Vertical distribution of relative numbers of the main groups of sound-scattering fishes in the Central Indian Ocean

Findings made in 31 catches with an Isaacs-Kidd midwater trawl in the light (09.00-16.00) and dark (21.00-04.00) periods of a day within a survey area of about 100 sq. miles with approximate center coordinates of 13°S and 78°E have been used to investigate vertical distribution of the main groups of sound-scattering fishes (35 species of the family Myctophidae and 16 species of other families). It has been shown that during daylight hours all fishes sink to depths deeper than 400 m. Data are presented concerning the fish population of night-time sound-scattering layers at depths of 70-150 m and about 400 m and of the daytime ones at depths of about 450 m.

Near-bottom zooplankton aggregations in Kongsfjorden with link to images from the optical zooplankton sensor MOKI

Near-bottom zooplankton communities have rarely been studied despite numerous reports of high zooplankton concentrations, probably due to methodological constraints. In Kongsfjorden, Svalbard, the near-bottom layer was studied for the first time by combining daytime deployments of a remotely operated vehicle (ROV), the optical zooplankton sensor moored on-sight key species investigation (MOKI), and Tucker trawl sampling. ROV data from the fjord entrance and the inner fjord showed high near-bottom abundances of euphausiids with a mean concentration of 17.3 ± 3.5 n/100 m**3. With the MOKI system, we observed varying numbers of euphausiids, amphipods, chaetognaths, and copepods on the seafloor at six stations. Light-induced zooplankton swarms reached densities in the order of 90,000 (euphausiids), 120,000 (amphipods), and 470,000 ind/m**3 (chaetognaths), whereas older copepodids of Calanus hyperboreus and C. glacialis did not respond to light. They were abundant at the seafloor and 5 m above and showed maximum abundance of 65,000 ind/m**3. Tucker trawl data provided an overview of the seasonal vertical distribution of euphausiids. The most abundant species Thysanoessa inermis reached near-bottom concentrations of 270 ind/m**3. Regional distribution was neither related to depth nor to location in the fjord. The taxa observed were all part of the pelagic community. Our observations suggest the presence of near-bottom macrozooplankton also in other regions and challenge the current view of bentho-pelagic coupling. Neglecting this community may cause severe underestimates of the stock of elagic zooplankton, especially predatory species, which link secondary production with higher trophic levels.

A database of multi-year (2004-2010) quality-assured surface solar hourly irradiation measurements for the Egyptian territory

A database containing the global and diffuse components of the surface solar hourly irradiation measured from 1 January 2004 to 31 December 2010 at eight stations of the Egyptian Meteorological Authority is presented. For three of these sites (Cairo, Aswan, and El-Farafra), the direct component is also available. In addition, a series of meteorological variables including surface pressure, relative humidity, temperature, wind speed and direction is provided at the same hourly resolution at all stations. The details of the experimental sites and instruments used for the acquisition are given. Special attention is paid to the quality of the data and the procedure applied to flag suspicious or erroneous measurements is described in details. Between 88 and 99% of the daytime measurements are validated by this quality control. Except at Barrani where the number is lower (13500), between 20000 and 29000 measurements of global and diffuse hourly irradiation are available at all sites for the 7-year period. Similarly, from 9000 to 13000 measurements of direct hourly irradiation values are provided for the three sites where this component is measured. With its high temporal resolution this consistent irradiation and meteorological database constitutes a reliable source to estimate the potential of solar energy in Egypt. It is also adapted to the study of high-frequency atmospheric processes such as the impact of aerosols on atmospheric radiative transfer. In the next future, it is planned to complete regularly the present 2004-2010 database.

Cacao trees in tropical agroforestry landscapes of the Napu Valley in Central Sulawesi, Indonesia

All sample sites were situated at the northern tip of Napu Valley in Central Sulawesi, Indonesia. After an initial mapping of the study area, we selected 15 smallholder cacao plantations as sites for bird and bat exclosure experiments in March 2010. On each study site, we established 4 treatments for these exclosure experiments (bird exclosure - closed during daytime and open during night; bat exclosure - closed overnight and opened during daytime; full exclosure of both birds and bats - always closed and unmanipulated/open control treatments - always open). In each treatment, there were 2 cacao trees (total of 8 cacao trees per study site), surrounded by nylon filament (2x2 cm mesh size) that was opened and closed according to the activity period of day and night active flying vertebrates (05:00-06:00 am and 17:00-18:00 pm) on a daily basis. The mean tree height and diameter at breast height (dbh) result from two measures of all study trees at the beginning of the exclosure experiment (June 2010) and 6 months later (February 2011).

Distribution of macrozooplankton at time series station MIGZOO-4

A large population of the colonial pelagic tunicate Pyrosoma atlanticum occurred in April 1991 in offshore waters of the Ligurian Sea (Northwestern Mediterranean). The high numbers of colonies caught allowed their vertical distribution and diel migration in the 0-965 m water column to be described as a function of their size. Daytime depths and amplitudes of the migration were correlated with colony size. The amplitude of the migration ranged from 90 m for 3-mm-length colonies to 760 m for 51-mm-length colonies, with a mean amplitude of 410 m for the whole population, all sizes pooled. The results of horizontal hauls at a given depth around sunrise and sunset showed a marked diurnal symmetry of the migratory cycle relative to noon, and that migration of the population was not cohesive. For example, the larger the colonies, the later after sunset they reached the upper layers during their upward migration.

Dive depth and dive duration data of female southern elephant seals from Marion Island between 2004 and 2008 with links to datasets

The at-sea behaviour of marine top predators provides valuable insights into the distribution of prey species and strategies used by predators to exploit patchily distributed resources. We describe the water column usage and dive strategies of female southern elephant seals from Marion Island tracked between 2004 and 2008. Dives representing increases in forage effort were identified using a method that combines dive type analyses and the calculation of relative amounts of time that animals spend in the bottom phases of dives. Results from this analysis indicate that female elephant seals from Marion Island tend to display lower levels of forage effort closer to the island and display intensive opportunistic forage bouts that occur at a minimum distance of approximately 215 km from the island. Females from Marion Island dived deeper and for longer periods of time, compared to females from other populations. Most animals displayed positive diel vertical migration, evidently foraging pelagically on vertically migrating prey. A few animals displayed periods of reverse (negative) diel vertical migration, however, diving to deeper depths at night, compared to daytime. This behaviour is difficult to explain and prey species targeted during such periods unknown. Our results illustrate plasticity in foraging behaviour of southern elephant seals, as well as inter-population differences in forage strategies.

Feeding of Sagitta setosa in the Black Sea

Gut dissection of fixed individuals from samples collected during Cruise 6 of R/V Vityaz-2 in April-May 1984 was used to study feeding of Sagitta setosa in the layers of daytime plankton accumulation at the lower boundary of the oxycline. The principal food was copepodite stage V of Calanus and females of Calanus and Pseudocalanus. Analysis of daytime and night data with reference to length of migratory alterations of Sagitta populations and gut passage time indicates that they feed actively in the layers of day¬time plankton accumulations. Total food consumption during time spent in the subsurface layers ranged from 0.025-0.097 cal/indiv. in 12 h, equivalent to 37-143% of their metabolic energy expenditure. Over the course of 12 h Sagitta population consumes 0.3-5% and 0.5-6% of population of stage V copepodites and females of Calanus and Pseudocalanus females, respectively.

Dive depth profile and immobilisation data of adult male and female Weddell seals at Drescher Inlet from expedition DRE1998

Adult male and female Weddell seals (Leptonychotes weddellii) were fitted with Time-depth recorders (TDR) at Drescher Inlet (Riiser Larsen Ice Shelf), eastern Weddell Sea coast, in February 1998. Eight of 15 data sets were selected for analyses to investigate the seals' foraging behaviour (doi:10.1594/PANGAEA.511465, doi:10.1594/PANGAEA.511454, doi:10.1594/PANGAEA.511456, doi:10.1594/PANGAEA.511457, doi:10.1594/PANGAEA.511459, doi:10.1594/PANGAEA.511462, doi:10.1594/PANGAEA.511466, doi:10.1594/PANGAEA.511467). These data sets provided simultaneous dive records of eight seals over eight days. The seals primarily foraged within two depth layers, these being from the sea surface to 160 m where temperature and salinity varied considerably, and from 340 to 450 m near the bottom where temperature was lowest and salinity highest, with little variation. While pelagic and benthic diving occurred during daylight, the seals foraged almost exclusively in the upper water column at night. Trawling during daytime confirmed that Pleuragramma antarcticum were by far the most abundant fish both in the pelagial and close to the bottom. Pelagic night-hauls at 110-170 m depth showed highly variable biomass of P. antarcticum with a peak at around midnight. The temporal changes in the local abundance of P. antarcticum, particularly in the pelagial, may explain the trends in the seals' pelagic and benthic foraging activities. This is the first study which describes the jaw movements of a hunting seal which are presumably indicative of feeding events. Trophic links from the Weddell seal to fish, zooplankton and krill, Euphausia superba, are discussed. Another seven data sets did not overlap substantially with the selected time frame (doi:10.1594/PANGAEA.511458, doi:10.1594/PANGAEA.511460, doi:10.1594/PANGAEA.511464, doi:10.1594/PANGAEA.511468, doi:10.1594/PANGAEA.511469, doi:10.1594/PANGAEA.511453, doi:10.1594/PANGAEA.511463). A total of 25 Weddell seals were immobilised during the study period using a combination of ketamine, xylazine, and diazepam. Seven seals were drugged once, 15 seals two times, and three were drugged three times, coming to a total of 46 immobilisation procedures. Narcoses were terminated with yohimbine (doi:10.1594/PANGAEA.438933).

Seawater carbonate chemistry and concentration boundary layers around complex assemblages of macroalgae in a laboratory experiment

Metabolic processes have the potential to modulate the effects of ocean acidification (OA) in nearshore macroalgal beds. We investigated whether natural mixed assemblages of the articulate coralline macroalgae Arthrocardia corymbosa and understory crustose coralline algae (CCA) altered pH and O2 concentrations within and immediately above their canopies. In a unidirectional flume, we tested the effect of water velocity (0-0.1 m/s), bulk seawater pH (ambient pH 8.05, and pH 7.65), and irradiance (photosynthetically saturating light and darkness) on pH and O2 concentration gradients, and the derived concentration boundary layer (CBL) thickness. At bulk seawater pH 7.65 and slow velocities (0 and 0.015 m/s), pH at the CCA surface increased to 7.90-8.00 in the light. Although these manipulations were short term, this indicates a potential daytime buffering capacity that could alleviate the effects of OA. Photosynthetic activity also increased O2 concentrations at the surface of the CCA. However, this moderating capacity was flow dependent; the CBL thickness decreased from an average of 26.8 mm from the CCA surface at 0.015 m/s to 4.1 mm at 0.04 m/s. The reverse trends occurred in the dark, with respiration causing pH and O2 concentrations to decrease at the CCA surface. At all flow velocities the CBL thicknesses (up to 68 mm) were much greater than those previously published, indicating that the presence of canopies can alter the CBL substantially. In situ, the height of macroalgal canopies can be an order of magnitude larger than those used here, indicating that the degree of buffering to OA will be context dependent.

Coral-algae metabolism and diurnal changes in the CO2-carbonate system of bulk sea water

Precise measurements were conducted in continuous flow seawater mesocosms located in full sunlight that compared metabolic response of coral, coral-macroalgae and macroalgae systems over a diurnal cycle. Irradiance controlled net photosynthesis (Pnet), which in turn drove net calcification (Gnet), and altered pH. Pnet exerted the dominant control on [CO3]2- and aragonite saturation state (Omega arag) over the diel cycle. Dark calcification rate decreased after sunset, reaching zero near midnight followed by an increasing rate that peaked at 03:00 h. Changes in Omega arag and pH lagged behind Gnet throughout the daily cycle by two or more hours. The flux rate Pnet was the primary driver of calcification. Daytime coral metabolism rapidly removes dissolved inorganic carbon (DIC) from the bulk seawater and photosynthesis provides the energy that drives Gnet while increasing the bulk water pH. These relationships result in a correlation between Gnet and Omega arag, with Omega arag as the dependent variable. High rates of H+ efflux continued for several hours following mid-day peak Gnet suggesting that corals have difficulty in shedding waste protons as described by the Proton Flux Hypothesis. DIC flux (uptake) followed Pnet and Gnet and dropped off rapidly following peak Pnet and peak Gnet indicating that corals can cope more effectively with the problem of limited DIC supply compared to the problem of eliminating H+. Over a 24 h period the plot of total alkalinity (AT) versus DIC as well as the plot of Gnet versus Omega arag revealed a circular hysteresis pattern over the diel cycle in the coral and coral-algae mesocosms, but not the macroalgae mesocosm. Presence of macroalgae did not change Gnet of the corals, but altered the relationship between Omega arag and Gnet. Predictive models of how future global changes will effect coral growth that are based on oceanic Omega arag must include the influence of future localized Pnet on Gnet and changes in rate of reef carbonate dissolution. The correlation between Omega arag and Gnet over the diel cycle is simply the response of the CO2-carbonate system to increased pH as photosynthesis shifts the equilibria and increases the [CO3]2- relative to the other DIC components of [HCO3]- and [CO2]. Therefore Omega arag closely tracked pH as an effect of changes in Pnet, which also drove changes in Gnet. Measurements of DIC flux and H+ flux are far more useful than concentrations in describing coral metabolism dynamics. Coral reefs are systems that exist in constant disequilibrium with the water column.

Seawater carbonate chemistry and auditory behavior of Orange clownfish (Amphiprion percula) during experiments, 2011

Ocean acidification is predicted to affect marine ecosystems in many ways, including modification of fish behaviour. Previous studies have identified effects of CO2-enriched conditions on the sensory behaviour of fishes, including the loss of natural responses to odours resulting in ecologically deleterious decisions. Many fishes also rely on hearing for orientation, habitat selection, predator avoidance and communication. We used an auditory choice chamber to study the influence of CO2-enriched conditions on directional responses of juvenile clownfish (Amphiprion percula) to daytime reef noise. Rearing and test conditions were based on Intergovernmental Panel on Climate Change predictions for the twenty-first century: current-day ambient, 600, 700 and 900 µatm pCO2. Juveniles from ambient CO2-conditions significantly avoided the reef noise, as expected, but this behaviour was absent in juveniles from CO2-enriched conditions. This study provides, to our knowledge, the first evidence that ocean acidification affects the auditory response of fishes, with potentially detrimental impacts on early survival.

Effect of ocean acidification and pH fluctuations on the growth and development of coralline algal recruits, and an associated benthic algal assemblage

Coralline algae are susceptible to the changes in the seawater carbonate system associated with ocean acidification (OA). However, the coastal environments in which corallines grow are subject to large daily pH fluctuations which may affect their responses to OA. Here, we followed the growth and development of the juvenile coralline alga Arthrocardia corymbosa, which had recruited into experimental conditions during a prior experiment, using a novel OA laboratory culture system to simulate the pH fluctuations observed within a kelp forest. Microscopic life history stages are considered more susceptible to environmental stress than adult stages; we compared the responses of newly recruited A. corymbosa to static and fluctuating seawater pH with those of their field-collected parents. Recruits were cultivated for 16 weeks under static pH 8.05 and 7.65, representing ambient and 4*preindustrial pCO2 concentrations, respectively, and two fluctuating pH treatments of daily (daytime pH = 8.45, night-time pH = 7.65) and daily (daytime pH = 8.05, night-time pH = 7.25). Positive growth rates of new recruits were recorded in all treatments, and were highest under static pH 8.05 and lowest under fluctuating pH 7.65. This pattern was similar to the adults' response, except that adults had zero growth under fluctuating pH 7.65. The % dry weight of MgCO3 in calcite of the juveniles was reduced from 10% at pH 8.05 to 8% at pH 7.65, but there was no effect of pH fluctuation. A wide range of fleshy macroalgae and at least 6 species of benthic diatoms recruited across all experimental treatments, from cryptic spores associated with the adult A. corymbosa. There was no effect of experimental treatment on the growth of the benthic diatoms. On the community level, pH-sensitive species may survive lower pH in the presence of diatoms and fleshy macroalgae, whose high metabolic activity may raise the pH of the local microhabitat.