Association of midazolam with ketamine in giant Amazon river turtles Podocnemis expansa breed in captivity

OBJETIVO: Avaliar os efeitos de duas associações anestésicas em tartarugas da Amazônia em (Podocnemis expansa). MÉTODOS: Vinte P. expansa, hígidas, de ambos os sexos, com massa corporal entre 1,0 e 1,5 kg, de um criatório comercial localizado no vale do rio Araguaia, Goiás, Brasil, foram distribuídas em dois grupos (G1 n=10 e G2 n=10). Cada grupo recebeu um protocolo sendo: P1 = midazolam (2 mg/kg IM) com cetamina (20 mg/kg IM) e P2 = midazolam (2 mg/kg IM) com cetamina (60 mg/kg IM), aplicados nos grupos G1 e G2, respectivamente. Os fármacos foram aplicados no membro torácico esquerdo. Os parâmetros clínicos avaliados foram: locomoção, relaxamento muscular, resposta aos estímulos dolorosos nos membros torácico direito e pelvinos e freqüência cardíaca. Essas avaliações foram feitas no tempo 0 (imediatamente após a injeção) e nos tempos 5, 10, 20, 30, 45, 60, 90, 120, 150 e 180 minutos após as injeções. RESULTADOS: O G2 apresentou maior freqüência cardíaca que o G1 e imobilização mais rápida e prolongada. CONCLUSÃO: As sedações obtidas por esses protocolos (P1 e P2) foram satisfatórias, sendo possível a contenção farmacológica para a coleta de amostras biológicas e exame físico em P. expansa.

Description of an injury in a human caused by a false tocandira (Dinoponera gigantea, Perty, 1833) with a revision on folkloric, pharmacological and clinical aspects of the giant ants of the genera Paraponera and Dinoponera (sub-family Ponerinae)

A partir de um acidente causado pela picada de uma formiga falsa tocandira na mão de um pescador amador, os autores descrevem os achados clínicos locais observados, tais como edema, eritema e dor excruciante e a evolução do envenenamento, que cursou com fenômenos sistêmicos imediatos, como sudorese fria, náuseas, vômitos, mal estar, taquicardia e linfadenopatia axilar à esquerda. Após três horas, a dor intensa persistia e o paciente apresentou um episódio de hematoquesia, sem história anterior de enfermidades do trato digestivo, hematológicas ou vasculares. O uso de analgésicos (Tramal® 300 mg/dia), água quente e gelo não melhorou a dor, que arrefeceu em oito horas, tendo permanecido por cerca de 24 horas. São apresentados ainda os aspectos folclóricos, farmacológicos e clínicos relacionados às picadas de tocandiras.

SEMICLASSICAL ESTIMATE OF THE COULOMB-EXCITATION OF THE GIANT-DIPOLE RESONANCE IN PB-208

A semiclassical approach to study pure Coulomb excitation of Pb-208 giant dipole isovector resonance is examined. We consider medium energy projectiles and assume the target excitation to be described by a simple Goldhaber-Teller model. It is shown that the main features concerning the angular distribution are obtained in the angular range described by the model and an estimate is made of the pure Coulomb dipole contribution to the measured cross sections.

Ytterbium-induced energy-transfer upconversion enhancement in Nd3+/Pr3+-codoped PbGeO3-PbF2-CdF2 glass excited at 810nm

The effect of ytterbium ions upon energy transfer (ET) excited upconversion emission in Nd3+/Pr3+ -codoped PbGeO3-PbF2-CdF2 glass under 810 nm diode laser excitation is investigated. The results revealed that the presence of Yb3+ ions in the Nd3+/Pr3+-doped sample yields a fourfold enhancement in the visible and near infrared upconversion luminescence. The dependence of the upconversion process upon the excitation power, Nd3+, and Yb3+ concentrations is examined. The results indicated that ytterbium plays a major role in the ET upconversion process by bridging the 810nm neodymium excitation to praseodymium ions. The population of the Pr3+ ions P-3(0) emitting level was accomplished through a multi-ion interaction involving ground-state and excited-state absorption of pump photons at 810 nm by the Nd3+ followed by successive ET involving the Nd3+-Yb3+ and Yb3+-Pr3+ pairs. There is also direct ET Nd3+-Pr3+. (c) 2005 Elsevier B.V. All rights reserved.

ENHANCEMENT OF U-234 IN SPRINGWATERS OF AGUAS-DA-PRATA, SAO-PAULO, BRAZIL

Springwater samples were collected at Aguas da Prata, an important spa in São Paulo State, Brazil. The U-238 and U-234 isotopes were evaluated for Platina, Paiol, Villela, Prata-Radioativa, Prata-Nova, Boi, Vitoria, and Prata-Antiga springs. An inverse logarithmic correlation was found between U-234/U-238 activity ratio and Rn-222 content of the studied waters, which was related to different solution mechanisms of Rn-222 and U-234 nuclides at the rock-water interface. A linear correlation also exists between the U-234/U-238 activity ratios of the studied waters and total dissolved solids, ionic strength, CO2 partial pressure, and index of base exchange, which indicates that the enhancement of U-234 in solution increases with the degree of weathering in the aquifers, A higher U-234/U-238 activity ratio was found to be directly related to a higher value of dissolution rate.

BINDING OF PARACOCCIDIOIDES-BRASILIENSIS TO LAMININ THROUGH SURFACE GLYCOPROTEIN GP43 LEADS TO ENHANCEMENT OF FUNGAL PATHOGENESIS

Extracellular matrix protein laminin binds specifically to yeast forms of Paracoccidioides brasiliensis and enhances adhesion of the fungus to the surface of epithelial Madin-Darby canine kidney cells in vitro. Immunoblotting of fungal extracts showed that the gp43 glycoprotein is responsible for adhesion. This was confirmed by binding assays using purified gp43, with a K-d of 3.7 nM. The coating of P. brasiliensis yeast forms with laminin before injection into hamster testicles enhanced the fungus virulence, resulting in a faster and more severe granulomatous disease. These results indicate that interaction of fungi with extracellular matrix elements may constitute a basis for the evolution of fungal infection toward regional spreading and dissemination.

Twentyfold blue upconversion emission enhancement through thermal effects in Pr3+/Yb3+-codoped fluoroindate glasses excited at 1.064 mu m

Infrared-to-visible upconversion emission enhancement through thermal effects in Yb3+-sensitized Pr3+-doped fluoroindate glasses excited at 1.064 mu m is investigated. A twentyfold increase in the 485 nm blue emission intensity as the sample temperature was varied from 20 to 260 degrees C was observed. The visible upconversion fluorescence enhancement is ascribed to the temperature dependent multiphonon-assisted anti-Stokes excitation of the ytterbium sensitizer and excited-state absorption of the praseodymium acceptor. A model based upon conventional rate equations considering a temperature dependent effective absorption cross section for the F-2(7/2)-->F-2(5/2) transition of the Yb3+ and (1)G(4)-->P-3(0) excited-state absorption of the Pr3+, agrees very well with the experimental results. (C) 2000 American Institute of Physics. [S0021-8979(00)08209-8].

A possible stellar metallic enhancement in post-T Tauri stars by a planetesimal bombardment

The photospheres of stars hosting planets have larger metallicity than stars lacking planets. This could be the result of a metallic star contamination produced by the bombarding of hydrogen-deficient solid bodies. In the present work we study the possibility of an earlier metal enrichment of the photospheres by means of impacting planetesimals during the first 20-30 Myr. Here we explore this contamination process by simulating the interactions of an inward migrating planet with a disc of planetesimal interior to its orbit. The results show the percentage of planetesimals that fall on the star. We identified the dependence of the planet's eccentricity (e(p)) and time-scale of migration (tau) on the rate of infalling planetesimals. For very fast migrations (tau= 10(2) and 10(3) yr) there is no capture in mean motion resonances, independently of the value of e(p). Then, due to the planet's migration the planetesimals suffer close approaches with the planet and more than 80 per cent of them are ejected from the system. For slow migrations (tau= 10(5)and 10(6) yr) the percentage of collisions with the planet decreases with the increase of the planet's eccentricity. For e(p) = 0 and 0.1 most of the planetesimals were captured in the 2:1 resonance and more than 65 per cent of them collided with the star. Whereas migration of a Jupiter mass planet to very short pericentric distances requires unrealistic high disc masses, these requirements are much smaller for smaller migrating planets. Our simulations for a slowly migrating 0.1 M-Jupiter planet, even demanding a possible primitive disc three times more massive than a primitive solar nebula, produces maximum [Fe/H] enrichments of the order of 0.18 dex. These calculations open possibilities to explain hot Jupiter exoplanet metallicities.

Enhancement of natural killer cell function by titanocenes in mice bearing Ehrlich ascites tumour

In the present work, we studied the effects of two titanocenes, biscyclopentadienyidichlorotitanium IV, (DDCT) and its derivative, biscyclopentadienylditiocianatetitanium IV (BCDT), on the activity of natural killer (NK) cells in Ehrlich ascites tumour (EAT)-bearing BALB/c mice. In order to investigate a more direct effect of these compounds on NK cell function, we performed experiments with severe combined immunodeficiency (SCID) mice, which exhibit a normal NK cell response in the absence of T and B cells. The treatment consisted of intraperitoneal (i.p.) administration of 15 mg/kg/day of DDCT for 2 days or 10 mg/kg/day of BCDT for 3 days. In addition, to verify whether the effects produced by the titanocenes were compound specific or related to a direct antitumour effect, we also investigated the effects of a 3-day treatment with 100 mg/kg of cyclophosphamide cyclophosphamide on NK cell activity. Our results demonstrated that, in BALB/c and SCID mice, NK cell function declined to subnormal levels after inoculation of the tumour. In these animals, although treatment with DDCT and BCDT significantly enhanced NK cell function, only DDCT restored NK cell activity to normal values in all stages studied. Conversely, treatment with cyclophosphamide reduced NK cell function in nontumour bearing SCID mice and was also unable to restore the decreased NK activity of tumour-bearing SCID mice, thus demonstrating that the enhancement of NK cell function by titanocenes is compound specific. The same effect of cyclophosphamide was observed with BALB/c mice. In the present study, the up-modulatory effects of these two compounds on NK cell function reveal a new aspect of the mechanism of antitumoural action of titanocenes. (C) 2003 Elsevier B.V. All rights reserved.

Treatment of radius, ulna and humerus fractures with the aid of a bone morphogenetic protein in a giant anteater (Myrmecophaga tridactyla)

A giant anteater (Myrmecophaga tridactyla) was found with closed comminuted fractures on the fight radius and ulna and left humerus he duration of which was unknown. The animal was unable to use either of he thoracic limbs. The fractures were stabilized with 3.5-mm titanium plates and a commercially available mixture of micro lyophilized bovine cortical osseous and bovine BMP (Gen-tech(R), Baumer, Brazil) was implanted into the fractures sites. Postoperative radiographic evaluations were performed every 30 days and after four months. Bone healing was observed in all of he fractures. The animal was able to be reintroduced into its natural habitat. From his case we conclude that despite he low metabolic fate of the giant anteater, which is an inherent characteristic of this species, he treatment of radius, ulna and humerus fractures by means of plates and screws, associated with BMP on the Myrmecophaga tridactyla, was a success.

Determination of low-aliphatic aldehydes indoors by micellar electrokinetic chromatography using sample dissolution manipulation for signal enhancement

This work describes a novel approach for the analysis of selected aldehydes (formaldehyde, acetaldehyde, propionaldehyde, and acrolein) and acetone in environmental samples using micellar electrokinetic chromatography (MEKC). The method is based on the reaction of carbonyl compounds with 3-methyl-2-benzothiazoline hydrazone (MBTH) that gives an azine intermediate with maximum absorbance at 216 nm. A systematic evaluation of sample dissolution medium was conducted as a means to enhancing sensitivity. In the best condition, samples were dissolved in 0.030 mol.L-1 tetraborate solution. This condition presented enhancement factors in the range of 35-54 for the aldehydes under investigation, computed as the improvement of the concentration limits of detection (LODs) with reference to the sample dissolved in pure water. The running buffer was 0.020 mol.L-1 tetraborate, pH 9.3, containing 0.050 mol-L-1 sodium dodecyly sulfate (SIDS). The overall methodology presented several advantages over established methods for aldehydes. Worthy mentioning that MBTH is available in high purity degree, dispensing laborious reagent purification procedures. A few method validation parameters were determined revealing good migration time repeatability (< 2.5% coefficient of variation, CV) and area repeatability (< 4% CV), excellent linearity (20-120 mug/L, r > 0.995) and adequate sensitivity for environmental applications. The LODs with respect to each single aldehyde were in the range of 0.54-4.0 mug.L-1 and 11 mug.L-1 for acetone. The methodology was applied to the determination of aldehydes indoors. Samples were collected in an impinger flask containing 0.05% MBTH solution, at a flow rate of 0.80 L.min(-1), during 2.5 h, at different times during the day. The most abundant carbonyls in the samples were acetone, followed by formaldehyde and acetaldehyde, with estimate peak concentrations of 452, 5.2 and 2.2 ppbv, respectively.

Fungicidal activity of human monocyte-derived multinucleated giant cells induced in vitro by Paracoccidioides brasiliensis antigen

Multinucleated giant cells (MGC) are characteristic cells in granulomatous disorders such as paracoccidioidomycosis (PCM) and also are formed in vitro from peripheral blood mononuclear cells by several stimuli. In this study, the authors investigated in vitro formation of MGC derived from monocytes of healthy individuals, stimulated with Paracoccidioides brasiliensis antigen (PbAg), compared with other stimuli such as IFN-gamma and supernatant of Con-A-stimulated peripheral blood mononuclear cells (CM-ConA). Besides, the fungicidal activity of monocytes and monocyte-derived MGC challenged with P. brasiliensis were compared, at a ratio of one fungus per 50 monocytes. Results demonstrated that PbAg, IFN-gamma, and CM-ConA stimuli were able to induce MGC generation, with fusion indices significantly higher than control cultures. Striking results were observed when MGC induced by PbAg and IFN-gamma presented higher fungicidal activity than monocytes, submitted to the same stimuli, showing a better capacity of these cells to kill P. brasiliensis. In summary, the results suggest that PbAg is able to induce MGC generation, and these cells presented higher fungicidal activity against P. brasiliensis than monocytes.

Ventilation of the giant nests of Atta leaf-cutting ants: does underground circulating air enter the fungus chambers?

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)