Sind südliche Arten in Nord- und Ostsee auf dem Vormarsch?

The long and warm summer 2003 resulted in water temperatures of about 2°C above the average. This promoted the immigration of southern species like the Anchovy, the Sardine, the Red Mullet and different Squid species. The possible conclusions of such invasions concerning the ordinary species of the area are rather contradictory: For the Western Baltic a positive correlation between the occurrence of Anchovy and a good Cod year-class is shown. For the southern North Sea, stomach investigations of Squid make a negative effect on fish eggs and larvae more likely.

Zusammenhang zwischen Schadstoffen aus der Elbe und missgebildeten Fischembryonen in der Deutschen Bucht.

The trends of malformation prevalence in embryos of dab, Limanda limanda, in the southern North Sea after the year 1990 mirrored the drop in major pollutants in the rivers draining into the German Bight. Despite this general decline we detected a pollution event in the southern North Sea in winter 1995/1996 employing the prevalence of malformations in dab embryos as an indicator. An abrupt rise in malformation prevalence in the embryos of dab, corresponded to a dramatic increase in DDT levels in parent fish from the same area, indicating a hitherto unnoticed introduction of considerable quantities of DDT into the system. This input could be traced back to discharges of unknown origen into the River Elbe.

Wassertemperaturen und Häufigkeiten von Mißbildungen pelagischer Fischembryonen

Malformation rates in fish embryos have been monitored for several years in the Southern North Sea. Their occurrence was interpreted to be related to pollution because malformation rates were highest in near coastal waters known to receive high pollution loads. For embryos of all species investigated synchronous trends for the fluctuation of malformation rates over the time were registered in the areas covered with intermediate prevalences at the beginning of the studies in 1984 and maxima in 1987. Thereafter malformation rates of all species decreased significantly followed by an increase in 1996. It was found that a significant negative correlation between surface water temperature and prevalences of malformed embryos of dab (Limanda limanda) and other species existed over time and space. These correlations became increasingly visible with decreasing concentrations of organochlorines in livers of dab. From these findings it is concluded that temperatures possibly predispose developing fish embryos to the impact of pollutants.

Proceedings of the international workshop on the ecology and fisheries for tunas associated with floating objects

It has long been known that tunas frequently associate with floating objects, such as trees washed out to sea during periods of heavy rainfall, and fishermen have taken advantage of this behavior to facilitate the capture of fish. In some coastal areas, such as the Philippines, artisanal fishermen construct anchored fish-aggregating devices (FADs) to attract fish. More recently, large numbers of free-floating FADs have been constructed for deployment by large purse seiners on the high seas. The FADs often can be interrogated by the seiner and located at great distances using radio telemetry and/or GPS (Global Positioning System) technologies. In some cases a fleet of fishing vessels has a tender vessel which deploys and maintains the FADs, and notifies the fishing vessels when fish are seen around them. This workshop was convened by the Inter-American Tropical Tuna Commission and sponsored by Bumble Bee Seafoods, Inc., for the purpose of bringing together scientists and fishermen who have studied the association of tunas with floating objects. Special efforts were made to get participants from all the areas in which tunas associated with floating objects are the targets of fisheries. Thus the "regional review papers" include contributions for the eastern Atlantic, the southern Caribbean Sea, the Indian Ocean, and the eastern and western Pacific Oceans. Many of these reviews and other contributed papers are published in this proceedings volume. Other papers discussed in the workshop were published elsewhere; these papers are cited in the list of background documents in the Report of the Workshop.

Genetic variation of rougheye rockfish (Sebastes aleutianus) and shortraker rockfish (S. borealis) inferred from allozymes

Rougheye rockfish (Sebastes aleutianus) and shortraker rockfish (Sebastes borealis) were collected from the Washington coast, the Gulf of Alaska, the southern Bering Sea, and the eastern Kamchatka coast of Russia (areas encompassing most of their geographic distribution) for population genetic analyses. Using starch gel electrophoresis, we analyzed 1027 rougheye rockfish and 615 shortraker rockfish for variation at 29 proteincoding loci. No genetic heterogeneity was found among shortraker rockfish throughout the sampled regions, although shortraker in the Aleutian Islands region, captured at deeper depths, were found to be significantly smaller in size than the shortraker caught in shallower waters from Southeast Alaska. Genetic analysis of the rougheye rockfish revealed two evolutionary lineages that exist in sympatry with little or no gene f low between them. When analyzed as two distinct species, neither lineage exhibited heterogeneity among regions. Sebastes aleutianus seems to inhabit waters throughout the Gulf of Alaska and more southern waters, whereas S. sp. cf. aleutianus inhabits waters throughout the Gulf of Alaska, Aleutian Islands, and Asia. The distribution of the two rougheye rockfish lineages may be related to depth where they are sympatric. The paler color morph, S. aleutianus, is found more abundantly in shallower waters and the darker color morph, Sebastes sp. cf. aleutianus, inhabits deeper waters. Sebastes sp. cf. aleutianus, also exhibited a significantly higher prevalence of two parasites, N. robusta and T. trituba, than did Sebastes aleutianus, in the 2001 samples, indicating a possible difference in habitat and (or) resource use between the two lineages.

黄海沉积物多细胞趋磁原核生物的特性研究

多细胞趋磁原核生物（Multicellular magnetotactic prokaryotes，MMPs） 是一类由7～45 个含有磁小体的革兰氏阴性细胞聚集而成的球形或者椭圆形 的细胞聚集体，是研究生命起源与进化、细胞分化和生物矿化的模式生物， 目前仅在大西洋沿岸具有一定盐度的层化水体或沉积物中发现。 本文通过光学显微镜和电子显微镜研究了黄海沉积物MMPs 的超微结 构、运动特点和分裂方式等生物学特征，调查了MMPs 的生态分布特征，并 对其尝试培养。 根据形态差别，黄海沉积物的MMPs 可分为花瓣型MMPs（rosette-like MMPs）、菠萝型MMPs（pineapple-like MMPs）和松球型MMPs（pinecone-like MMPs）。花瓣型MMPs 是由23±4 个卵圆形的细胞螺旋形排列而成的球形聚 集体，直径为5.4±0.8 μm，鞭毛周生。细胞内外膜附近有子弹头形/和方形的 铁氧化物型磁小体。菠萝型MMPs 是由39±9 个方形细胞组成的大小为9.6±1.2 μm ×7.8±0.9 μm 的椭圆形聚集体，鞭毛周生。这类MMPs 由多环细胞组成的， 从椭圆体的赤道面向两极，细胞环的直径变小；在每一环内，细胞像书本似 并列相连；相邻两环的细胞为交错式相连。这种结构比花瓣型MMPs 的更为 紧密。菠萝型MMPs 的磁小体均为子弹头形铁氧化物，磁小体的排列与MMPs 的长轴近似平行。松球型MMPs 是由多个长条形的细胞围绕中心的一个凹陷 辐射排列而成的球形聚集体，直径在9.0～14.2 μm 之间。尼罗红和DAPI 染 色发现三种MMPs 均具有脂类颗粒，花瓣型MMPs 和菠萝型MMPs 在聚集体 的表面具有一层外膜，这说明MMPs 的细胞排列具有高度组织性，在一定程 度证明它属于多细胞生物。 花瓣型MMPs 和菠萝型MMPs 分裂时均保持多细胞形式，但花瓣型 MMPs 沿着聚集体的短轴分开，而菠萝型MMPs 沿着长轴分开。两种MMPs 具有MMPs 典型的逃逸运动，花瓣型MMPs 和菠萝型MMPs 的运动速度分别 为55±26 μm/s 和99±50 μm/s。 黄海花瓣型MMPs 的超微结构、运动方式和分裂特点与大西洋沿岸多个 地区发现的MMPs 相似，花瓣型MMPs 可能是MMPs 的优势类群。菠萝型 MMPs 从整体形态、细胞排列和分裂方式上与花瓣型MMPs 显著不同，是一 类新的MMPs。松球型MMPs 是一类尚未报道的MMPs。 对MMPs 的生态分布调查发现，花瓣型MMPs 广泛分布于砂质沉积物中， 最大丰度出现在氧化还原跃层（redoxcline）。菠萝型MMPs 多分布在砾石沉 积物的表层。两种MMPs 占据不同的生态位，暗示着两者可能具有不同的生 理代谢途径。 对MMPs 的培养发现，在实验室内MMPs 可存活8 个月，MMPs 丰度随 着时间变化出现周期性的变化，推测其繁殖周期可能是10～15 天。 本文为太平洋沿岸MMPs 的首次研究，支持MMPs 在全球广泛分布的观 点，并展示了MMPs 的形态多样性。

Study on some biological and feeding factors of spined loach Cobitis taenia from the Babolrud, Talar and Siahrud Rivers

Spined loach, Cobitis taenia, is a predominant fish in the river systems of the southern Caspian Sea basin. Although there is evidence of the geographical divergence of this taxon, but no information is available on morphological differences within the species populations. This study was designed to evaluate some biological factors including; morphometric and meristic characters, length-weight, age-growth, condition factor, diet, reproduction, variation and differentiation, in the Babolrud, Talar and Siahrud Rivers in south of the Caspian Sea basin. Age, sex ratio, fecundity, ova diameter and gonadosomatic index were estimated. Also, regression analyses was tested the relation between fecundity and fish length, weight, gonad weight, and also age. Totally 858 fish of which 721 were matures, were collected from these rivers by electrofishing. 37 morphometric characters, 9 meristic characters and 78 truss network system characters were estimated. Resulats of DFA analysis based on data of morphometric and meristic showd that these populations are highly (94.5%) varios from each other. In discriminate function analysis, the proportion of individuals correctly classified into their original groups was 61%, 65.4% and 86.5% for upstream and downstream, respectively. Clustering based on Euclidean distances among groups of centroids using an UPGMA and also principal component analysis’ results for morphometric data indicated that these populations from these three rivers were clearly distinct from each other. Regression equations between length and weight in these three populations were significantly different from Folton factor (b=3), that showed the fish has a negative Alometric growth process. Condition factor was estimated between 0.8912 to 1.2736 and 0.8131 to 1.4489 for males and females, respectively. Sex ratio (female: male) in these populations was 1.2816:1. The difference between the number of females and males was significant and females were more than males. The female and male specimens reach maturity by Tl more than 40 and 30 mm and at the age of 2+ and 1+, respectively. The mean of ova diameter was 0.5824±0.2882. The spawning took place from May to late July, at the water temperature from 18.7 to 24.0°C. The GSI values average at the beginning of the reproduction period was about 9%, with ranged from 2 to 26% in ripe mature females. The absolute and relative fecundity were 2109±792 and 579±208 respectively. The absolute fecundity was significantly related to body weight and gonads weight. Based on the pattern of gonado-somatic index, it was concluded that this fish has prolong active reproductive period, which is a type of adaptation by short-lived small fishes to environmental conditions. The macroscopic and histological results showed that the female and male have 5 and 4 stages in their maturation process, respectively. The RLG index was about 0.4732, which showed the fish is a carnivorous species. Significant difference was observed between fishes with different length and diet. The main foods of the fish were Trichoptera, Chironomidae larvae and Ephemeroptera which were their prefered food as well, however it was estimated that the food selection and diet are affected by environmental conditions.

Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis

Marine sponges (Porifera) possess an extraordinary diversity of bioactive metabolites for new drug discovery and development. In vitro cultivation of sponge cells in a bioreactor system is very attractive for the sustainable production of sponge-derived bioactive metabolites; however, it is still a challenging task. The recent establishment of sponge primmorphs, multicellular aggregates from dissociated mixed-cell population (MCP), has been widely acknowledged to hold great promise for cultivation in vitro. Here we present a new method to establish an in vitro sponge primmorph culture from archaeocyte-dominant cell population (ADCP) enriched by a Ficoll gradient, rather than a mixed-cell population (MCP). Our rationale is based upon the totipotency (the ability of a cell to differentiate into other cell types) of archaeocyte cells and the different biological functions of various sponge cell types. A sponge, Hymeniacidon perleve collected from the China Yellow Sea was used as a model system for this investigation. Distinct dynamics of primmorph formation were observed while significant increases in DNA synthesis, cell proliferation (up to threefold), and cell growth (up to fourfold) were achieved. Furthermore, a time-dependent spiculogenesis was clearly demonstrated in our longterm culture, indicating high metabolic activity of primmorphs from the ADCP. This new method represents an important step forward to advance sponge cell culture in vitro that may lead to commercial exploitation of sponge-derived drugs. (C) 2003 Wiley Periodicals, Inc.

Polyaniline as marine antifouling and corrosion-prevention agent

Conductive polyaniline was found to have special marine antifouling property. The coating from conducting polyaniline and epoxy resin(or polyurethane) can last 6-9 months in Southern China sea, i.e., less than 10% of the coating surface was fouled during this period. The conducting polyaniline has special synergetic antifouling effect on other antifouling agents like cuprous oxide or 4, 4'-dichlorodiphenyltrichloroethane. The conductivity of the polyaniline was found to be extremely important for its antifouling effect. Moreover, employing aliphatic polyamine as solvent of emeraldine base and curing agent of epoxy resin, a new technique to process corrosion prevention coating containing emeraldine base was developed, therefrom the emeraldine base and epoxy resin was in molecular level blending. This technique was solvent free and extremely effective, i.e., only 1wt% of emeraldine base in the coating can have good corrosion prevention property.

Lack of genetic differentiation in the shrimp Penaeus chinensis in the northwestern pacific

Genetic differentiation of the shrimp Penaeus chinensis in the Yellow Sea and Bohai Sea was investigated using the mitochondrial control region (CR). RFLP of a partial CR segment (613 bp) shows that 106 out of 122 (86.9%) individuals from six sampling localities along the coast of northern China and the west coast of the Korean Peninsula share the same haplotype, and the haplotype frequencies among localities are not significantly different. The findings are further confirmed by sequencing the complete CR. Divergence of the complete CR (992 bp) is less than 1.6% in 14 individuals from the six localities. F-statistics based on RFLP data and the TCS network of sequencing data suggest little genetic differentiation of P. chinensis in the Yellow Sea and Bohai Sea. Mismatch analysis suggests a rapid expansion of P. chinensis population to the Yellow Sea and the Bohai Sea, which probably occurred with the rapid rise in sea level after the last glacial maximum. Despite the lack of genetic heterogeneity, we propose that P. chinensis populations in this region should be treated as separate management units, as fishery management programs have to be applied on a local basis by different governments.

Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic

ISSR analysis was used to investigate genetic variations of 184 haploid and diploid samples from nine North Atlantic Chondrus crispus Stackhouse populations and one outgroup Yellow Sea Chondrus ocellatus Holmes population. Twenty-two of 50 primers were selected and 163 loci were scored for genetic diversity analysis. Genetic diversity varied among populations, percentage of polymorphic bands (PPB) ranged from 27.0 to 55.8%, H(Nei's genetic diversity) ranged from 0.11 to 0.20 and I(Shannon's information index) ranged from 0.16 to 0.30. Estimators PPB, H and I had similar values in intra-population genetic diversity, regardless of calculation methods. Analysis of molecular variance (AMOVA) apportioned inter-population and intra-population variations for C crispus, showing more genetic variance (56.5%) occurred in intra-population, and 43.5% variation among nine populations. The Mantel test suggested that genetic differentiation between nine C. crispus populations was closely related with geographic distances (R = 0.78, P = 0.002). Results suggest that, on larger distance scale (ca. > 1000 km), ISSR analysis is useful for determining genetic differentiations of C crispus populations including morphologically inseparable haploid and diploid individuals. (c) 2007 Elsevier B.V. All rights reserved.

Comparative growth and viability of hybrids between two populations of Chinese shrimp (Fennropenaeus chinensis)

Fenneropenaeus chinensis is confined to the Yellow Sea and Bohai Sea in China and the West Coast of the Korean Peninsula. Intra- and intercross populations were produced between Rushany (YP) and Korean (KN) populations. Seven traits were recorded. The heterosis of hybrids was computed and comparison between treatments was performed by ANOVA. At the fourth month after post-larvae, six indexes of growth trait and viability showed a range of heterosis, ranging from 0.514% to 14.950%. At the fifth month after post-larvae, six indexes of growth trait and viability ranged from -9.000% to 19.090%. The negative heterosis was observed in CL, HST and viability. The heterosis of KN female xYP male tended generally to increase as the age of the Chinese shrimp increased while the heterosis of YP female xKN male tended to decrease. The results indicated that the viability of reciprocal hybrids were not significantly different (P > 0.05) from their parents during the experiment. The result of ANOVA indicated that the F1 hybrids were significantly different (P < 0.05) in WST and TW at the fourth month. The multiple comparisons of LSD test indicated that the hybrids of YP female xKN male were significantly different (P < 0.05) from their parents in TW. The hybrids of YP female xKN male were significantly different (P < 0.05) from the other three combinations in WST. At the fifth months, the F1 hybrids had significant difference (P < 0.05) in body weight while other traits showed no significant differences (P > 0.05) from their parents. The multiple comparisons of LSD test indicated that the hybrids of KN female xYP male were significantly different (P < 0.05) from the KN parents in TW. The results indicate that in experimental conditions, the F-1 hybrids created from two populations of Chinese shrimp showed a certain level of heterosis for growth performance and viability. The crossing scheme may improve growth performance and viability in Chinese shrimp, but the improvement may be limited because effective crossbreeding requires the maintenance of pure, preferably inbred, lines and possibly involves specialized sire and dam lines. Therefore, the exploitation of heterosis through single crossbreeding in Chinese shrimp is of limited utility in practical commercial shrimp aquaculture in spite of the potential of significant heterosis. The crossbreeding of different populations can be applied in the establishment of base populations.

Black carbon and polycyclic aromatic hydrocarbons (PAHs) in surface sediments of China's marginal seas

This study investigates the distribution of black carbon (BC) and its correlation with total polycyclic aromatic hydrocarbons (I PAH) pound in the surface sediments of China's marginal seas. BC content ranges from < 0.10 to 2.45 mg/g dw (grams dry weight) in the sediments studied, and varied among the different coastal regions. The Bohai Bay sediments had the highest BC contents (average 2.18 mg/g dw), which comprises a significant fraction (27%-41%) of the total organic carbon (TOC) preserved in the sediments. In comparison, BC in the surface sediments of the North Yellow Sea, Jiaozhou Bay, East China Sea and the South China Sea is less abundant and accounted for an average of 6%, 8%, 14% and 5%, respectively, of the sedimentary organic carbon pool. The concentration of I PAH pound in the surface sediments ranges from 41 to 3 667 ng/g dw and showed large spatial variations among the sampling sites of different costal regions. The Bohai Bay has the highest I PAH pound values, ranging from 79 to 3 667 ng/g dw. This reflects the high anthropogenically contaminated nature of the sediments in the bay. BC is positively correlated to TOC but a strong correlation is not found between BC and I PAH pound in the surface sediments studied, suggesting that BC and PAHs preserved in the sediments are derived from different sources and controlled by different biogeochemical processes. Our study suggests that the abundance of BC preserved in the sediments could represent a significant sink pool of carbon cycling in China's marginal seas.

Paleoenvironmental change in the middle Okinawa Trough since the last deglaciation: Evidence from the sedimentation rate and planktonic foraminiferal record

Well-dated, high-resolution records of planktonic foraminifera and oxygen isotopes from two sediment cores, A7 and E017, in the middle Okinawa Trough reveal strong and rapid millennial-scale climate changes since similar to 18 to 17 thousand years before present (kyr B.P.). Sedimentation rate shows a sudden drop at similar to 11.2 cal. kyr B.P. due to a rapid rise of sea level after the Younger Dryas (YD) and consequently submergence of the large continental shelf on the East China Sea (ECS) and the retreat of the estuary providing sediment to the basin. During the last deglaciation, the relative abundance of warm and cold species of planktonic foraminifera fluctuates strongly, consistent with the timing of sea surface temperature (SST) variations determined from Mg/Ca measurements of planktonic foraminifera from one of the two cores. These fluctuations are coeval with climate variation recorded in the Greenland ice cores and North Atlantic sediments, namely Heinrich event 1 (H1), Bolling-Allerod (B/A) and YD events. At about 9.4 kyr B.P., a sudden change in the relative abundance of shallow to deep planktonic species probably indicates a sudden strengthening of the Kuroshio Current in the Okinawa Trough, which was synchronous with a rapid sea-level rise at 9.5-9.2 kyr B.P. in the ECS, Yellow Sea (YS) and South China Sea (SCS). The abundance of planktonic foraminiferal species, together with Mg/Ca based SST, exhibits millennial-scale oscillations during the Holocene, with 7 cold events (at about 1.7, 2.3-4.6, 6.2, 7.3, 8.2, 9.6, 10.6 cal. kyr BP) superimposed on a Holocene warming trend. This Holocene trend, together with centennial-scale SST variations superimposed on the last deglacial trend, suggests that both high and low latitude influences affected the climatology of the Okinawa Trough. (c) 2006 Elsevier B.V. All rights reserved.

Measurements of ocean wave and current field using dual polarized X-band radar

A new ocean wave and sea surface current monitoring system with horizontally-(HH) and vertically-(VV) polarized X-band radar was developed. Two experiments into the use of the radar system were carried out at two sites, respectively, for calibration process in Zhangzi Island of the Yellow Sea, and for validation in the Yellow Sea and South China Sea. Ocean wave parameters and sea surface current velocities were retrieved from the dual polarized radar image sequences based on an inverse method. The results obtained from dual-polarized radar data sets acquired in Zhangzi Island are compared with those from an ocean directional buoy. The results show that ocean wave parameters and sea surface current velocities retrieved from radar image sets are in a good agreement with those observed by the buoy. In particular, it has been found that the vertically-polarized radar is better than the horizontally-polarized radar in retrieving ocean wave parameters, especially in detecting the significant wave height below 1.0 m.