967 resultados para HOMOGENEOUS POLYNOMIALS


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Supported by: Contract AT (11-1)-1018 with the U.S. Atomic Energy Commission and the Advanced Research Projects Agency.

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"C00-1469-0154."

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Bibliography: p. 24.

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"(This is being submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Mathematics, June 1959.)"

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"Other issues of this report may bear the number BAW-7."

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Photocopy of microfilm. Springfield, Va. : National Technical Information Service, [1979]

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Mode of access: Internet.

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"From Proceedings of the American Academy of Arts and Sciences, v.38, no. 9, Oct. 1902."

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Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent surfaces, in suspension, or on scaffolds, have a number of drawbacks, including the need for manual selection to achieve a homogeneous population and the use of nonphysiological matrix compounds. In this study we describe a mild method for the generation of MCTS, in which individual spheroids form in hanging drops suspended from a microtiter plate. The method has been successfully applied to a broad range of cell lines and shows nearly 100% efficiency (i.e., one spheroid per drop). Using the hepatoma cell line, HepG2, the hanging drop method generated well-rounded MCTS with a narrow size distribution (coefficient of variation [CV] 10% to 15%, compared with 40% to 60% for growth on nonadherent surfaces). Structural analysis of HepG2 and a mammary gland adenocarcinoma cell line, MCF-7, composed spheroids, revealed highly organized, three-dimensional, tissue-like structures with an extensive extracellular matrix. The hanging drop method represents an attractive alternative for MCTS production, because it is mild, can be applied to a wide variety of cell lines, and can produce spheroids of a homogeneous size without the need for sieving or manual selection. The method has applications for basic studies of physiology and metabolism, tumor biology, toxicology, cellular organization, and the development of bioartificial tissue. (C) 2003 Wiley Periodicals, Inc.

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We give a detailed exposition of the theory of decompositions of linearised polynomials, using a well-known connection with skew-polynomial rings with zero derivative. It is known that there is a one-to-one correspondence between decompositions of linearised polynomials and sub-linearised polynomials. This correspondence leads to a formula for the number of indecomposable sub-linearised polynomials of given degree over a finite field. We also show how to extend existing factorisation algorithms over skew-polynomial rings to decompose sub-linearised polynomials without asymptotic cost.