Compostagem de resíduos sólidos de frigorífico

Composting has been a viable and efficient alternative treatment to agroindustrial waste. This experiment was installed with the objective of analyzing the process of composting for slaughterhouse waste. Twelve piles of composting were prepared, using slaughterhouse waste, wheat straw and wood sawdust. The process was evaluated by daily temperature monitoring, observations of the occurrence of undesirable parameters (bad smell and/or ammoniacal smell, grease formation and presence of grubs and flies), as well as the capacity of recycling nutrients. The undesirable parameters were observed, on average, for the first five days after pile building; the temperatures increased, reaching 70 °C; chemical composition of compost showed relevant contents of macro and micronutrients, a high recycling potential. Utilization of impermeable floor and roof are recommended during the composting. The turning frequency used (15 days after pile building followed by turning) was adequate. The best relation of weight was 7.2 kg of waste for each kg of straw and 16.6 kg of waste for each kg of sawdust.

Pasteuriza̧ão de turfa brasileira para o cultivo de Agaricus brasiliensis

Agaricus brasiliensis is a Brazilian basidiomycete which has been cultivated and consumed around the world as a therapeutic food. Casing layer is one of the most important steps on A. brasiliensis cultivation and European peat is the most used casing layer on Agaricus bisporus cultivation. Besides the importance of it on mushroom cultivation the peat import could be a risk of exotic organism introduction. Alternative as Brazilian peat is barely used for mushroom growers in Brazil. Thus, the objective of this work was to evaluate Brazilian peat with and without pasteurization as casing layer on A. brasiliensis cultivation. The fungus was previously grown on wheat grains and transferred to a substratum prepared by composted traditional method. After mycelium colonization of the substratum a pasteurized or non pasteurized Brazilian peat (casing layer) was added. It was concluded that pasteurization of the casing layer increases in 30% yield after 65 days of cultivation. There is no difference of yield for pasteurized and non pasteurized casing layer until 30 days of cultivation. An increase of flies is observed in non pasteurized casing layer. The production flush is easily perceived with pasteurized casing layer but not with non pasteurized casing layer.

Bait and habitat preferences, and temporal variability of social wasps (Hymenoptera: Vespidae) attracted to vertebrate carrion

In addition to feeding on carrion tissues and fluids, social wasps can also prey on immature and adult carrion flies, thereby reducing their populations and retarding the decomposition process of carcasses. In this study, we report on the occurrence and behavior of social wasps attracted to vertebrate carrion. The collections were made monthly from September 2006 to October 2007 in three environments (rural, urban, and forest) in six municipalities of southeast Brazil, using baited bottle traps. We collected Agelaia pallipes (Olivier, 1791) (n = 143), Agelaia vicina (Saussure, 1854) (n = 106), Agelaia multipicta (Haliday, 1836) (n = 18), and Polybia paulista Ihering, 1896 (n = 3). The wasps were observed feeding directly on the baits and preying on adult insects collected in the traps. Bait and habitat associations, temporal variability of social wasps, and possible forensic implications of their actions are discussed. © 2011 Entomological Society of America.

Flutuação populacional e infestação de mosca-das-frutas (Diptera: Tephritidae) em função do sistema produtivo de goiaba

The knowledge about population fluctuation of fruit flies (Diptera: Tephritidae) is an important tool to be adopted in pest control. Thus, this research aimed to analyse the population fluctuation of adults of fruit flies and the infestation of larvae in fruits depending on the conventional and organic agricultural systems of guava production in the 2010/2011 harvest. For monitoring the adults of fruit flies, five yellow sticky traps were installed in each system. The infestation percentage and the calculation of the ratio number of larvae/number of fruits were determined at harvest by harvesting 10 mature fruits/plant, in ten plants, for each system. In organic system predominated Anastrepha spp., principally in the period when the fruits were ripening or ripe, represented by elevated infestation percentage, while the conventional system presented low population density of fruit flies, with reduced larvae infestation inside the fruits.

Asymptomatic dogs are highly competent to transmit Leishmania (Leishmania) infantum chagasi to the natural vector

We evaluated the ability of dogs naturally infected with Leishmania (Leishmania) infantum chagasi to transfer the parasite to the vector and the factors associated with transmission. Thirty-eight infected dogs were confirmed to be infected by direct observation of Leishmania in lymph node smears. Dogs were grouped according to external clinical signs and laboratory data into symptomatic (n= 24) and asymptomatic (n= 14) animals. All dogs were sedated and submitted to xenodiagnosis with F1-laboratory-reared Lutzomyia longipalpis. After blood digestion, sand flies were dissected and examined for the presence of promastigotes. Following canine euthanasia, fragments of skin, lymph nodes, and spleen were collected and processed using immunohistochemistry to evaluate tissue parasitism. Specific antibodies were detected using an enzyme-linked immunosorbent assay. Antibody levels were found to be higher in symptomatic dogs compared to asymptomatic dogs (p= 0.0396). Both groups presented amastigotes in lymph nodes, while skin parasitism was observed in only 58.3% of symptomatic and in 35.7% of asymptomatic dogs. Parasites were visualized in the spleens of 66.7% and 71.4% of symptomatic and asymptomatic dogs, respectively. Parasite load varied from mild to intense, and was not significantly different between groups. All asymptomatic dogs except for one (93%) were competent to transmit Leishmania to the vector, including eight (61.5%) without skin parasitism. Sixteen symptomatic animals (67%) infected sand flies; six (37.5%) showed no amastigotes in the skin. Skin parasitism was not crucial for the ability to infect Lutzomyia longipalpis but the presence of Leishmania in lymph nodes was significantly related to a positive xenodiagnosis. Additionally, a higher proportion of infected vectors that fed on asymptomatic dogs was observed (p= 0.0494). Clinical severity was inversely correlated with the infection rate of sand flies (p= 0.027) and was directly correlated with antibody levels (p= 0.0379). Age and gender did not influence the transmissibility. Our data show that asymptomatic dogs are highly infective and competent for establishing sand fly infection, indicating their role in maintaining L. (L.) infantum chagasi cycle as well as their involvement in VL spreading in endemic areas. © 2013 Elsevier B.V.

Resistance of beef cattle of two genetic groups to ectoparasites and gastrointestinal nematodes in the state of São Paulo, Brazil

The resistance to infestations by ectoparasites and infections by gastrointestinal nematodes was studied in 45 animals (males and females) of two genetic groups: purebred Nelore (NI, n=28) and Three-Cross (1/2 Angus+1/4 Canchim+1/4 Nelore - TC, n=17). The animals were monitored for 24months, during which they were left to graze in tropical pastures without receiving treatment for parasites. Each month the animals were examined for infestations by external parasites, to count the numbers of cattle ticks Rhipicephalus microplus with diameter greater than 4.5mm present on the left side, horn flies (Haematobia irritans) present in the lumbar region and botfly larvae (Dermatobia hominis) present on the entire body. The H. irritans counts were performed with the aid of digital photographs. At the time of examination, fecal samples were collected to count the eggs per gram (EPG) and to perform coprocultures, and peripheral blood samples were drawn to determine the packed cell volume (PCV) and to count the eosinophils. For statistical analysis, the count data were transformed into log10 (n+1), where n is the number of parasites. For PCV, significant effects (P<0.05) were found for collection month (CO), genetic group (GG) and gender (SX), with means and respective standard errors of 41.5±0.65% for the NI animals, 39.3±0.83% for the TC, 41.5±0.72% for the females and 39.3±0.77% for the males. Regarding the eosinophil counts, only the effect of sex was significant (P<0.01), with means and respective standard errors of 926.0±46.2/μL, for males and 1088.0±43.8/μL of blood, for females. The NI animals presented lower mean counts for all the external parasites compared to the TC animals (P<0.01). For ticks, the transformed means followed by standard errors for the NI and TC animals were 0.06±0.01 and 0.34±0.02, while for horn flies these were 0.92±0.05 and 1.36±0.06 and for botfly larvae they were 0.05±0.03 and 0.45±0.05, respectively. The average EPG values were only influenced by CO (P<0.01). The coprocultures revealed the presence of the following endoparasites: Haemonchus spp., Cooperia spp., Oesophagostomum spp. and Trichostrongylus spp., the last in smaller proportion. There were no significant differences between the genetic groups for the endoparasite loads, except for Cooperia spp., which were present in greater number (P<0.05) in the NI group. The results obtained in this experiment confirm previous findings of greater susceptibility of the Nelore breed to Cooperia spp. and high resistance to ectoparasites. © 2013 Elsevier B.V.

Interações interespecíficas e comportamentais de moscas-varejeiras: abordagens ecológicas e médico-legais envolvendo populações naturais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção e estudo sobre o efeito da metanfetamina e do ecstasy no desenvolvimento de imaturos de três espécies de Chrysomya (Diptera: Calliphoridae) de importância forense

Pós-graduação em Biologia Geral e Aplicada - IBB

Análise faunística de dípteros necrófagos: ecologia e aplicação forense

Pós-graduação em Biologia Geral e Aplicada - IBB

Avaliação do potencial tripanocida dos óleos essenciais de Melampodium divaricatum e Hedychium coronarium e dos extratos de Polygonum ferrugineum em cepas de Trypanosoma brucei

Pós-graduação em Biotecnologia - IQ

CD95 (FAS) e CD178 (FASL) induzem apoptose em células CD4+ E CD8+ do sangue periférico e esplênicas em cães naturalmente infectados por Leishmania spp: Kathlenn Liezbeth Oliveira Silva. -

Pós-graduação em Ciência Animal - FMVA

Aspectos bioecológicos de insetos associados à goiabeira em sistemas de produção convencional e orgânico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Bioecologia de Anastrepha fraterculus (Wiedemann, 1830) (Diptera: Tephritidae) em videira

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Moscas frugívoras, lepidópteros desfolhadores e seus parasitóides (Hymenoptera) associados a cultivo de café, em Cravinhos, SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Dinâmica populacional de moscas-das-frutas e de Triozoida limbata (Hemiptera: Psyllidae) e danos de Costalimaita ferruginea (Coleoptera: Chrysomelidae) e de T. limbata em pomar de goiaba submetido a sistema de racionalização de inseticidas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)