Role of an indole-thiazolidine molecule PPAR pan-agonist and COX inhibitor on inflammation and microcirculatory damage in acute gastric lesions

The present study aimed to show the in vivo mechanisms of action of an indole-thiazolidine molecule peroxisome-proliferator activated receptor pan-agonist (PPAR pan) and cyclooxygenase (COX) inhibitor, LYSO-7, in an ethanol/HCl-induced (Et/HCl) gastric lesion model. Swiss male mice were treated with vehicle, LYSO-7 or Bezafibrate (p.o.) 1 hour before oral administration of Et/HCl (60%/0.03M). In another set of assays, animals were injected i.p. with an anti-granulocyte antibody, GW9962 or L-NG-nitroarginine methyl ester (L-NAME) before treatment. One hour after Et/HCl administration, neutrophils were quantified in the blood and bone marrow and the gastric microcirculatory network was studied in situ. The gastric tissue was used to quantify the percentage of damaged area, as well as myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS) protein and PPARγ protein and gene expression. Acid secretion was evaluated by the pylorus ligation model. LYSO-7 or Bezafibrate treatment reduced the necrotic area. LYSO-7 treatment enhanced PPARγ gene and protein expression in the stomach, and impaired local neutrophil influx and stasis of the microcirculatory network caused by Et/HCl administration. The effect seemed to be due to PPARγ agonist activity, as the LYSO-7 effect was abolished in GW9962 pre-treated mice. The reversal of microcirculatory stasis, but not neutrophil influx, was mediated by nitric oxide (NO), as L-NAME pre-treatment abolished the LYSO-7-mediated reestablishment of microcirculatory blood flow. This effect may depend on enhanced eNOS protein expression in injured gastric tissue. The pH and concentration of H(+) in the stomach were not modified by LYSO-7 treatment. In addition, LYSO-7 may induce less toxicity, as 28 days of oral treatment did not induce weight loss, as detected in pioglitazone treated mice. Thus, we show that LYSO-7 may be an effective treatment for gastric lesions by controlling neutrophil influx and microcirculatory blood flow mediated by NO

Arqueología industrial en Las Palmas de Gran Canaria durante la restauración: la arquitectura del pan

[ES] El presente artículo es el resumen de un estudio, investigación y búsqueda de la arquitectura industrial relacionada con el mundo de la panadería y molinería en el casco urbano de la capital grancanaria.

Lingue meno diffuse e corpora: studio empirico sulla terminologia amministrativa ladina

This study aims to the elaboration of juridical and administrative terminology in Ladin language, actually on the Ladin idiom spoken in Val Badia. The necessity of this study is strictly connected to the fact that in South Tyrol the Ladin language is not just safeguarded, but the editing of administrative and normative text is guaranteed by law. This means that there is a need for a unique terminology in order to support translators and editors of specialised texts. The starting point of this study are, on one side the need of a unique terminology, and on the other side the translation work done till now from the employees of the public administration in Ladin language. In order to document their efforts a corpus made up of digitalized administrative and normative documents was build. The first two chapters focuses on the state of the art of projects on terminology and corpus linguistics for lesser used languages. The information were collected thanks to the help of institutes, universities and researchers dealing with lesser used languages. The third chapter focuses on the development of administrative language in Ladin language and the fourth chapter focuses on the creation of the trilingual Italian – German – Ladin corpus made up of administrative and normative documents. The last chapter deals with the methodologies applied in order to elaborate the terminology entries in Ladin language though the use of the trilingual corpus. Starting from the terminology entry all steps are described, from term extraction, to the extraction of equivalents, contexts and definitions and of course also of the elaboration of translation proposals for not found equivalences. Finally the problems referring to the elaboration of terminology in Ladin language are illustrated.

Long-term dietary exposure to lead in young European children: comparing a pan-European approach with a national exposure assessment

Long-term dietary exposures to lead in young children were calculated by combining food consumption data of 11 European countries categorised using harmonised broad food categories with occurrence data on lead from different Member States (pan-European approach). The results of the assessment in children living in the Netherlands were compared with a long-term lead intake assessment in the same group using Dutch lead concentration data and linking the consumption and concentration data at the highest possible level of detail. Exposures obtained with the pan-European approach were higher than the national exposure calculations. For both assessments cereals contributed most to the exposure. The lower dietary exposure in the national study was due to the use of lower lead concentrations and...

Continuous geochemical monitoring by mass-spectometer in the Campi Flegrei geothermal area. An application at Pisciarelli-Solfatara (diffuse and fumarolic gases) and at the mud gases during drilling of the CFDDP pilot hole

During this work has been developed an innovative methodology for continuous and in situ gas monitoring (24/24 h) of fumarolic and soil diffusive emissions applied to the geothermal and volcanic area of Pisciarelli near Agnano inside the Campi Flegrei caldera (CFc). In literature there are only scattered and in discrete data of the geochemical gas composition of fumarole at Campi Flegrei; it is only since the early ’80 that exist a systematic record of fumaroles with discrete sampling at Solfatara (Bocca Grande and Bocca Nuova fumaroles) and since 1999, even at the degassing areas of Pisciarelli. This type of sampling has resulted in a time series of geochemical analysis with discontinuous periods of time set (in average 2-3 measurements per month) completely inadequate for the purposes of Civil Defence in such high volcanic risk and densely populated areas. For this purpose, and to remedy this lack of data, during this study was introduced a new methodology of continuous and in situ sampling able to continuously detect data related and from its soil diffusive degassing. Due to its high sampling density (about one measurement per minute therefore producing 1440 data daily) and numerous species detected (CO2, Ar, 36Ar, CH4, He, H2S, N2, O2) allowing a good statistic record and the reconstruction of the gas composition evolution of the investigated area. This methodology is based on continuous sampling of fumaroles gases and soil degassing using an extraction line, which after undergoing a series of condensation processes of the water vapour content - better described hereinafter - is analyzed through using a quadrupole mass spectrometer

Valutazione dei criteri ambientali minimi del pan gpp per i servizi di pulizia e loro applicazione al piano di riduzione degli impatti ambientali del presidio ospedaliero di Conegliano

The subject of my thesis is to develop an assessment of minimum environmental criteria of the Plan National Action of Green Public Procurement for the cleaning service in the Conegliano’s hospital, used as case of study, through the Life Cycle Assessment methodology, to improve the hospital cleaning service.