The role of native vegetation on infection rates of Calacarus heveae (Acari: Eriophyidae) by Hirsutella thompsonii (Ascomycota: Ophiocordycipitaceae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Honey bee lines selected for high propolis production also have superior hygienic behavior and increased honey and pollen stores

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Toxicidade de agroquímicos ao ácaro-da-leprose dos citros Brevipalpus phoenicis (Geijskes) e ao ácaro predador Neoseiulus californicus (McGregor) (Acari: Tenuipalpidae, Phytoseiidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Population dynamics of Aceodromus convolvuli (Acari: Mesostigmata: Blattisociidae) on spontaneous plants associated with Jatropha curcas in central Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Atividade in vivo de parasporina extraída de B. thuringiensis sobre o câncer colorretal murino (CT‐26)

Bacillus thuringiensis (Bt), is an environmental Gram-positive spore-forming bacterium that produces crystalline parasporal protein (Cry) during sporulation. The inclusions often exhibit strong and specific insecticidal activity, making Bt an agent for agricultural controlling insects pest, mites, protozoa and nematodes. Recent studies reported that some of these Crys do not show cytotoxicity against insects but they are capable to kill some human and animal cancer cells. These proteins were denominated parasporins (PS). However, antitumor activity of Bt parasporin on the development of murine colorectal cancer (CT-26), are not well studies and these are no reports on the in vivo effect of these proteins. Thus, the present study evaluated the in vitro and in vivo anti-tumoral activity of Bt parasporin against the murine colorectal cancer line CT-26. Therefore, Balb/c mice were s.c. inoculated with CT-26 cells and weekly treated with parasporin (i.p.) pre-activated by enzymatic digestion with trypsin or proteinase K. Our results have shown, for the first time, that despite the anti-tumor activity in vitro, parasporin crystals couldn’t combat tumor growth in vivo. Instead, this protein was highly toxic, affecting the liver and spleen, with possible effect on other organs, decreasing the survival of treated animals. The results indicate the need for studies to better detoxification or manipulation of parasporin for therapeutic use and new studies for analysis of toxicological effects of repetitive exposure of farmers to this toxin

Amostragem diurna versus noturna de ácaros (Arachnida: Acari) associados a Genipa americana L. (Rubiaceae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência de Cecropia pachystachya na incidência de ácaros (Arachnida, Acari) e de Leptopharsa heveae (Insecta, Hemiptera) em cultivo de seringueira

Cecropia pachystachya is a plant native to the states of Mato Grosso and São Paulo, usually found in rubber tree plantations. The goals of this study were to verify: a) whether the presence of C. pachystachya in rubber tree plantations influences the occurrence of mites in rubber trees; b) whether it can be used as a reservoir of predaceous mites; c) whether it serves as an alternative host for Leptopharsa heveae during the natural senescence of rubber trees. This study was conducted in two rubber tree plantations in São José do Rio Preto, in the state of São Paulo, Brazil. One of them had numerous naturally growing individuals of C. pachystachya, while the other was free of spontaneous plants. We registered high richness of predaceous mites on C. pachystachya, but only 37.5% of them were found on rubber trees, which reveals low displacement rate of mites between the plants. Among the species that were common to both plants, only the predaceous Zetzellia agistzellia and the phytophagous mites Allonychus brasiliensis and Eutetranychus banksi were influenced by the presence of C. pachystachya. The incidence of L. heveae did not differ between the plantations under study and, moreover, C. pachystachya was not used as an alternative host by this insect, since no individuals were registered on its leaves during the natural senescence of rubber trees.

Efeito Pesticida de Ésteres de Sacarose Sobre Calacarus heveae Feres (Acari: Eriophyidae) em Dois Clones de Seringueira

Sucrose esters are biodegradable, non pollutant and safe for health; moreover, they have shown great potential in the pest control. We tested a crude mix of sucrose esters on the survivorship and oviposition of Calacarus heveae Feres (Acari: Eriophyidae) females, an important rubber tree pest mite. The females were collected from rubber leaflets of the GT 1 and RRIM 600 clones. The mites were kept on stock arenas placed in rearing chamber at 28 + 0,1°C, 80 + 10% of relative humidity and 12h of light phase about two weeks before the assays. After this period, the mites were transferred to assay arenas made with leaflets from the same clone of stock arenas. The survivorship of females sprayed with sucrose esters in the concentrations of 1, 2, 3 and 4 g/L and their oviposition effect with 0.5 and 1 g/L were analyzed. The mites sprayed with 4 g/L had about 80% of mortality, while those with 1 g/L around 60%. All mites that died in the treatments with sucrose ester became dark with wrinkled tegument and decreased their body volume, suggesting dehydration. We also observed the efficacy of 1 g/L concentration to decrease the female oviposition in about 50%, in the third day after spraying. No differences were observed in the mortality and oviposition between females kept on both clones.

Distribuição espacial e determinação da depreciação quantitativa e qualitativa causada por Tetranychus ogmophallos (Acari: Tetranychidae) ao amendoinzeiro

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Distribuição espacial e temporal de Brevipalpus phoenicis (Acari: Tenuipalpidae) virulíferos e da leprose-dos-citros em pomares em controle químico

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Efeitos in vitro e in vivo do extrato de glândulas salivares de fêmeas de carrapatos Rhipicephalus sanguineus com 2 dias de alimentação sobre modelos tumorais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ação do extrato de glândulas salivares de fêmeas do carrapato Rhipicephalus sanguineus (Latreille, 1806) sobre a inibição da proliferação de células de linhagem tumoral

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Padrões de distribuição espacial e temporal em comunidades de ácaros em seringueira (Hevea SPP.) no Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ácaros associados a palmeiras na Amazônia, com ênfase nos Phytoseiidae (Acari: Mesostigmata)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Diversidade de ácaros em diferentes culturas e em plantas da vegetação natural do Peru, com ênfase nos Phytoseiidae (Mesostigmata)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV