997 resultados para Cicloni,Climatologia,Mar Egeo,sistemi a bassa pressione,attività ciclonica,ERA5
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El document descriu com el just-in-time va afavorir la fallida dels sistemes productius de les empreses d'automoció al Japó durant el gran terratrèmol de l'any 2011.
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En els treballs de Mar Arza es percep una preocupació sostinguda per les línies escrites queapareixen sobre les superfícies. En els inicis, alterava les pàgines i les lletres dels llibres de vell. Recentment, ha intervingut la lletra escrita i els buits de qualsevol paper imprès –llibretes d’estalvis, factures de la llum, volants mèdics, fulls de calendaris, dècims de loteria...
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En los trabajos de Mar Arza se percibe una preocupación sostenida por las líneas escritasque aparecen sobre las superficies. En los inicios, alteraba las páginas y las letras de los librosde viejo. Recientemente, ha intervenido la letra escrita y los vacíos de cualquier papel impreso–libretas de ahorros, facturas de la luz, volantes médicos, hojas de calendarios, décimos de lotería...
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In the works of Mar Arza we perceive a sustained concern with lines of writing on surfaces. Earlyon, she altered the pages and letters of old books. More recently she has intervened in the lettersand the blank spaces on all kinds of printed paper — savings books, electricity bills, medical referrals, calendar pages, lottery tickets...
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Informe sobre les publicacions de recerca de la UOC introduides a ISI Web of Knowledge durant
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Lo nuevo hoy no es la inseguridad de los ciudadanos. Lo nuevo hoy es plantearse los problemas derivados de la inseguridad turística como un reto más a añadir a los que ya se enfrentan los planificadores del turismo, y no como una asignatura independiente sino integrada en la estrategia de desarrollo global y sostenible de los destinos.
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The untargeted integration of foreign DNA into the mammalian cell genome, extensively used in gene therapy and biotechnology, remains an incompletely understood process. It is believed to be based on cellular DNA double strand break (DSB) repair machinery and to involve two major steps: i) the formation of long gene arrays (concatemers), and ii) recombination of the resulting concatemer with the genome. The main DSB repair pathways in eukaryotes include non-homologous end-joining (NHEJ), homologous recombination (HR), and microhomology-mediated end-joining (MMEJ). However, it is still not clear, which of these pathways are responsible for transgene integration. Here, we show that NHEJ is not the primary pathway used by mammalian cells in the transgene integration process, while the components of the HR pathway seem to be important for genomic integration but not concatemerization. Instead, concatemer formation appears to be mediated by a subset of the MMEJ pathway, termed synthesis-dependent MMEJ (SD-MMEJ). This mechanism also seems to be preferentially used for plasmid integration into the genome, as confirmed by the analysis of plasmid-to-genome junction sequences, which were found to display an SD-MMEJ pattern. Therefore, we propose the existence of two distinct SD-MMEJ subpathways, relying on different subsets of enzymes. One of these mechanisms appears to be responsible for concatemerization, while the other mechanism, partially dependent in HR enzymes, seems to mediate recombination with the genome. Previous studies performed by our group suggested that matrix attachment regions (MARs), which are epigenetic regulatory DNA elements that participate in the formation of chromatin boundaries and augment transcription, may mediate increased plasmid integration into the genome of CHO cells by stimulating DNA recombination. In the present work, we demonstrate that MAR-mediated plasmid integration results from the enhanced SD-MMEJ pathway. Analysis of transgene integration loci and junction DNA sequences validated the prevalent use of this pathway by the MAR elements to target plasmid DNA into gene-rich areas of the CHO genome. We propose that this finding should in the future help to engineer cells for improved recombinant protein production. In addition to investigating the process of transgene integration, we designed recombination assays to better characterize the components of the MMEJ and SD-MMEJ pathways. We also used CHO cells expressing cycle-sensitive reporter genes to demonstrate a potential role of HR proteins in the cell cycle regulation.
