Introdução de Lentinulaedodes (Shiitake) e Pleurotusostreatus (Shimeji) em bagaço de cana termo explodido para diminuição da toxicidade

Ethanol production has gained great prominence in the investment new renewable energy sources and Brazil is among the leaders of production. However, this activity generates large amounts of waste being the largest volume of the sugar cane bagasse. For this reason looking up ways to use this material as burning for energy production and composition of forage in the diet of ruminants, however there are difficulties to use this production for this last one. This paper proposes a microbiological treatment with Lentinula edodes and Pleurotus ostreatus in order to enable the bagasse in ruminant feed composition in order to be used more noble than their burning. After treatment with the fungus, tests were performed for quantifying crude protein by the method of Kjeldhal. It was verified that the protein content in the pure bagasse was 1.0% after fermentation the protein content was 4.2% with L.edodes and 4.9% with P. ostreatus. To evaluate the protein quality of the product fermented by L. edodes and P. ostreatus was applied microbiological method for growth of Enterococcus zimogenes verifying that after fermentation the protein quality was 76 and 27.4% with L. edodes and P.ostreatus, respectively, compared with casein. The quantification of amino acids showed significant improvement of protein with altered amino acid profile with treatments of fungos. About of DQO and BOD were also found considerable improvement besides considerable drop in toxicity as measured by acute toxicity test with Daphinia similis

Neoplasias mamárias de cadelas: expressão gênica e proteica da via Wnt/b-catenina, sua associação com a transição epitélio-mesênquima e prognóstico

Pós-graduação em Medicina Veterinária - FCAV

Ação do hormônio triodotironina (T3) altera a expressão gênica do oncogene Amphiregulin (Areg) el células tumorais de adenocarcinoma de mama

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Condição corporal e jejum sobre o metabolismo energético e crescimento de juvenis de pacu

Pós-graduação em Zootecnia - FCAV

Indicadores antropométricos do estado nutricional de pré-escolares em Araraquara, SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização clínica, demográfica e nutricional de pacientes oncológicos atendidos em um hospital público - 2008

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Obtenção de anticorpos monoclonais murinos contra antígenos de Staphylococcus aureus resistentes à meticilina por imunização subtrativa

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Abordagem fotomorfogenética para explorar o estresse abiótico em tomateiro (Solanum lycopersicum L.)

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Efeitos da adição de agentes tróficos na dieta de leitões desmamados sobre a expressão da enzima ornitina descarboxilase, os conteúdos de proteína e DNA e o desempenho

Two experiments were carried out to evaluate the effect of adding glutamine, fish oil or yeast cellular wall to the diet of weaned piglets on the expression of the enzyme ornithine decarboxylase (ODC), the protein content and DNA in small intestine samples and on performance. In the first experiment, 24 weaned piglets were used to measure the performance at the phases prestarter, starter 1 and starter 2. Four diets were tested (T1 -basal diet (BD); T2 -BD + 1% of glutamine; T3 -BD + 0,2% of yeast cellular wall; T4 -BD + 5% of fish oil). At the second experiment 45 weaned piglets were used and distributed in a randomized block design, in factorial outline with four diets and three slaughter ages (on the day of weaning, on the seventh and fourteenth days postweaning). The tested diets did not alter the piglets' performance in none of the phases. There was reduction of the expression of the ODC enzyme, of the protein concentration and of the relationship protein/DNA to the seven days postweaning, with increase of the values on the 14th day, evidencing a state of hypotrophy of the mucous membrane, suggesting that the process of protein synthesis in the small intestine was diminished in the first week after weaning, but it presented recovery signs in the second week.

Análise celular e molecular da ação do peptídeo Ac2-26 da proteína anti-inflamatória Anexina A nas células de carcinoma de colo de útero

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do tratamento da H. pylori na expressão de citocinas e microRNAs associados ao processo inflamatório

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência de diferentes dietas na densidade óssea de calopsitas (Nymphicus hollandicus)

Pós-graduação em Ciência Animal - FMVA

Exercício físico resistido em pacientes com doença renal crônica em hemodiálise: ensaio clínico randomizado controlado

Pós-graduação em Fisioterapia - FCT

Efeitos preventivos do exercício físico sobre as proteínas ciclooxigenase-2 (COX-2) e fator de crescimento de vasos derivado do endotélio (VEGF) no músculo cardíaco em ratos tratados com dexametasona

Dexamethasone (DEXA) is a synthetic glucocorticoid widely used in the handling of several drugs, for its proven benefits in fighting inflammation and allergies. Despite their benefits, their chronic use leads to several side effects that include changes in the body in the metabolism of carbohydrates, lipids and proteins. Moreover, being an anti-inflammatory, acts on the arachidonic acid pathway, reducing the expression of the enzyme cyclooxygenase (COX-2) and growth factor derived from the endothelium of blood vessels (VEGF) in various tissues. However, its effects on the myocardium are still uncertain. The physical training (PT), in turn, promotes effects contrary to those caused by chronic use of DEXA, however, little is known about the preventive effects of TF in the side effects of Dexa in the myocardium. Therefore, the aim of this study was to determine if the TF has the ability to prevent and/or mitigate the effects of Dexa in protein expression of COX-2 and VEGF in the myocardium. Forty animals were divided into 4 groups: sedentary control (SC), sedentary treated with Dexa (SD), trained control (TC) and Trained treated with Dexa (TD) and submitted to a protocol of physical training on the treadmill for 70 days (1 h/day-5 days per week, 60% of physical capacity) or kept sedentary. Over the past 10 days, rats were treated with Dexa (Decadron, 0.5 mg/kg per day, ip) or saline. During training the animals were weighed weekly and during treatment daily. At the end of treatment was made to measure fasting glucose levels of animals. The rats were killed with excess anesthesia and cardiac muscle was removed, weighed, homogenized, centrifuged and stored at -20° C for analysis of protein expression of VEGF and COX-2 by Western blotting technique. Treatment with dexamethasone caused a weight loss of 18% in sedentary animals and 13% in trained as well as elevated levels of fasting glucose in sedentary (88%). The TF was unable to mitigate the loss in...

Efeitos preventivos do exercício físico na expressão da miostatina em ratos tratados com Dexametasona

Muscle atrophy is always associated with Dexamethasone (Dexa) treatment, however the mechanisms are not completely understood. This study investigated the effects of Dexa on myostatin and p70S6K protein expression and if previous exercise training (T) can attenuate these effects. Eighty rats were distributed into 4 groups: sedentary control (SC), sedentary treated with Dexa (SD; 0,5 mg/kg per day, i.p., 10 days), trained control (TC) and trained treated with Dexa (TD) and underwent a training period where they were either submitted to a running protocol (60% of physical capacity, 5 days/week for 8 weeks) or kept sedentary. After T period, animals underwent Dexa treatment concomitant with training. Western Blot was performed to identify myostatin and p70S6k protein expression in the tibialis anterior (TA) and soleus (SOL) muscle. Ten days of Dexa treatment increased fasting glucose (SD=+62%), however previous T attenuated this increase (TD=+20%, p<0.05). Dexa determined significant decrease in body weight in TD (-22%) and SD (-25%), followed by TA weight reduction in SD (-23%) and TD (-20%). Previous training could not avoid these decreases. Myostatin protein expression was not altered by dexa treatment or training in TA muscle but in SOL muscle it was significantly modified after T, regardless of treatment (TC=+%23 and TD=+25) compared with their respective controls. The protein p70S6K was not modified neither by dexa nor training in any of the analyzed muscle or condition. The results of this study allowed us to conclude that previous training attenuates the hyperglycemia induced by Dexa, however it did not prevent the body or muscle weight reductions. Even in the presence of muscle atrophy, the expression of myostatin and p70S6K do not justify the mechanisms of muscle loss induced by Dexa, which suggests that other catabolic or anabolic proteins could be involved in the process of muscle atrophy after 10 days of treatment with Dexa