An initial ATP-independent step in the unwinding of a herpes simplex virus type I origin of replication by a complex of the viral origin-binding protein and single-strand DNA-binding protein

Using a spectrophotometric assay that measures the hyperchromicity that accompanies the unwinding of a DNA duplex, we have identified an ATP-independent step in the unwinding of a herpes simplex virus type 1 (HSV-1) origin of replication, Oris, by a complex of the HSV-1 origin binding protein (UL9 protein) and the HSV-1 single-strand DNA binding protein (ICP8). The sequence unwound is the 18-bp A + T-rich segment that links the two high-affinity UL9 protein binding sites, boxes I and II of Oris. P1 nuclease sensitivity of Oris and single-strand DNA-dependent ATPase measurements of the UL9 protein indicate that, at 37°C, the A + T-rich segment is sufficiently single stranded to permit the binding of ICP8. Binding of the UL9 protein to boxes I and II then results in the formation of the UL9 protein–ICP8 complex, that can, in the absence of ATP, promote unwinding of the A + T-rich segment. On addition of ATP, the helicase activity of the UL9 protein–ICP8 complex can unwind boxes I and II, permitting access of the replication machinery to the Oris sequences.

Valedictory poem delivered in the Chapel of Harvard University on the 21 June 1796: in the presence of the President Proffers & Tutors & the four classes of understudents at the request of the class who completed their college studies on that day

Handwritten draft of the Charles P. Sumner’s valedictory poem to the Harvard class of 1796, in a 19th century hardcover binding beginning “The youth by adverse fortune forced to roam…”. The poem mentions John Russell, a member of the Class of 1796 who died in November 1795. The copy includes edits and struck-out words.

" Lines upon the late proceedings of the College Government" collection, 1787-1917

This collection contains various manifestations of a humorous poem, most often called "Lines upon the late proceedings of the College Government," written by classmates John Quincy Adams and John Murray Forbes in 1787. Both Adams and Forbes were members of the class of 1787, and the poem recounts events surrounding the pranks and ensuing punishment of two members of the class behind them, Robert Wier and James Prescott. Wier and Prescott had been caught drinking wine and making "riotous noise," and they were publicly reprimanded by Harvard President Joseph Willard and several professors and tutors, including Eliphalet Pearson, Eleazar James, Jonathan Burr, Nathan Read, and Timothy Lindall Jennison. The poem mocks these authority figures, but it spares Samuel Williams, whom it suggests was the only professor to find their antics humorous.

Middle Miocene isotope stratigraphy and paleoceanographic evolution of the northwest and southwest Australian margins (Wombat Plateau and Great Australian Bight)

The benthic stable isotope record from ODP Site 761 (Wombat Plateau, NW Australia, 2179.3 m water depth) documents complete recovery of the middle Miocene delta13C excursion corresponding to the climatic optimum and subsequent expansion of the East Antarctic Ice Sheet. The six main delta13C maxima of the "Monterey Excursion" between 16.4 and 13.6 Ma and the characteristic stepped increase in delta18O between 14.5 and 13.9 Ma are clearly identified. The sedimentary record of the shallower ODP Sites 1126 and 1134 [Great Australian Bight (GAB), SWAustralia, 783.8 and 701 m water depth, respectively] is truncated by several unconformities. However, a composite benthic stable isotope curve for these sites provides a first middle Miocene bathyal record for southwest Australia. The delta18O and delta13C curves for Sites 1126 and 1134 indicate a cooler, better-ventilated water mass at ~700 m water depth in the Great Australian Bight since approximately 16 Ma. This cooler and younger water mass probably originated from a close southern source. Cooling of the bottom water at ~16 Ma started much earlier than at other sites of equivalent paleodepths in the central and western parts of the Indian Ocean. At Site 761, the delta18O curve shows an excellent match with the global sea level curve between ~11.5 and 15.1 Ma, and thus closely reflects changes in global ice volume. Prior to 15.1 Ma, the mismatch between the delta18O curve and the sea level curve indicates that delta18O fluctuations are mainly due to changes in bottom water temperature.

Phosphorus dynamics in waters of the Pacific Ocean

Tagged phosphorus was used to measure principal indices of mineral phosphorus variations in the euphotic zone of the East Pacific, i.e. total rate of uptake of phosphate phosphorus by microplankton (A_t), fraction consumed by phytoplankton (A_p/A_t), and turnover time (T). A_t reached its greatest values (150-280 ng/l/hour) in the upwelling zone of the Peru traverse, where development of phytoplankton was induced by upwelling. In other areas of this traverse values were 40-80 ng/l/hour in surface layers. In less productive waters on two other profiles (off Central America and California), values were lower, between 20 and 40 ng/l. On the vertical profile maxima of A_t were found at the upper boundary of the thermocline. Turnover time of PO4 phosphorus (T) in zones of phytoplankton abundance was very short, between 1.5 and 4 days. At most other stations it was 10-40 days, increasing to 100-200 days or longer at the lower boundary of the euphotic zone. In areas of phytoplankton abundance it accounted for 60-80% of total uptake of PO4 phosphorus. But in zones of elevated bacterial abundance, A_p/A_t fell to 20-40%. Data indicating lack of correlation between PO4 phosphorus and productivity are presented. It is emphasized that the above measures of PO4 phosphorus dynamics can be used for obtaining measures of functional condition and successional phase of marine plankton communities.

Kaye's and Malleson's History of the Indian mutiny of 1857-8 /

Vols. 3-6, by Malleson, first appeared under title: History of the Indian mutiny, 1857-1858, commencing from the close of the second volume of Sir John Kaye's History of the Sepoy War.

Shaker music : inspirational hymns and melodies illustrative of the resurrection life and testimony of the Shakers.

"Errata": p. [1] at end.

A catalog of the Napoleon library of De Paul university,

Mode of access: Internet.

Centons homériques : (Homerocentra) : Patricius, Eudocie, Optimus, Côme de Jérusalem /

Probably written over some time by various authors.

Défense des trois chapitres (à Justinien) /

Includes bibliographical references (p. [135]-138) and indexes.

The Napoleon myth. Containing a reprint of "The grand erratum,"

Mode of access: Internet.

A narrative of the campaign in Russia, during the year 1812.

Mode of access: Internet.

An authentic narrative of the invasion of France, in 1814.

Translation of the author's Histoire de la campagne de 1814, which was first published Paris, 1815 and republished Paris, 1816, as part 1 of his Histoire des campagnes de 1814 et de 1815.