Regulation of apoptosis during the pathogenesis of non -melanoma skin cancer

Previous studies from our lab have shown distinctive patterns of expression of bcl-2 gene family members in human nonmelanoma skin cancer (NMSC). To further evaluate the significance of these observations and to study the effects of cell death deregulation during skin carcinogenesis, we generated a transgenic mouse model (HK1.bcl-2) using the human keratin 1 promoter to target the expression of a human bcl-2 minigene to the epidermis. Transgenic protein expression was confirmed in all the layers of the epidermis except the stratum corneum using immunohistochemistry. Multifocal epidermal hyperplasia, without associated hyperkeratosis, was observed in newborn HK1.bcl-2 mice. Immunofluorescence staining using monoclonal antibodies specific for a variety of differentiation markers revealed aberrant expression of keratin 6 (K6) in the transgenic epidermis. Epidermal proliferative indexes, assessed by anti-BrdUrd immunofluorescence staining, were similar in control and transgenic newborn mice, but suprabasal proliferating cells were seen within the hyperplastic areas of the transgenic mouse skin. Spontaneous apoptotic indices of the epidermis were similar in both control and HK1.bcl-2 transgenic newborn mice, however, after UV-B irradiation, the number of "sunburn cells" was significantly higher in the control compared to the HK1.bcl-2 transgenic animals.^ Adult HK1.bcl-2 and control littermate mice were used in UV-B and chemical carcinogenesis protocols including DMBA + TPA. UV-B irradiated control and HK1.bcl-2 mice had comparable incidence of tumors than the controls, but the mean latency period was significantly shorter in the HK1.bcl-2 transgenic. Both control and transgenic animals included in chemical carcinogenesis protocols required application of both the initiating (DMBA) and promoting (TPA) agents to develop tumors. The frequency, number, and latency of tumor formation was similar in both groups of animals, however, HK1.bcl-2 mice exhibited a rate of conversion from benign papilloma to carcinoma 2.5 times greater than controls.^ Similar carcinogenesis experiments were performed using newborn mice. HK1.bcl-2 mice treated with UV-B plus TPA have a three fold greater incidence of tumor formation compared to controls littermates. HK1.bcl-2 transgenic animals also exhibited a shorter latency for papilloma formation when treated with DMBA plus TPA.^ HK1.bcl-2/v-Ha-ras double transgenic mice shared phenotypic features of both HK1.v-Ha-ras and HK1.bcl-2 transgenic mice, and exhibited focal areas of augmented hyperplasia. These double transgenic mice were susceptible to tumor formation by treatment with TPA alone.^ Cultures of primary keratinocytes were established from control, HK1.bcl-2, HK1.Ha-ras, and HK1.bcl-2/v-Ha-ras newborn mice. Cell viability was determined after exposure of the cells to UV-B irradiation, DMBA, TPA, or TGF-$\beta$1. Internucleosomal DNA fragmentation ("ladders") and morphological cellular changes compatible with apoptotic cell death were observed after the application of all these agents. HK1.bcl-2 keratinocytes were resistant to cell death induction by all of these agents except TGF-$\beta$1. HK1.Ha-ras cells had a higher spontaneous rate of cell death which could be compensated by co-expression of bcl-2.^ These findings suggest that bcl-2 dependent cell death suppression may be an important component of multistep skin carcinogenesis. ^

Apoptosis and the molecular complementation of bcl-2, myc and p53 in multistep lymphomagenesis

Follicular lymphoma is the most common lymphoid malignancy in humans. The bcl-2 transgenic mice, which mimic the human follicular lymphoma, initially exhibit a polyclonal hyperplasia due to the overriding of apoptosis by deregulated bcl-2. After a latency period of 15 month 20% of the animals developed clonal lymphomas. Approximately, 50% of these high grade lymphomas presented chromosomal translocations involving c-myc, suggesting that deregulation of this gene is important in the complementation with bcl-2. E$\mu$-myc x bcl-2 double transgenic mice were generated to assess the ability of this two genes to complement in an in vivo system. The double transgenic mice presented a shortened latency (3-4 weeks) and higher incidence of tumor development. Quantification of the extent of programmed cell death indicated that bcl-2 can abrogate the high rate of apoptotic cell death that results from myc deregulation. Bcl-2-Ig, E$\mu$-myc, and bcl-2/E$\mu$-myc lymphomas were examined using PCR-SSCP to detect the presence of p53 mutations in exons 5-9. A high incidence of p53 mutations in E$\mu$-myc lymphomas suggested that inactivating lesions of p53 may represent an important step in the genetic complementation of c-myc in lymphomagenesis. Surprisingly, p53 mutations were quite uncommon in bcl-2 lymphomas suggesting that inactivating mutations of p53 and overexpression of bcl-2 may not cooperate in lymphoma progression. To assess this question, we generated mice that contained a deregulated bcl-2 gene and were nullizygous for p53 (p53KO). No reduction in the tumor latency was observed in the p53KO/bcl-2-Ig hybrid mice when compared with p53 KO mice. Using splenic mononuclear cells isolated from p53KO mice and bcl-2 transgenic mice we demonstrated that bcl-2 suppresses p53 mediated apoptosis in response to DNA damage initiated by $\gamma$-radiation even though p53 protein is induced normally in the bcl-2 overexpressing cells. Western analysis of the expression of p53 target proteins after $\gamma$-radiation showed a correlation between the p53-dependent induction of bax protein after radiation and the ability of p53 to mediate apoptosis. ^

Fas ligand and soluble Fas -opposing molecules in melanoma lung metastasis

Deregulation of apoptotic cell death can result in aberrant accumulation of cells and increased tumor incidence. Fas (CD95) and Fas ligand (FasL) are a receptor-ligand pair whose activation induces apoptosis in many cell types. Previously, we demonstrated that low metastatic, Fas+ K1735-P murine melanoma cells spontaneously metastasize to the lung following orthotopic injection into FasL-deficient (gld) mice compared to wild-type (wt) controls. We further demonstrated that the expression of the Fas antagonist soluble Fas (sFas) directly correlates with disease stage in patients with melanoma, breast, and colon cancer. These findings document a role for host-derived FasL, in the control of metastatic disease and suggest a role for tumor-associated sFas in acquiring metastatic potential. To directly test whether FasL expressed on lymphocytes or on lung stromal cells restricts metastasis, bone marrow chimeras were generated between C3H wt and C3H gld mice. Chimeric animals were injected subcutaneously with 5 × 105 K1735-P and the incidence and number of spontaneous lung metastases scored. The data show that wt mice receiving gld marrow had a greater number of lung metastases (median 9.5, range 2–31) than gld mice reconstituted with wt marrow (median 1, range 0–31; p < 0.016). Interestingly, both groups had fewer metastases compared to gld controls (median 18.5, range 0–46) but more than wt controls (median 2, range 0–7). These observations provide the first evidence that both hematopoietic- and nonhematopoietic-host derived FasL, are important in the control of melanoma metastasis to the lung. To directly test whether tumor-associated sFas expression can enhance metastasis, K1735-P cells were transfected with three isoforms of sFas (Exo4Del, Exo6Del, and Exo3, 4, 6Del). RT-PCR and ELISA analysis confirmed the expression of sFas RNA and protein respectively. Following intravenous injection of 5 × 104 cells, sFas transfected cells formed significantly more experimental lung metastases [Exo6Del clone 3 (median 22, range 0–36), Exo6Del clone 7 (median 31, range 4–50), Exo3, 4, 6Del (median 22.5, range 13–48)] compared to vector control cells (median 6.5, range 3–29). Together, these data provide the first evidence that sFas is sufficient to enhance the metastatic potential of Fas+ melanoma cells. ^

Molecular mechanisms involved in the regulation of cell polarity and genome stability by the p21 -activated kinase, Shk1, in the fission yeast, Schizosaccharomyces pombe

The essential p21-activated kinase (PAK), Shk1, is a critical component of a Ras/Cdc42/PAK complex required for cell viability, normal cell polarity, proper regulation of cytoskeletal dynamics, and sexual differentiation in the fission yeast, Schizosaccharomyces pombe. While cellular functions of PAKs have been described in eukaryotes from yeasts to mammals, the molecular mechanisms of PAK regulation and function are poorly understood. This study has characterized a novel Shk1 inhibitor, Skb15, and, in addition, identified the cell polarity regulator, Tea1, as a potential biological substrate of Shk1 in S. pombe. Skb15 is a highly conserved WD repeat protein that was discovered from a two-hybrid screen for proteins that interact with the catalytic domain of Shk1. Molecular data indicate that Skb15 negatively regulates Shk1 kinase activity in S. pombe cells. A null mutation in the skb15 gene is lethal and results in deregulation of actin polymerization and localization, microtubule biogenesis, and the cytokinetic machinery, as well as a substantial uncoupling of these processes from the cell cycle. Loss of Skb15 function is suppressed by partial loss of Shk1, demonstrating that negative regulation of Shk1 by Skb15 is required for proper execution of cytoskeletal remodeling and cytokinetic functions. A mouse homolog of Skb15 can substitute for its counterpart in fission yeast, demonstrating that Skb15 protein function has been substantially conserved through evolution. ^ Our laboratory has recently demonstrated that Shk1, in addition to regulating actin cytoskeletal organization, is required for proper regulation of microtubule dynamics in S. pombe cells. The Shk1 protein localizes to interphase and mitotic microtubules, the septum-forming region, and cell ends. This pattern of localization overlaps with that of the cell polarity regulator, Tea1, in S. pombe cells. The tea1 gene was identified by Paul Nurse's laboratory from a screen for genes involved in the control of cell morphogenesis in S. pombe. In contrast to wild type S. pombe cells, which are rod shaped, tea1 null cells are often bent and/or branched in shape. The Tea1 protein localizes to the cell ends, like Shk1, and the growing tips of interphase microtubules. Thus, experiments were performed to investigate whether Tea1 interacts with Shk1. The tea1 null mutation strongly suppresses the loss of function of Skb15, an essential inhibitor of Shk1 function. All defects associated with the skb15 mutation, including defects in F-actin organization, septation, spindle elongation, and chromosome segregation, are suppressed by tea1Δ, suggesting that Tea1 may function in these diverse processes. Consistent with a role for Tea1 in cytokinesis, tea1Δ cells have a modest cell separation defect that is greatly exacerbated by a shk1 mutation and, like Shk1, Tea1 localizes to the septation site. Molecular analyses showed that Tea1 phosphorylation is significantly dependent on Shk1 function in vivo and that bacterially expressed Tea1 protein is directly phosphorylated by recombinant Shk1 kinase in vitro. Taken together, these results identify Tea1 as a potential biological substrate of Shk1 in S. pombe. ^ In summary, this study provides new insights into a conserved regulatory mechanism for PAKs, and also begins to uncover the molecular mechanisms by which the Ras/Cdc42/PAK complex regulates the microtubule and actin cytoskeletons and cell growth polarization in fission yeast. ^

La actividad hidrocarburífera en la Cuenca del Golfo San Jorge : El papel del empresariado regional

La política económica que se implementó en la década de 1990 tuvo como eje, entre las principales medidas, la apertura de la economía, la privatización de las empresas públicas, el desmantelamiento del aparato estatal y sus agentes reguladores, el ajuste fiscal y del gasto público tanto a nivel nacional, como provincial y municipal, la retirada del Estado Nacional como inversor y subsidiador de actividades económicas, la desregulación del mercado de trabajo y la concentración en grupos 'oligopólicos' de las principales actividades productivas, lo que produjo diferentes impactos en las distintas actividades económicas de la Región Patagónica, dependiendo de su inserción en el mercado interno y externo. En este trabajo se pretende, a partir de una descripción de la evolución de la actividad petrolera y de un estudio de caso, analizar los factores que viabilizan el desarrollo económico local y repensar el papel de los actores sociales en relación con el contexto y con las posibilidades de establecimiento de estrategias de innovación en las sociedades locales. En la primera parte del artículo se hace una caracterización socioeconómica de la región de la Cuenca del Golfo San Jorge. A continuación se observa la evolución de la actividad petrolera dentro de la cuenca, su papel en el mercado de trabajo y su requerimiento de mano de obra; se realiza una caracterización de los principales actores y de su relación con el desarrollo local. En la parte final del artículo se intenta mostrar los desafíos que enfrentan los actores públicos y privados vinculados con la actividad petrolera y su proyección en el corto y mediano plazo

De siderúrgica a turística : Breve historia ocupacional de la ciudad de Palpalá (Jujuy-Argentina)

The growth of Palpalá City (Province of Jujuy, argentina) since the 1940s, is linked to altos Hornos Zapla (ahz) the state-owned steel and iron company whose privatization in the early 1990s as part of the external three-sided opening, privatizations and deregulation which characterized convertibility, meant large changes in the local economic structure. In the 1950s, this city was identified by the town hall as 'mother of Industries' ('madre de Industrias'), whereas today, the municipal slogan is 'City of Tourism' ('Ciudad Turística'). Recovering the recent occupational history of this city meets the need to know the labor and socioeconomic reality of this urban area in the Province of Jujuy, about which information is rare. Although it is included in the Home Survey?s sample, it is shown in blocks, making the information very limited. It is paradigmaticin this city to study the means chosen to try to overcome the traumatic shock that ahz's privatization meant, to analyze the policies developed and evaluate their results, some years after their application. In Palpalá, a local development strategy was applied, with a strong impulse to micro-businesses, following, somehow, the model postulated at national level, what will allow us to know the local reality better, as well as the regional and national realities for its characteristics. The methodology used in this work ('From iron and steel industry to tourist city. Brief occupational history of Palpala city) was the bibliographic research of the rare existing studies, interviews to qualified informers as well as the use of secondary data sources, such as data from different national Censuses. To conclude, it can be said that due to the crisis of the 1990s and its state reducing plan, those who suffered its consequences less are, paradoxically, those who are still related to state jobs. In Palpalá, when ahz was privatized and the buyer company failed to fulfill its contract duly, leaving a great number of the local people unemployed, it was the municipal government who had to assume the responsibility of an answer ( in this case, through training, credit and/or counseling to micro-businessmen) In the last years, however, the possibility to work in informal activities has become important in Palpalá, with a high percentage of people working in city fairs selling different kinds of goods. although the change in the model in the recent years has allowed the reactivation in different areas, a preliminary evaluation is that the improvement does not seem to have reached the core of excluded and marginalized of the previous decade

Desarrollo, territorio y desigualdad en la globalización : Conflictos actuales en la agricultura familiar del nordeste de Misiones, Argentina

En el presente trabajo buscamos poner en cuestión la relación entre desarrollo, desigualdad y territorio en el contexto actual de la globalización y en el marco de las políticas públicas dirigidas, supuestamente, a favorecer a los actores sociales de menores recursos. Para ello nos centramos en un estudio de caso en el nordeste de la provincia de Misiones, Argentina. Aquí la actividad tabacalera, la forestación y la agricultura familiar (AF) alternativa (orientada a la producción de alimentos) entran en disputa por el territorio, dando lugar a conflictos por la ocupación de tierras privadas. Su análisis y devenir evidencia: (a) Las transformaciones territoriales resultantes de los procesos de avance de la globalización en ámbitos locales. (b) Los modos en que dichas transformaciones condicionan y limitan las propuestas de desarrollo originadas desde la política pública dirigida a paliar la desigualdad social. Para avanzar en la explicación de estos procesos consideramos necesario: (a) Reconocer las diferentes visiones y modalidades que dan cuenta de la desigualdad social, económica, política e institucional, tanto desde la perspectiva de los actores, como en sus expresiones materiales en el territorio en cuestión. (b) Identificar las relaciones de poder presentes en dicho ámbito (en particular en relación a la disputa por el uso y apropiación del recurso tierra). Y (c) examinar los distintos y contradictorios modelos y propuestas de desarrollo que dichas relaciones expresan. El estudio se basa en una metodología cualitativa sustentada en un estudio de caso y centrada en entrevistas realizadas durante 2008 y 2009 a los principales actores de un conflicto por la tierra situado en el nordeste de Misiones. El trabajo muestra las contradicciones de las políticas públicas que postulan crecimiento productivo y desarrollo en un marco legal de liberalización y desregulación a favor del ingreso de grandes inversiones transnacionales, sustentadas en actividades productivas a gran escala, altamente demandantes de tierra y agua. Inversiones que, contrariamente, tienen un débil requerimiento de fuerza de trabajo. Esta concepción de desarrollo, implícita en la propuesta de crecimiento económico de los sectores que hegemonizan el poder económico, visualiza la desigualdad social como una "externalidad negativa" que, de algún modo debe ser contenida, asistida; desconociendo, ignorando u ocultando que sólo a partir de cambios estructurales es posible superar aquellas desigualdades. En definitiva, se trata de un marco conceptual, ideológico y pragmático que implica un contexto inviable para la agricultura familiar. Por su parte, los pequeños productores y trabajadores rurales que logran alcanzar un mayor grado de comprensión de estos procesos, cuestionan el modelo de desarrollo dominante, así como las alianzas estratégicas que el estado tiene y mantiene con los sectores de poder. Y desde esta perspectiva, luchan por desempeñar un rol en el desarrollo a partir de la producción de alimentos, desde y para su lugar y/o su provincia.

La actividad hidrocarburífera en la Cuenca del Golfo San Jorge : El papel del empresariado regional

La política económica que se implementó en la década de 1990 tuvo como eje, entre las principales medidas, la apertura de la economía, la privatización de las empresas públicas, el desmantelamiento del aparato estatal y sus agentes reguladores, el ajuste fiscal y del gasto público tanto a nivel nacional, como provincial y municipal, la retirada del Estado Nacional como inversor y subsidiador de actividades económicas, la desregulación del mercado de trabajo y la concentración en grupos 'oligopólicos' de las principales actividades productivas, lo que produjo diferentes impactos en las distintas actividades económicas de la Región Patagónica, dependiendo de su inserción en el mercado interno y externo. En este trabajo se pretende, a partir de una descripción de la evolución de la actividad petrolera y de un estudio de caso, analizar los factores que viabilizan el desarrollo económico local y repensar el papel de los actores sociales en relación con el contexto y con las posibilidades de establecimiento de estrategias de innovación en las sociedades locales. En la primera parte del artículo se hace una caracterización socioeconómica de la región de la Cuenca del Golfo San Jorge. A continuación se observa la evolución de la actividad petrolera dentro de la cuenca, su papel en el mercado de trabajo y su requerimiento de mano de obra; se realiza una caracterización de los principales actores y de su relación con el desarrollo local. En la parte final del artículo se intenta mostrar los desafíos que enfrentan los actores públicos y privados vinculados con la actividad petrolera y su proyección en el corto y mediano plazo

De siderúrgica a turística : Breve historia ocupacional de la ciudad de Palpalá (Jujuy-Argentina)

The growth of Palpalá City (Province of Jujuy, argentina) since the 1940s, is linked to altos Hornos Zapla (ahz) the state-owned steel and iron company whose privatization in the early 1990s as part of the external three-sided opening, privatizations and deregulation which characterized convertibility, meant large changes in the local economic structure. In the 1950s, this city was identified by the town hall as 'mother of Industries' ('madre de Industrias'), whereas today, the municipal slogan is 'City of Tourism' ('Ciudad Turística'). Recovering the recent occupational history of this city meets the need to know the labor and socioeconomic reality of this urban area in the Province of Jujuy, about which information is rare. Although it is included in the Home Survey?s sample, it is shown in blocks, making the information very limited. It is paradigmaticin this city to study the means chosen to try to overcome the traumatic shock that ahz's privatization meant, to analyze the policies developed and evaluate their results, some years after their application. In Palpalá, a local development strategy was applied, with a strong impulse to micro-businesses, following, somehow, the model postulated at national level, what will allow us to know the local reality better, as well as the regional and national realities for its characteristics. The methodology used in this work ('From iron and steel industry to tourist city. Brief occupational history of Palpala city) was the bibliographic research of the rare existing studies, interviews to qualified informers as well as the use of secondary data sources, such as data from different national Censuses. To conclude, it can be said that due to the crisis of the 1990s and its state reducing plan, those who suffered its consequences less are, paradoxically, those who are still related to state jobs. In Palpalá, when ahz was privatized and the buyer company failed to fulfill its contract duly, leaving a great number of the local people unemployed, it was the municipal government who had to assume the responsibility of an answer ( in this case, through training, credit and/or counseling to micro-businessmen) In the last years, however, the possibility to work in informal activities has become important in Palpalá, with a high percentage of people working in city fairs selling different kinds of goods. although the change in the model in the recent years has allowed the reactivation in different areas, a preliminary evaluation is that the improvement does not seem to have reached the core of excluded and marginalized of the previous decade

Desarrollo, territorio y desigualdad en la globalización : Conflictos actuales en la agricultura familiar del nordeste de Misiones, Argentina

En el presente trabajo buscamos poner en cuestión la relación entre desarrollo, desigualdad y territorio en el contexto actual de la globalización y en el marco de las políticas públicas dirigidas, supuestamente, a favorecer a los actores sociales de menores recursos. Para ello nos centramos en un estudio de caso en el nordeste de la provincia de Misiones, Argentina. Aquí la actividad tabacalera, la forestación y la agricultura familiar (AF) alternativa (orientada a la producción de alimentos) entran en disputa por el territorio, dando lugar a conflictos por la ocupación de tierras privadas. Su análisis y devenir evidencia: (a) Las transformaciones territoriales resultantes de los procesos de avance de la globalización en ámbitos locales. (b) Los modos en que dichas transformaciones condicionan y limitan las propuestas de desarrollo originadas desde la política pública dirigida a paliar la desigualdad social. Para avanzar en la explicación de estos procesos consideramos necesario: (a) Reconocer las diferentes visiones y modalidades que dan cuenta de la desigualdad social, económica, política e institucional, tanto desde la perspectiva de los actores, como en sus expresiones materiales en el territorio en cuestión. (b) Identificar las relaciones de poder presentes en dicho ámbito (en particular en relación a la disputa por el uso y apropiación del recurso tierra). Y (c) examinar los distintos y contradictorios modelos y propuestas de desarrollo que dichas relaciones expresan. El estudio se basa en una metodología cualitativa sustentada en un estudio de caso y centrada en entrevistas realizadas durante 2008 y 2009 a los principales actores de un conflicto por la tierra situado en el nordeste de Misiones. El trabajo muestra las contradicciones de las políticas públicas que postulan crecimiento productivo y desarrollo en un marco legal de liberalización y desregulación a favor del ingreso de grandes inversiones transnacionales, sustentadas en actividades productivas a gran escala, altamente demandantes de tierra y agua. Inversiones que, contrariamente, tienen un débil requerimiento de fuerza de trabajo. Esta concepción de desarrollo, implícita en la propuesta de crecimiento económico de los sectores que hegemonizan el poder económico, visualiza la desigualdad social como una "externalidad negativa" que, de algún modo debe ser contenida, asistida; desconociendo, ignorando u ocultando que sólo a partir de cambios estructurales es posible superar aquellas desigualdades. En definitiva, se trata de un marco conceptual, ideológico y pragmático que implica un contexto inviable para la agricultura familiar. Por su parte, los pequeños productores y trabajadores rurales que logran alcanzar un mayor grado de comprensión de estos procesos, cuestionan el modelo de desarrollo dominante, así como las alianzas estratégicas que el estado tiene y mantiene con los sectores de poder. Y desde esta perspectiva, luchan por desempeñar un rol en el desarrollo a partir de la producción de alimentos, desde y para su lugar y/o su provincia.

Desarrollo, territorio y desigualdad en la globalización : Conflictos actuales en la agricultura familiar del nordeste de Misiones, Argentina

En el presente trabajo buscamos poner en cuestión la relación entre desarrollo, desigualdad y territorio en el contexto actual de la globalización y en el marco de las políticas públicas dirigidas, supuestamente, a favorecer a los actores sociales de menores recursos. Para ello nos centramos en un estudio de caso en el nordeste de la provincia de Misiones, Argentina. Aquí la actividad tabacalera, la forestación y la agricultura familiar (AF) alternativa (orientada a la producción de alimentos) entran en disputa por el territorio, dando lugar a conflictos por la ocupación de tierras privadas. Su análisis y devenir evidencia: (a) Las transformaciones territoriales resultantes de los procesos de avance de la globalización en ámbitos locales. (b) Los modos en que dichas transformaciones condicionan y limitan las propuestas de desarrollo originadas desde la política pública dirigida a paliar la desigualdad social. Para avanzar en la explicación de estos procesos consideramos necesario: (a) Reconocer las diferentes visiones y modalidades que dan cuenta de la desigualdad social, económica, política e institucional, tanto desde la perspectiva de los actores, como en sus expresiones materiales en el territorio en cuestión. (b) Identificar las relaciones de poder presentes en dicho ámbito (en particular en relación a la disputa por el uso y apropiación del recurso tierra). Y (c) examinar los distintos y contradictorios modelos y propuestas de desarrollo que dichas relaciones expresan. El estudio se basa en una metodología cualitativa sustentada en un estudio de caso y centrada en entrevistas realizadas durante 2008 y 2009 a los principales actores de un conflicto por la tierra situado en el nordeste de Misiones. El trabajo muestra las contradicciones de las políticas públicas que postulan crecimiento productivo y desarrollo en un marco legal de liberalización y desregulación a favor del ingreso de grandes inversiones transnacionales, sustentadas en actividades productivas a gran escala, altamente demandantes de tierra y agua. Inversiones que, contrariamente, tienen un débil requerimiento de fuerza de trabajo. Esta concepción de desarrollo, implícita en la propuesta de crecimiento económico de los sectores que hegemonizan el poder económico, visualiza la desigualdad social como una "externalidad negativa" que, de algún modo debe ser contenida, asistida; desconociendo, ignorando u ocultando que sólo a partir de cambios estructurales es posible superar aquellas desigualdades. En definitiva, se trata de un marco conceptual, ideológico y pragmático que implica un contexto inviable para la agricultura familiar. Por su parte, los pequeños productores y trabajadores rurales que logran alcanzar un mayor grado de comprensión de estos procesos, cuestionan el modelo de desarrollo dominante, así como las alianzas estratégicas que el estado tiene y mantiene con los sectores de poder. Y desde esta perspectiva, luchan por desempeñar un rol en el desarrollo a partir de la producción de alimentos, desde y para su lugar y/o su provincia.

La actividad hidrocarburífera en la Cuenca del Golfo San Jorge : El papel del empresariado regional

La política económica que se implementó en la década de 1990 tuvo como eje, entre las principales medidas, la apertura de la economía, la privatización de las empresas públicas, el desmantelamiento del aparato estatal y sus agentes reguladores, el ajuste fiscal y del gasto público tanto a nivel nacional, como provincial y municipal, la retirada del Estado Nacional como inversor y subsidiador de actividades económicas, la desregulación del mercado de trabajo y la concentración en grupos 'oligopólicos' de las principales actividades productivas, lo que produjo diferentes impactos en las distintas actividades económicas de la Región Patagónica, dependiendo de su inserción en el mercado interno y externo. En este trabajo se pretende, a partir de una descripción de la evolución de la actividad petrolera y de un estudio de caso, analizar los factores que viabilizan el desarrollo económico local y repensar el papel de los actores sociales en relación con el contexto y con las posibilidades de establecimiento de estrategias de innovación en las sociedades locales. En la primera parte del artículo se hace una caracterización socioeconómica de la región de la Cuenca del Golfo San Jorge. A continuación se observa la evolución de la actividad petrolera dentro de la cuenca, su papel en el mercado de trabajo y su requerimiento de mano de obra; se realiza una caracterización de los principales actores y de su relación con el desarrollo local. En la parte final del artículo se intenta mostrar los desafíos que enfrentan los actores públicos y privados vinculados con la actividad petrolera y su proyección en el corto y mediano plazo

De siderúrgica a turística : Breve historia ocupacional de la ciudad de Palpalá (Jujuy-Argentina)

The growth of Palpalá City (Province of Jujuy, argentina) since the 1940s, is linked to altos Hornos Zapla (ahz) the state-owned steel and iron company whose privatization in the early 1990s as part of the external three-sided opening, privatizations and deregulation which characterized convertibility, meant large changes in the local economic structure. In the 1950s, this city was identified by the town hall as 'mother of Industries' ('madre de Industrias'), whereas today, the municipal slogan is 'City of Tourism' ('Ciudad Turística'). Recovering the recent occupational history of this city meets the need to know the labor and socioeconomic reality of this urban area in the Province of Jujuy, about which information is rare. Although it is included in the Home Survey?s sample, it is shown in blocks, making the information very limited. It is paradigmaticin this city to study the means chosen to try to overcome the traumatic shock that ahz's privatization meant, to analyze the policies developed and evaluate their results, some years after their application. In Palpalá, a local development strategy was applied, with a strong impulse to micro-businesses, following, somehow, the model postulated at national level, what will allow us to know the local reality better, as well as the regional and national realities for its characteristics. The methodology used in this work ('From iron and steel industry to tourist city. Brief occupational history of Palpala city) was the bibliographic research of the rare existing studies, interviews to qualified informers as well as the use of secondary data sources, such as data from different national Censuses. To conclude, it can be said that due to the crisis of the 1990s and its state reducing plan, those who suffered its consequences less are, paradoxically, those who are still related to state jobs. In Palpalá, when ahz was privatized and the buyer company failed to fulfill its contract duly, leaving a great number of the local people unemployed, it was the municipal government who had to assume the responsibility of an answer ( in this case, through training, credit and/or counseling to micro-businessmen) In the last years, however, the possibility to work in informal activities has become important in Palpalá, with a high percentage of people working in city fairs selling different kinds of goods. although the change in the model in the recent years has allowed the reactivation in different areas, a preliminary evaluation is that the improvement does not seem to have reached the core of excluded and marginalized of the previous decade

The Sheepskin Marketing Channel in Ethiopia after Liberalization: The Survival Strategies of the Participants

Sheepskin market in Ethiopia seems to enjoy the benefits of the economic liberalization policy since 1991, such as price deregulation or liberalization of economic activities. The close observation on the profiles and activities of each actor in the marketing channel, however, illustrates that the actors have tried to complement the lack of appropriate infrastructure and legal protection, which should be provided by the government, through utilizing their social and blood ties. While these ties have implemented the role of the government, there are risks that the marketing channel might be developed based on coalition with social network and the poor without the network would be excluded.

A Flowchart Approach to Malaysia's Automobile Industry Cluster Policy

In this paper, we apply a flowchart approach to investigate Malaysia's automobile cluster policy. We investigate whether the industrial cluster policy has been successful or not, suggest policy prescriptions, and propose a way to prioritize policy measures. Our flowchart approach leads to the following three policy prescriptions: (1) Malaysian firms should establish sites for exporting compact cars with automatic transmissions; (2) actors in the public, semi-public and private sector should work to upgrade skilled labor; and (3) the central government should promote liberalization and deregulation to attract foreign firms into the supporting industries.