Presence of antibodies to different subunits of replication protein A in autoimmune sera.

A human cDNA expression library was used to investigate the nature of molecules recognized by serum from a patient with Sjögren syndrome that exhibits a mixed immunofluorescence pattern and reacts with multiple components on an immunoblot. The data demonstrated that this serum contains IgG antibodies specific for the 70- and 32-kDa subunits of replication protein A (RPA; RPA-70 and RPA-32, respectively), a highly conserved multisubunit DNA binding protein. Affinity purification of serum autoantibodies demonstrated a complete lack of cross-reactivity between RPA-70 and RPA-32, suggesting a direct participation of the native protein complex in the autoimmune response in this patient. Purified anti-RPA-70 and anti-RPA-32 antibodies labeled nuclear and cytoplasmic components in an immunofluorescence assay, suggesting that RPA is present in both cellular compartments. Additional sera from 55 patients with different autoimmune conditions were screened against purified RPA-70 and RPA-32 recombinant proteins. One of these 55 sera was positive and reacted with only RPA-32. Twenty sera from healthy control individuals did not react with RPA. These results show that RPA is a target for autoantibodies in human autoimmune diseases, although its precise frequency, occurrence in other autoimmune diseases, and pathological significance remain to be fully elucidated.

Construction of Intelligent Virtual Worlds Using a Grammatical Framework

The potential of integrating multiagent systems and virtual environments has not been exploited to its whole extent. This paper proposes a model based on grammars, called Minerva, to construct complex virtual environments that integrate the features of agents. A virtual world is described as a set of dynamic and static elements. The static part is represented by a sequence of primitives and transformations and the dynamic elements by a series of agents. Agent activation and communication is achieved using events, created by the so-called event generators. The grammar defines a descriptive language with a simple syntax and a semantics, defined by functions. The semantics functions allow the scene to be displayed in a graphics device, and the description of the activities of the agents, including artificial intelligence algorithms and reactions to physical phenomena. To illustrate the use of Minerva, a practical example is presented: a simple robot simulator that considers the basic features of a typical robot. The result is a functional simple simulator. Minerva is a reusable, integral, and generic system, which can be easily scaled, adapted, and improved. The description of the virtual scene is independent from its representation and the elements that it interacts with.

Letter from William Tudor to his family,

Four letters written from Tudor to his family while he was traveling in France in 1800 and 1802. In two letters, he describes in detail his capture by a French privateer aboard the ship Minerva in the spring of 1800. He also discusses the business activities of "Mr. C" (John Codman), his employer. Tudor additionally describes the French countryside and the impact of the French Revolutionary Wars on the cities.

Senderos.

Poems.

Literatura chilena.

Includes bibliography.

Bosquejo histórico de la literatura chilena. Período colonial.

Cover imprint: Santiago de Chile, Librería y casa editorial "Minerva."

La cosecha de otoño.

Cover imprint: Santiago - Chile, "Minerva".

Cabezas : siluetas de políticos, literatos, diplomáticos y otras liendres de la fauna pintoresca /

"... art iculos publicados en 'El diario' con el pseudónimo de 'Nolo Beas', ..."--Nota de los editores.

Solennizzandosi il giorno XII agosto MDCCCXLIV nel convento delle Clarisse in Lovere la professione di suor Maria Candida Rosa Molinari : canzone : dedicata al merito esimio del signore Giovita Cantoni.

Acquisition made accessible thanks to a 2015-2017 grant from the Council on Libraries and Information Resources.

Pronunciando i voti solenni suor Maria Candida Molinari nel monastero delle Clarisse in Lovere : sonetto : dedicato al merito esimio del molto rev. signore don Pietro Superti, degnissimo rettore del Santuario delle Grazie in Brescia.

Acquisition made accessible thanks to a 2015-2017 grant from the Council on Libraries and Information Resources.

Alsino /

Mode of access: Internet.

Violines y toneles.

Imprint covered by label.

Crónicas.

Imprint on cover: Editorial Minerva.

Como si fuera ayer! /

Essays.