Structural and ultrastructural evidence for telocytes in prostate stroma

The prostate comprises a glandular epithelium embedded within a fibromuscular stroma. The stroma is a complex arrangement of cells and extracellular matrix (ECM) components in addition to growth factors, regulatory molecules, remodelling enzymes, blood vessels, nerves and immune cells. The principal sources of ECM components are fibroblasts and smooth muscle cells (SMC), which synthesize the structural and regulatory components of the ECM. Telocytes (TCs) were recently described as a novel stromal cell type that exhibited characteristic features. The aim of this study was to confirm the presence of TCs in prostate stromal tissue of gerbils, as the stromal compartment of this gland is a dynamic microenvironment. We used transmission electron microscopy (TEM), light microscopy and immunohistochemistry methods to provide morphological evidence for the presence of TCs. Cells that resembled TCs were observed in gerbil prostatic stroma. These cells had small cellular bodies with very thin and extremely long cellular processes. They were found primarily in the subepithelial area and also at the periphery of SMC layers. TCs also exhibited moniliform processes, caveolae and nuclei surrounded by small amounts of cytoplasm. Close contacts between TC podomers were evident, particularly in the adjacent epithelial compartment. This morphological evidence supported the presence of TCs in the gerbil prostatic stroma, which we report for the first time. © 2013 The Authors. Published by Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

An Integrative Genomic and Transcriptomic Analysis Reveals Potential Targets Associated with Cell Proliferation in Uterine Leiomyomas

Background: Uterine Leiomyomas (ULs) are the most common benign tumours affecting women of reproductive age. ULs represent a major problem in public health, as they are the main indication for hysterectomy. Approximately 40-50% of ULs have non-random cytogenetic abnormalities, and half of ULs may have copy number alterations (CNAs). Gene expression microarrays studies have demonstrated that cell proliferation genes act in response to growth factors and steroids. However, only a few genes mapping to CNAs regions were found to be associated with ULs. Methodology: We applied an integrative analysis using genomic and transcriptomic data to identify the pathways and molecular markers associated with ULs. Fifty-one fresh frozen specimens were evaluated by array CGH (JISTIC) and gene expression microarrays (SAM). The CONEXIC algorithm was applied to integrate the data. Principal Findings: The integrated analysis identified the top 30 significant genes (P<0.01), which comprised genes associated with cancer, whereas the protein-protein interaction analysis indicated a strong association between FANCA and BRCA1. Functional in silico analysis revealed target molecules for drugs involved in cell proliferation, including FGFR1 and IGFBP5. Transcriptional and protein analyses showed that FGFR1 (P = 0.006 and P<0.01, respectively) and IGFBP5 (P = 0.0002 and P = 0.006, respectively) were up-regulated in the tumours when compared with the adjacent normal myometrium. Conclusions: The integrative genomic and transcriptomic approach indicated that FGFR1 and IGFBP5 amplification, as well as the consequent up-regulation of the protein products, plays an important role in the aetiology of ULs and thus provides data for potential drug therapies development to target genes associated with cellular proliferation in ULs. © 2013 Cirilo et al.

Qualidade estrutural de um latossolo vermelho submetido à compactação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Oxidação da calcopirita (CuFe S'IND.2') por Acidithiobacillus ferooxidans em presença de cisteína e de Acidithiobacillus thiooxidans

Pós-graduação em Biotecnologia - IQ

O sentido da autoridade nas destituições do poder familiar em Marília a partir dos relatos dos operadores do direito na perspectiva do gênero

Pós-graduação em Ciências Sociais - FFC

Utilização do controle estatístico de processo na unidade de produção e desenvolvimento de derivados de soja - UNISOJA - F.C.F. - UNESP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização edafoclimática e proposta para a determinação do risco de redução de safra na região do Pontal do Paranapanema - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Coação administrativa: limites e responsabilidades

Pós-graduação em Direito - FCHS

The development of services in the Caribbean

Includes bibliography

Identificação de assinaturas de seleção e anotação do genoma em bovinos da raça Nelore

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Characteristics of households with ICTs in Latin America and the Caribbean

Includes bibliography

Diversidade de geofungos em águas do Parque Municipal do Ibirapuera na cidade de São Paulo, SP, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo do turismo na perspectiva geográfica no município de Presidente Epitácio

Pós-graduação em Geografia - IGCE

Farmacocinética pré-clínica e cardiotoxicidade da doxorrubicina veiculada por sistema microemulsionado

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência da alimentação inicial no crescimento muscular e na expressão da MyoD e Miogenina em larvas de pacu

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)