Peritoneal dialysis per se is a risk factor for sclerostin-associated adynamic bone disease

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Chromosomal characteristics and distribution of constitutive heterochromatin in the Matogrossensis and Rubrovaria subcomplexes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Immunofluorescence and ultrastructural analysis of the chromatoid body during spermatogenesis of Triatoma platensis and T. rubrovaria (Hemiptera, Triatominae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A kinetic study of the main guaco metabolites using syrup formulation and the identification of an alternative route of coumarin metabolism in humans

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Plant identification based on leaf midrib cross-section images using fractal descriptors

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Rastros em chão branco: o sertão de João Guimarães Rosa entre percepções e memórias de travessias

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Marcadores prosódicos da escrita literária: uma análise da obra Manuelzão e Miguilim, de Guimarães Rosa

to each other. These approaches reveal the speaker's attitudes, their feelings expressed in statements and in the contexts in which those expressions are used. Cagliari (1989) calls this kind of discursive reference as prosodic markers in literary writing. In this project, the corpus for the analysis comes from the work of Guimarães Rosa: Manuelzão e Miguilim. The main goal of the present project is to develop studies on the subject, since there is virtually nothing done about the prosodic writing markers. Develop working methods is an important objective, in order to show how the prosodic markers can be studied. This type of study is not only important to linguistics: phonetics, textual analysis and discourse, but also to literary studies. Different ages of literary expressions used differently such resources as shown by the work of Cagliari (1989). The methodology of this project starts collecting data to compose the corpus with examples categorized as prosodic markers. Then, according to prosodic theories, these expressions are classified in types. The contexts in which they appear are important elements and they will be highlighted. The narrative of the plot is also an important context. Dialogues are good source of prosodic markers. In the work of Guimarães Rosa: Manuelzão e Miguilim, it was observed that the author likes to reveal the feelings of the characters in the novel through stated words in their speech. There are prosodic markers showing feeling like: intimidating, serene, gentle, cheerful, worried, angry, with irony, etc. The authors also refer to the fact that the character pronounced his speech with different voice qualities such as loud, hoarse, whispering, etc. This project studies the association between some prosodic elements of speech and their occurrence in literary texts as prosodic writing markers, as defined in the project. The data come from the phonetic descriptions of words and expressions regarded as prosodic...

Marcadores prosódicos da escrita literária: uma análise da obra Manuelzão e Miguilim, de Guimarães Rosa

to each other. These approaches reveal the speaker's attitudes, their feelings expressed in statements and in the contexts in which those expressions are used. Cagliari (1989) calls this kind of discursive reference as prosodic markers in literary writing. In this project, the corpus for the analysis comes from the work of Guimarães Rosa: Manuelzão e Miguilim. The main goal of the present project is to develop studies on the subject, since there is virtually nothing done about the prosodic writing markers. Develop working methods is an important objective, in order to show how the prosodic markers can be studied. This type of study is not only important to linguistics: phonetics, textual analysis and discourse, but also to literary studies. Different ages of literary expressions used differently such resources as shown by the work of Cagliari (1989). The methodology of this project starts collecting data to compose the corpus with examples categorized as prosodic markers. Then, according to prosodic theories, these expressions are classified in types. The contexts in which they appear are important elements and they will be highlighted. The narrative of the plot is also an important context. Dialogues are good source of prosodic markers. In the work of Guimarães Rosa: Manuelzão e Miguilim, it was observed that the author likes to reveal the feelings of the characters in the novel through stated words in their speech. There are prosodic markers showing feeling like: intimidating, serene, gentle, cheerful, worried, angry, with irony, etc. The authors also refer to the fact that the character pronounced his speech with different voice qualities such as loud, hoarse, whispering, etc. This project studies the association between some prosodic elements of speech and their occurrence in literary texts as prosodic writing markers, as defined in the project. The data come from the phonetic descriptions of words and expressions regarded as prosodic...

Acetylcysteine for Prevention of Renal Outcomes in Patients Undergoing Coronary and Peripheral Vascular Angiography Main Results From the Randomized Acetylcysteine for Contrast-Induced Nephropathy Trial (ACT)

Background-It remains uncertain whether acetylcysteine prevents contrast-induced acute kidney injury. Methods and Results-We randomly assigned 2308 patients undergoing an intravascular angiographic procedure with at least 1 risk factor for contrast-induced acute kidney injury (age >70 years, renal failure, diabetes mellitus, heart failure, or hypotension) to acetylcysteine 1200 mg or placebo. The study drugs were administered orally twice daily for 2 doses before and 2 doses after the procedure. The allocation was concealed (central Web-based randomization). All analysis followed the intention-to-treat principle. The incidence of contrast-induced acute kidney injury (primary end point) was 12.7% in the acetylcysteine group and 12.7% in the control group (relative risk, 1.00; 95% confidence interval, 0.81 to 1.25; P = 0.97). A combined end point of mortality or need for dialysis at 30 days was also similar in both groups (2.2% and 2.3%, respectively; hazard ratio, 0.97; 95% confidence interval, 0.56 to 1.69; P = 0.92). Consistent effects were observed in all subgroups analyzed, including those with renal impairment. Conclusions-In this large randomized trial, we found that acetylcysteine does not reduce the risk of contrast-induced acute kidney injury or other clinically relevant outcomes in at-risk patients undergoing coronary and peripheral vascular angiography.

Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agroindustrial residues

The filamentous fungus Paecylomices variotii was able to produce high levels of cell extract and extracellular invertases when grown under submerged fermentation (SbmF) and solid-state fermentation, using agroindustrial products or residues as substrates, mainly soy bran and wheat bran, at 40A degrees C for 72 h and 96 h, respectively. Addition of glucose or fructose (a parts per thousand yen1%; w/v) in SbmF inhibited enzyme production, while the addition of 1% (w/v) peptone as organic nitrogen source enhanced the production by 3.7-fold. However, 1% (w/v) (NH4)(2)HPO4 inhibited enzyme production around 80%. The extracellular form was purified until electrophoretic homogeneity (10.5-fold with 33% recovery) by DEAE-Fractogel and Sephacryl S-200 chromatography. The enzyme is a monomer with molecular mass of 102 kDa estimated by SDS-PAGE with carbohydrate content of 53.6%. Optima of temperature and pH for both, extracellular and cell extract invertases, were 60A degrees C and 4.0-4.5, respectively. Both invertases were stable for 1 h at 60A degrees C with half-lives of 10 min at 70A degrees C. Mg2+, Ba2+ and Mn2+ activated both extracellular and cell extract invertases from P. variotii. The kinetic parameters K-m and V-max for the purified extracellular enzyme corresponded to 2.5 mM and 481 U/mg prot(-1), respectively.

Eucalyptus growth promotion by endophytic Bacillus spp

Clonal eucalyptus plantings have increased in recent years; however, some clones with high production characteristics have vegetative propagation problems because of weak root and aerial development. Endophytic microorganisms live inside healthy plants without causing any damage to their hosts and can be beneficial, acting as plant growth promoters. We isolated endophytic bacteria from eucalyptus plants and evaluated their potential in plant growth promotion of clonal plantlets of Eucalyptus urophylla x E. grandis, known as the hybrid, E. urograndis. Eighteen isolates of E. urograndis, clone 4622, were tested for plant growth promotion using the same clone. These isolates were also evaluated for indole acetic acid production and their potential for nitrogen fixation and phosphate solubilization. The isolates were identified by partial sequencing of 16S rRNA. Bacillus subtilis was the most prevalent species. Several Bacillus species, including B. licheniformis and B. subtilis, were found for the first time as endophytes of eucalyptus. Bacillus sp strain EUCB 10 significantly increased the growth of the root and aerial parts of eucalyptus plantlets under greenhouse conditions, during the summer and winter seasons.

Amino acids in squamous cell carcinomas and adjacent normal tissues from patients with larynx and oral cavity lesions

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Hereditary Autoinflammatory Syndromes: A Brazilian Multicenter Study

To evaluate the prevalence of genetic defects in clinically suspected autoinflammatory syndromes (AIS) in a Brazilian multicenter study. The study included 102 patients with a clinical diagnosis of Cryopyrin Associated Periodic Syndromes (CAPS), TNF Receptor Associated Periodic Syndrome (TRAPS), Familial Mediterranean Fever (FMF), Mevalonate Kinase Deficiency (MKD) and Pediatric Granulomatous Arthritis (PGA). One of the five AIS-related genes (NLRP3, TNFRSF1A, MEFV, MVK and NOD2) was evaluated in each patient by direct DNA sequencing, based on the most probable clinical suspect. Clinical diagnoses of the 102 patients were: CAPS (n = 28), TRAPS (n = 31), FMF (n = 17), MKD (n = 17) and PGA (n = 9). Of them, 27/102 (26 %) had a confirmed genetic diagnosis: 6/28 (21 %) CAPS patients, 7/31 (23 %) TRAPS, 3/17 (18 %) FMF, 3/17 (18 %) MKD and 8/9 (89 %) PGA. We have found that approximately one third of the Brazilian patients with a clinical suspicion of AIS have a confirmed genetic diagnosis.