994 resultados para AIDE-GTP
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3e sér. Géologie et paléontologie des couches à Avicula contorta en Lombardie comprenant des aperçus sur l'étage infraliasien en Lombardie et en Europe en général et deux monographies des fossiles appartenant à la zone supérieure et à la zone inférieure des couches à Avicula contorta en Lombardie, une note supplementaire et deux appendices, Sur l'infralias du versant N-O des Alpes et Sur les faunes aux limites supérieures et inférieures des couches à A. contorta, par A. Stoppani. 1860-65.--4e sér. Monograhie des fossiles du calcaire rouge ammonitique (lias supérieur) de Lombardie et de l'Apennin central, par J. Meneghini; Fossiles du Medolo, appendice. 1867-81.
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Mode of access: Internet.
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Errata-t. 1: inserted.
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Mode of access: Internet.
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Mode of access: Internet.
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"Un résumé aide-mémoire des leçons de géographie économique, sur le Canada, faites à l'Ecole des hautes études commerciales de Montréal."
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Mode of access: Internet.
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Mode of access: Internet.
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The microlocalization of Ras proteins to different microdomains of the plasma membrane is critical for signaling specificity. Here we examine the complex membrane interactions of H-ras with a combination of FRAP on live cells to measure membrane affinity and electron microscopy of intact plasma membrane sheets to spatially map microdomains. We show that three separable forces operate on H-ras at the plasma membrane. The lipid anchor, comprising a processed CAAX motif and two palmitic acid residues, generates one attractive force that provides a high-affinity interaction with lipid rafts. The adjacent hypervariable linker domain provides a second attractive force but for nonraft plasma membrane microdomains. Operating against the attractive interaction of the lipid anchor for lipid rafts is a repulsive force generated by the N-terminal catalytic domain that increases when H-ras is GTP loaded. These observations lead directly to a novel mechanism that explains how H-ras lateral segregation is regulated by activation state: GTP loading decreases H-ras affinity for lipid rafts and allows the hypervariable linker domain to target to nonraft microdomains, the primary site of H-ras signaling.
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Septins (SEPTs) form a family of GTP-binding proteins implicated in cytoskeleton and membrane organization, cell division and host/pathogen interactions. The precise function of many family members remains elusive. We show that SEPT6 and SEPT7 complexes bound to F-actin regulate protein sorting during multivesicular body (MVB) biogenesis. These complexes bind AP-3, an adapter complex sorting cargos destined to remain in outer membranes of maturing endosomes, modulate AP-3 membrane interactions and the motility of AP-3-positive endosomes. These SEPT-AP interactions also influence the membrane interaction of ESCRT (endosomal-sorting complex required for transport)-I, which selects ubiquitinated cargos for degradation inside MVBs. Whereas our findings demonstrate that SEPT6 and SEPT7 function in the spatial, temporal organization of AP-3- and ESCRT-coated membrane domains, they uncover an unsuspected coordination of these sorting machineries during MVB biogenesis. This requires the E3 ubiquitin ligase LRSAM1, an AP-3 interactor regulating ESCRT-I sorting activity and whose mutations are linked with Charcot-Marie-Tooth neuropathies.
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Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.
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Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.
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Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.
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Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.
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Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.