Mitochondrial carbonic anhydrase CA VB: Differences in tissue distribution and pattern of evolution from those of CA VA suggest distinct physiological roles

A cDNA for a second mouse mitochondrial carbonic anhydrase (CA) called CA VB was identified by homology to the previously characterized murine CA V, now called CA VA. The full-length cDNA encodes a 317-aa precursor that contains a 33-aa classical mitochondrial leader sequence. Comparison of products expressed from cDNAs for murine CA VB and CA VA in COS cells revealed that both expressed active CAs that localized in mitochondria, and showed comparable activities in crude extracts and in mitochondria isolated from transfected COS cells. Northern blot analyses of total RNAs from mouse tissues and Western blot analyses of mouse tissue homogenates showed differences in tissue-specific expression between CA VB and CA VA. CA VB was readily detected in most tissues, while CA VA expression was limited to liver, skeletal muscle, and kidney. The human orthologue of murine CA VB was recently reported also. Comparison of the CA domain sequence of human CA VB with that reported here shows that the CA domains of CA VB are much more highly conserved between mouse and human (95% identity) than the CA domains of mouse and human CA VAs (78% identity). Analysis of phylogenetic relationships between these and other available human and mouse CA isozyme sequences revealed that mammalian CA VB evolved much more slowly than CA VA, accepting amino acid substitutions at least 4.5 times more slowly since each evolved from its respective human–mouse ancestral gene around 90 million years ago. Both the differences in tissue distribution and the much greater evolutionary constraints on CA VB sequences suggest that CA VB and CA VA have evolved to assume different physiological roles.

Oratio salutatoria et oratio valedictoria, ca. 1763

Undated and unattributed handwritten Latin salutatory and valedictory orations composed for the Harvard College Commencement. A modern note with the materials suggests Nathaniel Sparhawk (Harvard AB 1765) as the author, but the author was more likely Joseph Hooper (Harvard AB 1763), who delivered the orations for the 1763 Harvard Commencement. While the documents are undated, textual clues include mention of the command of George III in recent war against France and Spain, suggesting the speech was written soon after the Treaty of Paris which was signed in February 1763 to end the Seven Years' War. The speech also celebrates Harvard Tutor William Kneeland, who resigned from his position in July 1763, and mentions the illness of Professor Edward Wigglesworth (who died before the 1765 Commencement). The text also mentions Professor John Winthrop and Massachusetts Governor Francis Bernard.

Manuscript of a Divan of Hafiz : manuscript, ca. 1527

Artist unknown.

Catharine of Russia ; or, The child of the storm : an historical drama, founded on an incident in the life of Peter the Great.

Separated from some unknown collection.

La Controverse de 1270 à l'Université de Paris et son retentissement sur la pensée de s. Thomas d'Aquin ..

On cover: L'homme en discussion à l'Université de Paris en 1270

Fulham palace, formerly called Fulham house, and Fulham manor : a short account of the old manor house of Fulham, written at the wish of the bishop on the occasion of His Lordship's visit to America and Canada, 1907 /

"The bishop of London and the mission field": p. 43-91.

A memoir of the life of Peter the Great.

Mode of access: Internet.

The antient Syriac version of the Epistles of St. Ignatius to St. Polycarp, the Ephesians, and the Romans : together with extracts from his Epistles, collected from the writings of Severus of Antioch, Timotheus of Alexandria, and others : edited with an English translation and notes : also the Greek text of these three Epistles, corrected according to the authority of the Syriac version /

Text in Syriac, Greek and English.

Genealogical record of some of the Noyes descendants of James Nicholas and Peter Noyes

v. 1. Descendants of Nicholas Noyes.--v. 2. Descendants of James and Peter Noyes.

Le roman de Brut /

Mode of access: Internet.

Le roman de Brut,

Mode of access: Internet.

Hymns for occasional use in the Parish Church of St. Peter in Nottingham.

Without music.