Comparison of two methods of synchronization of estrus in brown brocket deer (Mazama gouazoubira)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seasonal variation in growth and yield of the brown mussel Perna perna (L.) cultured in Ubatuba, Brazil

Seasonal variation in growth and yield of cultured brown mussels Perna perna was studied in Ubatuba, south-east Brazilian coastline. Young mussels were transplanted (seeded) into four groups of 16 polyethylene net tubes 2 m long, suspended from a wooden raft (18 m 2). The first group was set out in April (autumn), the next in July (winter), the next in October (spring) 1984 and the final group in January (summer) 1985. One net tube of each group was sampled monthly and biometric data were collected. Growth was initially faster for the spring group, but at the end of the culture period length and weight were not statistically different between groups. L(∞) and W(∞) were 73.9, 71.3, 72.7 and 73.8 mm and 26.3, 23.9, 25.7 and 25.7 g for the autumn, winter, spring and summer groups, respectively. Maximum yield was attained 9 months after seeding for groups of autumn, winter and spring (7.2, 5.2 and 6.3 kg x m -1, respectively) and after 10 months for the summer group (6.9 kg X m -1). The conclusion of this study is that, growth and yield were unaffected by the season of seeding at the study site and that it is not commercially worthwhile to farm mussels more than 9 months, due to yield decrease.

Continuous emission versus freeze-out via hanbury brown-twiss

The effect of the continuous emission hypothesis on the two-pion Bose-Einstein correlation function is discussed and compared with the corresponding results based on the usual freeze-out. Sizable differences in the correlation function appear in these different descriptions of the decoupling process. This means that, when extracting properties of the hot matter formed in high-energy heavy-ion collisions from the data, completely different conclusions may be reached according to the description of the particle emission process adopted.

Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus

Postbloom fruit drop (PFD) of citrus, caused by Colletotrichum acutatum, produces orange-brown lesions on petals and results in premature fruit drop and the retention of calyces. C. gloeosporioides is common in groves and causes postharvest anthracnose on fruit. Both diseases are controlled effectively by the fungicide benomyl in research fields and commercial orchards. Highly sensitive and resistant isolates of C. gloeosporioides were found, whereas all isolates of C. acutatum tested were moderately resistant. In preliminary studies conducted in vitro with three isolates of each, mycelial growth of sensitive isolates of C. gloeosporioides was inhibited completely by benomyl (Benlate 50 WP) at 1.0 μg/ml, whereas resistant isolates grew well at 10 μg/ml. Growth of all isolates of C. acutatum was inhibited by about 55% at 0.1 μg/ml and by 80% at 1.0 μg/ml. Spore germination of C. acutatum was inhibited more at 0.1 μg/ml than at 1.0 μg/ml or higher concentrations. In all, 20 isolates of C. acutatum from 17 groves and 20 isolates of C. gloeosporioides from 7 groves were collected from locations with different histories of benomyl usage in São Paulo, Brazil, and Florida, United States. Benomyl at 1.0 μ.g/ml completely inhibited growth of 133 isolates of C. gloeosporioides, with the exception of 7 isolates that were highly resistant to the fungicide, whereas all isolates of C. acutatum were only partially inhibited at 0.1 and 1.0 μg/ml. Analysis of variance indicated that the sensitivity of the isolates of C. acutatum was not affected by benomyl usage or grove of origin, and country of origin had only minor effects. No highly resistant or sensitive isolate of C. acutatum was recovered. Partial sequencing of the β-tubulin gene did not reveal nucleotide substitutions in codons 198 or 200 in C. acutatum that usually are associated with benomyl resistance in other fungi.

Postharvest conservation of 'Tommy Atkins' mango fruit influenced by gamma radiation, wax, hot water, and refrigeration

It was aimed to extend the postharvest conservation of 'Tommy Atkins' mango fruits harvested in break maturity stage. Fruits were submitted at the following treatments: hot water treatment (55°C for 5 minutes) and benomyl 1,000 mg.L-1; irradiation with 0,8 or 1,0 kGy; irradiation associated at carnaúba wax; and control. The fruits were stored at 10°C and 85 - 90%RH during 21 days, and then removed to ambient temperature (25,7±0,7°C and 87,1±2,2%RH). Through the storage time, the evolution of fresh weight, color, rottenness, total soluble solids (TSS), total titratable acidity (TTA), and TSS/TTA ratio were measured. 'Tommy Atkins' mango fruits can have shelf life notably increased, when they were submitted to hot water treatment (55°C for 5 minutes) or γ radiation (0,8 and 1,0 kGy), associated with carnaúba wax application, before cold storage. These treatments increased the fruit resistance at refrigerated storage, and improved shelflife after transferring to ambient temperature.

Efeito da sacarose e sorbitol na conservação in vitro de Passiflora giberti N. E. Brown

This work objectified the study of sucrose and sorbitol effect in the in vitro conservation for Passiflora giberti N. E. Brown, access. Therefore, an experiment was conducted in a completely randomized design to compare control treatment (standard MS) to MS medium supplemented with three sucrose concentrations (0, 15 and 30 g L -1) combined with three sorbitol concentrations (10, 20 and 40 g L -1), in a total of 10 treatments with 20 replicas. The experiment evaluation was carried out at 30, 60, 90 and 120 days of incubation, whereas the height of shoots (cm), number of roots, number and color of leaves were observed. The results showed the possibility to maintain passion-fruit microplants for a four months period under slow growth in MS medium supplemented with 10 or 20 g L -1 of sorbitol, without sucrose, and kept under 16 hours photoperiod (22 μ E m -2 s -1) and temperature of 27 ± 1°C. Sucrose sustained the longest development of the microplants. Root formation was affected by the sorbitol in the concentration of 40 g L -1 and by the absence of sucrose in the culture medium.

Ophthalmic patterns of captive brown brocket deer (Mazama gouazoubira)

Captive brown brocket deer (Mazama gouazoubira) were manually restrained to assess tear production by the Schirmer tear test I to measure intraocular pressure by applanation tonometry, to examine ocular conjunctival epithelial cells via cytologic and histologic samples, and to survey ocular conjunctival microflora by microbiologic culture. The mean value for the Schirmer tear test I was 8.9 ± 1.8 mm/min, and the mean intraocular pressure was 15.3 ± 3.1 mm Hg. Conjunctival epithelium contained stratified pavimentous layers of cells, and the microflora consisted of predominantly gram-positive bacteria. Copyright 2007 by American Association of Zoo Veterinarians.

Benzyladenine and Gibberellic acid pulse on postharvest of Calathea louisae cut foliage

Calathea louisae is an herbaceous ornamental plant native of Brazil whose cut foliage has potential to be used as a new product for the local ornamental market, as well as the international market, due to its decorative dark green leaves with greenish white splotches along midrib, and purple undersides. The objective of this study was to evaluate pulse treatments of benzyladenine and gibberellic acid for maintaining quality and extending keepability of foliage. The experiment was conducted in a complete randomized design with three replications (three stems in each vase) and seven treatments: distilled water (control) and pulsing cut petioles in benzyladenine or gibberellic acid for 4 h at 100, 250 and 500 mg L-1. The senescence symptoms were characterized by leaf rolling and a decrease in the angle formed between leaf and petiole as a response to water stress. Gibberellic acid or benzyladenine pulse treatments (250 and 500 mg L-1) significantly extended the longevity of cut foliage compared to the control. Gibberellic acid pulse (250 and 500 mg L-1) maintained leaves' green coloration and brightness for a longer time compared to control and benzyladenine pulse. Pulsing with gibberellic acid or benzyladenine (at all tested concentrations) maintained upright leaves for a longer time, showing a significantly higher angle between leaf and petiole compared to control. Foliage pulsed with gibberellic acid (500 mg L-1) showed a significantly higher leaf relative water content, a significantly smaller loss of accumulated fresh mass percentage compared to control and other gibbberellic acid and benzyladenine treatments.

Modelación para ajuste de la curva de lactación usando función lineal, no lineal y polinomios segmentados en ganados con alto grado de sangre Brown Swiss

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pulse treatments to extend the postharvest life of Ctenanthe setosa cut foliage

Marantaceae leaves have a contrasting color pattern and a decorative design which are interesting traits for its use as cut foliage. No postharvest research has been carried out on the potential as cut foliage for species of Ctenanthe, a member of the Marantaceae. Grey-maranta (Ctenathe setosa) is an herbaceous ornamental plant native of Brazil whose cut foliage has potential to be used as new crop product for local and international ornamental markets. The objective of this study was to evaluate pulse treatments to extend the longevity of foliage. The experiment was conducted in a complete randomized design with three replications and eight treatments: distilled water; pulsing cut petioles-ends in citric acid (pH = 2.8/1h); 2% sucrose (4h); 1% sucrose plus 200 mg L-1 citric acid (4h); 0.01% Tween® 20 (4h); 100 mg L -1 benzyladenine plus 0.01% Tween® 20 (4h); 100 mg L-1 gibberellic acid plus 0.01% Tween® 20 (4h); and maintenance of cut petiole in holding solution with commercial sodium hypochlorite (0.5 mL L-1). The senescence symptoms were mainly characterized by leaf rolling and by a decrease in the angle formed between leaf and petiole as a response to water deficit stress. Gibberellic acid or benzyladenine pulse treatments significantly extended longevity (6 days compared to control) and maintained leaves' green coloration and brightness for a longer time compared to control. However, only the benzyladenine plus Tween® 20 pulse maintained leaves in an erect form for a longer time, showing a significantly higher angle between leaf and petiole compared to control. Also, foliage pulsed with benzyladenine plus Tween® 20 showed a significant smallest loss of accumulated fresh mass percentage compared to control by the T test as to parallelism of treatments lines.

Postharvest characteristics and treatments to overcome latex exuded from cut flowers of lotus

Lotus (Nelumbo nucifera Gaertn.) is a perennial herbaceous aquatic ornamental plant with potential to be used as a new cut flower for the Brazilian ornamental market. It shows exotic and attractive flowers and has a strong market appeal, once it is known as a symbol of purity, holiness and immortality. However, flowers have a short-vase life. Lotus flower stem exudes a large quantity of sticky milky sap from the cut surface, which is produced in laticifers, spatially associated with both xylem and phloem. It has been reported that latex coagulates on the cut surface preventing or reducing water absorption and reducing flowers' vaselife, requiring treatments to stop the flow of latex. The objective of this study was to report observations of lotus postharvest characteristics and evaluate treatments to overcome latex flow. The experiment was conducted as a complete randomized design with three replications of four stems in each vase and eight treatments; a control (distilled water), pretreatment of cut stem-ends with hot water (40° C/1 minute), boiling water (3 seconds), isopropyl alcohol 90% (10 minutes) or citric acid (pH = 2.8/1h) and, maintenance of stems in a holding solution of Tween® 20 (0.01%), citric acid (200 mg L-1) or Tween® 20 (0.01%) plus citric acid (200 mg L-1). Treatments had no significant effect on flowers vaselife which was only about three days, although isopropyl alcohol, hot and boiling water completely stopped latex flow. Cut stem-ends pretreated with citric acid (pH = 2.8/1 h) showed a significantly higher relative water content of petals compared to others treatments. The senescence symptom of lotus cut flowers was mainly characterized by abscission of turgid petals and dehiscence of stamens without any visual change of petal color and brightness.

Unusually rapid growth of brown tumour in the mandible after parathyroidectomy associated with the presence of a supernumerary parathyroid gland

The aim of this study is to report the case of a quick growing brown tumour in the jaw after a parathyroidectomy due to the presence of a rare fifth parathyroid gland. The patient had chronic renal disease and the diagnosis was tertiary hyperparathyroidism. Thirty days after the parathyroidectomy, the patient returned with a significant increase in the tumour size. The suspicion of a supernumerary gland was confirmed by parathyroid scintigraphy. The treatment of brown tumour is dependent on the treatment of the hyperparathyroidism. However, curettage should be considered if a large lesion is disturbing mastication. In conclusion, this case should attract the attention of general practitioner dentists, since they may be the first professionals who have contact with the patient with a brown tumour in the jaws. Likewise, this case emphasises the importance of knowing the type of hyperparathyroidism involved to allow for effective treatment planning. © 2011 European Association for Cranio-Maxillo-Facial Surgery.