Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm

Glycerol and dimethyl sulfoxide (DMSO) are widely used as penetrating cryoprotectants in the freezing of sperm, and various concentrations are applied in different species and laboratories. The present study aimed to examine the effect of these two cryoprotectants at different concentrations (2%, 5%, 10%, and 15% glycerol or DMSO) on rhesus monkey sperm cryopreservation. The results showed that the highest recovery of post-thaw sperm motility, and plasma membrane and acrosome integrity was achieved when the sperm was frozen with 5% glycerol. Spermatozoa cryopreserved with 15% DMSO showed the lowest post-thaw sperm motility, and spermatozoa cryopreserved with 15% glycerol and 15% DMSO showed the lowest plasma membrane integrity among the eight groups. The results achieved with 5% glycerol were significantly better for all parameters than those obtained with 5% DMSO. The functional cryosurvival of sperm frozen with 5% glycerol was further assessed by in vitro fertilization (IVF). Overall, 85.7% of the oocytes were successfully fertilized, and 51.4% and 5.7% of the resulting zygotes developed into morulae and blastocysts, respectively. The results indicate that the type and concentration of the penetrating cryoprotectant used can greatly affect the survival of rhesus monkey sperm after it is frozen and thawed. The suitable glycerol level for rhesus monkey sperm freezing is 5%, and DMSO is not suitable for rhesus monkey sperm cryopreservation. (C) 2004 Wiley-Liss, Inc.

The effects of corona treatment on impact and spreading of ink-jet drops on a polymeric film substrate

The effects of varying corona surface treatment on ink drop impact and spreading on a polymer substrate have been investigated. The surface energy of substrates treated with different levels of corona was determined from static contact angle measurement by the Owens and Wendt method. A drop-on-demand print-head was used to eject 38 μm diameter drops of UV-curable graphics ink travelling at 2.7 m/s on to a flat polymer substrate. The kinematic impact phase was imaged with a high speed camera at 500k frames per second, while the spreading phase was imaged at 20k frames per secoiui. The resultant images were analyzed to track the changes in the drop diameter during the different phases of drop spreading. Further experiments were carried out with white-light intetferometry to accurately measure the final diameter of drops which had been printed on different corona treated substrates and UV cured. The results are correlated to characterize the effects of corona treatment on drop impact behavior and final print quality.

无指盘臭蛙丝氨酸蛋白酶抑制剂及其基因和应用

本发明涉及一种无指盘臭蛙丝氨酸蛋白酶抑制剂及其基因和在制药中的应用，属于生物医学领域。无指盘臭蛙丝氨酸蛋白酶抑制剂是中国两栖类动物无指盘臭蛙基因编码的一种环状多肽，分子量1951.38道尔顿，等电点9.51，无酶活性，无指盘臭蛙丝氨酸蛋白酶抑制剂全序列为：NH2-AVNIPFKVHFRCKAAFC-COOH，其第12位的半胱氨酸和第17位的半胱氨酸形成分子内二硫键。编码的基因由 301个核苷酸组成，其中编码成熟部分的为第160-213位核苷酸。人工合成的无指盘臭蛙蛋白酶抑制剂具有强烈的丝氨酸蛋白酶抑制活性，作为制备药物肿瘤、胃炎、胰腺炎药物的应用，并且还具有序列简单、合成方便等优点。

A review on oil sardine 1. Distribution, preservation and transportation

Oil sardine (Sardinella longiceps) is widely reported from the Indian Ocean and southeast Asia coasts. It is found, with other less important spp of Sardinella, around both coasts of India. Landings have shown wide variations from yr to yr. Figures were 7412 tons in 1956 and 301,641 tons in 1968. Various possible reasons for this are noted. The main fishery is concentrated in coastal waters 12-15 km from shore in waters up to 15 m deep. The gears used are mostly seine nets. Though the fish has a good protein value, its prices do not compare well to other fish, often due to handling and preservation difficulties. Problems encountered during preservation and transportation of the fish are considered. These include bursting and rancidity.

曲拉通一影响脑内递质及合成酶免疫组化定位‘

目的研究曲拉通一一, 是否会改变脑内递质及合成酶免疫组织化学定位特征, 及是 否与其浓度有关。材料与方法观察了高、低浓度预处理脑切片, 对胆碱乙酞化酶、物质和羊氨基丁酸样免 疫反应神经元胞体及终末, 在树晌内侧隔核、斜带核及海马, 免疫组化定位的影响。并在树殉和猫脑探索了缓冲的 低浓度梯度酒精替代的可能性。结果在树的脑, 当为时, 内侧隔核、斜带核三类神经元胞体染色 变浅, 数量减少, 甚至消失, 而海马及内侧隔核、斜带核的物质同及一氨基丁酸阳性终末明显增加。当为。 时, 以上情况正好相反。以酒精替代, 在树动和猫的海马及 内侧隔核、斜带核能相当好地同时显示不氨基丁酸和物质阳性神经元胞体和终末。结论不同浓度的处理 脑切片, 可能对脑内众多神经活性物质的免疫组化定位特征产生不同影响。提高或降低浓度, 分别有助于显 示神经元终末或胞体。上述梯度酒精替代, 在免疫组化光、电镜研究中, 有助于同时显示阳性神经元胞体和终 末。

黑长臂猿(Hylobates concolor)鸣叫行为研究

黑长臂猿通常在上午鸣叫, 多数发生在9:30分以前, 鸣叫起始时间具季节性变化。黑长臂猿平均每两天鸣叫一次, 日鸣叫发生频次在50%左右, 鸣叫的发生也随季节而变化。鸣叫的持续时间无群体的差异, 但群体在不同季节鸣叫时间长短不一, 一个群体的鸣叫并未引起其它群体的鸣叫。

Fabrication of high-Q film bulk acoustic resonator (FBAR) filters withcarbon nanotube (CNT) electrodes

FBAR devices with carbon nanotube (CNT) electrodes have been developed withthe aim of taking advantage of the low density and high acoustic impedance ofthe CNTs compared to other known materials. The influence of the CNTs on thefrequency response of the FBAR devices was studied by comparing two identicalsets of devices, one set comprised FBARs fabricated with chromium/gold bilayerelectrodes, and the second set comprised FBARs fabricated with CNT electrodes.It was found that the CNTs had a significant effect on attenuating travellingwaves at the surface of the FBARs membranes due to their high elastic stiffness.Finite element analysis of the devices fabricated was carried out using COMSOLMultiphysics, and the numerical results confirmed the experimental resultsobtained. © 2010 IEEE.

蓝氏贾第虫组蛋白的初步研究

贾第虫存在5种组蛋白, 其在两种不同性质电泳系统中的电泳行为与相应的小牛胸腺组蛋白有近似的对应关系。说明贾第虫的组蛋白已发生了分化, 并已初步形成了性质不同的几个组分, 从而在一定程度上支持核小体组蛋白在真核生物的原核祖先阶段就已产生了分化的假说。