Synthesis of isotope-labelled methoxypyrazine compounds as internal standards and quantitative determination of aroma methoxypyrazines in water and wines by solid-phase extraction with isotope dilution-GC-MS /

An efficient way of synthesizing the deuterium labelled analogues of three methoxypyrazine compounds: 2-d3-methoxy-3-isopropylpyrazine, 2-d3-methoxy-3- isobutylpyrazine, and 2-d3-methoxy-3-secbutylpyrazine, has been developed. To confirm that the deuterium labels had been incorporated into the expected positions in the molecules synthesized, the relevant characterization by NMR, HRMS and GC/MS analysis was conducted. Another part of this work involved quantitative determination of methoxypyrazines in water and wines. Solid-phase extraction (SPE) proved to be a suitable means for the sample separation and concentration prior to GC/MS analysis.Such factors as the presence of ethanol, salt, and acid have been investigated which can influence the recovery by SPE for the pyrazines from the water matrix. Significantly, in this work comparatively simple fractional distillation was attempted to replace the conventional steam distillation for pre-concentrating a sample with a relatively large volume prior to SPE. Finally, a real wine sample spiked with the relevant isotope-labelled methoxypyrazines was quantitatively analyzed, revealing that the wine with 10 beetles per litre contained 138 ppt of 2-methoxy-3-isopropylpyrazine. Interestingly, we have also found that 2-methoxy-3-secbutylpyrazine exhibits an extremely low detection limit in GC/MS analysis compared with the detection limit of the other two methoxypyrazines: 2- methoxy-3-isopropylpyrazine and 2-methoxy-3-isobutylpyrazine.

Co-operative education and the development of self-esteem, an internal locus of control and work habits

This study compared approximately 50 grade 12 students studying In th~ co-operative education mode with approximately 50 grade 12 students studying in a traditional English course. Measures of self-esteem, locus of control and work habits were compared before and at the conclusion of one semester's involvement in the different programs. Using Coopersmith's Self-Esteem Inventory, the students who had chosen to study in the co-operative education mode scored significantly higher than the students in the traditional course. At the end of the semester, the co-operative education students' scores remained significantly higher than the English students'. Although the test showed no sjgnifi~ant changes in self-esteem. anecdotal reports indicated that co-operative education students had increased self-esteem over the semester. No significant differences in locus of control were observed between the two groups at any time. Significant differences in work habits were observed. While both groups had the same number of absences and the same marks before taking these courses, students who were involved in co-operative education had significantly fewer absences and significantly higher marks than the students studying in the traditional course. Anecdotal reports also indicated an improv~ment in work habits for students who had been involved in co-operative education. Recommendations of the study are for further research to determine more exactly how self-esteem and work habits develop in co-operative education students. Also. students. parents, teachers. and administrators need to be made aware of the success of this program.

Memo regarding the drainage of marsh lands

Memo regarding the drainage of marsh lands (1 page, handwritten), Jan. 15, 1855.

Memorandum respecting the marsh drainage

Memorandum respecting the marsh drainage (2 ½ pages, handwritten), July 7, 1855.

Report by Jacob Misner on setting contracts for deepening and clearing ditches and estimates of quantities and costs of marsh drainage

Report by Jacob Misner on setting contracts for deepening and clearing ditches and estimates of quantities and costs of marsh drainage (3 ½ pages, handwritten). This is marked as a copy, July 14, 1855.

Estimate of amount of excavation done by A. Cook on the ditch for marsh lands drainage

Estimate of amount of excavation done by A. Cook on the ditch for marsh lands drainage, Aug. 29, 1855.

Estimate of work done on marsh drainage by A. Cook, signed by S.D. Woodruff

Estimate of work done on marsh drainage by A. Cook, signed by S.D. Woodruff, Aug. 31, 1855.

Estimate of work done on marsh lands drainage by Alexander Cook, signed by S.D. Woodruff

Estimate of work done on marsh lands drainage by Alexander Cook, signed by S.D. Woodruff, Nov. 10, 1855.

Estimate of work done on marsh lands drainage by A. Cook and A. Mains and an estimate of time on the marsh lands survey from September to November

Estimate of work done on marsh lands drainage by A. Cook and A. Mains and an estimate of time on the marsh lands survey from September to November by Fred Holmes, Thomas Baird and Joseph Simpson. This is signed by S.D. Woodruff, Nov. 29, 1855.

Estimate of work done on marsh lands drainage by Alexander Cook and Andrew Mains

Estimate of work done on marsh lands drainage by Alexander Cook and Andrew Mains. This is signed by S.D. Woodruff. Also on this sheet is pay roll for engineer services for the months of September, October and November, 1855. This was paid to Frederick Holmes, Thomas Baird, Joseph Simpson and Len [illegible name], Nov.30, 1855.

Estimates for marsh drainage sent to Dexter Deverardo from S.D. Woodruff for Alexander Cook and Andrew Mains

Estimates for marsh drainage sent to Dexter Deverardo from S.D. Woodruff for Alexander Cook and Andrew Mains, Nov. 30, 1855.