Contemporary pollen flow, mating patterns and effective population size inferred from paternity analysis in a small fragmented population of the Neotropical tree Copaifera langsdorffii Desf. (Leguminosae-Caesalpinioideae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Parasitism of Greenbugs (Homoptera: Aphididae) by Lysiphlebus testaceipes (Hymenoptera: Braconidae) in Grain Sorghum: Implications for Augmentative Biological Control

Field cage studies were conducted to describe the relationship between the percentage of Lysiphlebus testaceipes (Cresson) parasitism (as measured by aphid mummies) and densities of greenbug, Schizaphis graminum Rondani, on grain sorghum, Sorghum bicolor L. In 1993 and 1994, a biotype E-susceptible grain sorghum hybrid was grown in field cages and L. testaceipes adults were released after each plant was infested with 20 biotype E greenbugs. The release rates were 0, 0.5, 1.0, and 2.0 wasps per plant in 1993, and 0, 0.16, 0.33, and 0.5 wasps per plant in 1994. Greenbugs and mummies were counted 1-2 times a week on all leaves of 2-4 randomly selected plants per cage. A release rate of 0.33-0.5 wasps per plant infested with 20 greenbugs maximized the number of mummies produced and prevented the greenbugs from reaching an economic threshold of 1,000 greenbugs per plant. Peak numbers of mummies occurred ≈400-500 DD (10°C base) after the initial wasp release. Regression analyses showed that the greenbug population started decreasing when the percentage of parasitism (as measured by mummies) reached 20-30 %. Greenbugs in the absence of wasps significantly reduced yield in 1994, but not in 1993.

Effect of atmosphere on the sintering and grain growth of tin oxide

High purity SnO 2 powder (>99.9%) was compacted in cylindrical pellets and sintered in atmospheres of dry argon, argon with water vapor, oxygen and CO 2 using 10 °C/min up to 1200 °C or isotherms in the range of 1000 to 1200 °C. Time, temperature and sintering atmosphere have large influence on grain growth and low influence on densification of this oxide. Surface diffusion is the dominant mechanism up to 1200 °C and evaporation-condensation is dominant above 1200 °C. The maximum linear shrinkage observed was about 2.0% and attributed to structural rearrangement of particles due to high capillary stresses developed with neighboring particles. © 1999 Trans Tech Publications.

Pollen viability in Syagrus romanzoffiana and S. coronata (Arecaceae)

Palms of the Syagrus genus have several natural hybrids in Brazil. They are widely cultivated around the world as ornamental palm. S. romanzqffiana is the parental of natural hybrids with other Syagrus species that are potentially useful as palm cultivated for heart of palm production. The objective of this work was to evaluate the pollen grains viability got from plants grown in the Experimental Nursery of the FCAVJ/UNESP, Jaboticabal, SP, Brazil. Pollen grains were sampled from inflorescence in three occasions: in the right day of anthesis, one day before and one day after anthesis. For each day, the inflorescence was subdivided in apical, medium and basal portions where pollen grains were collected. The results shown that: a) for S. coronata there was no statistical differences in pollen viability (pollen viability average = 92.89%); b) for S. romanzoffiana the average pollen viability was 87.27%, 87.47% e 86.85% before, during and after the anthesis, respectively. The basal portion pollen grains had lower viability (85.19% - p<0.01) than those from the medium (88.17%) and the apical (88.46%) portion of the inflorescence. These results shown that both species have no problems with pollen viability and can be used as parental of hybrids with other Syagrus species.

A load cell for grain-propelled ballistic rocket thrust measurement

A 1000-kgf resistive strain-gauge load cell has been developed for quality testing of rocket propellant grain. A 7075-T6 aluminum alloy has been used for the elastic column, in which 8 uniaxial, 120-Ω strain gauges have been bonded and connected to form a full Wheatstone bridge to detect the strain. The chosen geometry makes the transducer insensitive to moments and, also, to the temperature. Experimental tests using a universal testing machine to imposed compression force to the load cell have demonstrated that its behavior is linear, with sensitivity of 2.90 μV/kgf ± 0.34%, and negligible hysteresis. The designed force transducer response to a dynamic test has been comparable to that of a commercial load cell. © 2005 IEEE.

Path analysis and expected response in indirect selection for grain yield in soybean

This study aimed to determine the best auxiliary trait for indirect selection of soybean grain yield, through path analysis and in avoidance of the adverse effects of multicollinearity and expected response. Seventy-nine F5 soybean genotypes from the cross FT-Cometa x Bossier were used. The populations were distributed on the field was the families inserted with replicated controls. Primary and secondary traits of grain yield were evaluated in four phenotypically superior plants per family. The traits number of pods, height and number of nodes were considered as the most important, showing the best combination of direct effect and genotypic correlation. The number of pods achieved the highest expected gain through the estimation method based on the selection differential. On the other hand, plant height, by the method based on selection intensity, was not a good indicator of the most productive plants.

Evaluation of micronuclei frequency in Tradescantia pallida pollen mother cells treated with ethanolic extracts isolated from Cryptocarya mandioccana, Cryptocarya moschata and Pterogyne nitens

Although herbal extracts contain several classes of compounds with pharmacological activity, they also present toxic substances with mutagenic effects. The aim of the present study was to verify the mutagenicity of Cryptocarya moschata, Cryptocarya mandioccana and Pterogyne nitens using micronucleus assay in pollen mother cells (tetrads) in Tradescantia pallida (Trad-MCN). T. pallida inflorescences were treated with different concentrations of ethanolic extracts from the selected plant species. For C. mandioccana C. moschata and P. nitens, Trad-MCN assays were carried out simultaneoulsly, followed by positive control (formaldehyde 10000 ppm), negative control (Hoagland's solution), and vehicle control (Tween 20 20% or DMSO 3%). MCN present in tetrads were quantified in 300 tetrads/inflorescence and the mean (%) and standard error (SE) were established for at least 10 inflorescences per treatment. The extracts demonstrated dose response mutagenicity (clastogenic/aneugenic effects), respectively, C. mandioccana (0.5, 1.0 and 2.0 mg/mL) and P. nitens (1.0 and 2.0 mg/mL) However, no mutagenic effect was observed to C. moschata at the concentrations evaluated in the present study. We can conclude that the C. mandioccana and P. nitens extracts demonstrated clastogenic/aneugenic effects in highest concentrations whereas C. moschata extract did not demonstrate the same effect. © 2006 Sociedade Brasileira de Toxicologia.

The influence of grain size coating and shaft angulation of different diamond tips on dental cutting

Objectives: To evaluate the influence of the grain size coating and shaft angulation of ultrasonic and high-speed diamond burs on the dental cutting effectiveness. Materials and Methods: For the grain size evaluation, cavities were prepared on 40 incisors using high-speed (1092 and 1093F KG Sorensen®) and ultrasonic tips (8.2142 and 6.2142-CVDentus®). For the shaft angulation evaluation, cavities were prepared on 40 incisors using uniangulated (T1-CVDentus®) and biangulated (T1-A CVDentus®) ultrasonic tips. The cavities were bisected and examined at 50 magnification. The width and depth of cavities were measured by Leica QWin software. Kruskal-Wallis non-parametric test was used for analysis. Results: The grain size did not affect the cutting effectiveness, but the high-speed burs promoted deeper and wider cavities than the ultrasonic tips. The shaft angulation did not affect the cutting effectiveness; both the angulated and biangulated tips had greater cutting efficiency in dentin than in enamel. Conclusions: Ultrasonic tips promoted more conservative preparations and seemed promising for cavity preparation.

Comparison of Two Staining Methods for Pollen Viability Studies in Sugarcane

The present work aimed to compare two staining methods for pollen viability evaluation in sugarcane. Pollen from four sugarcane genotypes were collected at three different times (6.00, 8.00 and 9.00 a.m.) and tested for viability using two staining methods (iodine and lactophenol blue). Three anthers, of each genotype were crushed in a glass slide with a drop of the respective stain (iodine 0.1 N and lactophenol blue). The percentage of pollen viability was obtained with an optic microscope (250×) and compared with the pollen germination at culture media where one raquis of each genotype was gentle shaken in a petridish. Three replicates (petri dishes) was performed for each genotype which were maintained at the temperature of 25 °C and air humidity around 95 % for 30 min. The factors (staining methods, genotypes and times) and their interactions were evaluated by the analysis of variance, F test (P < 0.01) and the means compared by the t test (P < 0.05). The lactophenol blue staining was more sensible than the iodine staining method to detect the decrease of pollen viability which occurs naturally in sugarcane. The iodine staining method was more stable and easier than lactophenol to perform the inflorescence classification at any evaluated time (6.00, 8.00 and 9.00 a. m.). Both staining methods overestimated the viability obtained by the germination at culture media when performed at 6.00 a.m. © 2012 Society for Sugar Research & Promotion.

Intercropping time of corn and palisadegrass or guineagrass affecting grain yield and forage production

Intercropping corn (Zea mays L.) with forages, such as palisadegrass {Urochloa brizantha (Hochst. ex A. rich.) r. D. Webster [syn. Brachiaria brizantha (Hochst. ex A. rich.) Stapf]} or guineagrass [Megathyrsus maximus (Jacq.) B. K. Simon & S. W. L. Jacobs (syn. Panicum maximum Jacq.)], provides large amounts of biomass for use as straw in no-tillage systems or as pasture. However, it is important to evaluate what time these forages have to be sown into corn systems to avoid reductions in both corn and forage production. This study, conducted for three growing seasons at Botucatu, Brazil, evaluated nutrient concentration and yield of corn as affected by time of forage intercropped as well as forage's dry matter production. our data showed that intercropping systems did not reduce leaf nutrient concentrations and grain yield of corn in relation to sole corn. The simultaneous intercropping of corn and guineagrass resulted in the lowest plant population (51, 200 plant ha-1), number of ears per plant (1.0), and, consequently, the lowest corn grain yield (9801 kg ha-1). Guineagrass seeded at the time of corn fertilizer topdressing resulted in the highest plant population (59, 400 plants ha-1), number of ears per plant (1.2), and corn grain yield (12, 077 kg ha-1). Forage production was highest when intercrop was done simultaneously. palisadegrass could be intercropped with corn both simultaneously or at topdressing fertilization stage. In contrast, it is recommended that guineagrass should only be intercropped with corn at topdressingfertilization. © Crop Science Society of America.

Photoluminescence of core-shell nanoparticles made from yttrium stabilized zirconia powder grain coated with alumina

A YSZ@Al2O3 nanocomposite was obtained by Al 2O3 coating on the surface of yttrium stabilized zirconia via a polymeric precursor method. The resulting core-shell structures were characterized by X-ray diffraction, scanning electron microscopy, transmission electronic microscopy and PL spectra. The TEM micrographs clearly show a homogeneous Al2O3 shell around the ZrO2 core. The observed PL is related to surface-interface defects. Such novel technologies can, in principle, explore materials which are not available in the bulk single crystal form but their figure-of-merit is dramatically dependent on the surface-interface defect states. © 2013 This journal isThe Royal Society of Chemistry.

Production, purification, and characterization of a major penicillium glabrum xylanase using brewer's spent grain as substrate

In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+ and the reducing agents β-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+ as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost. © 2013 Adriana Knob et al.

Pollen morphology in Brazilian species of Codonanthe (Mart.) Hanst. and Nematanthus Schrader (Gesneriaceae)

Pollen morphology of 24 Brazilian species of Codonanthe and Nematanthus (Gesneriaceae) was investigated by means of light microscopy, scanning and transmission electron microscopy, in search of new characters that might contribute to taxonomic characterisation of the genera. The pollen grains are tricolpate or tricolporate, semitectate, reticulate, with simplicolumellate muri, sometimes with microreticulum and perforations, or duplicolumellate muri ring-shaped around lumina. The species studied were grouped into five pollen types on the basis of aperture characteristics and details of surface ornamentation. Sub-types were defined with reference to presence or absence of microreticulum and perforations around the lumina. Based on analysis of the morphology and measurements of pollen grains, we verified a close relationship among the genera, but can nevertheless discriminate their species. © 2013 Copyright Collegium Palynologicum Scandinavicum.

Sorghum grain yield, forage biomass production and revenue as affected by intercropping time

Sorghum is an excellent alternative to other grains in poor soil where corn does not develop very well, as well as in regions with warm and dry winters. Intercropping sorghum [Sorghum bicolor (L.) Moench] with forage crops, such as palisade grass [Brachiaria brizantha (Hochst. ex A. Rich) Stapf] or guinea grass (Panicum maximum Jacq.), provides large amounts of biomass for use as straw in no-tillage systems or as pasture. However, it is important to determine the appropriate time at which these forage crops have to be sown into sorghum systems to avoid reductions in both sorghum and forage production and to maximize the revenue of the cropping system. This study, conducted for three growing seasons at Botucatu in the State of São Paulo in Brazil, evaluated how nutrient concentration, yield components, sorghum grain yield, revenue, and forage crop dry matter production were affected by the timing of forage intercropping. The experimental design was a randomized complete block design. Intercropping systems were not found to cause reductions in the nutrient concentration in sorghum plants. The number of panicles per unit area of sorghum alone (133,600), intercropped sorghum and palisade grass (133,300) and intercropped sorghum and guinea grass (134,300) corresponded to sorghum grain yields of 5439, 5436 and 5566kgha-1, respectively. However, the number of panicles per unit area of intercropped sorghum and palisade grass (144,700) and intercropped sorghum and guinea grass (145,000) with topdressing of fertilizers for the sorghum resulted in the highest sorghum grain yields (6238 and 6127kgha-1 for intercropping with palisade grass and guinea grass, respectively). Forage production (8112, 10,972 and 13,193Mg ha-1 for the first, second and third cuts, respectively) was highest when sorghum and guinea grass were intercropped. The timing of intercropping is an important factor in sorghum grain yield and forage production. Palisade grass or guinea grass must be intercropped with sorghum with topdressing fertilization to achieve the highest sorghum grain yield, but this significantly reduces the forage production. Intercropping sorghum with guinea grass sown simultaneously yielded the highest revenue per ha (€ 1074.4), which was 2.4 times greater than the revenue achieved by sowing sorghum only. © 2013 Elsevier B.V.

Las bolsas de cereales y su influencia en las exportaciones de América Latina y el Caribe = The grain exchanges and their influence on Latin American and Caribbean exports

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