Genetic and morphological characterisation of a new species of the genus Hysterothylacium (Nematoda) from Paralichthys isosceles Jordan, 1890 (Pisces: Teleostei) of the Neotropical Region, state of Rio de Janeiro, Brazil

Taking into account the difficulties of taxonomic identification of larval anisakid nematodes based on morphological characters, genetic analyses were performed, together with those usually applied, in order to identify anisakid larvae found in the flounder Paralichthys isosceles from the littoral of the state of Rio de Janeiro, Brazil. The analysis of 1,820 larvae revealed a new species, similar to Hysterothylacium MD, Hysterothylacium 2, Hysterothylacium KB and Hysterothylacium sp regarding the absence of the larval tooth, an excretory pore situated below the nerve ring level, and slender lateral alae. Moreover, the new species differs from Hysterothylacium fortalezae and Hysterothylacium reliquens with regard to the number and size of spines present on the tail end and from Hysterothylacium patagonicus by the absence of interlabia. The maximum parsimony and neighbour joining tree topologies based on the 18S ribosomal DNA gene, complete internal transcribed spacer region and cytochrome oxidase 2 (COII) gene demonstrated that the Brazilian larvae belong to Raphidascarididae and represent a unique genetic entity, confirmed as a new Hysterothylacium species. Furthermore, the new species presents COII genetic signatures and shares polymorphisms with Raphidascarididae members. This is the first description of a new anisakid species from Brazil through the integration of morphological and molecular taxonomy data.

Two new species and new records of biting midges of the genus Culicoides from northwestern Argentina (Diptera: Ceratopogonidae)

The following two new species of Culicoides from the Argentinean Yungas are described, illustrated and placed to subgenus or species group and compared with related congeners: Culicoides calchaqui Spinelli & Veggiani Aybar and Culicoides willinki Spinelli & Veggiani Aybar. Culicoides daedaloides Wirth & Blanton is recorded for the first time for Argentina and Culicoides pseudoheliconiae Felippe-Bauer is firstly mentioned from the northwestern region of the country.

New species of the South American loxandrine genus Metoncidus Bates (Coleoptera, Carabidae)

Two species are added to the previously monotypic genus Metoncidus Bates, 1871 (Carabidae, Loxandrini): M. epiphytus sp. nov. (type locality Peru: Loreto, Cocha Shinguito) and M. gracilus sp. nov. (type locality Peru: Tambopata, Madre de Dios). Information allowing identification of the genus from among other South American carabid genera and a key to adult individuals of Metoncidus species are provided.

Descriptions of new species with a key to identification of the genus Neodexiopsis Malloch (Diptera, Muscidae) in Brazil

Nine new species of Neodexiopsis Malloch from Paraná, southern Brazil, are described: Neodexiopsis cinerea sp. nov. and N. paranaensis sp. nov. from Ponta Grossa; N. facilis sp. nov., N. legitima sp. nov., N. similis sp. nov. and N. uber sp. nov. from Guarapuava; N. erecta sp. nov., N. pura sp. nov. and N. rara sp. nov. from Colombo. A key to the identification of the Brazilian species of Neodexiopsis is also presented.

Revision of the New World bee fly genus Heterostylum Macquart (Diptera, Bombyliidae, Bombyliinae)

The genus Heterostylum Macquart and five Neotropical species (H. ferrugineum (Fabricius, 1805), H. hirsutum (Thunberg, 1827), H. rufum (Olivier, 1789), H. haemorrhoicum (Loew, 1863) and H. pallipes Bigot, 1892) are redescribed. The other species, recently redescribed or described are only diagnosed, except for H. deani Painter, 1930, whose spermathecae are described and illustrated for the first time. The main characters of the external morphology were photographed and the male genitalia and female spermathecae illustrated. An identification key to all included species is also presented.

Systematics and distribution of the genus Johnsonius Marsh (Hymenoptera, Braconidae, Doryctinae) with description of two new species from Brazil

The genus Johnsonius Marsh includes five species, most of them known from Costa Rica and one known from Venezuela, Peru and Costa Rica. Two of these species and two new species were recently collected in Atlantic rain forest. Description of the new species, illustrations of some morphological characters, a key for identification of all known species and the new geographic records are presented.

A new species of the fungus-farming ant genus Mycetagroicus Brandão & Mayhé-Nunes (Hymenoptera, Formicidae, Attini)

The fungus-farming ant genus Mycetagroicus Brandão & Mayhé-Nunes was proposed based on three species from the Brazilian "Cerrado": M. cerradensis, M. triangularis and M. urbanus. Here we describe a new species of Attini ant of the genus Mycetagroicus, M. inflatus n. sp., based on two workers collected in eastern Pará State, Brazil. A new key for species identification, comments on differences among species and new geographical distribution data are furnished.

First record of the genus Myllaena Erichson from Brazil, description of a new species and annotated catalog of Myllaena species from the Neotropical region (Coleoptera, Staphylinidae, Aleocharinae)

The genus Myllaena Erichson, 1837, of the tribe Myllaenini Ganglbauer, 1895, is recorded from Brazil for the first time. A new species, Myllaena brasiliensis sp. nov., is described and illustrated; it is closely related to the insomnis species group, described from the Nearctic region. A short diagnosis of the genus and an annotated catalog of Myllaena species from the Neotropical region are also provided.

A new species of the genus Pimelerodius Vanin from the Amazon Region, with notes on the geografic distribution of Pimelerodius motacilla (Boheman) (Coleoptera, Curculionidae, Otidocephalini)

Pimelerodius punctiventris sp. nov. (type locality Brazil, Amazonas, Itacoatiara) is described and illustrated. The new taxon is compared with similar species, being distinguished from the other 12 known species of the genus by the presence of punctures in ventrite I. The available published key for identification of species of Pimelerodius is adapted to include the new species. A modification of the generic description of the aedeagus of Pimelerodius is provided, a necessity due to the differences observed in the aedeagus of the new species. The occurrence of P. motacilla (Boheman, 1843) in the Amazon Region, recorded in sympatry with P. punctiventris in Itacoatiara, AM, is discussed and confirmed, based on the study of 41 available specimens.

A new species of Solepyris Azevedo (Hymenoptera, Bethylidae) from Brazil, with amended diagnosis of the genus

Solepyris Azevedo is a rarely collected Neotropical genus. It is differentiated from other Sclerodermini genera by having the fore wing venation with a single large closed cell and currently is monotypic containing S. unicus Azevedo. A second species, Solepyris montuosus, sp. nov., from Brazil is described and illustrated. This new species is diagnosed by having radial vein of the fore wing long. An amended diagnosis of Solepyris unicus Azevedo is included. A key for the two species of Solepyris is provided.

Two new Neotropical species of the genus Oxysarcodexia Townsend (Diptera, Sarcophagidae)

Two new Neotropical species of the genus Oxysarcodexia Townsend (Diptera, Sarcophagidae). Oxysarcodexia nitida sp. nov. and O. notata sp. nov. from Peru are described and illustrated based on male specimens. These new taxa are morphologically more similar to O. vittata (Walker) and O. xon (Dodge) respectively.

A new species of Dichanthidium (Hymenoptera, Megachilinae) and first records of the genus in Brazil

The second species of Dichanthidium, D. veredicola sp. nov., is herein described and illustrated from specimens collected in Berizal, Minas Gerais, in southeastern Brazil. Dichanthidium exile Moure is reported for the first time in Brazil, from Corumbá, Mato Grosso do Sul. A diagnosis and a key for the species of the genus are provided.

A new name for the Neotropical genus Aniarella Enderlein (Diptera, Sciaridae)

A new name for the Neotropical genus Aniarella Enderlein (Diptera, Sciaridae). A junior homonym was detected among neotropical sciarid flies genera and the following replacement name is proposed: Novaniarella nom. nov. for Aniarella Enderlein, 1911 (nec Bolivar, 1906). Accordingly, new combinations are herein proposed for the species currently included in this genus: Novaniarella azteca (Lane, 1959) comb. nov., Novaniarella brevis (Rubsaamen, 1894) comb. nov. and Novaniarella pelluscens (Enderlein, 1911) comb. nov.

A new species of Aristobrotica Bechynéand a checklist of the genus (Coleoptera, Chrysomelidae, Galerucinae)

A new species of Aristobrotica Bechynéand a checklist of the genus (Coleoptera, Chrysomelidae, Galerucinae). A new neotropical species of the Section Diabroticites (Luperini, Diabroticina) is described and illustrated. Aristobrotica capillosa sp. nov., from the state of Amazonas, northern Brazil, is the only species of the genus with elytra densely covered by erect hairs. The genus Aristobrotica Bechyné, 1956 now comprises 17 species, with only one occurring in Central America, Aristobrotica allardi (Jacoby, 1887) from Panama, and the remaining 16 are South American. A checklist of Aristobrotica species and their geographic distribution is also provided.

Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae)

Brachylophora, a new brachypterous genus of Rhopalophorini (Coleoptera, Cerambycidae). Brachylophora auricollis (Bruch, 1918) comb. nov. = Pasiphyle auricollis Bruch, 1918, originally described from Argentina (Salta), is redescribed and illustrated. Although with reduced elytra, the genus is transferred from Rhinotragini to Rhopalophorini based on the following characters: eyes well separated in both sexes, frons between eyes depressed and lacking frontal suture; pro-, meso-, and metasternum planar; mesothorax parallel-sided, not at all declivous before mesosternal process; metasternum large, together with mesosternum twice length of prosternum, metepisternum very wide, entire suture separating it from metasternum clearly visible when viewed from below; female ovipositor shortened with short cylindrical styles; and, more generally, structural features of hind legs, and surface ornamentation. Habitus similar to Coremia group. Bolivian specimens were netted as they visited flowers of Croton sp. (Euphorbiaceae).