. Nkx6.1 was expressed in vivo by CNS vascular muscular cells located in the parenchyma and the meninges. -- Conclusion: Smooth muscle cells expressing Nestin and Nkx6.1 is the main cell population derived from culturing human spinal cord cells in adherent conditions with serum. Mineralization of these cells in vitro could represent a valuable model for studying calcifications of CNS vessels which are observed in pathological situations or as part of the normal aging. In addition, long term propagation of these cells will allow the study of their interaction with other CNS cells and their implication in scar formation during spinal cord injury.

A short in-frame deletion in NTRK1 tyrosine kinase domain caused by a novel splice site mutation in a patient with congenital insensitivity to pain with anhidrosis

Background: Congenital insensitivity to pain with anhidrosis (CIPA) is a rare autosomal recessive genetic disease characterized by the lack of reaction to noxious stimuli and anhidrosis. It is caused by mutations in the NTRK1 gene, which encodes the high affinity tyrosine kinase receptor I for Neurotrophic Growth Factor (NGF). -- Case Presentation: We present the case of a female patient diagnosed with CIPA at the age of 8 months. The patient is currently 6 years old and her psychomotor development conforms to her age (RMN, SPECT and psychological study are in the range of normality). PCR amplification of DNA, followed by direct sequencing, was used to investigate the presence of NTRK1 gene mutations. Reverse transcriptase (RT)-PCR amplification of RNA, followed by cloning and sequencing of isolated RT-PCR products was used to characterize the effect of the mutations on NTRK1 mRNA splicing. The clinical diagnosis of CIPA was confirmed by the detection of two splice-site mutations in NTRK1, revealing that the patient was a compound heterozygote at this gene. One of these alterations, c.574+1G > A, is located at the splice donor site of intron 5. We also found a second mutation, c.2206-2 A > G, not previously reported in the literature, which is located at the splice acceptor site of intron 16. Each parent was confirmed to be a carrier for one of the mutations by DNA sequencing analysis. It has been proposed that the c.574+1G > A mutation would cause exon 5 skipping during NTRK1 mRNA splicing. We could confirm this prediction and, more importantly, we provide evidence that the novel c.2206-2A > G mutation also disrupts normal NTRK1 splicing, leading to the use of an alternative splice acceptor site within exon 17. As a consequence, this mutation would result in the production of a mutant NTRK1 protein with a seven aminoacid in-frame deletion in its tyrosine kinase domain. --Conclusions: We present the first description of a CIPA-associated NTRK1 mutation causing a short interstitial deletion in the tyrosine kinase domain of the receptor. The possible phenotypical implications of this mutation are discussed.

Depletion of the heaviest stable N isotope is associated with NH4 +/NH3 toxicity in NH4 +-fed plants

Background: In plants, nitrate (NO(3)(-)) nutrition gives rise to a natural N isotopic signature (delta(15)N), which correlates with the delta(15)N of the N source. However, little is known about the relationship between the delta(15)N of the N source and the (14)N/(15)N fractionation in plants under ammonium (NH(4)(+)) nutrition. When NH(4)(+) is the major N source, the two forms, NH(4)(+) and NH(3), are present in the nutrient solution. There is a 1.025 thermodynamic isotope effect between NH(3) (g) and NH(4)(+)(aq) which drives to a different delta(15)N. Nine plant species with different NH(4)(+)-sensitivities were cultured hydroponically with NO(3)(-) or NH(4)(+) as the sole N sources, and plant growth and delta(15)N were determined. Short-term NH(4)(+)/NH(3) uptake experiments at pH 6.0 and 9.0 (which favours NH(3) form) were carried out in order to support and substantiate our hypothesis. N source fractionation throughout the whole plant was interpreted on the basis of the relative transport of NH(4)(+) and NH(3). -- Results: Several NO(3)(-)-fed plants were consistently enriched in (15)N, whereas plants under NH(4)(+) nutrition were depleted of (15)N. It was shown that more sensitive plants to NH(4)(+) toxicity were the most depleted in (15)N. In parallel, N-deficient pea and spinach plants fed with (15)NH(4)(+) showed an increased level of NH(3) uptake at alkaline pH that was related to the (15)N depletion of the plant. Tolerant to NH(4)(+) pea plants or sensitive spinach plants showed similar trend on (15)N depletion while slight differences in the time kinetics were observed during the initial stages. The use of RbNO(3) as control discarded that the differences observed arise from pH detrimental effects. -- Conclusions: This article proposes that the negative values of delta(15)N in NH(4)(+)-fed plants are originated from NH(3) uptake by plants. Moreover, this depletion of the heavier N isotope is proportional to the NH(4)(+)/NH(3) toxicity in plants species. Therefore, we hypothesise that the low affinity transport system for NH(4)(+) may have two components: one that transports N in the molecular form and is associated with fractionation and another that transports N in the ionic form and is not associated with fractionation.

Identification of a biomarker panel for colorectal cancer diagnosis

Background: Malignancies arising in the large bowel cause the second largest number of deaths from cancer in the Western World. Despite progresses made during the last decades, colorectal cancer remains one of the most frequent and deadly neoplasias in the western countries. Methods: A genomic study of human colorectal cancer has been carried out on a total of 31 tumoral samples, corresponding to different stages of the disease, and 33 non-tumoral samples. The study was carried out by hybridisation of the tumour samples against a reference pool of non-tumoral samples using Agilent Human 1A 60- mer oligo microarrays. The results obtained were validated by qRT-PCR. In the subsequent bioinformatics analysis, gene networks by means of Bayesian classifiers, variable selection and bootstrap resampling were built. The consensus among all the induced models produced a hierarchy of dependences and, thus, of variables. Results: After an exhaustive process of pre-processing to ensure data quality–lost values imputation, probes quality, data smoothing and intraclass variability filtering–the final dataset comprised a total of 8, 104 probes. Next, a supervised classification approach and data analysis was carried out to obtain the most relevant genes. Two of them are directly involved in cancer progression and in particular in colorectal cancer. Finally, a supervised classifier was induced to classify new unseen samples. Conclusions: We have developed a tentative model for the diagnosis of colorectal cancer based on a biomarker panel. Our results indicate that the gene profile described herein can discriminate between non-cancerous and cancerous samples with 94.45% accuracy using different supervised classifiers (AUC values in the range of 0.997 and 0.955).

Panama Canal Twenty-fifth anniversary 1914 - Aug. 15 -1939

Leonard Carpenter Panama Canal Collection. Photographs: Views of Panama and the Canal. [Box 1] from the Special Collections & Area Studies Department, George A. Smathers Libraries, University of Florida. Booklet dedication: Published under the direction of a committee appointed by Brigadier General Clarence S. Ridley, Governor of The Panama Canal, to arrange suitable ceremonies, as authorized in Public Resolution No.5, 76th Congress, approved March 28, 1939, to celebrate the twenty-fifth anniversary of the opening of the Panama Canal to commerce. Prepared by Rufus Hardy, Executive Department, The Panama Canal. (120 page document)

Canal diggers in Panama, 1904 to 1928

Leonard Carpenter Panama Canal Collection. Publication: The Canal Diggers in Panama 1904 to 1928 [Box 1] from the Special Collections & Area Studies Department, George A. Smathers Libraries, University of Florida. (10 page document)

Coastal engineering investigation at Jupiter Inlet, Florida

A fixed-bed hydraulic model of Jupiter Inlet, Florida, was constructed for the purpose of testing measures designed to remedy problems of sediment erosion and deposition in the inlet area. Both tide-induced flows as well as waves were simulated in the model which was built on an undistorted scale of 1:49. Model verification was based on prototype measurements of waves, tides and currents. Results have been interpreted in terms of the influence of various proposed remedial schemes on flow velocity magnitude, distribution and wave height at various locations within the study area. A stability parameter has been utilized for evaluating the degree of sediment erosion or deposition at a given location. Various structural solutions were examined in the model. It is proposed that, in the initial phase of solution implementation, sediment removal/nourishment methods be used primarily to mitigate the existing problems. New structures, as per model test results, should be installed under subsequent phases, only if sediment management procedures do not prove to be adequate. The currently followed procedure of periodic sand trap dredging may be extended to include the new dredging/nourishment requirements. (PDF contains 245 pages.)

The artesian water of the Ruskin area of Hillsborough County, Florida: interim report

The purpose of the investigation is to make a detailed study of the geology and ground water in the Ruskin area, especially as related to the problem of salt-water encroachment. The major objectives of the program includes: (1) An inventory of wells to determine their number and distribution, their depths and diameters, and other pertinent information. (2) A study of artesian pressures. (3) Analyses of water from selected wells to determine the location and extent of any areas in which the artesian water is salty. (4) A study of the surface and subsurface geology as related to the occurrence and movement of ground water. (5) An estimate of the quantity of ground water withdrawn. (PDF contains 24 pages.)