Comparative study of biphasic calcium phosphates with different HA/TCP ratios in mandibular bone defects. A long-term histomorphometric study in minipigs

Three biphasic calcium phosphate (BCP) bone substitute materials with hydroxyapatite (HA)/tricalcium phosphate (TCP) ratios of 20/80, 60/40, and 80/20 were compared to coagulum, particulated autogenous bone, and deproteinized bovine bone mineral (DBBM) in membrane-protected bone defects. The defects were prepared in the mandibles of 24 minipigs that were divided into four groups of six with healing times of 4, 13, 26, and 52 weeks, respectively. The histologic and histomorphometric evaluation focused on differences in amount and pattern of bone formation, filler degradation, and the interface between bone and filler. Collapse of the expanded polytetrafluoroethylene barrier membrane into the coagulum defects underlined the necessity of a filler material to maintain the augmented volume. Quantitatively, BCP 20/80 showed bone formation and degradation of the filler material similar to autografts, whereas BCP 60/40 and BCP 80/20 rather equaled DBBM. Among the three BCP's, the amount of bone formation and degradation of filler material seemed to be inversely proportional to the HA/TCP ratio. The fraction of filler surface covered with bone was highest for autografts at all time points and was higher for DBBM than BCP 80/20 and 60/40 at the early healing phase. TRAP-positive multinucleated cells were identified on BCP and DBBM surfaces without showing typical signs of resorption lacunae.

Molecular analysis of TNF sensitivity in normal and Ha-{\it ras\/} transformed murine fibroblasts

Tumor necrosis factor (TNF) is known to have antiproliferative effects on a wide variety of tumor cells but proliferative effects on normal cells. However, the molecular basis for such differences in the action of TNF are unknown. The overall objectives of my research are to investigate the role of oncogenes in TNF sensitivity and delineate some of the molecular mechanisms involved in TNF sensitivity and resistance. To accomplish these objectives, I transfected TNF-resistant C3H mouse embryo fibroblasts (10T1/2) with an activated Ha-ras oncogene and determined whether these cells exhibit altered sensitivity to TNF. The results indicated that 10T1/2 cells transfected with an activated Ha-ras oncogene (10T-EJ) not only produced tumors in nude mice but also exhibited extreme sensitivity to cytolysis by TNF. In contrast, 10T1/2 cells transfected with the pSV2-neo gene alone were resistant to the cytotoxic effects of TNF. I also found that TNF-induced cell death was mediated through apoptosis. The differential sensitivity of 10T1/2 and 10T-EJ cell lines to TNF was not due to differences in the number of TNF receptors on their cell surface. In addition, TNF-resistant revertants isolated from Ha-ras-transformed, TNF-sensitive cells still expressed the same amount of p21 as TNF-sensitive cells and were still tumorigenic, suggesting that Ha-ras-induced transformation and TNF sensitivity may follow different pathways. Interestingly, TNF-resistant but not sensitive cells expressed higher levels of bcl-2, c-myc, and manganese superoxide dismutase (MnSOD) mRNA following exposure to TNF. However, TNF treatment resulted in a marginal induction of p53 mRNA in both TNF-sensitive and resistant cells. Based on these results I can conclude that (i) Ha-ras oncogene induces both transformation and TNF sensitivity, (ii) TNF-induced cytotoxicity involves apoptosis, and (iii) TNF-induced upregulation of bcl-2, c-myc, and MnSOD genes is associated with TNF resistance in C3H mouse embryo fibroblasts. ^

Seder tehijat ha-metim : s. de resurrectione mortuorum principum rabbinorum sententia

Giessen, Phil., Diss., 1673

ha- ʿEt ṿe-ha-da : das Judenthum der Neuzeit ; Vorlesungen (1 - 5) geh. im Schulhause d. isr. Gemeinde zu Wieselburg

von H. L. Reich

Sefer ha-ḥinukh le-yalde bene Yiśraʾel

by H. A. Henry

Shorshe ha-dat=Lehrbuch der mosaischen Religion : nebst e. Anh., Ceremonialgesetze u. Gebräuche ... enthaltend

von J. Johlson

Jesode ha-thora : Glaubens- u. Pflichtenlehre für israelitische Schulen : mit e. kurzen Abriß d. bibl. u. nachbibl. Geschichte bis auf unsere Zeit

von S. Herxheimer

Dibre ha-berit, aseret ha-debarim : zehn Wort des Bundes oder die sogenannten zehn Gebote für Jung und Alt, besonders für den Religions-Unterricht in israelitischen Schulen

von Selig Louis Liepmannssohn

Jesode ha-thora : Israelitische Glaubens- und Pflichtenlehre für Schule und Haus

von S. Herxheimer

Religionsdisputationen im Mittelalter : populär-wissenschaftl. Vortr. gehalten im Bet ha Midrasch zu Wien

von Julius David

Midrash ha-zohar : die Religionsphilosophie des Sohar und ihr Verhältnis zur allgemeinen jüdischen Theologie : zugleich eine kritische Beleuchtung der Franck'schen "Kabbala"

von D. H. Joel

Midrash ha-zohar : die Religionsphilosophie des Sohar und ihr Verhältnis zur allgemeinen jüdischen Theologie : zugleich eine kritische Beleuchtung der Franck'schen "Kabbala"

von D. H. Joel