Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection

On a reversed phase Hypersil BDS C-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11H-benzo [a] carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. Optimum derivatization was obtained by reacting of amino acids with BCEC-Cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. The fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. The identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. Linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids.

Determination of amines using 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC-Cl) as labeling reagent by HPLC with fluorescence detection and identification with APCI/MS

A pre-column derivatization method for the sensitive determination of amines using a labeling reagent 2-(11H-benzo[a]-carbazol-11-yl) ethyl chloroformate (BCEC-Cl) followed by high-performance, liquid chromatography with fluorescence detection has been developed. Identification of derivatives was carried out by LC/APCI/MS in positive-ion mode. The chromophore of 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC-Cl) reagent was replaced by 2-(11H-benzo[a]-carbazol-11-yl) ethyl functional group, which resulted in a sensitive fluorescence derivatizing reagent BCEC-Cl. BCEC-Cl could easily and quickly label amines. Derivatives were stable enough to be efficiently analyzed by HPLC and showed an intense protonated molecular ion corresponding m/z [M+ H](+) under APCI/MS in positive-ion mode. The collision-induced dissociation of the protonated molecular ion formed characteristic fragment ions at m/z 261.8 and m/z 243.8 corresponding to the cleavages of CH2O-CO and CH2-OCO bonds. Studies on derivatization demonstrated excellent derivative yields over the pH 9.0-10.0. Maximal yields close to 100% were observed with three- to four-fold molar reagent excess. In addition, the detection responses for BCEC-derivatives were compared to those obtained using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC-Cl) and 9-fluorenyl methylchloroformate, (FMOC-Cl) as labeling reagents. The ratios I-BCEC/I-BCEOC = 1.94-2.17 and I-BCEC/I-FMOC = 1.04-2.19 for fluorescent (FL) responses (here, I was relative fluorescence intensity). Separation of the derivatized amines had been optimized on reversed-phase Eclipse XDB-C-8 column. Detection limits calculated from 0.50 pmol injection, at a signal-to-noise ratio of 3, were 1.77-14.4 fmol. The relative standard deviations for within-day determination (n = 11) were 1.84-2.89% for the tested amines. The mean intra- and inter-assay precision for all amines levels were < 3.64% and 2.52%, respectively. The mean recoveries ranged from 96.6% to 107.1% with their standard deviations in the range of 0.8-2.7. Excellent linear responses were observed with coefficients of > 0.9996. (C) 2006 Elsevier B.V. All rights reserved.

珠江口盆地流花11-1油田储层非均质性及流体分布规律研究

In order to discover the distribution law of the remaining oil, the paper focuses on the quantitative characterization of the reservoir heterogeneity and the distribution law of the fluid barrier and interbed, based on fine geological study of the reservoir in Liuhuall-1 oil field. The refined quantitative reservoir geological model has been established by means of the study of core analysis, logging evaluation on vertical well and parallel well, and seismic interpretation and prediction. Utilizing a comprehensive technology combining dynamic data with static data, the distribution characteristics, formation condition and controlling factors of remaining oil in Liuhuall-1 oil field have been illustrated. The study plays an important role in the enrichment regions of the remaining oil and gives scientific direction for the next development of the remaining oil. Several achievements have been obtained as follows: l.On the basis of the study of reservoir division and correlation,eight lithohorizons (layer A, B_1, B_2, B_3, C, D, E, and F) from the top to the bottom of the reservoir are discriminated. The reef facies is subdivided into reef-core facies, fore-reef facies and backreef facies. These three subfacies are further subdivided into five microfacies: coral algal limestone, coralgal micrite, coral algal clastic limestone, bioclastic limestone and foraminiferal limestone. In order to illustrate the distribution law of remaining oil in high watercut period, the stratigraphic structure model and sedimentary model are reconstructed. 2.1n order to research intra-layer, inter-layer and plane reservoir heterogeneity, a new method to characterize reservoir heterogeneity by using IRH (Index of Reservoir Heterogeneity) is introduced. The result indicates that reservoir heterogeneity is medium in layer B_1 and B_3, hard in layer A, B_2, C, E, poor in layer D. 3.Based on the study of the distribution law of fluid barrier and interbed, the effect of fluid battier and interbed on fluid seepage is revealed. Fluid barrier and interbed is abundant in layer A, which control the distribution of crude oil in reservoir. Fluid barrier and interbed is abundant relatively in layer B_2,C and E, which control the spill movement of the bottom water. Layer B_1, B_3 and D tend to be waterflooded due to fluid barrier and interbed is poor. 4.Based on the analysis of reservoir heterogeneity, fluid barrier and interbed and the distribution of bottom water, four contributing regions are discovered. The main lies on the north of well LH11-1A. Two minors lie on the east of well LH11-1-3 and between well LH11-1-3 and well LH11-1-5. The last one lies in layer E in which the interbed is discontinuous. 5.The parameters of reservoir and fluid are obtained recurring to core analysis, logging evaluation on vertical well and parallel well, and seismic interpretation and prediction. Theses parameters provide data for the quantitative characterization of the reservoir heterogeneity and the distribution law of the fluid barrier and interbed. 6.1n the paper, an integrated method about the distribution prediction of remaining oil is put forward on basis of refined reservoir geological model and reservoir numerical simulation. The precision in history match and prediction of remaining oil is improved greatly. The integrated study embodies latest trend in this research field. 7.It is shown that the enrichment of the remaining oil with high watercut in Liuhua 11-1 oil field is influenced by reservoir heterogeneity, fluid barrier and interbed, sealing property of fault, driving manner of bottom water and exploitation manner of parallel well. 8.Using microfacies, IRH, reservoir structure, effective thickness, physical property of reservoir, distribution of fluid barrier and interbed, the analysis of oil and water movement and production data, twelve new sidetracked holes are proposed and demonstrated. The result is favorable to instruct oil field development and have gotten a good effect.

7-11岁正常儿童和ADHD儿童故事重述能力及策略的发展

儿童的故事重述涉及工作记忆和抽象表征能力，是与儿童的日常学业任务密切联系。儿童能否把握时间线索来组织故事重述，反映的是儿童抽象出故事事件内在时间关系的能力，因此对儿童故事重述的探查可以揭示儿童抽象思维发展的一般规律和特点。本研究在前人研究的基础上，系统探查了7－11岁儿童故事重述能力及其策略的发展过程，探讨了时间线索对儿童故事重述的影响，并对ADHD儿童和正常儿童的故事重述及策略进行了比较。 主要研究结果如下： (1)7－11岁儿童故事重述能力总体上随年龄增长而提高，按正确顺序重述的事件数量增多，重述过程中提取和使用的时间线索也增加。其中7－9岁有较快速的发展。 (2)随故事中包含的事件数量的增加，故事难度增大，儿童的故事重述成绩有所下降，故事长度对7岁和9岁儿童的影响大于对11岁儿童的影响。 (3)有、无时间线索语词会影响儿童的故事重述。故事中包含时间语词线索有助于儿童的故事重述。11岁儿童的故事重述已不受有、无时间线索的影响，他们在无时间线索的条件下，更多地主动使用表示因果关系的语词来表征事件关系。 (4)ADHD儿童的故事重述能力在9岁到11岁有较快的发展。但总体而言，ADHD儿童对含有时间线索故事的重述能力显著差于正常儿童。 (5)故事再学习对ADHD儿童和正常儿童的故事重述都存在促进作用。但ADHD儿童故事再学习效果不如正常儿童，低龄儿童中差距尤其明显，随着年龄的增加，ADHD儿童与正常儿童故事再学习效果的差距逐渐缩小。 (6)不同年龄的儿童主要采用四种策略解决问题。随年龄增长，正常儿童和ADHD儿童中使用水平较高的内容主线和逻辑关系策略的人数都有所增加。但ADHD儿童中高水平策略使用人数比例仍低于正常儿童。

Message-Driven Processor Architecture: Verson 11

The Message-Driven Processor is a node of a large-scale multiprocessor being developed by the Concurrent VLSI Architecture Group. It is intended to support fine-grained, message passing, parallel computation. It contains several novel architectural features, such as a low-latency network interface, extensive type-checking hardware, and on-chip memory that can be used as an associative lookup table. This document is a programmer's guide to the MDP. It describes the processor's register architecture, instruction set, and the data types supported by the processor. It also details the MDP's message sending and exception handling facilities.

Avaliação do desempenho econômico-financeiro da produção de soja no Estado do Paraná, para a safra 2010/11.

2010

Avaliação do desempenho econômico-financeiro da produção de soja nos estados do Rio Grande do Sul e de Santa Catarina, para a safra 2010/11.

Procedimentos para a estimativa de custos e lucros na produção de soja. Procedimentos para a estimativa do custo de oportunidade na produção de soja. Resultados. Estimativa do custo de produção da soja transgênica, por hectare, na região que abrange o município de Passo Fundo, RS, safra 2010/11. Estimativa do custo de produção da soja transgênica, por hectare, na região que abrange o município de Ibirubá, RS, safra 2010/11. Estimativa do custo de produção da soja convencional, por hectare, na região que abrange o município de Campos Novos, SC, safra 2010/11. Estimativa do custo de produção da soja transgênica, por hectare, na região que abrange o município de Campos Novos, SC, safra 2010/11. Síntese econômica da produção da soja transgênica, por hectare, na região que abrange o município de Passo Fundo, RS, safra 2010/11. Síntese econômica da produção da soja transgênica, por hectare, na região que abrange o município de Ibirubá, RS, safra 2010/11. Síntese econômica da produção da soja convencional, por hectare, na região que abrange o município de Campos Novos, SC, safra 2010/11. Síntese econômica da produção da soja transgênica, por hectare, na região que abrange o município de Campos Novos, SC, safra 2010/11.