Development of an electrochemical maltose biosensor

In this work, electrochemical maltose biosensors based on mutants of the maltose binding protein (MBP) are developed. A ruthenium II complex (Ru II ), which is covalently attached to MBP, serves as an electrochemical reporter of MBP conformational changes. Biosensors were made through direct attachment of Ru II complex modified MBP to gold electrode surfaces. The responses of some individual mutants were evaluated using square wave voltammetry. A maltose-dependent change in Faradic current and capacitance was observed. It is therefore demonstrated that biosensors using generically this family of bacterial periplasmic binding proteins (bPBP) can be made lending themselves to facile biorecognition element preparation and low cost electrochemical transduction.

Estudio de accesibilidad a las zonas verdes urbanas mediante Sistemas de Información Geográfica

La creciente preocupación por parte de la sociedad respecto del medio ambiente que nos rodea, ha hecho que las administraciones de ámbito local recojan las inquietudes de los ciudadanos e intenten dar soluciones mediante la creación de lo que se viene a denominar espacios verdes urbanos: son nuestros parques y jardines públicos. Sin embargo, día a día surgen nuevas demandas por parte de los distintos grupos sociales, pasando de la mera creación o representación estética del campo en la ciudad a dotar a estos entornos de elementos en los que la practicidad toma un valor relevante. El objetivo del presente trabajo es servir como instrumento de información a las distintas administraciones en su labor de planificación de zonas verdes urbanas utilizando para ello Sistemas de Información Geográfica

Manuales técnicos para la elaboración de huertos de patio sostenible y transformación de la producción en San Jose de Cusmapa (Nicaragua)

El proyecto ha sido realizado dentro del Programa de Proyectos Fin de Carrera para el Desarrollo (PFCD), correspondiente a la IV Convocatoria. Se realizó en el marco del proyecto financiado por la Universidad Politécnica de Madrid,“Reducción de la vulnerabilidad alimentaria de las familias rurales de San José de Cusmapa”. Con el título de “Manuales técnicos para la elaboración dehuertos de patio sostenibles y trasformación de la producción”, elproyecto está formado por tres documentos, la memoria y sus anejos, presupuesto y manuales técnicos diseñados. Este proyecto no sólo tiene el carácter académico de Proyecto Fin de Carrera, sino también, educativo y humano. Es una herramienta para el desarrollo humano, que mediante la educación agronómica (uno de los objetivos específicos), trata de mejorar los índices de desarrollo (objetivo general). Durante el periodo de nueve meses(octubre 2010 a junio 2011) se realizó el trabajo de campo, mediante la recogida de información, aprendizaje de técnicas, ensayos, y otras experiencias vividas. Con los objetivos y el análisis de la situación actual se realizaron tres manuales técnicos, resultado final del proyecto. El primero,recoge las técnicas de elaboración de un huerto y los cultivos que se pueden sembrar. El segundo, está centrado en el control y prevención de plagas y enfermedades. El tercero se divide en dos bloques, el primero de conservación y trasformación de alimentos, de forma casera, y el segundo bloque la importancia de la dieta y la nutrición. Los manuales han sido diseñados para que estén al alcance de las personas que lo necesiten y de las cuales, muchas de ellas, han colaborado en su realización. El compromiso no solo es académico, sino también humano y emocional. El trabajo diario, los ensayos,los errores y aciertos, la convivencia con los beneficiarios y el aprendizaje continuo, me han permitido obtener una educación profesional y social, ayudándome a desarrollarme como persona.

The use of direct boundary element method for gaining insight into complex seismic site response

The boundary element method is specially well suited for the analysis of the seismic response of valleys of complicated topography and stratigraphy. In this paper the method’s capabilities are illustrated using as an example an irregularity stratified (test site) sedimentary basin that has been modelled using 2D discretization and the Direct Boundary Element Method (DBEM). Site models displaying different levels of complexity are used in practice. The multi-layered model’s seismic response shows generally good agreement with observed data amplification levels, fundamental frequencies and the high spatial variability. Still important features such as the location of high frequencies peaks are missing. Even 2D simplified models reveal important characteristics of the wave field that 1D modelling does not show up.

The direct boundary element method: 2D site effects assessment on laterally varying layered media (methodology)

The Direct Boundary Element Method (DBEM) is presented to solve the elastodynamic field equations in 2D, and a complete comprehensive implementation is given. The DBEM is a useful approach to obtain reliable numerical estimates of site effects on seismic ground motion due to irregular geological configurations, both of layering and topography. The method is based on the discretization of the classical Somigliana's elastodynamic representation equation which stems from the reciprocity theorem. This equation is given in terms of the Green's function which is the full-space harmonic steady-state fundamental solution. The formulation permits the treatment of viscoelastic media, therefore site models with intrinsic attenuation can be examined. By means of this approach, the calculation of 2D scattering of seismic waves, due to the incidence of P and SV waves on irregular topographical profiles is performed. Sites such as, canyons, mountains and valleys in irregular multilayered media are computed to test the technique. The obtained transfer functions show excellent agreement with already published results.

Mejorando el aprendizaje y la evaluación en e-learning usando recursos multimedia

Para mejorar los procesos de enseñanza, aprendizaje y evaluación a través de los sistemas de e-learning, proponemos la creación de nuevas actividades educativas basadas en el uso de recursos multimedia como el screencast. En este artículo explicaremos como el uso de estos recursos multimedia ha mejorado los procesos de aprendizaje y evaluación en el curso online "Seminarios Internaciones de Ciencia de Materiales I" que forman parte del proyecto ADA-Madrid. Nuestro propósito es estimular la creatividad, motivación y dinamismo del proceso de aprendizaje tanto para los profesores como para alumnos.

Influence of the rheological behaviour of the lubricant on the appearance of pitting in elastohydrodynamic eegime

This paper analyses the influence of lubricant behaviour on the appearance of pitting. It attempts to study the effect of viscosity?pressure relation, compressibility, film thickness?roughness relation and friction coefficient on pitting failure of the contacting elements.To explain these effects, we first deal with the influence of the oil on the lubrication of the contact using elastohydrodynamic theory and secondly two multiaxial fatigue criteria are used, Crossland criterion and Dang Van criterion, to evaluate the influence of the rheology on the appearance of pitting. Finally, different applications are presented together with a discussion on the results obtained.

New reynolds equation for line contact based on the carreau model modification by bair

This paper presents a new form of the one-dimensional Reynolds equation for lubricants whose rheological behaviour follows a modified Carreau rheological model proposed by Bair. The results of the shear stress and flow rate obtained through a new Reynolds–Carreau equation are shown and compared with the results obtained by other researchers.

Coronica de los señores Reyes de Castilla, Don Sancho el Deseado, Don Alonso el Octauo, y Don Enrique el Primero : en que se refiere todo lo sucedido en los Reynos de España, desde el año mil ciento y treinta y seis, hasta el de mil y docientos y diez y siete... / por Don Alonso Nuñez de Castro...

En port. grab. xil. esc real

The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray.

Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited mayor que50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.

Leyendas vascongadas / por José María de Goizueta.

Digitalización Vitoria-Gasteiz Archivos y Bibliotecas Julio 1994 18-64

TELMA: Technology enhanced learning environment for minimally invasive surgery

Background: Cognitive skills training for minimally invasive surgery has traditionally relied upon diverse tools, such as seminars or lectures. Web technologies for e-learning have been adopted to provide ubiquitous training and serve as structured repositories for the vast amount of laparoscopic video sources available. However, these technologies fail to offer such features as formative and summative evaluation, guided learning, or collaborative interaction between users. Methodology: The "TELMA" environment is presented as a new technology-enhanced learning platform that increases the user's experience using a four-pillared architecture: (1) an authoring tool for the creation of didactic contents; (2) a learning content and knowledge management system that incorporates a modular and scalable system to capture, catalogue, search, and retrieve multimedia content; (3) an evaluation module that provides learning feedback to users; and (4) a professional network for collaborative learning between users. Face validation of the environment and the authoring tool are presented. Results: Face validation of TELMA reveals the positive perception of surgeons regarding the implementation of TELMA and their willingness to use it as a cognitive skills training tool. Preliminary validation data also reflect the importance of providing an easy-to-use, functional authoring tool to create didactic content. Conclusion: The TELMA environment is currently installed and used at the Jesús Usón Minimally Invasive Surgery Centre and several other Spanish hospitals. Face validation results ascertain the acceptance and usefulness of this new minimally invasive surgery training environment.

Vida del venerable mártir el ilustrísimo señor D. Fr. Valentín de Berrio-Ochoa / por José Miguel de Arrieta Mascárua.

Digitalización Vitoria-Gasteiz Fundación Sancho el Sabio 2012

Comunica-Media: Uso de la grabación de clases, el screencast y la videoconferencia en el aula

A medida que la tecnología mejora y se extiende entre los usuarios es conveniente y necesario que los sistemas educativos se adapten y aprovechen lo que estas nuevas tecnologías pueden ofrecer. Así se puede motivar más a los alumnos y profesores ofreciéndoles contenidos más completos e interactivos. En esta comunicación se presenta el proyecto Comunica-Media del Servicio de Innovación Educativa de la Universidad Politécnica de Madrid (UPM). El objetivo principal de este proyecto es promover y evaluar el uso de la grabación de clases, la videoconferencia y el screencast en seis diferentes escuelas y asignaturas de la UPM.