Soil loss risk and habitat quality in streams of a meso-scale river basin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Infestação por ancilostomídeos e toxocarídeos em cães e gatos apreendidos em vias públicas, São Paulo (Brasil)

Foram examinadas fezes, coletadas após o sacrifício, de 9.150 cães e 674 gatos, capturados nas vias públicas do Município de São Paulo, SP (Brasil), visando o encontro de ovos e parásitos das famílias Ancylostomidae e Ascaridae. A coleta do material ocorreu durante os anos de 1980-1985. As seguintes taxas de infestação foram observadas: 59,83% dos cães e 22,26% dos gatos estiveram positivos para ovos de Ancylostoma sp., enquanto que 11,70% dos cães e 17,65% dos gatos apresentaram ovos de Toxocara sp.

Resistividade do filme depositado via sol-gel e estado de oxidação do dopante Ce na matriz SnO2

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

O licenciamento ambiental da mineração no Quadrilátero Ferrífero de Minas Gerais: uma análise da implementação de medidas de controle ambiental formuladas em EIAs/RIMAs

Instrumento de política pública ligado ao licenciamento ambiental prévio de projetos de empreendimentos potencialmente degradadores do ambiente, a Avaliação de Impacto Ambiental (AIA), atualmente, apresenta algumas inconsistências. Dentre elas, autores registram a disparidade no que se refere à indicação e à efetiva implantação das medidas de controle ambiental estabelecidas nos EIA/RIMAs. Diante disso, o presente trabalho analisa a implantação das medidas mitigadoras estabelecidas para empreendimentos minerais licenciados pela Fundação Estadual do Meio Ambiente (FEAM) do Estado de Minas Gerais, para a região do Quadrilátero Ferrífero. No estudo, constata-se que intervenções ambientais são hoje partes integrantes dos projetos minerais; porém, como na mineração a operação impõe certo dinamismo ao empreendimento, verifica-se que parte das medidas mitigadoras apontadas no EIA acaba não sendo executada, como o previsto e o aprovado na fase da Licença Prévia (LP), enquanto outras apenas ficam listadas como propostas de mitigação de impactos, deixando de ser efetivamente executadas.

Época de sobressemeadura de gramíneas anuais de inverno e de verão no capim-tifton 85: valor nutritivo

Conduziu-se o experimento na UNESP-Jaboticabal, no período de inverno-primavera-verão de 2001-2002, objetivando determinar os teores de proteína bruta (PB), parede celular (FDN, FDA, lignina, celulose e hemicelulose) e a digestibilidade in vitro da matéria orgânica (DIVMO) no capim-Tifton 85 exclusivo e sobressemeado com aveia preta, milheto e sorgo-sudão. Os tratamentos testados foram: milheto+aveia preta; AG2501C+aveia preta, sobressemeados nas áreas de Tifton-85, em 19-06 ou 02-07-2002, e capim-Tifton 85 (Testemunha). O delineamento experimental foi de blocos casualizados, com três repetições. Os teores de PB foram maiores na primeira avaliação quando comparada às demais. Observou-se aumento nos teores de FDN, FDA e lignina, e comportamento inverso nos valores de DIVMO, em relação aos períodos de crescimento, nas duas épocas de semeadura. Os teores avaliados de PB, parede celular e DIVMO apresentaram variação em função da composição botânica e morfológica, no decorrer das avaliações e épocas de semeadura.

Poaia [Psychotria ipecacuanha (Brot.) Stoves]: aspectos da memória cultural dos poaieiros de Cáceres - Mato Grosso, Brasil

O Brasil está entre os principais exportadores de poaia [Psychotria ipecacuanha (Brot.) Stoves] seguido do Panamá e Costa Rica. A poaia brasileira apresenta alto valor farmacológico das raízes devido aos teores de emetina e cefalina. Este trabalho teve como objetivo descrever como as famílias de poaieiros mantém a memória cultural sobre a Psychotria ipecacuanha (Brot.) Stoves. As informações foram coletadas no município de Cáceres, Mato Grosso, através de entrevista estruturada e observação participante com 20 homens e 10 mulheres, de faixa etária de 45 a 86 anos. Foram citadas as formas de utilização na alimentação para animais, inseticida, carrapaticida, emético, contra diarréias, para alívio de dor de cabeça, contra malária, bronquite e dor no estômago. A raiz é a parte mais usada e a forma de preparo é tintura ou misturada ao fumo, ao vinho ou à cachaça. Poucos entrevistados passaram aos filhos o conhecimento sobre a P. ipecacuanha. A memória cultural sobre a P. ipecacuanha deve-se a vivência, extração e comercialização da planta, e por ouvir as conversas dos pais com amigos. A perda de conhecimento associado a poaia é causada pelo êxodo rural, destruição do habitat com o desmatamento e ocupação agrícola. A extinção da espécie na região contribui para a erosão cultural.

Evaluation of rare earth oxides doping SnO2.(Co1/4,Mn3/4)O-based varistor system

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sistemas de Alimentação com Livre Escolha e Semi Livre Escolha para Poedeiras

This research was carried out to verify the ability of laying hens to select food in order to meet their requirements for protein and energy. Ninety-six Hy-Line White laying hens, 45 weeks old, were allotted to a randomized block design with two blocks (two ranges of body weight), four treatments, and three replicates of four hens in each block. The treatments consisted of four different feeding systems: I-Conventional feeding represented by a complete ration composed of 60% ground corn and 40% protein concentrate; 2-Free-choice feeding with ground corn, protein concentrate and oyster shells fed in feeders with three separate compartments; 3-Semi free-choice feeding with whole corn grain, protein concentrate and oyster shells in the same proportion in one feeder; 4-Free-choice feeding with whole corn grain, protein concentrate and oyster shells fed in feeders with three separate compartments. The results indicated that the hens, in spite of age, adapt to different feeding systems, and that they can select feed to meet their nutrient requirements. The semi free-choice and free-choice feeding systems with whole corn grain resulted in the same performance compared to conventional feeding, but shell quality was not improved by oyster shell supplementation. However, the worst performance was with free-choice feeding with ground corn, which indicated that in the free-choice feeding system the use of whole corn grain is recommended.

Kinetic aspects of rat (Rattus-norvegicus albinus) submandibular glands lactic-dehydrogenase

Submandibular glands of male rats were homogenized with 33 mM sodium potassium phosphate buffer, pH 6.5, containing 1 mM MgCl2 and 0.1 mM DTT and purified with ammonium sulphate, phosphocellulose chromatography, eluted with KC1 0.5 M, followed by Blue Sepharose CL-6B chromatography, eluted with NADH 0.5 mM. The enzyme kepts stable for 60 days when stored at -15-degrees-C in 33 mM phosphate buffer. In other experiment the enzyme was purified by oxamate-agarose chromatography from a crude extract of submandibular gland and the results obtained were better than by phosphocellulose and Sepharose CL-6B chromatography. The Km values for pyruvate. NADH, lactate and NAD+ were established. Sodium oxamate at 0.1 and 0.9 mM concentrations inhibited the LDH activity by 40 and 85%, respectively (competitive); with sodium oxalate the inhibition was of 30% (uncompetitive) and with 3-acetyl pyridine adenine dinucleotide was 80%.

Electron transfer between adjacent layers in self-assembled films

A simple model of electron transfer is adapted to explain fluorescence quenching in self-assembled films of poly( p-phenylene vinylene) (PPV) alternating with poly(thiophene acetic acid) (PTAA). Quenching is caused by a photo-induced electron transfer between the excited PPV (donor, D) and the PTAA (acceptor, A). The electron-transfer process can be mediated by insertion of electronically inert spacing bilayers between the D and A layers, As the number of bilayers is increased, the fluorescence is gradually recovered which is explained theoretically by assuming that the electron-transfer rate can be described as k = Z exp(- beta r) where r is the distance between D and A. (C) 2000 Elsevier B.V. B.V. All rights reserved.

Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and-4, in bovine antral follicles

Paracrine cell signaling is believed to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family has been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR3c and FGFR4) are expressed in bovine antral follicles. RT-PCR was used to analyze bovine Fgf8, Fgfr3c and Fgfr4 mRNA levels in oocytes, and granulosa and theca cells. Fgf8 expression was detected in oocytes and in granulosa and theca cells; this expression pattern differs from that reported in rodents. Granulosa and theca cells, but not oocytes, expressed Fgfr3c, and expression in granulosa cells increased significantly with follicle estradiol content, a major indicator of follicle health. Fgfr4 expression was restricted to theca cells in the follicle, and decreased significantly with increasing follicle size. To investigate the potential regulation of Fgfr3c expression in the bovine granulosa, cells were cultured in serum-free medium with FSH or IGF-I; gene expression was upregulated by FSH but not by IGF-I. The FSH-responsive and developmentally regulated patterns of Fgfr3c mRNA expression suggest that this receptor is a potential mediator of paracrine signaling to granulosa cells during antral follicle growth in cattle.

ULTRASTRUCTURE OF THE HINNY (EQUUS-ASINUS X EQUUS-CABALLUS) SEMINIFEROUS EPITHELIUM

The gonads and the germinative cells of 3 male hinnies were studied with light and transmission electron microscopy with the aim to observe the development of germ cells and verify the morphological modifications due to the hybridization. The hinny seminiferous epithelium presented Sertoli cells and spermatogonia with normal features and anomalous spermatocytes I. The other cells from the spermatogenic sequence were not seen. Most of the alterations began to occur in the cytes I, which presented nuclear vacuolization and deposits of amorphous material between the carioteca and the nuclear lamina, forming vesicles, or exaggerated chromatin condensation, resulting in pyknosis. In the cytoplasm vacuolization was also observed, besides organelle destruction.The arrest of meiosis due to lock of chromosome homologies leads to germinative cell degeneration and, therefore, the spermatogenesis arrest. This fact causes a profound alteration in the seminiferous epithelium morphology in comparison with the parental species.

Analyses of fatigue data in 4340 high strength steel after cadmium electroplating

Bending fatigue tests were carried out to clarify the effects of heat treatment parameters: temperature and time after cadmium electroplating on a high strength steel, to avoid hydrogen embrittlement. Temperatures heat of 190 degrees C, 230 degrees C, 250 degrees C and 300 degrees C at 3, 8 and 24 hours together with the base material electroplated, with and without heat treatment, resulted in 14 conditions studied with respect to fatigue behaviour. Statistical data analysis was performed to identify the best combination temperature/time regarding fatigue strength of the ABNT 4340 steel and the results obtained revealed that the fatigue strength depend on temperature/time conditions.

Electrochemical study of hydroxychloroquine and its determination in plaquenil by differential pulse voltammetry

Hydroxychloroquine (HCQ) is a halogenated aminoquinoline that presents wide biological activity, often being used as an antimalarial drug. The electrochemical reduction of HCQ was investigated by cyclic voltammetry and chronoamperometry using glassy carbon electrodes. By cyclic voltammetry, in acid medium, only the cathodic peak was observed. The electrochemical behavior of this peak is dependent on pH and the electrodic process occurs through an ErCi mechanism. The electron number (le) consumed in the reduction of HCQ was obtained by chronoamperometry. A method for the electrochemical determination of HCQ in pharmaceutical tablets was developed using differential pulse voltammetry. The detection limit reached was 11.2 mug ml(-1) of HCQ with a relative standard deviation of 0.46%. A spectrophotometric study of HCQ has been also carried out utilizing a band at 343 nm. The obtained detection limit and the relative standard deviation were 0.1 mug ml(-1) and 0.36%, respectively. The electrochemical methods are sufficiently accurate and precise to be applied for HCQ determination, in laboratorial routine, which can be used to determine the drug at low level. (C) 2003 Elsevier B.V. B.V. All rights reserved.