Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis

Centrioles organize the centrosome, and accurate control of their number is critical for the maintenance of genomic integrity. Centrioles duplicate once per cell cycle, and duplication is coordinated by Polo-like kinase 4 (Plk4). We previously demonstrated that Plk4 accumulation is autoregulated by its own kinase activity. However, loss of heterozygosity of Plk4 in mouse embryonic fibroblasts has been proposed to cause cytokinesis failure as a primary event, leading to centrosome amplification and gross chromosomal abnormalities. Using targeted gene disruption, we show that human epithelial cells with one inactivated Plk4 allele undergo neither cytokinesis failure nor increase in centrosome amplification. Plk4 is shown to localize exclusively at the centrosome, with none in the spindle midbody. Substantial depletion of Plk4 by small interfering RNA leads to loss of centrioles and subsequent spindle defects that lead to a modest increase in the rate of cytokinesis failure. Therefore, Plk4 is a centriole-localized kinase that does not directly regulate cytokinesis.

Las Casas as a bishop : a new interpretation based on his holograph petition in the Hans P. Kraus Collection of Hispanic American manuscripts = Las Casas, obispo : una nueva interpretación basada en su petición autógrafa conservada en la Colección Hans P. Kraus de manuscritos hispanoamericanos /

Includes indexes.

Main Street North from Liberty Street - Ann Arbor

Plates. 12 pts. in 1 v. Publication information: Chicago : The W. H. Parish publishing co., 1893. Digital image from copy neg in Stugis collection.

A list of the Lancashire wills, proved within the archdeaconry of Richmond and now preserved in the Court of Probate at Lancaster, from 1748 to [1812]. Also a list of the wills proved in the peculiar of Halton from A.D. 1615 to [1812].

Vol. 2 contains the report of the Record Society for the year 1911/12.

Abstract of the answers and returns made pursuant to an act, passed in the first year of the reign of His Majesty King George IV, intituled, "An act for taking an account of the population of Great Britain, and of the increase or diminution thereof" ... M.DCCC.XXI.

Signed: Jno. Rickman.

The case of seduction : being, an account of the late proceedings at Paris ... against the reverend Abbée, Claudius Nicholas des Rues, for committing rapes upon 133 virgins. ... /

The unnumbered leaf is the contents leaf for 'The case of seduction'

The registry of St. Mary, Leicester, in the county of Leicester.

Mode of access: Internet.

Essai de monographie paroissiale: St.-Georges d'Henryville et la seigneurie de Noyan.

Mode of access: Internet.

Answers to inquiries respecting Frome Selwood, in Somersetshire,

Mode of access: Internet.

Rye's monographs of Norwich hamlets. no. 1-[5] ...

Paged continuously.

Pamphlets on English architecture assembled by George Gilbert Scott.

Vol. 6, unanalyzed publications, cont. Records of Buckinghamshire, by the Architectural and Archaeological Society for the County of Buckingham (1854). Includes articles: Ancient British gold coins found in Whaddon Chase. Antiquities of the Chiltern Hills / W.J. Burgess. Aylesbury Church in 1848 : architect's report / Geo. Gilbert Scott. A letter from G.G. Scott, Esq., on the supposed Saxon work at Iver and at Wing. Church bells / Robert Eaton Batty. The desecrated churches of Buckinghamshire [cont.]. Drayton Beauchamp / by W. Hastings Kelke. St. Mary's, Ashendon, Bucks. / by Frederick George Lee. Earth-works at Hampden and Little Kimble / by Boughey Burgess. Hilldesden Church -- Transactions of the St. Albans Architectural and Archaeological Society (1851). Includes article: Notice of a seal formed of bone, discovered in the Abbey Church, St. Albans ... / by Albert Way.

The complete works of F. Marion Crawford.

v. 1. Mr. Isacs -- v. 2. Doctor Claudius -- v. 3. To leeward -- v. 4. A roman singer -- v. 5. An American politician -- v. 6. Marzio's crucifix. Zoroaster -- v. 7. A tale of a lonely parish -- v. 8. Paul Patoff -- v. 9. Love in idleness. Marion Darche -- v. 10. Saracinesca -- v. 11. Sant' Ilario -- v. 12. Don Orsino -- v. 13. Corleone -- v. 14. With the immortals -- v. 15. Greifenstein -- v. 16. A cigarett-maker's romance. Khaled -- v. 17. The witch of Prague -- v. 18. The three fates -- v. 19. Taquisara -- v. 20. The children of the king -- v. 21. Pietro Ghisleri -- v. 22. Katherine Lauderdale -- v. 23. The Ralstons -- v. 24-25. Casa Braccio -- v. 26. Adam Johnstone's son. A rose of yesterday -- v. 27. Via crucis -- v. 28. In the palace of the king -- v. 29. Marietta -- v. 31. The heart of Rome -- v. 32. Whosoever shall offens.

Abstract of the answers and returns made pursuant to an act, passed in the eleventh year of the reign of His Majesty King George IV, intituled, "An act for taking an account of the population of Great Britain, and of the increase of diminution thereof", M.DCCC.XII.

Preface also published separately with title: Preface to the abstract of the population of Great Britain. M.DCCC.XXXI.

The life of Richard Steele,

Appendices (v. 2, p. [347]-428): I. The Steeles of Cheshire, and others. II. Fords of St. Michael's parish, Barbadoes. III. Performances of Steele's plays. IV. Music for Steele's songs.

Survey of London /

Later vols. published by the Athlone Press for the Greater London Council.