Reverse Offshoring of Services: The New Wave of Emerging Offshorers. ACES Working Papers, August 2010

Offshore outsourcing of services accelerated at the end of 1990s in developed countries. In recent years, developing countries have also offshored services, not only to developed countries but also to other developing countries. Yet, to date little attention has been paid to the emergence of this reverse offshoring. The focus of this research is on the determinants of reverse offshoring of services; as such we will investigate what drives these firms from developing countries to offshore services.

Epigenetic engineering shows H3K4me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore

Kinetochores assemble on distinct 'centrochromatin' containing the histone H3 variant CENP-A and interspersed nucleosomes dimethylated on H3K4 (H3K4me2). Little is known about how the chromatin environment at active centromeres governs centromeric structure and function. Here, we report that centrochromatin resembles K4-K36 domains found in the body of some actively transcribed housekeeping genes. By tethering the lysine-specific demethylase 1 (LSD1), we specifically depleted H3K4me2, a modification thought to have a role in transcriptional memory, from the kinetochore of a synthetic human artificial chromosome (HAC). H3K4me2 depletion caused kinetochores to suffer a rapid loss of transcription of the underlying α-satellite DNA and to no longer efficiently recruit HJURP, the CENP-A chaperone. Kinetochores depleted of H3K4me2 remained functional in the short term, but were defective in incorporation of CENP-A, and were gradually inactivated. Our data provide a functional link between the centromeric chromatin, α-satellite transcription, maintenance of CENP-A levels and kinetochore stability.

A quantitative reverse-transcriptase PCR assay for the assessment of drug activities against intracellular Theileria annulata schizonts.

Intracellular schizonts of the apicomplexans Theileria annulata and Theileria parva immortalize bovine leucocytes thereby causing fatal immunoproliferative diseases. Buparvaquone, a hydroxynaphthoquinone related to parvaquone, is the only drug available against Theileria. The drug is only effective at the onset of infection and emerging resistance underlines the need for identifying alternative compounds. Current drug assays employ monitoring of proliferation of infected cells, with apoptosis of the infected host cell as a read-out, but it is often unclear whether active compounds directly impair the viability of the parasite or primarily induce host cell death. We here report on the development of a quantitative reverse transcriptase real time PCR method based on two Theileria genes, tasp and tap104, which are both expressed in schizonts. Upon in vitro treatment of T. annulata infected bovine monocytes with buparvaquone, TaSP and Tap104 mRNA expression levels significantly decreased in relation to host cell actin already within 4 h of drug exposure, while significant differences in host cell proliferation were detectable only after 48-72 h. TEM revealed marked alterations of the schizont ultrastructure already after 2 h of buparvaquone treatment, while the host cell remained unaffected. Expression of TaSP and Tap104 proteins showed a marked decrease only after 24 h. Therefore, the analysis of expression levels of mRNA coding for TaSP and Tap104 allows to directly measuring impairment of parasite viability. We subsequently applied this method using a series of compounds affecting different targets in other apicomplexan parasites, and show that monitoring of TaSP- and Tap104 mRNA levels constitutes a suitable tool for anti-theilerial drug development.

PBI reverse osmosis membrane for chromuim plating rinse water /

Mode of access: Internet.

Combined reverse osmosis and freeze concentration of bleach plant effluents /

Mode of access: Internet.

Wastewater demineralization by tubular reverse osmosis process /

Mode of access: Internet.

Demineralization of carbon-treated secondary effluent by spiral-wound reverse osmosis process /

Mode of access: Internet.

The ordinances of the Clothworkers' Company together with those of the ancient guilds or fraternities of the fullers & shearmen of the city of Lon don, transcribed from the originals in the possession of the Company.

Includes index.

Proceedings, reverse mortgages : improving housing market liquidity ; March 2, 1993 /

Mode of access: Internet.

The Triumph of Fame (reverse)

Giovanni di ser Giovanni Guidi; D: 3 ft. 1/2 in. (painted surface); tempera, silver, and gold on wood

Francesco d'Este (born about 1430, died after 1475), reverse, Este family coat of arms and crest

Rogier van der Weyden; 11 47/64 in.x 7 63/64 in. (painted surface); oil on wood

Application of positive guidance at a reverse curve/narrow bridge site in Washington state. Final report.

Federal Highway Administration, Demonstration Projects Division, Arlington, Va.