On-chip non-invasive and label-free cell discrimination by impedance spectroscopy

OBJECTIVES: Many flow-cytometric cell characterization methods require costly markers and colour reagents. We present here a novel device for cell discrimination based on impedance measurement of electrical cell properties in a microfluidic chip, without the need of extensive sample preparation steps and the requirement of labelling dyes. MATERIALS AND METHODS, RESULTS: We demonstrate that in-flow single cell measurements in our microchip allow for discrimination of various cell line types, such as undifferentiated mouse fibroblasts 3T3-L1 and adipocytes on the one hand, or human monocytes and in vitro differentiated dendritic cells and macrophages on the other hand. In addition, viability and apoptosis analyses were carried out successfully for Jurkat cell models. Studies on several species, including bacteria or fungi, demonstrate not only the capability to enumerate these cells, but also show that even other microbiological life cycle phases can be visualized. CONCLUSIONS: These results underline the potential of impedance spectroscopy flow cytometry as a valuable complement to other known cytometers and cell detection systems.

PROOF OF CONCEPT PROTOTYPE FOR A RAILROAD PEDESTRIAN WARNING SYSTEM USING WIRELESS SENSOR NETWORKS

Wireless sensor network is an emerging research topic due to its vast and ever-growing applications. Wireless sensor networks are made up of small nodes whose main goal is to monitor, compute and transmit data. The nodes are basically made up of low powered microcontrollers, wireless transceiver chips, sensors to monitor their environment and a power source. The applications of wireless sensor networks range from basic household applications, such as health monitoring, appliance control and security to military application, such as intruder detection. The wide spread application of wireless sensor networks has brought to light many research issues such as battery efficiency, unreliable routing protocols due to node failures, localization issues and security vulnerabilities. This report will describe the hardware development of a fault tolerant routing protocol for railroad pedestrian warning system. The protocol implemented is a peer to peer multi-hop TDMA based protocol for nodes arranged in a linear zigzag chain arrangement. The basic working of the protocol was derived from Wireless Architecture for Hard Real-Time Embedded Networks (WAHREN).

Primary pelvic pyomyositis in a neonate

Primary pyomyositis is a bacterial infection occurring in skeletal muscle with no obvious local or adjacent cause. It is classically an infection of the tropics, although it is reported in temperate climates with increasing frequency. Tropical pyomyositis occurs predominantly in children aged between 2 and 5 and in adults aged between 20 and 45 years, whereas most temperate pyomyositis cases occur in adults. Using a magnetic resonance imaging scan, we made the diagnosis of staphylococcal pelvic pyomyositis in a Swiss term-born infant with an initial working diagnosis of septic hip osteoarthritis.

Dalle sfide interculturali, al “romanzo”, al progetto socio educativo integrato

Intercultural challenge, “tale” and social educational strategy, Rome, November 2006: Police and Teachers deal differently with a little group of travellers. The analysis of this recent episode shows that intercultural issues are the basis of the educational commitment of the teachers but also of the prejudice of police and other people looking from outside to the relationship between teachers and travellers. The paradox of a double ethic concerning travellers needs to be deconstructed by the antiracist approach based on children rights. At the same time educational intervention cannot deal efficiently with emergency situations concerning children without cooperation with social work. The article underlines the role of democratic educational institutions in the developing appropriated strategies in dealing with complexes social issues which are not just to be “culturalised”.

Changing ‘Multi-Problem Families’ – Developing a Multi-Contextual Systemic Approach

Over the past 30 years the Marlborough Family Service in London has pioneered multi-family work with marginalized families presenting simultaneously with abuse and neglect, family violence, substance misuse, educational failure and mental illness. The approach is based on a systemic multi-contextual mode and this chapter describes the evolving work, including the establishment of the first permanent multiple family day setting, specifically designed for and solely dedicated to the work with seemingly ‘hopeless’ families. The ingredients of ‘therapeutic assessments’ of parents and families are outlined and the importance of initial network meetings with professionals and family members is emphasized.

Between Equity and Empathy: Social Professions and the New Accountability

This article explores the practical and ethical implications of the ‘new accountability’ (working to procedures, targets and standards) based on interviews with British social professionals. Although similar tendencies are present in other European countries, in Britain the rule-bound nature of social work is more intense. Practitioners who regard the ‘new accountability’ positively justify their views with reference to utilitarian and rights-based arguments relating to the promotion of good outcomes, the achievement of equity, respecting the consumer rights of service users and the rights of other stakeholders to information and value for money. Those practitioners who view the new accountability requirements negatively seem to speak in a different ‘moral voice’, which can be linked to more personal and situated approaches to ethics, stressing the importance of particular relationships in context, trust, sensitivity and a sense of ‘vocation’. Both ‘voices’ are part of professional practice, but the new accountability stresses the former at the expense of the latter. For social work to play the critical role identified by Walter Lorenz, maintaining a creative balance between equity and empathy will be important.

The Current Discussion on Men and Masculinities

In the past gender specific problems were mainly discussed in a female perspective. In the meantime there is a rising attentiveness in the living conditions of men and their coping strategies within critical life events. In this paper an appropriate frame of reference is outlined which can be used in those areas of social work where men are already discovered as a target group with special difficulties and needs.

Il razzismo della prosperità nell’Europa contemporanea - Riflessioni a margine del pensiero antirazzista di Walter Lorenz

Recent publishing on the migration phenomena in the communitarian and globalized Europe, puts in evidence a fundamental racism which is capable of making cultural processes grow and feed both chaos and social disorder. As a matter of fact we are approaching the ending debates on multicultural citizenship as well as on solidary integration and antiracism. Since the appearing of these phenomena, namely the huge post colonial migration in the nineteen-eighties, by which the colonized countries became almost “emigrant nurseries”, one could expect their stabilization. On the contrary, globalization and migration (twin subjects) everywhere still produce, at various levels, social disturbances together with some chauvinistic limitations as an ultimate kind of western prosperity defense. The peculiar European features of this new racism, less than ideological (superiority, homogeneity and civilizing mission), are confined to the concepts of patriotism, inequality and exclusion. In these terms one can understand why the new economic expansionism and the quest for new world markets makes European policies unstable, which remain undecided between conservatism, liberalism and extreme right. All this explains at least two things: the existing ambiguities of some European policies aiming to enhance particular forms of protectionism, and the difficulties in which the antiracist thought seems to be embedded. Indeed, according to what Walter Lorenz has already made clear, by using a well founded methodology, which prevents any fruitless protestations, it is impossible to contrast racism and nationalism. In such context, the educational field should try to use an operative epistemology. In other words the antiracist thought should dispose of competences and skills and, especially, personal and reflective capabilities. All this in order to avoid that which, in different historical scenes, permit the revival of the sense of moral opprobrium could not be identified with the political alibi to maintain privileges as well as advantages for the exclusive benefit of wealthy countries.

Europeanization Process in Italian Social Work Education

The article shows the process of Europeanization of the Italian social work education. After a brief excursus of the development of social work education in Italy, the paper presents the experiences made in the context of Socrates Erasmus project. Considering the results of the Thematic Network in Social Work organised by Parma University, some reflections are presented on the effects of Europeanization both respect the teachers and the students.

Social Work, Political Violence and Historical Change:

In this paper the author outlines the background to the history of the conflict in Northern Ireland which led to the current ‘Troubles’. In this discussion a range of key ideas are highlighted, including the nature of sectarianism and patterns of violence which have profoundly affected the society. The second part of the paper reviews a number of issues which face social workers when they try to deal with the effects of such violence as well as highlighting new challenges which have emerged as the society moves towards the resolution of conflict. It concludes with the argument that, wherever there is such conflict in the world, social workers need critically to understand the way in which political and social structures impinge upon their everyday practice.