Report on the Iowa Department of Human Rights for the year ended June 30, 2012

Report on the Iowa Department of Human Rights for the year ended June 30, 2012

Report on the Iowa Civil Rights Commission for the year ended June 30, 2012

Report on the Iowa Civil Rights Commission for the year ended June 30, 2012

MHC-genotype of progeny influenced by parental infection.

In a previous series of in vitro fertilization experiments with mice we found non-random combination of major histocompatibility complex (MHC) haplotypes in the very early embryos. Our results suggested that two selection mechanisms were operating: (i) the eggs selected specific sperm; and (ii) the second meiotic division in the eggs was influenced by the type of sperm that entered the egg. Furthermore, the proportion of MHC-heterozygous embryos varied over time, suggesting that non-random fertilization was dependent on an external factor that changed over time. As a higher frequency of heterozygous individuals correlated with an uncontrolled epidemic by MHV (mouse hepatitis virus), we suggested that MHV-infection might have influenced the outcome of fertilization. Here, we present an experiment that tests this hypothesis. We infected randomly chosen mice with MHV and sham-infected control mice five days before pairing. We recovered the two-cell embryos from the oviduct, cultured them until the blastocyst stage, and determined the genotype of each resulting blastocyst by polymerase chain reaction. We found the pattern that we expected from our previous experiments: virus-infected mice produced more MHC-heterozygous embryos than sham-infected ones. This suggests that parents are able to promote specific combinations of MHC-haplotypes during fertilization according to the presence or absence of a viral infection.

Youth Rights and Responsibilities Handbook. 2004

This handbook is designed to provide you with general information regarding your rights and the effect and operation of the law upon you as a minor. It is not intended to be legal advice. Should you find yourself in a situation with a specific legal problem, you should obtain competent legal advice from attorney

Human Rights Performance Report, FY2011

Agency Performance Report

Human Rights Performance Report, FY2011

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2004

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2005

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2006

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2007

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2008

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2009

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2010

Agency Performance Report

Iowa Civil Rights Commission Performance Report, FY 2011

Agency Performance Report

MHC class I expression dependent on bacterial infection and parental factors in whitefish embryos (Salmonidae).

Ecological conditions can influence not only the expression of a phenotype, but also the heritability of a trait. As such, heritable variation for a trait needs to be studied across environments. We have investigated how pathogen challenge affects the expression of MHC genes in embryos of the lake whitefish Coregonus palaea. In order to experimentally separate paternal (i.e. genetic) from maternal and environmental effects, and determine whether and how stress affects the heritable variation for MHC expression, embryos were produced in full-factorial in vitro fertilizations, reared singly, and exposed at 208 degree days (late-eyed stage) to either one of two strains of Pseudomonas fluorescens that differ in their virulence characteristics (one increased mortality, while both delayed hatching time). Gene expression was assessed 48 h postinoculation, and virulence effects of the bacterial infection were monitored until hatching. We found no evidence of MHC class II expression at this stage of development. MHC class I expression was markedly down-regulated in reaction to both pseudomonads. While MHC expression could not be linked to embryo survival, the less the gene was expressed, the earlier the embryos hatched within each treatment group, possibly due to trade-offs between immune function and developmental rate or further factors that affect both hatching timing and MHC expression. We found significant additive genetic variance for MHC class I expression in some treatments. That is, changes in pathogen pressures could induce rapid evolution in MHC class I expression. However, we found no additive genetic variance in reaction norms in our study population.