Secret proceedings and debates of the convention assembled at Philadelphia, in the 1787, : for the purpose of forming the Constitution of the United States of America /

"Biographical sketch" [of Robert Yates]: p. [329]-335.

A popular cyclopedia of history, ancient and modern, forming a copious historical dictionary of celebrated institutions, persons, places, and things; with notices of the present state of the principal cities, countries, and kingdoms of the known world: to which is added, a chronological view of memorable events ...

An earlier edition appeared in 1835.

A text book on machine drawing for electrical engineers, forming an introductory treatise on the construction of electrical machinery and apparatus,

Mode of access: Internet.

Famous American men and women; a complete portrait gallery of celebrated people, whose names are prominent in the annals of the time, each portrait accompanied by an authentic biographical sketch, secured by personal interview--the whole forming a text book of national character.

Afterwards reissued under title: Eminent sons and daughters of Columbia.

Dictionary of geography : descriptive, physical, statistical, and historical, forming a complete gazetteer of the world /

Previous ed., 1862.

The new readings contained in Hermann's posthumous edition of Aeschylus translated and considered by George Burges. : forming an appendix to the prose translation. of Aeschylus.

Mode of access: Internet.

The imperial gazetteer of Scotland; or, Dictionary of Scottish topography, compiled from the most recent authorities, and forming a complete body of Scottish geography, physical, statistical, and historical.

Mode of access: Internet.

List of books forming the reference library in the reading room of the British museum.

Preface to 1st ed. (historical sketch) by J. Winter Jones, reprinted in v. 1, p. [viii]-xxvii.

A prospectus for forming a British colony on the island of New Caledonia : situated in the South Pacific Ocean, by a company, under royal charter, without causing expense to the British government.

Photocopy.

Chipless machining; methods of cold forming ferrous metals, including heading, rolling, spinning, swaging, extruding, and high-energy-rate forming.

Includes bibliography.

The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral

Reef-building corals contain fluorescent pigments termed pocilloporins that function by regulating the light environment of coral and acting as a photoprotectant in excessive sunlight. These pocilloporins are related to the monomeric green fluorescent protein and the tetrameric DsRed fluorescent proteins, which have widespread use as biotechnological tools. An intensely blue-coloured pocilloporin, termed Rtms5, was expressed in Escherichia coli, purified and crystallized. Rtms5 was shown to be tetrameric, with deep blue crystals that diffract to 2.2 Angstrom resolution and belong to space group I4(1)22. The colour of this pocilloporin was observed to be sensitive to pH and a yellow (pH 3.5) and a red form (pH 4.5) of Rtms5 were also crystallized. These crystals belong to space group P4(2)22 and diffract to 2.4 Angstrom resolution or better.

A pore-forming haemolysin from the hookworm, Ancylostoma caninum

Hookworms feed on blood, but the mechanism by which they lyse ingested erythrocytes is unknown. Here we show that Ancylostoma caninum, the common dog hookworm, expresses a detergent soluble, haemolytic factor. Activity was identified in both adult and larval stages, was heat-stable and unaffected by the addition of protease inhibitors, metal ions, chelators and reducing agents. Trypsin ablated lysis indicating that the haemolysin is a protein. A closely migrating doublet of hookworm proteins with apparent molecular weights of 60-65 kDa bound to the erythrocyte membrane after lysis of cells using both unlabeled and biotinylated detergent-solubilised hookworm extracts. In addition, separation of detergent-soluble parasite extracts using strong cation-exchange chromatography, resulted in purification of 60-65 kDa proteins with trypsin-sensitive haemolytic activity. Erythrocytes lysed with particulate, buffer-insoluble worm extracts were observed using scanning electron microscopy and appeared as red cell ghosts with approximately 100 nm diameter pores formed in the cell membranes. Red blood cell ghosts remained visible indicating that lysis was likely caused by pore formation and followed by osmotic disruption of the cell. (C) 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.