Desempenho do irrigâmetro no manejo da água de irrigação na cultura do feijoeiro

In this work, the aim was to evaluate the performance of Irrigameter in the management of the irrigation water, led in the culture of the bean plant, comparatively to the use of the methods standard of stove, tensiometers, Bouyoucos, automatic meteorological station and pan Class A. Irrigameter was adjusted to the soil characteristics, culture of the bean and irrigation equipment to confection the management ruler. For direct estimation of the evapotranspiration of the culture of the bean plant in your development stadiums, Irrigameter operated inside with heights of the levels of water of the evaporatorio same to 2, 3 and 5 cm, corresponding to the stadiums of initial development, vegetative development and flowering, respectively. The humidity obtained by the standard method of stove it was adopted as reference in the comparisons of the irrigation depth. Irrigameter can be used in the management of the irrigation to determine the consumption of water directly for a culture, in any development stadium; the methods that estimate the evapotranspiration of the culture overestimated the irrigation depth recommended by the standard method of stove, happening behavior contrary with the ones that determines the current humidity of the soil.

Desenvolvimento de "Nezara viridula" (L., 1758) (Hemiptera: Pentatomidae) em genótipos de soja

The soybean (Glycine max (L.)) has been significantly compromised by the attack of insect pests. The stink bug Nezara viridula (L.) (Hemiptera: Pentatomidae) is pointed as one of the most damaging species, reducing the quantity and quality of grain produced and requiring a large number of applications of insecticides in crops. Al-though chemical control is still the most widely used method of control, the use of resistant genotypes may represent a viable alternative in the management of this insect, reducing costs and impacts on the environment. This study evaluated the performance of nymphs of N. viridula in nine soybean genotypes under laboratory conditions (T = 25 + 2[degree]C, RH = 70 [plus or minus]10% and photoperiod = 12 h), aiming to detect possible resistance mechanisms. Thus, 25 nymphs/genotype were monitored daily by assessing the duration and mortality of the nymphal stage, the weight of nymphs and adults and the developmental period from egg to adult. The data revealed that the genotypes 'TMG-103', 'TMG-121, IAC-19', 'TMG-117', and 'IAC-24' caused 100% mortality of nymphs, indicating high levels of antibiosis and/or feeding non-preference expression. 'IAC-17' also prolonged the nymphal period, indicating the occurrence of resistance. The insects from 'PI-227687' showed the lowest average weight, also indicating the occurrence of antibiosis and/or feeding non-preference. Our results may be useful for soybean breeding programs focusing on development of genotypes resistant to insects.

Seed dispersal by fishes in tropical and temperate fresh waters: The growing evidence

Fruit-eating by fishes represents an ancient (perhaps Paleozoic) interaction increasingly regarded as important for seed dispersal (ichthyochory) in tropical and temperate ecosystems. Most of the more than 275 known frugivorous species belong to the mainly Neotropical Characiformes (pacus, piranhas) and Siluriformes (catfishes), but cypriniforms (carps, minnows) are more important in the Holarctic and Indomalayan regions. Frugivores are among the most abundant fishes in Neotropical floodplains where they eat the fruits of a wide variety of trees and shrubs. By consuming fruits, fishes gain access to rich sources of carbohydrates, lipids and proteins and act as either seed predators or seed dispersers. With their often high mobility, large size, and great longevity, fruit-eating fishes can play important roles as seed dispersers and exert strong influences on local plant-recruitment dynamics and regional biodiversity. Recent feeding experiments focused on seed traits after gut passage support the idea that fishes are major seed dispersers in floodplain and riparian forests. Overfishing, damming, deforestation and logging potentially diminish ichthyochory and require immediate attention to ameliorate their effects. Much exciting work remains in terms of fish and plant adaptations to ichthyochory, dispersal regimes involving fishes in different ecosystems, and increased use of nondestructive methods such as stomach lavage, stable isotopes, genetic analyses and radio transmitters to determine fish diets and movements. (C) 2011 Elsevier Masson SAS. All rights reserved.

Medicinal plants' hosting ability for nematode meloidogyne javanica and m. incognita

Baida, F.C., D.C. Santiago, L. H. I. Vidal, L. C. Baida, C. T. Stroze. 2011. Medicinal Plants' Hosting Ability for Nematode Suitability Meloidogyne incognita and M. javanica. Nematropica 41: 150-153.Medicinal plants can be attacked by pests, diseases and nematodes, which can compromise the quality and quantity of their healing properties and their yield. The aim of this study was to analyse the reaction of 15 medicinal plant species to the nematode Meloidogyne spp. The seedlings were produced by seed germination or cuttings under greenhouse conditions. A completely random experimental pattern of 15 treatments and 10 replications was chosen for the study. The seedlings were inoculated with approximately 5000 eggs + J(2)/plant 20 days after planting. Plant height and fresh and dry leaf weight, were measured 60 days after planting. The roots were collected, thoroughly washed and stained with Philoxine B and then processed to extract the eggs to determine the reproduction factor. For M. incognita the results showed that Chamomile was susceptible with RF = 1,64 making it a good host, and the other plants were resistant (RF < 1), and for M. javanica that all the plants showed resistance (RF < 1), Myrrh, Rue and Balsam demonstrating immunity (RF = 0).

Effect of different tannery sludge compost amendment rates on growth, biomass accumulation and yield responses of Capsicum plants

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cogeneration system design optimization

Cogeneration system design deals with several parameters in the synthesis phase, where not only a thermal cycle must be indicated but the general arrangement, type, capacity and number of machines need to be defined. This problem is not trivial because many parameters are considered as goals in the project. An optimization technique that considers costs and revenues, reliability, pollutant emissions and exergetic efficiency as goals to be reached in the synthesis phase of a cogeneration system design process is presented. A discussion of appropriated values and the results for a pulp and paper plant integration to a cogeneration system are shown in order to illustrate the proposed methodology.

Cogeneration system design optimization

Cogeneration system design deals with several parameters in the synthesis phase, where not only a thermal cycle must be indicated but the general arrangement, type, capacity and number of machines need to be defined. This problem is not trivial because many parameters are considered as goals in the project. An optimization technique that considers costs and revenues, reliability, pollutant emissions and exergetic efficiency as goals to be reached in the synthesis phase of a cogeneration system design process is presented. A discussion of appropriated values and the results for a pulp and paper plant integration to a cogeneration system are shown in order to illustrate the proposed methodology.

Distribution of Xylella fastidiosa in citrus rootstocks and transmission of citrus variegated chlorosis between sweet orange plants through natural root grafts

To study translocation of Xylella fastidiosa to citrus rootstocks, budsticks from citrus variegated chlorosis (CVC)-affected cv. Pera sweet orange (Citrus sinenesis (L.) Osb.) were top grafted on 15 citrus rootstocks. Disease symptoms were conspicuous 3 months later on all 15 rootstocks tested. The presence of X. fastidiosa was confirmed by light microscopy, double-antibody sandwich enzyme-linked immunosorbent assays, and polymerase chain reaction in rootlets and main roots of CVC-symptomatic Pera sweet orange in 11 of the 15 rootstocks tested. These results suggest that bacterial translocation from the aerial plant parts to the root system occurs but is not essential for X. fastidiosa to induce symptoms in the aerial parts. Bacterial translocation to the roots was not correlated with CVC leaf-symptom severity in the Pera scion. To determine if CVC disease could be transmitted by natural root grafts, two matched seedlings of each of four sweet orange cultivars (Pera, Natal, Valencia, and Caipira) were transplanted into single pots. One seedling rootstock of each pair was inoculated by top grafting with a CVC-contaminated budstick while the other seedling rootstock was cut but not graft inoculated. Transmission of X. fastidiosa from an inoculated plant to a noninoculated plant sharing the same pot was observed in all four sweet orange cultivars tested. Transmission was confirmed by observation of natural roots grafts between the two plants, presence of X. fastidiosa in the root grafts, and disease development in the uninoculated plants. This is the first report of transmission of CVC disease through natural root grafts.

Anti-ulcerogenic and analgesic activities of the leaves of Wilbrandia ebracteata in mice

Wilbrandia ebracteata (Cogn.) Cogn. is a medicinal plant belonging to the Cucurbitaceae family used popularly as an antiulcer and analgesic medicine. The hydromethanol extract of leaves was investigated to determine its anti-ulcerogenic (ethanol and indomethacin induced gastric damage) and analgesic (writhing and tail-flick tests) activities in mice (efficacy), its acute toxicity (safety), and its phytochemistry (quality control). Oral administration of leaf extract at a dose of 1000 mg/kg body wt. significantly reduced 73.3% of the total area of lesion in ethanol-induced gastric damage, but was inactive in an indomethacin-induced gastric damage test. The hydromethanol extract was also inactive in both analgesic tests. Oral administration of the leaf extract did not produce mortality in mice, while the LD50 value of the roots was 22.10 mg/kg body wt. in female mice and 58.31 mg/kg body wt. in male mice. Leaves of W. ebracteata reacted positively for steroids, flavonols, flavanones, saponins, tannins and xanthones and negative for other compounds, including cucurbitacins. Leaf extract of W. ebracteata was active as an anti-ulcerogenic, probably through increasing gastric defensive factors, and flavonoids might be the main constituent responsible for this activity.

Composition of coriander essential oil from Brazil

Coriander (Coriandrum sativum L.) is an annual and herbaceous plant, belonging to the Apiaceae family. Native of southern Europe and western Mediterranean region, this herb is cultivated world widely. This species, rich in linalool, has potential using as source of essential oil and as a medicinal plant. It has been used as analgesic, carminative, digestive, depurative, anti-rheumatic and antispasmodic agent. Its fruits (commonly called seeds) are used for flavoring candies, in cookery, perfumery, beverage and in tobacco industry. The aim of this study was to analyze the chemical composition of the seed essential oil of this species grown in Botucatu, São Paulo, Brazil. The experiment was carried out in Lageado Experimental Farm, Department of Plant Production, Agronomical Sciences College, São Paulo State University. The fruits were harvest 108 days after sowing. The essential oils were extracted by hydro distillation, in Clevenger apparatus. 50 g of fruits were used in each extraction. Three extractions were performed during three hours. The essential oils were analyzed in Gas Chromatography Mass Spectrometer (CG-MS, Shimadzu, QP-5000), equipped with DB-5 capillary column (30 m × 0,25 mm × 0,25 mm), split 1/20, injector for 240 C°, detector for 230 C°, dragged by gas He (1,7 mL/min), with programmed temperature for 40 C° (5 min)-150 C°, 4 C°/min; 150 C°-280 C°, 8 C°/min. The identification of the compounds was made by comparison of their spectra of masses with data from CG-MS (Nist 62 lib), literature references and retention index of Kovats. The 18 most important components were identified and quantified. The main components of the oil were linalool (77.48 %), γ-terpinene (4.64 %), α-pinene (3.97 %), limonene (1.28 %), geraniol (0.64 %) and 2-decenal (0.16 %).

Peroxidase enzyme (EC.1.11.1.7) activity as an indicator of water stress in sweet pepper plants

The present work was carried out at the Faculdade de Ciências Agronômicas - UNESP, Botucatu, SP. The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11.1.7) activity as an indicator of water stress in plants. Sweet pepper plants were grown for 230 days after transplanting of seedlings. The experiment was arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated, as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving the plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants.

Peroxidase activity as an indicator of water stress in sweet pepper plants

The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11. 1.7) activity as an indicator of water stress in plants. The experiment was carried out at the Faculdade de Ciências Agronômicas UNESP, Botucatu, SP. Sweet pepper plants were grown for 230 days after transplanting of seedlings and arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants.

Productivity of cherry tomatoes under protected cultivation carried out with different types of pruning and spacing

The aim of the study was to evaluate production and determine the level of total soluble solids for cherry tomatoes, under protected cultivation carried out with different types of spacing and pruning. The experiment was performed according to a randomized block design in a 2×2 factorial scheme, with two types of spacing between plants and two types of pruning, and with five repetitions. The cultivar 'Sindy' (De Ruiter) was utilized. Each experimental parcel contained seven plants, and fruits were collected from the five central plants. The seedlings were produced in Styrofoam trays of 128 cells and transplanted at 33 days after planting using two types of spacing between plants (0.3 and 0.5 m) and 1 m spacing between rows. The plants were grown as single-or double-stem form and staked individually. The parameters evaluated were the number of fruit per plant, fresh weight of fruit and the level of total soluble solids expressed in °Brix. There was no evidence of significant interaction between the treatments. For fresh weight of fruit per plant, there was a significant effect when the plants were grown with a spacing of 1 × 0.5 m, with 4.12 kg per plant, compared to a production of 3.00 kg per plant with a spacing of 1 × 0.3 m. With regard to the number of fruit per plant, a significant difference was seen between the two types of spacing, where a spacing of 1 × 0.3 m yielded a lower number of fruit per plant (188.8), compared to that observed with a spacing of 1 × 0.5 m (238.1). With regard to the two types of pruning, there was a significant effect for only the number of fruit per plant, where the mean number of fruit was 188.4 with one stem and 238.4 with two stems. No significant difference was observed between the treatments for the level of total soluble solids. It is concluded that for the cultivar 'Sindy', under protected cultivation, production is better with a spacing of 0.5 m between plants and the use of two stems per plant.

Selection of endogenous genes for gene expression studies in Eucalyptus under biotic (Puccinia psidii) and abiotic (acibenzolar-S-methyl) stresses using RT-qPCR

Background: Rust caused by Puccinia psidii Winter has been limiting for the establishment of new Eucalyptus plantations, as well as for resprouting of susceptible genetic materials. Identifying host genes involved in defense responses is important to elucidate resistance mechanisms. Reverse transcription-quantitative PCR is the most common method of mRNA quantitation for gene expression analysis. This method generally employs a reference gene as an internal control to normalize results. A good endogenous control transcript shows minimal variation due to experimental conditions. Findings. We analyzed the expression of 13 genes to identify transcripts with minimal variation in leaves of 60-day-old clonal seedlings of two Eucalyptus clones (rust-resistant and susceptible) subjected to biotic (P. psidii) and abiotic (acibenzolar-S-methyl, ASM) stresses. Conclusions. For tissue samples of clones that did not receive any stimulus, a combination of the eEF2 and EglDH genes was the best control for normalization. When pathogen-inoculated and uninoculated plant samples were compared, eEF2 and UBQ together were more appropriate as normalizers. In ASM-treated and untreated leaves of both clones, transcripts of the CYP and elF4B genes combined were the ones with minimal variation. Finally, when comparing expression in both clones for ASM-treated leaves, P. psidii-inoculated leaves, ASM-treated plus P. psidii-inoculated leaves, and their respective controls, the genes with the most stable expression were EgIDH and UBQ. The chitinase gene, which is highly expressed in studies on plant resistance to phytopathogens, was used to confirm variation in gene expression due to the treatments. © 2010 Laia et al; licensee BioMed Central Ltd.

The impact of fuse link manufacturers on arc flash energy

The traditional operational and hazard control paradigm of an electrical installation has several issues pertaining to the diversity of equipment. A large maintenance inventory is a reason for concern for any manager, but the arc flash hazard is a particularly new phenomenon and the effects of equipment diversity on this phenomenon are even newer. The class of arc hazard can be increased, simply by changing the fuse link or circuit breaker manufacturer. Management pressure to operate as well as non-standard practices and installations are also partly responsible. The aim of this study is to take a typical situation in an industrial plant and to statistically simulate, by means of a dedicated software program, the fuse arc flash interruption performance of various manufacturers. The purpose of this study is to obtain a more objective indication of the influence of different fuse link manufacturers on arc flash incident energy. © 2011 IEEE.