Synthesis, cytotoxic activity and DNA interaction of Pd(II) complexes bearing N′-methyl-3,5-dimethyl-1-thiocarbamoylpyrazole

A new series of complexes of general formulae [PdX2(tmdmPz)] {X = Cl (1), Br (2), I (3), SCN (4); tmdmPz = N′-methyl-3,5-dimethyl-1- thiocarbamoylpyrazole} have been synthesized and characterized by elemental analysis, molar conductivities, IR, 1H and 13C{ 1H} NMR spectroscopy. In these complexes, the tmdmPz coordinates to Pd(II) center as a neutral N,S-chelating ligand. The geometries of the complexes have been optimized with the DFT method. Cytotoxicity evaluation against LM3 (mammary adenocarcinoma) and LP07 (lung adenocarcinoma) cell lines indicated that complexes 1-4 were more active than cisplatin. The binding of the complexes with a purine base (guanosine) was investigated by 1H NMR and mass spectrometry, showing that the coordination of guanosine occurs through N7. Electrophoretic DNA migration studies showed that all of them modify the DNA tertiary structure. © 2013 Elsevier Ltd. All rights reserved.

Effects of dietary clinoptilolite and calcium levels on the performance and egg quality of commercial layers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Immunohistochemistry of the uterine cervix of rats bearing the Walker 256 tumor treated with copaiba balsam

ABSTRACT: PURPOSE: To investigate the immunohistochemistry of the uterine cervix of 20 Wistar rats (Rattus norvegicus) bearing the Walker 256 tumor, treated with copaiba oil (Copaifera officinalis). METHODS: The animals were grouped into four subgroups, with five rats each: the GCT and GCopT received distilled water and topically copaiba, respectively, while the GCG and GCopG received distilled water and copaiba by gavage, respectively. The substances were administered for nine days. On the 12th day, after euthanasia, the tumor pieces were sent to the identification of T CD4+, T CD8+ and Natural Killer cells. RESULTS: It was found that the pattern of expression for specific markers of phenotypes of cells involved in tumor immune response was similar in all groups, regardless the administration way of copaiba oil (topical or gavage). CONCLUSION: Copaiba balsam, administered either topically or by gavage, did not alter the pattern of tumor immune response in rats bearing Walker 256 Tumor.

Petrography of gypsum-bearing facies of the Codó Formation (Late Aptian), Northern Brazil

Neste trabalho, é apresentado um estudo original e detalhado enfocando os aspectos petrográficos dos evaporitos de depósitos aptianos superiores expostos no sul e leste da Bacia de São Luís-Grajaú. O objetivo é o estabelecimento de critérios que permitam distinguir entre evaporitos primários e secundários, além da reconstrução de sua evolução pós-deposicional. Sete fases de evaporitos foram reconhecidas: 1. gipsita em chevron; 2. gipsita ou anidrita nodular a lenticular; 3. gipsita fibrosa a acicular; 4. gipsita em mosaico; 5. gipsita brechada a gipsarenito; 6. anidrita ou gipsita pseudo-nodular; e 7. gipsita em rosetas. As três primeiras fases apresentam características petrográficas condizentes com origem primária. Agipsita fibrosa a acicular e a gipsita em mosaico foramformadas por substituições de gipsita primária, com origem provável nos estágios iniciais da diagenêse, portanto ainda sob influência do ambiente deposicional. Estas morfologias de gipsita estão relacionadas com a fácies de evaporito laminado, tendo sido formadas por substituição, porém sem afetar a estruturação primária. A gipsita ou anidrita pseudo-nodular originou-se pela mobilização de soluções sulfatadas durante ou após soterramento, provavelmente associada à halocinese. A gipsita em rosetas, que intercepta todas as outras variedades de gipsita, representa o ultimo estágio de formação de evaporitos na área de estudo, tendo resultado de soluções intraestratais ou de águas superficiais durante intemperismo.

Digestible lysine levels for laying hens and their effects on egg quality

Egg quality of semi-heavy laying hens fed on low protein diets (14.0% CP) and on different lysine levels is evaluated, while maintaining the same ratio of digestible amino acids / digestible lysine. Four hundred and twenty commercial strain Isa Brown laying hens, 28 weeks old, were divided into 42 experimental plots. A completely randomized design with six treatments and seven replicates was employed in four production cycles of 28 days each. Treatments comprised Control - 16.92% CP; 0.750% digestible lysine. Treatments 1 to 5, with CP levels 14% and digestible lysine levels 0.600, 0.675, 0.750, 0.825 and 0.900% respectively. Levels of Treatments 1 and 2 (0.546 and 0.640% digestible Met + Cys / 0.600 and 0.675% digestible lysine) provided smaller egg size. On the other hand, eggs had higher shell percentage when compared to control diet. When compared to other digestible amino acids, digestible lysine requirement may be estimated at 0.750% in a diet with 14% CP, which corresponds to the average daily intake of 876 mg dig. lysine hen-1 day-1 and 798 mg dig. Met + Cys hen-1 day-1, without jeopardizing performance and eggs' internal and external quality.

Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in cotton: vertical distribution of egg masses, effects of adult density and plant age on oviposition behavior

O trabalho teve por objetivo determinar, em casa-de-vegetação, a densidade adequada de adultos, a idade ideal das plantas e a distribuição vertical de ovos nas diferentes partes da planta, visando à realização de estudos de resistência e à melhoria das práticas de manejo de Spodoptera frugiperda em algodoeiro. Os experimentos de oviposição de S. frugiperda em relação à densidade de adultos, relação entre plantas de algodoeiro e oviposição de S. frugiperda e não preferência para oviposição de S. frugiperda em variedades de algodoeiro foram realizados com plantas da variedade de algodoeiro BRS Ita 90. A não preferência (antixenose) para oviposição foi acompanhada nas variedades FiberMax 966, FiberMax 977, DeltaOpal, DeltaPenta, Acala 90, Coodetec 408, Coodetec 409, Coodetec 410, BRS Cedro, BRS Ipê, BRS Aroeira, IPR 96, IPR 20, BRS Araçá, IAC 24 e BRS Ita 90. Concluiu-se que S. frugiperda prefere ovipositar em plantas com cerca de 60 dias de idade, na superfície inferior das folhas situadas no terço superior dos testes de plantas sob condições de estufa. Uma densidade de, pelo menos, três pares de adultos S. frugiperda por planta foi suficiente para realizar testes de não preferência para oviposição em casa de vegetação. As variedades Coodetec 408, BRS Aroeira, BRS Araçá, BRS Ita 90 e DeltaPenta apresentaram resistência do tipo não preferência para oviposição de S. frugiperda. Em regiões com altas infestações de S. frugiperda, seria prudente para o cultivo utilizar a variedade de algodão BRS Ita 90.

Effect of feeding whole linseed as a source of polyunsaturated fatty acids on performance and egg characteristics of laying hens kept at high ambient temperature

The present study was conducted to evaluate the effects of feeding whole linseed on the laying performance and egg characteristics in laying hens kept at high ambient environmental temperatures (average 34 degrees C; the diurnal temperature range 26 degrees C to 41 degrees C). Two hundred and forty 38-wk-old white Leghorn laying hens were fed diets containing 0, 5, 10 or 15% whole linseed (as a source of n-3 polyunsaturated fatty acids) for a period of 12 weeks. Egg production was recorded daily, while feed intake and egg characteristics were monitored on weekly basis. The results of the study demonstrated that egg production and feed intake decreased, while feed conversion ratio (FCR) per dozen of eggs increased (p <= 0.05) with increasing dietary linseed levels. Egg weight, yolk weight, albumen height, eggshell weight, and eggshell thickness were not influenced (p > 0.05) by linseed levels in the diets offered to the laying hens. The results of the present trial suggest that feeding linseed to the laying hens in hot climates has no detrimental effects on egg characteristics, but has suppressive effects on egg production, feed intake and feed efficiency of laying hens.

Cytogenetic analysis in three Bryconamericus species (Characiformes, Characidae): first description of the 5S rDNA-bearing chromosome pairs in the genus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of Egg Rearing Temperature and Storage Time on the Biological Characteristics of the Predatory Stink Bug Podisus nigrispinus (Hemiptera: Pentatomidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Reproductive variability of the Amazon River prawn, Macrobrachium amazonicum (Caridea, Palaemonidae): influence of life cycle on egg production

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Synthesis and biological evaluation of ternary silver compounds bearing N,N-chelating ligands and thiourea: X-ray structure of [{Ag(bpy) (mu-tu)}(2)](NO3)(2) (bpy=2,2 '-bipyridine; tu = thiourea)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Higher Insulin Sensitivity and Impaired Insulin Secretion in Cachetic Solid Ehrlich Tumour-Bearing Mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)