Organizational, maintenance, repair parts, and special tools list for satellite communication terminals AN/MSC-64(V)1 (NSN 5895-01-112-5836), AN/MSC-64(V)2 (NSN 5895-01-088-2679), and AN/MSC-64(V)3 (NSN 5895-01-112-5838).

Includes index.

Operator's and organizational maintenance manual, aviation unit maintenance : countermeasures sets AN/ALQ-147A(V)1 and (V)2 : ((V)1-NSN-5865-01-077-6320) ((V)2-NSN-5865-01-077-6321).

"29 January 1980."

Operator and organizational maintenance manual including repair parts and special tool lists : test sets, telegraph, AN/GGM-15(V)1 (NSN 6625-00-464-1702), AN/GGM-15(V)2 (NSN 6625-00-442-6131).

Includes index.

Operators and organizational maintenance manual : test facility kit AN/GRM-95(V)2, (NSN 6625-01-104-8926).

Includes index.

Organizational maintenance repair parts and special tools lists for test sets AN/GGM-15(V)1 (NSN 6625-00-464-1702), AN/GGM-15(V)2 (NSN 6625-00-442-6131), and generator, signal ... analyzer, signal distortion ... oscilloscope ... dolly, test equipment V-434/GGM-15(V) (NSN 6625-00-435-7775).

Includes index.

General support maintenance repair parts and special tools list for test station, electronic equipment AN/USM-410(V)2 (NSN 6625-01-069-4223).

"18 October 1985."

Operator's and organizational maintenance manual : data processing set, AN/UYK-64(V)1 (NSN 7035-01-155-0153), AN/UYK-64(V)1x (NSN 7035-01-155-0154), AN/UYK-64(V)2 (NSN 7035-01-166-7855), AN/UYK-64(V)2x (NSN 7035-01-155-0155), AN/UYK-64(V)3 (NSN 7035-01-155-0156), AN/UYK-64(V)3x (NSN 7035-01-155-0157) ...

Includes index.

Organizational maintenance manual : multiplexer, digital TD-1337(V)1/G (NSN 7025-01-112-6311), multiplexer, digital TD-1337(V)2/G (NSN 7025-01-112-6310), multiplexer, digital TD-1337(V)3/G (NSN 7025-01-112-6312), multiplexer, digital TD-1337(V)4/G (NSN 7025-01-127-7020).

"November 1982."

Transportability guidance : ROLAND missile system, guided missile, intercept-aerial (less carrier), ROLAND fire units, (NSN 1425-01-146-9213 W/O NBC and NSN 1425-01-144-9802 W/NBC, AN/GSG-11(XO-1)(V)(2)), carrier, air defense (w/module loader mechanism) missile system, XM1058 truck transporter, (NSN 1450-01-134-9359, XM1058 (Lin C10788)), shop equipment ...

"16 September 1985."

Hand receipt covering contents of Components of End Item (COEI), Basic Issue Items (BII), and Additional Authorization List (AAL) for receiving set, radio, AN/TRQ-32(V), including AN/TRQ-32(V)1 NSN 5895-01-167-7655, AN/TRQ-32(V)2 NSN 5895-01-167-7655.

"November 1989."

Organizational maintenance repair parts and special tools list for navigational sets, TACAN, AN/ARN-103(V)1 (FSN 5826-00-167-1027) and AR/ARN-103(V)2 (NSN 5826-00-167-1026).

"11 August 1980."

Wills and inventories illustrative of the history, manners, language, statistics, etc. of the northern counties of England from the eleventh century downwards. [v. 2-4, Wills and inventories from the registry at Durham].

Mode of access: Internet.

The alpha(2)delta auxiliary subunit reduces affinity of omega-conotoxins for recombinant N-type (Ca(v)2.2) calcium channels

The omega-conotoxins from fish-hunting cone snails are potent inhibitors of voltage-gated calcium channels. The omega-conotoxins MVIIA and CVID are selective N-type calcium channel inhibitors with potential in the treatment of chronic pain. The beta and alpha(2)delta-1 auxiliary subunits influence the expression and characteristics of the alpha(1B) subunit of N-type channels and are differentially regulated in disease states, including pain. In this study, we examined the influence of these auxiliary subunits on the ability of the omega-conotoxins GVIA, MVIIA, CVID and analogues to inhibit peripheral and central forms of the rat N-type channels. Although the beta3 subunit had little influence on the on- and off-rates of omega-conotoxins, coexpression of alpha(2)delta with alpha(1B) significantly reduced on- rates and equilibrium inhibition at both the central and peripheral isoforms of the N-type channels. The alpha(2)delta also enhanced the selectivity of MVIIA, but not CVID, for the central isoform. Similar but less pronounced trends were also observed for N-type channels expressed in human embryonic kidney cells. The influence of alpha(2)delta was not affected by oocyte deglycosylation. The extent of recovery from the omega-conotoxin block was least for GVIA, intermediate for MVIIA, and almost complete for CVID. Application of a hyperpolarizing holding potential ( - 120 mV) did not significantly enhance the extent of CVID recovery. Interestingly, [R10K] MVIIA and [O10K] GVIA had greater recovery from the block, whereas [K10R] CVID had reduced recovery from the block, indicating that position 10 had an important influence on the extent of omega-conotoxin reversibility. Recovery from CVID block was reduced in the presence of alpha(2)delta in human embryonic kidney cells and in oocytes expressing alpha(1B-b). These results may have implications for the antinociceptive properties of omega-conotoxins, given that the alpha(2)delta subunit is up-regulated in certain pain states.

Overexpressed Ca-v beta 3 inhibits N-type (Ca(v)2.2) calcium channel currents through a hyperpolarizing shift of "ultra-slow" and "closed-state" inactivation

It has been shown that P auxiliary subunits increase current amplitude in voltage-dependent calcium channels. In this study, however, we found a hovel inhibitory effect of beta3 Subunit on macroscopic Ba2+ currents through recombinant N- and R-type calcium channels expressed in Xenopus oocytes. Overexpressed beta3 (12.5 ng/ cell cRNA) significantly suppressed N- and R-type, but not L-type, calcium channel currents at physiological holding potentials (HPs) of -60 and -80 mV At a HP of -80 mV, coinjection of various concentrations (0-12.5 ng) of the beta3 with Ca,.2.2alpha(1) and alpha(2)delta enhanced the maximum conductance of expressed channels at lower beta3 concentrations but at higher concentrations (>2.5 ng/cell) caused a marked inhibition. The beta3-induced Current suppression was reversed at a HP of - 120 mV, suggesting that the inhibition was voltage dependent. A high concentration of Ba-2divided by (40 mM) as a charge carrier also largely diminished the effect of P3 at -80 mV Therefore, experimental conditions (HP, divalent cation concentration, and P3 subunit concentration) approaching normal physiological conditions were critical to elucidate the full extent of this novel P3 effect. Steady-state inactivation curves revealed that N-type channels exhibited closed-state inactivation without P3, and that P3 caused an similar to40 mV negative shift of the inactivation, producing a second component with an inactivation midpoint of approximately -85 mV The inactivation of N-type channels in the presence of a high concentration (12.5 ng/cell) of P3 developed slowly and the time-dependent inactivation curve was best fit by the sum of two exponential functions with time constants of 14 s and 8.8 min at -80 mV Similar ultra-slow inactivation was observed for N-type channels Without P3. Thus, P3 can have a profound negative regulatory effect on N-type (and also R-type) calcium channels by Causing a hyperpolarizing shift of the inactivation without affecting ultra-slow and closed-state inactivation properties.

AllerTOP v.2 - a server for in silico prediction of allergens

Allergy is an overreaction by the immune system to a previously encountered, ordinarily harmless substance - typically proteins - resulting in skin rash, swelling of mucous membranes, sneezing or wheezing, or other abnormal conditions. The use of modified proteins is increasingly widespread: their presence in food, commercial products, such as washing powder, and medical therapeutics and diagnostics, makes predicting and identifying potential allergens a crucial societal issue. The prediction of allergens has been explored widely using bioinformatics, with many tools being developed in the last decade; many of these are freely available online. Here, we report a set of novel models for allergen prediction utilizing amino acid E-descriptors, auto- and cross-covariance transformation, and several machine learning methods for classification, including logistic regression (LR), decision tree (DT), naïve Bayes (NB), random forest (RF), multilayer perceptron (MLP) and k nearest neighbours (kNN). The best performing method was kNN with 85.3% accuracy at 5-fold cross-validation. The resulting model has been implemented in a revised version of the AllerTOP server (http://www.ddg-pharmfac.net/AllerTOP). © Springer-Verlag 2014.