Fisheries research in Uganda

Evolution of fisheries research and FIRRI The earliest approach to fisheries research in Uganda dates from the first fisheries survey of Lake Victoria by Michael Graham between 1927 and 1928 (Graham, 1929). Based on references to the rich fisheries that were reported to Graham, it appears that during the 18th Century, catch per net per night averaged 300 tilapia, a revelation that led Graham to conclude that Lake Victoria is a tilapia lake. The "tilapia" later came to be known as Tilapia esculenta and T variabilis (Oreochromis esculentus and O. variabilis) respectively.

Geographic and economic setting of Lake Victoria

Lake Victoria, besides being the second largest in the world after Lake Superior, is the largest tropical lake. Its waters are shared by Kenya (6% of the surface area), Uganda (43%), and Tanzania (51%). Before dramatic structural and functional changes manifested in the lake's ecosystem especially in the 1980s, fish life flourished in the lake's entire water column at all times of the year. Currently, the situation is much more different from what it was in the past. The exponential increase in the introduced Nile perch (Lates niloticus) and Nile tilapia (Oreochromis niloticus) stocks, siltation, wetland degradation and eutrophication have characterised the lake ecosystem. The two exotic species and the small native cyprinid (Rastrineobola argentea) form the basis of the commercial fishery that was once dominated by two native tilapiines (Oreochromis esculentus and Oreochromis variabilis) and five other large-bodied endemic fishes. Severe deoxygenation observed at shallow depths (Ochumba 1990; Hecky et al., 1994) indicates that a large volume of the lake is unable to sustain fish life. The Lake Victoria catchment is one of the most densely populated areas in East Africa, encompassing a population of about 30 million people. Widespread poverty resulting from high inflation rates, lack of opportunities and general unemployment have characterised the lakeside communities over much of the last two decades. The biophysical environment in which Lake Victoria exists makes the lake particularly susceptible to changes that occur as a result of human modification to the watershed or the lake itself, thus rendering benefits from the lake unsustainable.

The history of fish communities, biodiversity and environment of Lake Victoria and the lessons learnt

The first fishery survey of Lake Victoria was conducted between 1927 and 1928 (Graham 1929). Atthat time, the lake had a diverse fish fauna and the fishery was dominated by two endemic tilapiine cichlids; Oreochromis esculentus (Graham 1929) and O. variabilis (Boulenger 1906). There were a number of other species such as Protopterus aethiopicus Heckel 1851, Bagrus docmac (Forsk.) 1775, Clarias gariepinus (Burchell), Barbus species, mormyrids, Synodontis spp, Schilbe intermedius (Linn.) 1762 and Rastrineobola argentea Pellegrin, 1904 that were also abundant in the lake most of which made a significant contribution to the fishery (Graham 1929, Worthington 1929, 1932, Kudhongania & Cordone 1974). Haplochromine cichlids were represented by at least 300 species more than 99% of them endemic (Greenwood, 1974; Witte et al., 1992 a & b). The fishery of Lake Victoria was similar to that of lakes Kyoga and Nabugabo (Worthington 1929; Trewavas 1933; Greenwood 1965, 1966; Beadle 1962, 1981). There were also important fisheries on the inflowing rivers of Lake Victoria, the most important of which were Labeo victorianus and Barbus altianalis (Cadwallader 1965). The small sized species notably Rastrineobola argentea and haplochromines cichlids were not originally commercially exploited.

Evolution of the tilapia fishery with specific reference to the Nile tilapia (Oreochromis niloticus Linne)

As a fishery, the immensely large (c. 68,800 km2 ) Lake Victoria is a unique ecosystem which together with a riverine connection to the Lake Kyoga basin share a common endemic "Victorian" fish fauna (Greenwood 1966). Until the 1950s, the single socio economically most important species of fish in these two lakes was the native Oreochromis esculentus Graham (Graham 1929) even though the lake also contained a second native tilapiine, 0reochromis variabilis , and over 300 other fish species (Beauchamp, 1956).

Impacts of fishing gears, fishing methods and fishing effort in the fisheries of Lake Victoria and proposals for management

The initial subsistence fisheries of Lake Victoria were dominated by two indigenous tilapiines, Oreochromis esculentus (Graham 1929) and Oreochromis variabilis Boulenger 1906, exploited with simple fishing crafts and gears that had little impact on the fish stocks (Jackson 1971). Commercial fisheries, targeting the tilapia fishery, started at the beginning of the 20th Centurywhen cotton flax gillnets were first introduced in 1905 into the Nyanza Gulf in Kenya. Gillnets were quickly adopted around the whole lake and consequently, the native methods of fishing soon died out (Jackson 1971). Following the introduction of gillnets, fishing boats and their propulsion methods were also improved. These improvements in fishing capacity coincided with development of urban centres and increasing human population around the lake, which increased the demand for fishery products. To satisfy the increasing demand, fishing effort increased greatly during the 20th century, despite the decline of catch per unit of effort (CPUE) (Jackson 1971; Ogutu-Ohwayo 1990). The initial catch rates of 127mm (5 inch) mesh size gill nets in the tilapia-based fishery, in 1905, was in the range of 50 to 100 fish per gillnet of approximately 50 m in length. However, twenty years later, the catch rates of gillnets of the same mesh size had declined to about six fish per net and gillnets of smaller mesh sizes, which had better catch rates, had been introduced suggesting overfishing (Worthington and Worthington, 1933).

Fish species diversity in the Victoria and Kyoga lake basins: their conservation and sustainable use

Introduction of exotic fish species especially the Nile perch Lates niloticus, is believed to be responsible for the decline of fish species diversity in lakes Victoria, Kyoga and Nabugabo.About 60% of the haplochromine cichlids are thought to have become extinct from L. Victoria due to predation by the Nile perch. However there are many lakes satelite to the lakes Victoria and Kyoga basins which still have fish fauna similar to that of the main lakes. many of the satellite lakes are separated from the main lakes in, which Nile perch was introduced by extensive swamps that provide a barrier to Nile perch .A survey was carried out in a number of these satelite lakes and an inventory made of existing fish species. Their distribution and relative abundances were also determined. The lakes studied included Nawampasa, Nakuwa,Kawi Lamwa Gigate, Nyaguo, Agu, Nabugabo. Kayanja, Kaytigi, Mburo, Kachera and Wamala.Some habitats within the main lakes Victoria and Kyoga, especially those with rocky outcrops· and macrophyte cover that provide refugia for endangered species from Nile perch,were also surveyed) Various stations along the River Nile were also sampled to quantify the fish species that are still resent. Kyoga minor lakes were found to have the highest number of fish species especially of haplochromine cichlids. Many haplochromine trophic groups that were thought to be extinct from 1. Victoria still occur in these lakes.!Some of the satellite lakes, especially lakes Kayugi, Mburo and Kachera still contain .healili populations of oreochromis. I esculentus that could be used as brood stock in fish farming. Many of these lakes should .I ( I therefore be protected for conservation offish species diversity

Trophic interrelationships and food-webs among the fishes in ecosystems of the Victoria and Kyoga lake basin

The Victoria and Kyoga lake basins had a high fish species diversity with many fish species that were found only in these lakes. Two Tilapiines species Oreochromis esculentus and Oreochromis variabilis were the most important commercial species in these lakes and were found nowhere else on earth except in the Victoria and Kyoga lake basins (Graham 1929, Worthington 1929). Lakes Kyoga and Nabugabo also had endemic haplochromine species (Worthington 1929, Trewavas 1933, Greenwood 1965, 1966). As stocks of introduced species increased, stocks of most of the native species declined rapidly or disappeared altogether. The study was carried out on Lakes Victoria and Kyoga, River Nile, some selected satellite lakes from the two basins namely Lakes Mburo, Kachera, Wamala, Kayanja, Kayugi, Nabugabo, Victoria, Victoria nile and River Sio(Victoria lake basin). Lakes Kyoga (Iyingo), Nawampasa, Nakuwa, Gigati, Nyaguo, Agu, Kawi and Lemwa (Kyoga lake basin). Species composillon and relative abundance of fishes were estimated by detennining the overall average total number of each species encountered. A trophic consists of species using the same food category. Shannon-Weaver Index of diversity H (Pielou, 1969) and number of trophic groups, were used to estimate the Trophic diversity of various fish species in the lakes. Food analysis has been done on some fishes in some of the sampled lakes and is still going on, on remaining fishes and in some lakes. Generally fish ingested detritus, Spirulina, Melosira, filamentous algae, Planktolyngbya, Microcysists, Anabaena, Merismopedia, Spirogyra, higher plant material, rotifers, Ostracodes, Chironomid larvae and pupae, Choaborus larvae, Odonata, Povilla, Insect remains, Caridina, fish eggs and fish. Eight trophic groups were identified from thes food items ingestes. These included detritivores, algae eaters, higher plant eaters, zooplanktivores, insectivores, molluscivores, prawn eaters, paedophages and piscivores. Trophic diversity by number of trophic groups was highest in Lake Kyoga (6) followed by lakes Kayugi, Nabugabo, River Nile and Mburo (3) and the lowest number was recorded in kachera (2).

The impact of fishing gears and methods: with recommendations for management of the fisheries of Lakes Victoria, Kyoga and Nabugabo

Historical analysis has shown that use of destructive fishing gears and methods contributed much to the initial depletion of fish stocks from Lakes Victoria and Kyoga. From about 1930 to 1960, the fisheries of Lake Victoria were managed by controlling the mesh size of gill nets. Gill net s of less than 127 mm (5) stretched mesh had been prohibited on Lake Victoria because they cropped immature Oreochromis esculentus (Ngege) which were at that time the most important commercial species. When the mesh size restriction was repealed in the Ugandan, Tanzanian and Kenya, there was a shift to smaller meshes which cropped immature tilapia and other large species and led to a collapse in the fishery.

Some characteristics of the fish fauna of a dam in the Lake Victoria region of Tanzania including the effects of multispecific stocking with tilapia species

Malya dam had been stocked with several species of Tilapia. However all but the species endemic to the region, T esculentus, made only marginal contributions to the fishery. T. zilli particularly had failed to establish itself, probably because its niche had been previously filled by T. rendalii. Planktivorou Tilapia species predominate in Malya dam but in smaller dams in the region herbivoorous tilapia are relatively more abundant. Six non-cichlid species and one of Haplochromis were found in the dam. Tilapia esculenta the most economically important species, was estimated to grow within 9-10 cm first year and 16-18 cm second year. The largest, specimen caught was 30.5 cm and the smallest mature individual 19 cm. The fish of the dam grew more slowly and matured at a smaller size than those of the same species in Lake Victoria The biological pattern of T. esculenta in Malya dam was similar to that of this species in Lake Victoria.

Los camarones comerciales de la familia Penaeidae de la costa atlántica de América del Sur. Clave para el reconocimiento de las especies y datos bioecológicos

Este estudio está orientado a identificar correctamente las especies comerciales, evitar los errores que surgen como consecuencia de confundir las especies en los muestreos utilizador en los trabajos de bioestadística y por ende en la estimación del efectivo de las poblaciones. Los peneidos comerciales del litoral Atlántico de Sudamérica son fáciles de reconocer, con excepción de los representantes del género Penaeus, que en algunos casos puede ofrecer ciertas dificultades debido a la similitud en la morfología externa. Se espera que este estudio sea una contribución útil a biólogos pesqueros, pescadores y técnicos que tienen una actividad relacionada con la pesca. Hasta el presente existen sólo siete especies de camarones de la familia Penaeidae de valor comercial en aguas de Colombia, Guayanas, Venezuela, Brasil, Uruguay y Argentina.

A note on recent advances in the genetic characterization of tilapia stocks in Lake Victoria region

Oreochromis esculenta, the original "ngege" is virtually extinct in Lake Victoria, and is limited to satellite lakes and reservoirs in the greater Lake Victoria region. Oreochromis variabilis can still be found in Lake Victoria and some satellite lakes in the Kyoga System, but in small numbers and only at a few localities (WANDERA and KAUFMAN, unpub. data). Little is known about the influence that species translocations have had on the genetic structure of these crucial fishery species, and even the source of the parent stocks for the introductions remain obscure. Genetic variability was examined within and among allopatric populations of three species in the tilapiine genus Oreochromis: O. esculentus (endemic to Lakes Victoria and Kyoga), and two exotic species introduced to Lake Victoria in the late 1950's to supplement the failing fisheries for native tilapiines, O. niloticus and O. leucostictus.

原位杂交研究对虾白斑杆状病毒在虾体内感染过程

应用地高辛标记的对虾白斑杆状病毒 (whitespotsyndromebaculovirus,WSSV)核酸探针 ,与人工感染后不同时间采集的对虾组织样品进行原位杂交 ,以动态研究病毒从侵染至对虾发病死亡的过程。将典型感染WSSV的病虾组织投喂健康对虾 ,结果显示 :WSSV首先通过侵染消化道上皮进入虾体内增殖 ,此后随着细胞裂解、病毒粒子释放 ,游离的病毒粒子伴随血淋巴循环进而侵染其它靶组织 ,直至对虾发病死亡。

Phylogenetic relationships among the genera of the Penaeidae (Crustacea : Decapoda) revealed by mitochondrial 16S rRNA gene sequences

The phylogenetic relationships within the family Penaeidae are examined based on mitochondrial 16S rRNA gene sequence analysis of 30 species from 20 genera. The analysis generally supports the three- tribe scheme proposed by Burkenroad ( 1983) but it is not consistent with the five- group classification of Kubo ( 1949). Three clades are resolved: ( Penaeus sensu stricto + Fenneropenaeus + Litopenaeus + Farfantepenaeus + Marsupenaeus + Melicertus + Funchalia + Heteropenaeus), ( Metapenaeus + Parapenaeopsis + Xiphopenaeus + Rimapenaeus + Megokris + Trachysalambria) and ( Metapenaeopsis + Penaeopsis + Parapenaeus), corresponding to the Penaeini, Trachypenaeini and Parapenaeini respectively, while the affinities of Atypopenaeus and Trachypenaeopsis are obscure. The molecular data support that Miyadiella represents the juvenile stage of Atypopenaeus. Within the Trachypenaeini, Trachypenaeus sensu lato is clearly paraphyletic, while the monophyly of Penaeus sensu lato in the Penaeini is questionable.

A preliminary phylogenetic analysis of Metapenaeopsis (Decapoda : Penaeidae) based on mitochondrial dna sequences of selected species from the Indo-West Pacific

Phylogenetic relationships within Metapenaeopsis remain largely unknown. The modern revision of the genus suggests that the shape of the petasma, followed by the presence of a stidulating organ, are the most important distinguishing taxonomic features. In the present study, phylogenetic relationships were studied among seven Metapenaeopsis species from the Indo-West Pacific based on partial sequences of mitochondrial 16S rRNA and cytochrome c oxidase I (COI) genes. Mean sequence divergence was 6.4% for 16S and 15.8% for COI. A strikingly large nucleotide distance (10.0% for 16S and 16.9% for COI) was recorded between M. commensalis, the only Indo-West Pacific species with a one-valved petasma, and the other species with a two-valved petasma. Phylogenetic analyses using neighbor-joining, maximum parsimony, and maximum likelihood generated mostly identical tree topologies in which M. commensalis is distantly related to the other species. Two clades were resolved for the remaining species, one with and the other without a stridulating organ, supporting the main groupings of the recent taxonomic revision. Results of the present study also indicate that the deep-water forms represent a relatively recent radiation in Metapenaeopsis.