La ruta a la Mar del Sur y la fundación de Ibarra, siglos XVII-XVIII (Estudios)

El estudio analiza el intento de las autoridades coloniales quiteñas de poner en marcha un proyecto vial que conecte la región centro-norte de la Sierra con la costa del Pacífico en el siglo XVII. Con este propósito, se fundó la villa de San Miguel de Ibarra, un centro urbano de enlace que permitiría concretar dicho proyecto viaL El artículo enfatiza en la ubicación estratégica de la ciudad, la participación del Cabildo, las aspiraciones económicas de las élites regionales, la participación de Pedro Vicente Maldonado y las dificultades y oposiciones que impidieron la realización del proyecto.

Los Mitos de la Feminidad: su reflejo en "Las Vendas" de Raúl Pérez Torres, "Tren Nocturno" de Abdón Ubidia, "El Hombre de la Mirada Oblicua" de Javier Vásconez, "El Apátrida" de Vladimiro Rivas lturralde y "Recordando el Mar" de Francisco Proaño Arandi

Los años 70 y 80 fueron una época de renovación y revaluación de la forma y el fondo de la literatura ecuatoriana. Me pareció significativo que en una época de búsqueda de identidad y de vanguardia en la literatura la mujer haya sido representada por los escritores como un ser plano y unidimensional. cubierta por una gruesa capa de silencio. A pesar de que existen numerosos estudios sobre el período en cuestión y sobre los autores y textos escogidos ("Las Vendas" de Raúl Pérez Torres, "Tren Nocturno" de Abdón Ubidia. "El Hombre de la Mirada Oblicua" de Javier Vásconez. "El Apátrida" de Vladimiro Rivas Iturralde y "Recordm1do el Mar de Francisco Proaño Arandi), ninguno ponía especial énfasis en el rol que desempeñan las mujeres dentro de esos relatos. Me pareció una tarea descuidada y necesaria. Ya en 1975 Michael H. Handelsman señalaba en Amazonas y Artistas que faltan estudios sobre "la imagen de la mujer en las obras de los escritores". Me pareció que la teoría feminista era la más adecuada para llevar adelante este análisis pues ésta busca demostrar que la literatura y el lenguaje tienen relevancia en la creación de la sociedad y considera que el género es una construcción social. Dentro del análisis he puesto especial énfasis en el (a) rol del lector en ese constante flujo de información que se forma entre el texto, el autor y el lector: y (b) la importancia de la ideología en la construcción del sujeto y la contribución de los aparatos ideológicos del estado en la formación de éste.

Ecuador y los temas del nuevo Derecho del Mar

Este trabajo tiene como objetivo presentar al lector una investigación sobre la Convención de las Naciones Unidas sobre el De recho del Mar (CONVEMAR) y, en especial, sobre la frontera marítima que comparte el Ecuador con sus tres vecinos. El texto presenta un análisis cronológico y cualitativo sobre la evolución del Derecho del Mar, su aplicación y límites, teniendo como referentes a la Tercera Conferencia de las Naciones Unidas sobre el Derecho del Mar, de 1982, cuando se cristaliza su consolidación en un cuerpo normativo claro, y la Convención de las Naciones sobre el Derecho del Mar (CONVEMAR o UNCLOS, en inglés), de 1994, cuando se inicia su vigencia y utilización. También aborda la situación de las islas Galápagos y la incidencia en temas jurisdiccionales, ambientales y de reconocimiento de la membresía del Ecuador en la CONVEMAR. Otro de los pilares de la investigación propuesto en este análisis es la delimitación de la frontera marítima, específicamente con el Perú, vecino país del Sur del Ecuador. Para esto se analizan cuáles son los discursos y visiones de los países en cuestión, realidades cartográficas, aceptaciones tácitas y acciones de reconocimiento de la frontera delimitada por la latitud 3°23”33,96” S.

Hombres de mar. Caciques de la Costa ecuatoriana en los inicios de la época colonial (Estudios)

This article explains how the colonial economic system was set up on the what is now the Ecuadorian coast. It also shows how social and precolonial economic forms funcioned and its articulation with the colonial economy. In this geographic zone where merchandise was exchanged and where people mingled and information was shared linking the distant mother country with the viceroyalty, the production and circulation of a wide array of goods provided the cornerstone for crestfallen indigenas societies. The sources provide evidence on the methods used by local rulers, who were essential actors in colonial implantation.

Persistence of a wild type Escherichia coli and its multiple antibiotic-resistant (MAR) derivatives in the abattoir and on chilled pig carcasses

The aim of this study was to evaluate the ability of an Escherichia coli with the multiple antibiotic resistance (MAR) phenotype to withstand the stresses of slaughter compared to an isogenic progenitor strain. A wild type E. coli isolate (345-2RifC) of porcine origin was used to derive 3 isogenic MAR mutants. Escherichia coli 345-2RifC and its MAR derivatives were inoculated into separate groups of pigs. Once colonisation was established, the pigs were slaughtered and persistence of the E. coli strains in the abattoir environment and on the pig carcasses was monitored and compared. No significant difference (P>0.05) was detected between the shedding of the different E. coli strains from the live pigs. Both the parent strain and its MAR derivatives persisted in the abattoir environment, however the parent strain was recovered from 6 of the 13 locations sampled while the MAR derivatives were recovered from 11 of 13 and the number of MAR E. coil recovered was 10-fold higher than the parent strain at half of the locations. The parent strain was not recovered from any of the 6 chilled carcasses whereas the MAR derivatives were recovered from 3 out of 5 (P<0.001). This study demonstrates that the expression of MAR in 345-2RifC increased its ability to survive the stresses of the slaughter and chilling processes. Therefore in E. coli, MAR can give a selective advantage, compared to non-MAR strains, for persistence on chilled carcasses thereby facilitating transit of these strains through the food chain. (C) 2010 Elsevier B.V. All rights reserved.

Fitness and dissemination of disinfectant-selected multiple-antibiotic-resistant (MAR) strains of Salmonella enterica serovar Typhimurium in chickens

Objectives: The aims of this study were to determine whether strains of Salmonella enterica serovar Typhimurium which had acquired low-level multiple antibiotic resistance (MAR) through repeated exposure to farm disinfectants were able to colonize and transmit between chicks as easily as the parent strain and, if such strains were less susceptible to fluoroquinolones, would high-level resistance be selected after fluoroquinolone treatment. Methods: Two mutants were compared with the isogenic parent. In the first experiment, day-old chicks were co-infected with both the parent and a mutant to determine their relative fitness. In the second experiment, parent and mutant strains (in separate groups of chicks) were assessed for their ability to transmit from infected (contact) to non-infected (naive) birds and with respect to their susceptibility to fluoroquinolone treatment. Birds were regularly monitored for the presence of Salmonella in caecal contents. Replica plating was used to monitor for the selection of antibiotic-resistant strains. Results: The parent strain was shown to be significantly fitter than the two mutants and was more rapidly disseminated to naive birds. Antibiotic treatment did not preferentially select for the two mutants or for resistant strains. Conclusions: The disinfectant-exposed strains, although MAR, were less fit, less able to disseminate than the parent strain and were not preferentially selected by therapeutic antibiotic treatment. As such, these strains are unlikely to present a greater problem than other salmonellae in chickens.

Role of the mar locus in virulence of Salmonella enterica serovar Typhimurium DT104 in chickens

The virulence of a Salmonella enterica serovar Typhimurium DT014 strain in which marA was insertionally inactivated was compared to its isogenic parent in vitro and in vivo. In vitro, the numbers of the marA mutant phagocytosed by porcine lung macrophages were significantly increased, while survival at 24 h inside macrophages and adherence to human gut cells were significantly reduced in comparison with the parent strain. In vivo, the marA inactivated strain, in competition with its parent strain, persisted for a shorter period in chickens, was present in the caeca at significantly lower levels and invaded the deeper organs to a significantly lesser extent. Therapeutic antibiotic treatment of one group of chickens with oxytetracycline favoured the persistence of both the parent strain and, to a lesser extent, the marA inactivated strain; but interestingly, increased tetracycline resistance of Salmonella isolates after treatment of birds with antibiotic was seen only for the parent strain. Further work is needed to elucidate how mar is involved in virulence and if its inactivation can minimise the ability of bacteria to become antibiotic-resistant in vivo.

Multiple antibiotic resistance (mar) locus in Salmonella enterica serovar typhimurium DT104

In order to understand the role of the mar locus in Salmonella with regard to multiple antibiotic resistance, cyclohexane resistance, and outer membrane protein F (OmpF) regulation, a marA::gfp reporter mutant was constructed in an antibiotic-sensitive salmonella enterica serovar Typhimurium DT104 background. Salicylate induced marA, whereas a number of antibiotics, disinfect ants, and various growth conditions did not. Increased antibiotic resistance was observed upon salicylate induction, although this was shown to be by both mar-dependent and mar-independent pathways. Cyclohexane resistance, however, was induced by salicylate by a mar-dependent pathway. Complementation studies with a plasmid that constitutively expressed marA confirmed the involvement of map in Salmonella with low-level antibiotic resistance and cyclohexane resistance, although the involvement of mar in down regulation of OmpF was unclear. However, marA overexpression did increase the expression of a ca. 50-kDa protein, but its identity remains to be elucidated Passage of the marA::gfp reporter mutant with increasing levels of tetracycline, a method reported to select for mar mutants in Escherichia coli, led to both multiple-antibiotic and cyclohexane resistance. Collectively, these data indicate that low-level antibiotic resistance, cyclohexane resistance, and modulation of OMPs in Salmonella, as in E. coli, can occur in both a mar-dependent and mar-independent manner.

The multiple antibiotic resistance (mar) locus and its significance

Chromosomally encoded systems involved in low level resistance of bacteria to different classes of antibiotics (mainly beta-lactams, chloramphenicol, quinolones and tetracycline), disinfectants and in resistance to organic solvents have been the focus of considerable interest in recent years. The multiple antibiotic resistance (mar) locus of Escherichia coli and Salmonella is perhaps the best described system involved in this type of resistance which is induced by MarA, the activator protein encoded by the marRAB locus. The mar-locus is reported to mediate resistance primarily by up-regulating efflux of some antibiotics, disinfectants and organic solvents via the AcrAB-TolC efflux pump and down regulating influx through Outer Membrane Protein F (OmpF). Whilst the level of antibiotic resistance conferred by marRAB is only low level, there are increasing data to suggest that marRAB and related systems are important in clinical antibiotic resistance, possibly as a 'stepping stone' to higher levels of resistance. Other related systems include up-regulation of RobA, SoxS and AcrAB which give rise to a similar resistance phenotype to that conferred by up-regulation of MarA. The aim of this paper is to review the function and significance of the mar-locus and related systems with a particular focus on its implications in veterinary medicine. (C) 2002 Published by Elsevier Science Ltd.

Anthropogenic nutrient sources and loads from a Mediterranean catchment into a coastal lagoon: Mar Menor, Spain

The Mar Menor is a coastal lagoon increasingly threatened by urban and agricultural pressures. The main watercourse draining into the lagoon is the Rambla del Albujón. A fortnightly campaign carried out over one annual cycle enabled us to characterize the treated urban sewage effluents and agricultural sources which contribute to the nutrient fluxes in the watercourse. Multivariate analysis provided information for establishing chemical signatures and for assessing the relative influence of the various sources on the water quality at the outlet. Mass balances were used to examine net gains and losses, and cross-correlations with rainfall to analyze climatic influence and control factors in the trends of the nutrient flux. The rainfall pattern was significantly cross-correlated with nitrate and phosphorus fluxes from agricultural sources, while fluctuations in the resident population explained the phosphorus flux trend in urban sources. 50% of dissolved inorganic nitrogen was from agricultural sources, while 70% of total phosphate and 91% of total organic carbon were from urban point sources. The net amounts of all the nutrients fell as a result of plant uptake and/or denitrification in the channel. The control of urban point sources (phosphorus-enriched) is suggested as a promptly action for improving the health of the coastal lagoon.

Denudation history of the Bocaina Plateau, Serra do Mar, southeastern Brazil: Relationships to Gondwana breakup and passive margin development

The Bocaina Plateau, which is situated on the eastern flank of the continental rift of southeastern Brazil, is the highest part of the Serra do Mar. Topographic relief in this area is suggested to be closely related to its complex tectono-magmatic evolution since the breakup of Western Gondwana and opening of the South Atlantic Ocean. Apatite fission track ages and track length distributions from 27 basement outcrops were determined to assess these hypotheses and reconstruct the denudation history of the Bocaina Plateau. The ages range between 303 +/- 32 and 46 +/- 5 Ma, and are significantly younger than the stratigraphic ages. Mean track lengths vary from 13.44 +/- 1.51 to 11.1 +/- 1.48 mu m, with standard deviations between 1.16 and 1.83 mu m. Contrasting ages within a single plateau and similar ages at different altitudes indicate a complex regional tectonothermal evolution. The thermal histories inferred from these data imply three periods of accelerated cooling related to the Early Cretaceous continental breakup, Early Cretaceous alkaline magmatism, and the Paleogene evolution of the continental rift of southeastern Brazil. The oldest fission track ages (>200 Ma) were obtained in the Serra do Mar region, suggesting that these areas were a long-lived source of sediments for the Parana, Bauru, and Santos basins. (C) 2010 International Association for Gondwana Research. Published by Elsevier B.V. All rights reserved.

"Porque quiero casarme con un varón hermoso de la orilla del mar" Un análisis de lo grotesco femenino de María Josefa en La casa de Bernarda Alba

This essay is an analysis of the character María Josefa in the play 'The House of Bernarda Alba' (1936) by Federico García Lorca. It is hypothesized that the character of María Josefa can be considered a distorting mirror of the femininity presented in the play, and that through María Josefa this femininity is both revealed and problematized. The analysis adopts theories from the field of the (female) grotesque, using terminology both from Mikhail Bakhtin and Mary Russo. Throughout the analysis it is demonstrated how the character of María Josefa challenges the boundaries and norms which are dictated by Bernarda to control the women of the house. These conventions are challenged by María Josefa with the use of her loud speech, her dressed up appearance, and in her physical resistance. In this manner María Josefa is creating and embodying an alternate feminine view that is uncovered through her consistent provocation and by making herself into "a spectacle", a transboundary behaviour that is well interpreted within the theories of carnival, the grotesque body, and the spectacle of the female grotesque.

Influência da temperatura e da presença de H2S no comportamento mecânico e eletroquímico do aço API 5CT grau P110 em água do mar sintética

o presente trabalho teve por objetivo estudar o comportamento de um aço de alta resistência e baixa liga (com amostras de composição aproximada de 0,4% C, 0,6% Cr e 0,4% Mo), da classe API scr PIlO, utilizado na perfuração de petróleo offshore, frente a processos de fragilização causados pelo meio. Água do mar sintética foi utilizada como meio, com intuito de padronizar, em laboratório, as condições a que o material fica submetido na prática. Buscou-se avaliar e comparar o comportamento mecânico do material pela modificação dos parâmetros: temperatura, potencial aplicado ao material, e o efeito da presença ou ausência de H2S na solução. Para isso, foram realizados ensaios de tração pelo método de baixa taxa de deformação (da ordem de 10-6S-I), obtendo-se as curvas tensão x deformação nas diferentes situações ensaiadas, comparadas com as obtidas em óleo mineral. Análises fratográficas também foram utilizadas como forma de caracterizar os processos de fragilização. Além disso, estudou-se o comportamento eletroquímico do material nas diferentes condições através de ensaios de polarização potenciostáticos. Com isso, pode-se determinar quais as condições mais danosas e de maior risco para a utilização do material e em quais delas o seu uso é seguro. O aço estudado apresentou-se susceptível a processos de fragilização e todos os parâmetros analisados mostraram-se importantes no estudo desses processos.