微卫星DNA分析证明异种克隆大熊猫重构胚的核来自大熊猫供体

建立了一种从早期囊胚中提取DNA以进行核内和核外DNA分析的方法。用这种方法从异种克隆大熊猫重构胚中提取总DNA,以大熊猫特异的微卫星DNA引物成功地扩增出了微卫星座位g~(010)。PCR产物双向测序表明,2个重构胚与大熊猫供体对照序列完全一致。结果证明该异种大熊猫重构胚的核的确来自大熊猫供体细胞核。

猪小肠的综合利用与几种生化药物的制取

本文报道了腑衣、梅花肠、肝素钠、硫酸皮肤素、磷酸单酯酶、冠心舒和肠渣饲料蛋白的用途以厦利用猪小肠制取的方 法，并就综合利用问题进行了探讨。

乌鳢(Channa argus)干扰素刺激基因Viperin和ISG15及其启动子的克隆与特征分析

应用抑制差减杂交、末端快速扩增和基因步移技术,克隆并鉴定了乌鳢干扰素刺激基因Viperin和ISG15及其启动子序列.Viperin cDNA全长1474nt,包含1059nt的开放阅读框,编码352个氨基酸.除N末端70个氨基酸外,Viperin蛋白氨基酸序列在鱼类和哺乳类具有高度的保守性.ISG15 cDNA全长758nt,包含468nt的开放阅读框,编码155个氨基酸,含有两个泛素样(UBL)结构域,C末端具有保守的UB偶联结构(LRGG).Viperin和ISG15启动子区存在保守的干扰素刺激反应

五氯苯酚与腐殖酸作用的荧光猝灭效应研究

通过对五氯苯酚与腐殖酸作用的荧光猝灭效应研究,探讨了五氯苯酚与腐殖酸之间的相互作用机理．结果表明,五氯苯酚可以有规律地猝灭腐殖酸的内源荧光,其猝灭机理可认为是五氯苯酚和腐殖酸形成复合物的静态猝灭,五氯苯酚与腐殖酸的结合常数较大,说明腐殖酸能够有效吸附五氯苯酚分子,形成复合物;并获得了不同温度下,五氯苯酚与腐殖酸作用的结合常数和热力学参数．根据所得结果可推断五氯苯酚与腐殖酸的主要作用力为氢键和范德华力．

Multi-objective optimisation of horizontal axis wind turbine structure and energy production using aerofoil and blade properties as design variables

The design of wind turbine blades is a true multi-objective engineering task. The aerodynamic effectiveness of the turbine needs to be balanced with the system loads introduced by the rotor. Moreover the problem is not dependent on a single geometric property, but besides other parameters on a combination of aerofoil family and various blade functions. The aim of this paper is therefore to present a tool which can help designers to get a deeper insight into the complexity of the design space and to find a blade design which is likely to have a low cost of energy. For the research we use a Computational Blade Optimisation and Load Deflation Tool (CoBOLDT) to investigate the three extreme point designs obtained from a multi-objective optimisation of turbine thrust, annual energy production as well as mass for a horizontal axis wind turbine blade. The optimisation algorithm utilised is based on Multi-Objective Tabu Search which constitutes the core of CoBOLDT. The methodology is capable to parametrise the spanning aerofoils with two-dimensional Free Form Deformation and blade functions with two tangentially connected cubic splines. After geometry generation we use a panel code to create aerofoil polars and a stationary Blade Element Momentum code to evaluate turbine performance. Finally, the obtained loads are fed into a structural layout module to estimate the mass and stiffness of the current blade by means of a fully stressed design. For the presented test case we chose post optimisation analysis with parallel coordinates to reveal geometrical features of the extreme point designs and to select a compromise design from the Pareto set. The research revealed that a blade with a feasible laminate layout can be obtained, that can increase the energy capture and lower steady state systems loads. The reduced aerofoil camber and an increased L/. D-ratio could be identified as the main drivers. This statement could not be made with other tools of the research community before. © 2013 Elsevier Ltd.

Visible blind p(+)-pi-n(-)-n(+) ultraviolet photodetectors based on 4H-SiC homoepilayers

p(+)-pi-n(-)-n(+) ultraviolet photodetectors based on 4H-SiC homoepilayers have been presented. The growth of the 4H-SiC homoepilayers was carried out in a LPCVD system. The size of the active area of the photodetectors was 300 x 300 mu m(2). The dark and illuminated I-V characteristics had been measured at reverse biases form 0 to 20 V at room temperature, and the illuminated current was at least two orders of magnitude than that of dark current below 13 V bias. The peak value zones of the photoresponse were located at 280-310 nm at different reverse biases, and the peak value located at 300 nm was 100 times greater than the cut-off response value in 380 nm at a bias of 10V, which showed the device had good visible blind performance. A small red-shift about 5 nm on the peak responsivity occurred when reverse bias increased from 5 to 15 V. (c) 2006 Elsevier Ltd. All rights reserved.

Realization of low threshold of InGaAs/InAlAs quantum cascade laser

By optimizing the molecule beam epitaxy growth condition, the quality of quantum cascade (QC) material has greatly been improved. The spectrum of double x-ray diffraction indicates that the interface between the constituent layers is very smooth, the lattice mismatch between the epilayer and the substrate is less than 0.1%, and the periodicity fluctuation of the active region is not more than 4.2%. The QC laser with the emission wavelength of about 5.1 mum is operated at the threshold of 0.73 kA/cm(2) at liquid nitrogen temperature with the repetition rate of 10kHz and at a duty cycle of 1%. Meanwhile, the performance of the laser can be improved with suitable post process techniques such as the metallic ohmic contact technology.

零件库资源共享的公共语义模型

介绍了基于 Internet/ Intranet零件库资源共享公共语义模型 ,公共语义模型是对零件库信息建模和资源共享的业务方案的描述 ,是从需求向技术实现转变的媒介。进一步的工作是在公共语义模型的基础上 ,用形式化的方法和统一的数据格式表达零件库信息 ,建立零件库信息模型 ,以实现零件库资源共享。

原点无关最近特征分类器及在人脸识别中的应用

受最近特征线分类器的基本设计思想启发,将最近邻法向平面和空间推广,提出了原点无关最近特征平面和原点无关最近特征空间分类器.与最近特征平面、最近特征空间分类器相比,原点无关最近特征分类器最大的优势就在于其定义的特征子空间不依赖于原点位置,而仅由同模式的若干个特征点决定.这种定义提高了相应的模式相似性度量的合理性与有效性.以人脸识别为例,对多种分类器的性能进行比较.实验结果表明,原点无关最近特征分类器在识别率、稳定性等方面均优于同阶的最近特征分类器.

高速率并行光发射模块的研制

介绍了有关并行光发射模块设计与制作的最新进展，制作并测试了12信道并行光发射模块，单信道传输速率大于2．5Gbit／s（最高可达3Gbit／s），12信道并行总传输速率为30Gbit／s。模块采用波长为850nm的垂直腔面发射激光器（VCSEL）作光源，激光器与驱动电路芯片直接用Au丝连接，输出光束直接耦合进入12信道的光纤阵列中，在单信道8mA的工作电流下，可以测到最高为3Gbit／s的清晰眼图。