Effectiveness of AFLPs and retrotransposon-based markers for the identification of portuguese grapevine cultivars and clones

Grapevine germplasm, including 38 of the main Portuguese cultivars and three foreign cultivars, Pinot Noir, Pinot Blanc and Chasselas, used as a reference, and 37 true-to-type clones from the Alvarinho, Arinto, Loureiro, Moscatel Galego Branco, Trajadura and Vinhão cultivars were studied using AFLP and three retrotransposon-based molecular techniques, IRAP, REMAP and SSAP. To study the retrotransposon-based polymorphisms, 18 primers based on the LTR sequences of Tvv1, Gret1 and Vine-1 were used. In the analysis of 41 cultivars, 517 IRAP, REMAP, AFLP and SSAP fragments were obtained, 83% of which were polymorphic. For IRAP, only the Tvv1Fa primer amplified DNA fragments. In the REMAP analysis, the Tvv1Fa-Ms14 primer combination only produced polymorphic bands, and the Vine-1 primers produced mainly ISSR fragments. The highest number of polymorphic fragments was found for AFLP. Both AFLP and SSAP showed a greater capacity for identifying clones, resulting in 15 and 9 clones identified, respectively. Together, all of the techniques allowed for the identification of 54% of the studied clones, which is an important step in solving one of the challenges that viticulture currently faces.

Portuguese Vaulting System at the Dawn and Early Modern Period. Between tradition and Innovation

Portuguese Vaulting System at the Dawn and Early Modern Period. Between tradition and Innovation. III International Congress on Construction History . Brandenburg university of technology Cottbus, Germany. Colab: Soraya Genin, Krista de Jonge.

Recommendations for Creating and Implementing Climate Change Mitigation Strategies for Municipalities

The effects of climate change are beginning to show themselves globally and not enough is being done to counteract these changes. Institutional action is a necessity, and municipalities have an opportunity to fill this void by developing mitigation strategies that will reduce greenhouse gas emissions. This report critically reviews the municipal climate action plans for Portland, Oregon; Boulder, Colorado; and Toronto, Ontario and provides recommendations for other municipalities who wish to create and implement climate change mitigation plans.