884 resultados para Agentes corantes
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Pós-graduação em Medicina Veterinária - FCAV
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Pós-graduação em História - FCHS
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Pós-graduação em Psicologia - FCLAS
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Pós-graduação em Odontologia Restauradora - ICT
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Pós-graduação em Saúde Coletiva - FMB
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Odontologia Restauradora - ICT
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The aim of this in vitro study was to evaluate the trans-enamel and transdentinal cytotoxic effects of two in-office tooth bleaching techniques that employ bleaching gels containing 20% and 38% of H2 O2 on cultured odontoblast-like cell line (MDPC-23). Sixty enamel/dentin discs were obtained from bovine central incisors and placed individually in artificial pulp chambers. Six groups were formed according to the following enamel treatments: G1- 20% H2 O2 (1 application); G2- 20% H2 O2 (2 applications); G3- 38% H2 O2 (1 application); G4- 38% H2 O2 (2 applications); G5- 38% H2 O2 (3 applications); and G6- control (no treatment). In G1 and G2, the bleaching gel was left in contact with the enamel surface for 45 min in each application. However, in G3, G4, and G5 the bleaching gel was applied for only 10 min per application. After the last application, the extracts were collected and applied on previously cultured cells (30.000 cells/cm2 ) for 24 h. Cell metabolism was evaluated by the MTT assay and cell morphology was analysed by scanning electron microscopy. Cell metabolism decreased by 96.29%; 96.11%; 96.42%; 95.62%; and 97.18% in G1, G2, G3, G4, and G5, respectively. All treated groups differed significantly from non-treated control group (G6) (p < 0.05). However, the difference in cell metabolism among treated groups was not significant statistically. In addition, significant morphological cell alterations were observed in all treated groups. Under the tested experimental conditions, the extracts collected after both tooth bleaching techniques evaluated in this study caused severe toxic effects on cultured odontoblast-like cell MDPC-23.
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Dental tooth bleaching is a conservative option for the treatment of tooth stains. It is based on the use of hydrogen peroxide as an active agent. Despite its effectiveness to lighten tooth colour, there is concern regarding its use due to the effects it could have over enamel surface. There is scarce evidence on the subject and contradictions exist between different authors. The aim of this study was to compare enamel surface micromorphology after bleaching teeth with different concentrations of hydrogen peroxide solutions. Method: 50 healthy bovine incisors sectioned horizontally at the cemento-enamel junction were prepared. Contents of pulp chamber and tooth surfaces were cleaned. The buccal surface of each tooth was divided vertically, assigning one half to the control group (CG) and the other randomly to: Group 1: 25 samples treated with 15% hydrogen peroxide with nitrogen doped titanium dioxide. Group 2: 25 samples treated with 35% hydrogen peroxide. Square samples (2x2 mm.) were obtained and observed by SEM (magnification of 5.000x and 10.000x). Results: All treated groups showed longitudinal depressions on the surface and increased surface roughness. Conclusions: Tooth bleaching with hydrogen peroxide produces subclinical alterations over bovine enamel surface. 15% hydrogen peroxide bleaching agent produced less micromorphology alteration over bovine enamel surface than the 35% hydrogen peroxide agent.
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Pós-graduação em Medicina Veterinária - FCAV
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Pós-graduação em Educação - FCT
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Two experiments were carried out to evaluate the effect of adding glutamine, fish oil or yeast cellular wall to the diet of weaned piglets on the expression of the enzyme ornithine decarboxylase (ODC), the protein content and DNA in small intestine samples and on performance. In the first experiment, 24 weaned piglets were used to measure the performance at the phases prestarter, starter 1 and starter 2. Four diets were tested (T1 -basal diet (BD); T2 -BD + 1% of glutamine; T3 -BD + 0,2% of yeast cellular wall; T4 -BD + 5% of fish oil). At the second experiment 45 weaned piglets were used and distributed in a randomized block design, in factorial outline with four diets and three slaughter ages (on the day of weaning, on the seventh and fourteenth days postweaning). The tested diets did not alter the piglets' performance in none of the phases. There was reduction of the expression of the ODC enzyme, of the protein concentration and of the relationship protein/DNA to the seven days postweaning, with increase of the values on the 14th day, evidencing a state of hypotrophy of the mucous membrane, suggesting that the process of protein synthesis in the small intestine was diminished in the first week after weaning, but it presented recovery signs in the second week.
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Pós-graduação em Química - IBILCE