Distribuição e conservação dos genes que codificam as proteínas VgrG e Hcp em espécies de Aeromonas

Aeromonas spp. são bastonetes Gram negativos amplamente distribuídos nos ambientes aquáticos, com relatos de isolamento em água de abastecimento público e alimentos. Este micro-organismo possui potencial de causar doenças intestinais e extraintestinais cuja patogenicidade está associada a sua virulência multifatorial. Diversos determinantes de virulência de Aeromonas já foram identificados, incluindo sistemas de secreção de proteínas. O sistema de secreção tipo VI (SST6) é o mais recente sistema de secreção de proteínas identificado em bactérias cuja presença em estirpes no gênero Aeromonas pode implicar atividades de citotoxicidade para o hospedeiro, pois esse sistema é capaz de injetar moléculas efetoras dentro da célula, interferindo diretamente nos processos celulares. A fim de determinar a presença e analisar a distribuição dos genes hcp e vgrG codificadores das proteínas efetoras do SST6 em Aeromonas spp. o presente estudo examinou 119 cepas isoladas de diversas origens pela técnica da PCR após o desenho de oligonucleotídeos iniciadores específicos. Objetivamos ainda analisar a variabilidade genética interespecífica dos genes hcp e vgrG a partir de dados de sequenciamento. Os resultados obtidos indicaram a distribuição dos genes vgrG e hcp em 46% das cepas de Aeromonas hydrophila e Aeromonas caviae de diferentes origens. Entre as cepas de A. hydrophila a maior frequência foi observada nas cepas isoladas de humanos, onde todas foram positivas para os iniciadores que amplificaram um produto de 541 pb do gene vgrG e 418 pb do gene hcp. Entre as cepas de A. caviae, a incidência de genes vgrG e hcp foi mais elevada nas cepas isoladas de alface (60%) e peixes (50%). As cepas analisadas de origem ambiental apresentaram índice total de 36% de positividade, apresentando frequência de 60% e 22% em A. hydrophila e A. caviae, respectivamente. Os dados obtidos da análise de cepas de origem alimentar mostraram a presença dos genes vgrG e hcp em 67% (A. hydrophila) e 60% (A. caviae) das cepas isoladas de folhas de alface. Nas cepas isoladas de queijo os genes foram encontrados em 67% e 12,5% das cepas de A. hydrophila e de A. caviae, respectivamente. O alinhamento múltiplo entre as sequências dos segmentos dos genes hcp e vgrG obtidas no sequenciamento indicou grau de identidade nucleotídica de 75 a 100% entre as sequências de hcp e 80 a 100% entre as sequências de vgrG. Em conclusão, nossos resultados indicaram que os iniciadores desenhados foram capazes de detectar suas sequências alvo em cepas de A. caviae e outras espécies de Aeromonas, sugerindo a existência de homologia entre os genes nas diferentes espécies, confirmada após sequenciamento de DNA. Os dados indicaram que esses genes estão distribuídos em várias espécies de Aeromonas e em cepas isoladas de diversas fontes. Ressaltamos a prevalência de cepas de A. hydrophila PCR-positivas em isolados clínicos, sugerindo a participação do SST6 no complexo universo da virulência multifatorial que permeia esse micro-organismo

La datación de la gráfica rupestre de apariencia paleolítica: un siglo de conjeturas y datos

[ES] Hace un siglo Altamira y La Mouthe suscitaron el primer discurso de autentificación de la gráfica rupestre de apariencia paleolítica. Se repasan varios casos recientes de peritaje del arte rupestre occidental que recurren a conjeturas, datos y argumentos varios para validar grabados y pinturas «descontextualizados» (al margen de depósitos estratificados y que no pueden ser datados directamente). En estos años 90 nuevas perspectivas de datación y análisis intentan precisar (¿renovar?) los argumentos de autentificación.

Autenticacão de Assinaturas Online: Estudo dos Parâmetros do Dynamic Time Warping e da Representação da Assinatura

O reconhecimento de padões é uma área da inteligência computacional que apoia a resolução de problemas utilizando ferramentas computacionais. Dentre esses problemas podem ser citados o reconhecimento de faces, a identificação de impressões digitais e a autenticação de assinaturas. A autenticação de assinaturas de forma automática tem sua relevância pois está ligada ao reconhecimento de indivíduos e suas credenciais em sistemas complexos e a questões financeiras. Neste trabalho é apresentado um estudo dos parâmetros do Dynamic Time Warping, um algoritmo utilizado para alinhar duas assinaturas e medir a similaridade existente entre elas. Variando-se os principais parâmetros desse algoritmo, sobre uma faixa ampla de valores, foram obtidas as médias dos resultados de erros na classificação, e assim, estas médias foram avaliadas. Com base nas primeiras avaliação, foi identificada a necessidade de se calcular um desses parâmetros de forma dinâmica, o gap cost, a fim de ajustá-lo no uso de uma aplicação prática. Uma proposta para a realização deste cálculo é apresentada e também avaliada. É também proposta e avaliada uma maneira alternativa de representação dos atributos da assinatura, de forma a considerar sua curvatura em cada ponto adquirido no processo de aquisição, utilizando os vetores normais como forma de representação. As avaliações realizadas durante as diversas etapas do estudo consideraram o Equal Error Rate (EER) como indicação de qualidade e as técnicas propostas foram comparadas com técnicas já estabelecidas, obtendo uma média percentual de EER de 3,47%.

PCR-SSCP and Isoelectric Focusing as Screening Methods for Identification of (Sub-) Tropical Fish Species

To ensure the authentication of fishery products lacking biological characters, rapid species identification methods are required. Two DNA- and protein-based methods, PCR-SSCP (polymerase chain reaction - single strand conformation polymorphism) of a 464 bp segment of the cytochrome b – gene and isoelectric focusing (IEF) of water-soluble proteins from fish fillets, were applied to identify fillets of (sub-) tropical fish species available on the European market. Among the samples analysed were two taxonomically identified species from the family Sciaenidae and one from Sphyraenidae. By comparison of DNA- and protein patterns of different samples, information about intra-species variability of patterns, and homogeneity of batches (e.g. fillet blocks or bags) can be obtained. PCR-SSCP and IEF may be useful for pre-checking of a large number of samples by food control laboratories. Zusammenfassung Zur Sicherstellung der Authentizität von Fischerei-Erzeugnissen ohne biologische Merkmale sind schnelle Verfahren zur Speziesidentifizierung hilfreich. Zwei Methoden der DNA- bzw. Protein-Analyse wurden eingesetzt, um Filets (sub-) tropischer Fischarten, die auf dem europäischen Markt angeboten werden, zu identifizieren. Bei diesen Methoden handelt es sich um die PCR-SSCP (Polymerase-Kettenreaktion – Einzelstrang-Konformationspolymorphismus) – Analyse der PCR-Produkte und die IEF (isoelektrische Fokussierung) der wasserlöslichen Fischmuskelproteine. Unter den untersuchten Proben waren zwei taxonomisch bestimmte Arten aus der Familie Sciaenidae und eine Spezies aus der Familie Sphyraenidae. Durch Vergleich der DNA- bzw. Proteinmuster lassen sich Informationen über die intra-spezifische Variabilität solcher Muster und die Einheitlichkeit von Partien (beispielsweise Filetblöcke oder Filetbeutel) gewinnen. PCR-SSCP und IEF können in Laboratorien der Lebensmittelüberwachung als Vortest gerade bei hohen Probenzahlen sinnvoll eingesetzt werden.

Incremental Linear Discriminant Analysis Using Sufficient Spanning Sets and Its Applications

This paper presents an incremental learning solution for Linear Discriminant Analysis (LDA) and its applications to object recognition problems. We apply the sufficient spanning set approximation in three steps i.e. update for the total scatter matrix, between-class scatter matrix and the projected data matrix, which leads an online solution which closely agrees with the batch solution in accuracy while significantly reducing the computational complexity. The algorithm yields an efficient solution to incremental LDA even when the number of classes as well as the set size is large. The incremental LDA method has been also shown useful for semi-supervised online learning. Label propagation is done by integrating the incremental LDA into an EM framework. The method has been demonstrated in the task of merging large datasets which were collected during MPEG standardization for face image retrieval, face authentication using the BANCA dataset, and object categorisation using the Caltech101 dataset. © 2010 Springer Science+Business Media, LLC.

Isolating and recognizing crudeoiklegrading [sic] bacteria of Queshm Island shores for biodegradation of crude oil pollutions

This research was carried out for recognizing Natural Flora Bacteria of oil pollution in the coasts of Queshm island. In The First steps, The coasts of this Island were scrutinized as a Field of research and For knowing whether oil stains exist or not. It gets obvious That southern coasts of Queshm have got oil pollution which is created by oil tankers which carry oil of Iran continental shelf. Them oil stains were sampled from to certain stations. In The First step, primary isolation of exisiting bacteria in every oil sample was done and then purification of each bacterium was carried out. Then each purified bacterium that has got strong, recognized, typic growth was enriched oil sample of T5 station. And Bacterium C4 (gram—negative coccobacillus) was chosen as the second priority From oil sample of TA station and Bacterium B1 (gram—positive coccus) was chosen as The third priority From oil sample of TI station. All The above mentioned bacteria were biochemically, physiologically and morphologically experimented For specking The species. According To The tests done and comparing with The tests done and comparing with the reference Berge y' s, bacterium A5 Pelongs to the species pseudomonas sp and becterium C4 belongs to the species Aeromonas sp and bacterium BI belongs to The species micrococcus sp. In The Last stage, bacterium with The First priority (TA5 pseudomonas sp) was used in the planned microcosm. The sake of optimum and adapting to Laboratory conditions Each enriched and purified bacterium was given a code for station and a code For itself . Then This bacterium was studied and it was proved that it has potentiality For using oil as a source of carbon. From oil samples of 10 stations, 30 various Colonies of bacterium were Isolated, of which 20 bacteria had the highest potentiality of growth. And the other bacteria that has no typic growth were omitted From being studied. Since all of These 20 bacterium are able to use oil, a bacterium with maximum rate of growth in the presence of crude oil and Lack of other hydrocarbonic sources and with The code A5 ( gram — negative Bacillus ) was chosen as First priority From The mentioned microcosm contains sea water , suspension oil degrading bacterium , crude oil, azote and various concentrations of carbon and Incubated in 30°` and shook 150 PRA1 According to the results , index oil degrading bacterium (pseudomonas sp) belongs oil sample of T5 stations (east of sheeb draz Gulf) which growth best and have the potentiality of degrading oil in 25 glli malas and 50 glli cheese water and with 5 gill urea .

Polymer film packaging for food: An environmental assessment

Plastics packaging is ubiquitous in the food industry, fulfilling a range of functions including a significant role in reducing food waste. The public perception of packaging, however, is dominated by end-of-life aspects, when the packaging becomes waste often found littering urban, rural and marine environments. A balanced analysis of the role of packaging demands that the whole lifecycle is examined, looking not only at the packaging itself but also at the product being packaged. This paper focuses on packaging in the meat and cheese industry, analysing the impact of films and bags. The functions of packaging are defined and the environmental impact of delivering these functions is assessed. The influence of packaging on levels of waste and energy consumption elsewhere in the system is examined, including the contentious issue of end-of-life for packaging. Strategies for minimizing the environmental impact of the packaging itself involve reduction in the amount of material used (thinner packaging), rather than emphasizing end-of-life issues. Currently, with polymer recycling not at a high level, evidence suggests that this strategy is justifiable. Biodegradable polymers may have some potential for improving environmental performance, but are still problematic. The conclusion is that although current packaging is in some ways wasteful and inefficient, the alternatives are even less desirable. © 2013 Elsevier B.V. All rights reserved.

Expressive RFID data access policies for the Pharmaceuticals supply chain

The Pharma(ceuticals) industry is at a cross-roads. There are growing concerns that illegitimate products are penetrating the supply chain. There are proposals in many countries to apply RFID and other traceability technologies to solve this problem. However there are several trade-offs and one of the most crucial is between data visibility and confidentiality. In this paper, we use the TrakChain assessment framework tools to study the US Pharma supply chain and to compare candidate solutions to achieve traceability data security: Point-of-Dispense Authentication, Network-based electronic Pedigree, and Document-based electronic Pedigree. We also propose extensions to a supply chain authorization language that is able to capture expressive data sharing conditions considered necessary by the industry's trading partners. © 2013 IEEE.

Improving the safety and security of the pharmaceutical supply chain

This paper discusses various techniques that may be used to combat counterfeiting in the pharmaceutical supply chain. These include the use of electronic pedigrees (to ensure the integrity of the supply chain), together with mass-serialization (to provide for a unique lifecycle history of each individual package) and authentication of the product (to check for any discrepancies in the various attributes of the product and its packaging are as intended for that individual package). Management of the pedigree process and product authentication is discussed in some detail, together with various other learnings from the Drug Security Network, including identification of some remaining vulnerabilities and suggestions for tightening these loopholes. © 2008 Springer-Verlag Berlin Heidelberg.

RSA-Based Password-Authenticated Key Exchange, Revisited

The RSA-based Password-Authenticated Key Exchange (PAKE) protocols have been proposed to realize both mutual authentication and generation of secure session keys where a client is sharing his/her password only with a server and the latter should generate its RSA public/private key pair (e, n), (d, n) every time due to the lack of PKI (Public-Key Infrastructures). One of the ways to avoid a special kind of off-line (so called e-residue) attacks in the RSA-based PAKE protocols is to deploy a challenge/response method by which a client verifies the relative primality of e and φ(n) interactively with a server. However, this kind of RSA-based PAKE protocols did not give any proof of the underlying challenge/response method and therefore could not specify the exact complexity of their protocols since there exists another security parameter, needed in the challenge/response method. In this paper, we first present an RSA-based PAKE (RSA-PAKE) protocol that can deploy two different challenge/response methods (denoted by Challenge/Response Method1 and Challenge/Response Method2). The main contributions of this work include: (1) Based on the number theory, we prove that the Challenge/Response Method1 and the Challenge/Response Method2 are secure against e-residue attacks for any odd prime e; (2) With the security parameter for the on-line attacks, we show that the RSA-PAKE protocol is provably secure in the random oracle model where all of the off-line attacks are not more efficient than on-line dictionary attacks; and (3) By considering the Hamming weight of e and its complexity in the RSA-PAKE protocol, we search for primes to be recommended for a practical use. We also compare the RSA-PAKE protocol with the previous ones mainly in terms of computation and communication complexities.

匿名凭证与匿名口令认证技术研究

随着隐私保护越来越为人们所关注，对匿名认证技术的研究已经成为学术领域的一个热点。本文主要着眼于匿名认证中的匿名凭证与匿名口令认证密钥协商。 在对匿名凭证技术的研究中，本文重点分析了防止凭证出借与凭证匿名更新两个匿名凭证系统性质的实现。 凭证出借是指匿名凭证系统中，用户可以随意将自己的凭证与他人共享，从而使多人可以同时使用一个凭证。本文提出一种新的防止凭证出借方法，并给出一个具体的实现方案。该方法将凭证出借与用户隐私联系起来，通过凭证本身实现防止凭证出借。凭证匿名更新则是指凭证内容进行更新时，凭证颁发方只能知道变更的内容信息，不知道其他任何信息。本文提出一种新的实现方法，在原有凭证的基础上，用相对较少的计算量来实现对凭证内容的更新，使用户匿名得到一个新的凭证。 在对匿名口令认证密钥协商的研究中，本文首先提出了两个攻击方案：针对Shin等人的TAP(t≥2)协议的内部假扮攻击和针对TAP(t≥2)协议以及Viet等人的k-out-of-n APAKE协议的离线字典攻击。前者破坏了协议的认证性，内部攻击者可以假扮服务器与用户建立会话密钥。后者破坏了协议最基本的对口令的安全保护，使得内部攻击者可以离线猜测组内所有用户口令。然后，本文提出了一个新的两方的匿名口令认证密钥协商协议：NAPAKE，并在Square Computational Diffiee-Hellman困难假设以及Decision Inverted-Additive Diffie-Hellman困难假设下证明其安全性。同时，还进一步将其扩展为D-NAPAKE协议，以实现多方的匿名口令认证密钥协商，该协议可以抵抗上面的两个攻击。

一次身份认证可访问多个应用服务器

一个企业网中的应用服务多种多样，各自都提供了安全措施，这必然给用户使用和管理员的权限管理带来不便。为此，设计和开发了一套一次身份认证系统。该系统可与各个应用服务很好地集成在一起，在保证安全的前提下方便了用户的使用和权限的管理。

基于椭圆曲线的零知识证明方法对Kerberos系统的改进

Kerberos是一个成熟的产品,广泛应用于金融、邮电、保险等行业.但仍存在一些隐患,例如:重放攻击、密码猜测、会话中选择明文攻击等等.该文针对Kerberos系统登录时可能遭到密码猜测,即所谓的离线字典攻击(Off line Dictionary Attack)的问题,提出一种基于椭圆曲线的零知识证明方法对系统进行改进,并给出相应的协议.

关于“为设计认证协议的一个简明逻辑”一文的注记

Buttyan等人提出了一个简洁的逻辑 ,他们把它用于改进 Woo- L am协议 ,并且未证明地声称 :改进后的协议是抗协议与自身的交互攻击的 .为表明他们的结论是不正确的 ,找到了改进协议的两个不同的攻击 ,并详细解释如何加以实现 .构造攻击的方式除了要求更细致之外 ,与 Debbabi等人的方式在本质上是相似的 .进一步的分析表明Debbabi等人的逻辑没有足够的能力推理交互攻击 ,该逻辑有待改进

基于模糊提取的远程双向生物认证

传统的远程生物认证采用安全信道或者生物认证过程本地化的方法,具有较多的局限性.模糊提取可从生物特征输入中以容错的方式可靠地提取出均匀分布的随机密钥,当输入发生变化且变化很小时,该密钥保持不变.基于这一重要工具,给出了一个零存储的非安全信道双向生物认证方案.该方案无需存储和传输用户的生物特征,有效保护了用户隐私,并能够抵抗假冒攻击和多服务器合谋攻击.此外,所给方案还具有良好的可扩展性,集成口令和智能卡可产生多因素认证方案,并支持用户注册更新.