Mutagenicity of hydroxyurea in lymphocytes from patients with sickle cell disease

Hydroxyurea is commonly used in the treatment of myeloproliferative diseases and in patients with sickle cell disease (SCD). The use of this antineoplastic agent in patients with SCD is justified because of the drug's ability to increase fetal hemoglobin levels, thereby decreasing the severity of SCD. However, high doses or prolonged treatment with hydroxyurea can be cytotoxic or genotoxic for these patients, with an increased risk of developing acute leukemia. This danger can be avoided by monitoring the lymphocytes of patients treated with hydroxyurea. Cytogenetic tests are important endpoints for monitoring the physiological effects of physical and chemical agents, including drugs. In this work, we assessed the genotoxicity of hydroxyurea in short-term cultures of lymphocytes from SCD patients. Hydroxyurea was not cytotoxic or genotoxic at the concentrations tested in the G2 phase of the cell cycle. These results support the use of hydroxyurea in the treatment of SCD, although further work is necessary to understand the effects of this drug in vivo

Cytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitro

Mercury is a xenobiotic metal that is a highly deleterious environmental pollutant. The biotransformation of mercury chloride (HgCl2) into methylmercury chloride (CH3HgCl) in aquatic environments is well-known and humans are exposed by consumption of contaminated fish, shellfish and algae. The objective of the present study was to determine the changes induced in vitro by two mercury compounds (HgCl2 and CH3HgCl) in cultured human lymphocytes. Short-term human leukocyte cultures from 10 healthy donors (5 females and 5 males) were set-up by adding drops of whole blood in complete medium. Cultures were separately and simultaneously treated with low doses (0.1 to 1000 µg/l) of HgCl2 and CH3HgCl and incubated at 37ºC for 48 h. Genotoxicity was assessed by chromosome aberrations and polyploid cells. Mitotic index was used as a measure of cytotoxicity. A significant increase (P < 0.05) in the relative frequency of chromosome aberrations was observed for all concentrations of CH3HgCl when compared to control, whether alone or in an evident sinergistic combination with HgCl2. The frequency of polyploid cells was also significantly increased (P < 0.05) when compared to control after exposure to all concentrations of CH3HgCl alone or in combination with HgCl2. CH3HgCl significantly decreased (P < 0.05) the mitotic index at 100 and 1000 µg/l alone, and at 1, 10, 100, and 1000 µg/l when combined with HgCl2, showing a synergistic cytotoxic effect. Our data showed that low concentrations of CH3HgCl might be cytotoxic/genotoxic. Such effects may indicate early cellular changes with possible biological consequences and should be considered in the preliminary evaluation of the risks of populations exposed in vivo to low doses of mercury.

Avaliação in vitro dos possíveis efeitos citotóxicos, genotóxicos e mutagênicos das drogas antimaláricas artemisinina e artemeter em linfócitos humanos

A artemisinina é uma substância extraída da planta chinesa Artemisia annua L., sendo bastante utilizada na medicina natural como um terapêutico em várias patologias. Já o artemer é uma substância sintetizada a partir da artemisinina. Estas drogas se enquadram em um grupo especial de moléculas denominadas de lactonas sesquiterpênicas sendo amplamente administradas na terapêutica da malária. Embora sejam considerados eficientes anti-maláricos, muito pouco se sabe sobre os efeitos genotóxicos e citotóxicos destes fármacos. Portanto, no presente trabalho, avaliamos os efeitos citotóxicos, genotóxicos e mutagênicos da artemisinina e do artemeter em cultura de linfócitos humanos por meio do ensaio cometa, do teste do micronúcleo e do ensaio de citotoxicidade para detecção de necrose e apoptose por marcação fluorescente diferencial com laranja de acridina/brometo de etídio (LA/BE), respectivamente. Nossos resultados demonstraram um aumento significativo (p<0,05) no índice de dano do DNA avaliado pelo ensaio do cometa, bem como na frequência de micronúcleos em ambas as substâncias testadas. Foi observado também, que apenas a artemisinina induziu um aumento estatisticamente significativo (p<0.05) no número de células necróticas nos linfócitos em 48 h de tratamento. Desta forma, demonstrou-se em nosso trabalho, que estas duas drogas exercem efeitos citotóxicos, genotóxicos e mutagênicos em culturas de linfócitos humanos, nas condições avaliadas. Nossos dados apontam a necessidade de cautela no uso de tais medicamentos, uma vez que efeitos genotóxicos/mutagênicos podem aumentar o risco de carcinogênese.

O integralismo pliniano: autoritarismo e ordem na defesa da nação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A universalidade poética de Rilke na formação do Grupo dos Novos: tradução e confluências na Amazônia brasileira

Esta dissertação objetiva pensar os desdobramentos que as traduções da poesia de Rainer Maria Rilke alcançaram no cenário do Suplemento Literário da Folha do Norte - SL/FN e em outros periódicos das décadas de 1940 e 1950, em que foram publicadas. Consideramos que estas traduções foram um indício de intensificação da abertura universalizante das letras paraenses às novidades modernistas iniciadas na Semana de Arte Moderna de 1922. Tais traduções foram cruciais para a formação do Grupo dos Novos, composto principalmente de nomes como Benedito Nunes, Mário Faustino e Paulo Plinio Abreu, além do antropólogo alemão Peter Paul Hilbert. O suporte teórico-metodológico será a utilização dos conceitos de Weltliteratur (Literatura universal) de Goethe e Reflexionsmedium (Médium-de-reflexão) de Benjamin. Estas matrizes interligarão os campos da história, da filosofia da linguagem e da tradução para refletirmos sobre o impacto dessas traduções na Amazônia brasileira da época. Outro conceito importante nesta dissertação é o de Bildung (formação), pensado a partir da leitura de Antoine Berman quando ele o relaciona à tradução, incluindo vários fenômenos que envolvem as relações tradutórias entre culturas e línguas. Ao final, por meio da reflexão tradutológica de Haroldo de Campos, visamos observar possíveis confluências nas produções poéticas e críticas de Mário Faustino, Manuel Bandeira e Paulo Plínio Abreu, como resultante do processo relacional promovido por estas traduções.

Análise dos processos e instrumentos de ordenação do território: comparação entre Brasil e Portugal

Pós-graduação em Geografia - IGCE

O romance policial do século XXI: manutenção, transgressão e inovação do gênero

Ao estudar os romances policiais mais vendidos no Brasil entre 2000 e 2007, Fernanda Massi constatou um grande distanciamento entre esses textos e os romances policiais tradicionais. Nos livros escritos por autores clássicos como Agatha Christie, Conan Doyle e Georges Simenon, há algumas características recorrentes: um crime de autoria desconhecida, um criminoso com motivos para assassinar e um detetive encarregado de encontrar a identidade do criminoso e entregá-lo à polícia para que receba a merecida punição. Nesta obra, Massi nota que autores contemporâneos como Rubem Fonseca, Luiz Alfredo Garcia-Roza e Dan Brown, entre outros, mantêm a tríade criminoso-vítima-detetive, pois ela é indispensável ao enredo. Mas utilizam o núcleo do romance policial para abordar outros temas, como adultério, corrupção em órgãos públicos, segredos de fundo religioso ou deterioração familiar. Assim, segundo a autora, esses escritores escrevem com uma liberdade característica da pós-modernidade: desenvolvem a narrativa seguindo o modelo fixo de estrutura do gênero policial, porém de modo vivo e adaptado à contemporaneidade, inclusive no que concerne aos valores morais. O diagnóstico alcançado lança nova luz sobre o cenário contemporâneo da literatura policial.

De Holmes a Poirot: relações entre literatura e história na narrativa policial britânica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of surface treatments on the shear bond strength of luting cements to Y-TZP ceramic

Statement of problem Because zirconia is a glass-free material, alternative surface treatments such as airborne-particle abrasion or silica coating should be used for long-term bonding. However, these surface treatments in combination with different bonding agents and luting cements have not yet been studied. Purpose The purpose of the study was to evaluate the effect of surface treatments on the shear bond strength (SBS) of luting cements to Y-TZP ceramic. Material and methods Zirconia disks (N=240) were airborne-particle abraded with the following particles (n=48): 50 μm Al2O3; 120 μm Al2O3; 30 μm silica-coated Al2O3 (Rocatec Soft); 120 μm Al2O3+110 μm silica-coated Al2O3 (Rocatec Plus); and Rocatec Plus. After silanization of the zirconia surface, composite resin disks were bonded with (n=12) RelyX Luting 2; RelyX ARC; RelyX U100; and Panavia F. The bonded specimens were thermocycled (10 000 cycles) and tested for SBS. Failure mode was determined with a stereomicroscope (×20). The morphology and elemental composition of airborne-particle abraded surfaces were evaluated with scanning electron microscopy (×500) and energy-dispersive x-ray spectroscopy (×50). Results Surface treatments, cements, and their interaction were significant (P<.001). For RelyX ARC, Rocatec Soft and Rocatec Plus provided the highest SBS. In general, surface treatments did not influence the SBS of RelyX U100 and Panavia F. Regardless of the cement, no significant difference was found between 50 μm and 120 μm Al2O3 particles, between Rocatec Soft and Rocatec Plus, or between Rocatec Plus and 120 μm Al2O3 particles+Rocatec Plus. All groups showed adhesive failures. Different particle sizes provided differences in morphological patterns. The elemental composition comprised Al and Al/Si for alumina and silica-abraded zirconia. Conclusions Particle size did not influence the SBS of the groups abraded exclusively with alumina or silica-coated particles. RelyX ARC was more surface-treatment dependent than RelyX U100 or Panavia F.

A dialética da malandragem revisitada em O Xangô de Baker Street

Using the concept of influence, well explored by Sandra Nitrini (2000), the purpose of this study is to verify the characterization of the main characters of O Xangô de Baker Street (1995) and Macunaíma, o herói sem nenhum caráter (1928) – Sherlock Holmes and Macunaíma – as rogues in their novels, heirs to the Spanish picaresque, a genre that has taken on new meaning in Brazilian literature, as pointed out since the 1960s by Antonio Candido in his famous essay “Dialética da malandragem” (2010). With these theories, we analyze the composition of the tricksters, whose starting point is the question facing the country, the Brazilian. Although the distances in time and style of separating the protagonists created by Jô Soares and Mário de Andrade, we have noticed the interesting procedure of re-creation of the renowned English detective created by Arthur Conan Doyle and the reinterpretation of aspects of Brazilian folklore a convergence of interests with regard to representation of the national character.

Combined measurement of the Higgs Boson Mass in pp collisions at root s=7 and 8 TeV with the ATLAS and CMS experiments

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Um conceito plural: a ἄτη na tragédia grega

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ocorrência de Clostridium botulinum tipos C e D em criatórios de bovinos no município de Cocalinho, Vale do Araguaia, Mato Grosso

In order to assess the occurrence and distribution of spores and toxins of Clostridium botulinum types C and D in three farms in Cocalinho, at the Araguaia River valley, State of Mato Grosso, Brazil, we analyzed sediment samples from 40 water holes, soil and cattle feces, collected around water holes. Sediments were analyzed by direct method, whilst feces, soil and also sediment samples were individually analyzed by indirect method. The detection of spores and botulinum toxins in the filtered material was performed by bioassay in Swiss Webster mice strain, as well as the serum-neutralization of the positive materials for typing. Samples of cattle feces showed the largest positive rate for C. botulinum, with 25/40 (62.5%), followed by soil, 12/40 (30%), and by sediment, 13/40 (32.5%). From the 40 cattle feces samples, 25 (62.00%) were positive for Clostridium botulinum; six samples were identified as type C, other six as type D, and 13 samples were classified as CD complex. From the equal number (40) of soil samples, 12 (30%) were positive for C. botulinum; two samples were identified as type C, other three as type D, and seven samples were classified as CD complex. Regarding the 40 sediment samples, 13 (32.5%) were positive for C. botulinum; two samples were identified as type C, other three as type D, and eight samples were classified as CD complex. No botulism toxin was detected by indirect method.

Importância da avaliação do ciclo de vida na análise de produtos: possíveis aplicações na construção civil

Much has been discussed lately about reusing waste in new construction materials and countless studies have been carried out based on this objective. However, before these products can be called “sustainable” and a recycling system can be effectively implemented, it is fundamental for all the aspects involved in this action to be understood, and more important, what are the true gains for the productive sector and what are the benefits for the environment if this was to occur. In order to obtain a general framework for all environmental aspects and potential impacts associated with these products, over their entire life cycle, the most appropriate methodology is the Life Cycle Assessment (LCA). In view of the above, the objective of this work is to provide an overview of current studies of LCA, its history, its importance, its standards and the methodology employed and its applications related to civil construction. It is expected, to obtain a more complete scenario of the influences from a study of LCA, as well as establishing a contribution for the assessment of specific products and processes for civil construction, disseminating the importance of the use of this tool within the sector.