O eterno retorno como paródia e como colagem na filosofia da diferença de Gilles Deleuze

Gilles Deleuze hás commented on many philosophers, but his relationship with Nietzsche plays a singular role in his thought: appropriating the concept of the “eternal return” to think the central axis of his thesis, Difference and repetition (1968). Terms “difference” and “repetition” appeared associated to eternal return in his Nietzsche and philosophy (1962). Our dissertation thesis analyzes the presentations of that concept in bothworks. Chapter one presents the style construction and critical, methodological aspects of Nietzschean philosophy, fundamental elements to understand Deleuze’s interpretation. It subsequently analyzes the first presentation of that concept, expressed in the following terms: the aesthetic existence, either innocent or justified from the figure of game. We will see how the image of game implies another concept of chance, that leads Deleuze to think of an affirmative philosophical “type”, capable of creating new values. Chapter two evaluates the existential, “ethical-selective”, “physicalcosmological” character of the concept of eternal return, as much as the difficulties it imposes upon Nietzsche’s interpreter. We present afterwards Deleuzian comprehension of eternal return as a “parody” or a “simulacrum of doctrine”. Chapter three analyzes that interpretive position as a transvaluation of values from a rearrange of perspectives in order to overcome the negative comprehensions of existence. We want to question the way Deleuze builds another image of thought from the concept of eternal return – an image that, by a sort of “colagem” and selective elimination of the negativity, proposes a historiographic work and unfolds a lineage of thinkers of immanence and difference, a detour from the thought of identity, the same and the similar. We want thus to understand Deleuze’s critique of “dogmatic image of thought”.

O eterno retorno como paródia e como colagem na filosofia da diferença de Gilles Deleuze

Gilles Deleuze hás commented on many philosophers, but his relationship with Nietzsche plays a singular role in his thought: appropriating the concept of the “eternal return” to think the central axis of his thesis, Difference and repetition (1968). Terms “difference” and “repetition” appeared associated to eternal return in his Nietzsche and philosophy (1962). Our dissertation thesis analyzes the presentations of that concept in bothworks. Chapter one presents the style construction and critical, methodological aspects of Nietzschean philosophy, fundamental elements to understand Deleuze’s interpretation. It subsequently analyzes the first presentation of that concept, expressed in the following terms: the aesthetic existence, either innocent or justified from the figure of game. We will see how the image of game implies another concept of chance, that leads Deleuze to think of an affirmative philosophical “type”, capable of creating new values. Chapter two evaluates the existential, “ethical-selective”, “physicalcosmological” character of the concept of eternal return, as much as the difficulties it imposes upon Nietzsche’s interpreter. We present afterwards Deleuzian comprehension of eternal return as a “parody” or a “simulacrum of doctrine”. Chapter three analyzes that interpretive position as a transvaluation of values from a rearrange of perspectives in order to overcome the negative comprehensions of existence. We want to question the way Deleuze builds another image of thought from the concept of eternal return – an image that, by a sort of “colagem” and selective elimination of the negativity, proposes a historiographic work and unfolds a lineage of thinkers of immanence and difference, a detour from the thought of identity, the same and the similar. We want thus to understand Deleuze’s critique of “dogmatic image of thought”.

Questionamentos acerca de valores axiológicos no turismo: uma abordagem fenomenológica hermenêutica

The study presents the possibility of interpretation of axiological values of tourism as a practice conceived on a human being or Dasein as being-in-the-world of tourism. The value, as an object of Axiology, was considered the predictor of the human being conduct in the phenomenon reflecting this same value in tourism. The aim was to comprehend and interpret through the way of being of Dasein in tourism, which axiological values are chosen to the practice of tourism and the intentional feelings directed to these values. A phenomenological hermeneutics research with exploratory characteristics was accomplished in order to survey the values. Ten episodic interviews were conducted from the hermeneutic situation - constituted by fore-having, fore-sight and fore-conception of each Dasein interviewed, by adopting a sympathetic conduct and sympathy of Max Scheler and the use of emotional intuition to capture the intentional feelings, interpreted afterwards by the analysis of a Martin Heidegger's phenomenology in Being and Time. The results showed that, even without categorisation, the totality of the living experiences, the way of being of positive values outnumber the negatives ones in the existence of each Dasein, leading them to the Learning, which are comprising: experiences to provide self-knowledge, historical-cultural values, and memory as part of the learning experiences, hospitality as a way of openness and socio-cultural exchange, solidarity and peace. Intentional feelings directed at the values for the choice for practicing tourism were: love, happiness, pleasure, respect and trust. Four evidences were found concerning the use of sentimental perspective and intentional feeling of Scheler and regarding the logic of the heart of Pascal used by this author. The sociocultural interrelationships and exchanges form the basis for developing tourism as phenomenon. Therefore the character being-with or Mitsein is prevalent in tourism activities. Despite the learning was the purpose of the experiences, the ultimate goal was the improvement and personal enrichment of Dasein´s humanity development. The study also showed the hermeneutic phenomenological seeing opens the access of the living experiences of values, without making arbitrarily judgment and achieve "to the thing themselves", which, by the overlapping of categories, dispositions and intentional feelings, form the evaluative experiences and are possible to access through the fundamental ontology of Heidegger. The study contributes to broaden the vision concerning to the totality of tourism and the practitioners Dasein of it. As possibilities for deepening studies, was pointed out: the total person of Scheler; the care or Sorge as a form of love in Heidegger; happiness and pleasure in the practice of tourism and human flourishing or eudaimonia.

Junho de 2013: o mês que não começou

This work aimed to analyze the so called June Journeys which happened in Brazil in 2013, in an effort to establish a proper ontology for cyberspace that goes beyond old dichotomies that put men and techniques in polar opposites, making difficult to perform a constructive – and not merely pessimist – analysis of the crossroads between politics and technology. This analysis uses as basis and methodological guidance the Theory of Actor-Network (ANT or TAR) widely used in social studies of science and technology to overcome these dichotomies and allow the analysis of networks, so that is clear the political instability that arises in this context which profoundly changes the political game.

Low frequency of cigarette smoking and the risk of head and neck cancer in the INHANCE consortium pooled analysis

Funding • The pooled data coordination team (PBoffetta, MH, YCAL) were supported by National Cancer Institute grant R03CA113157 and by National Institute of Dental and Craniofacial Research grant R03DE016611 • The Milan study (CLV) was supported by the Italian Association for Research on Cancer (Grant no. 10068). • The Aviano study (LDM) was supported by a grant from the Italian Association for Research on Cancer (AIRC), Italian League Against Cancer and Italian Ministry of Research • The Italy Multicenter study (DS) was supported by the Italian Association for Research on Cancer (AIRC), Italian League Against Cancer and Italian Ministry of Research. • The Study from Switzerland (FL) was supported by the Swiss League against Cancer and the Swiss Research against Cancer/Oncosuisse [KFS-700, OCS-1633]. • The central Europe study (PBoffetta, PBrenan, EF, JL, DM, PR, OS, NS-D) was supported by the World Cancer Research Fund and the European Commission INCOCOPERNICUS Program [Contract No. IC15- CT98-0332] • The New York multicentre study (JM) was supported by a grant from National Institute of Health [P01CA068384 K07CA104231]. • The study from the Fred Hutchison Cancer Research Center from Seattle (CC, SMS) was supported by a National Institute of Health grant [R01CA048996, R01DE012609]. • The Iowa study (ES) was supported by National Instituteof Health [NIDCR R01DE011979, NIDCR R01DE013110, FIRCA TW001500] and Veterans Affairs Merit Review Funds. • The North Carolina studies (AFO) were supported by National Institute of Health [R01CA061188], and in part by a grant from the National Institute of Environmental Health Sciences [P30ES010126]. • The Tampa study (PLazarus, JM) was supported by National Institute of Health grants [P01CA068384, K07CA104231, R01DE013158] • The Los Angeles study (Z-F Z, HM) was supported by grants from National Institute of Health [P50CA090388, R01DA011386, R03CA077954, T32CA009142, U01CA096134, R21ES011667] and the Alper Research Program for Environmental Genomics of the UCLA Jonsson Comprehensive Cancer Center. • The Houston study (EMS, GL) was supported by a grant from National Institute of Health [R01ES011740, R01CA100264]. • The Puerto Rico study (RBH, MPP) was supported by a grant from National Institutes of Health (NCI) US and NIDCR intramural programs. • The Latin America study (PBoffetta, PBrenan, MV, LF, MPC, AM, AWD, SK, VW-F) was supported by Fondo para la Investigacion Cientifica y Tecnologica (FONCYT) Argentina, IMIM (Barcelona), Fundaco de Amparo a‘ Pesquisa no Estado de Sao Paulo (FAPESP) [No 01/01768-2], and European Commission [IC18-CT97-0222] • The IARC multicentre study (SF, RH, XC) was supported by Fondo de Investigaciones Sanitarias (FIS) of the Spanish Government [FIS 97/ 0024, FIS 97/0662, BAE 01/5013], International Union Against Cancer (UICC), and Yamagiwa-Yoshida Memorial International Cancer Study Grant. • The Boston study (KKelsey, MMcC) was supported by a grant from National Institute of Health [R01CA078609, R01CA100679]. • The Rome study (SB, GC) was supported by AIRC (Italian Agency for Research on Cancer). • The US multicentre study (BW) was supported by The Intramural Program of the National Cancer Institute, National Institute of Health, United States. • The Sao Paolo study (V W-F) was supported by Fundacao de Ampara a Pesquisa no Estado de Sao Paulo (FAPESP No 10/51168-0) • The MSKCC study (SS, G-P Y) was supported by a grant from National Institute of Health [R01CA051845]. • The Seattle-Leo stud (FV) was supported by a grant from National Institute of Health [R01CA030022] • The western Europe Study (PBoffetta, IH, WA, PLagiou, DS, LS, FM, CH, KKjaerheim, DC, TMc, PT, AA, AZ) was supported by European Community (5th Frame work Programme) grant no QLK1-CT-2001- 00182. • The Germany Heidelberg study (HR) was supported by the grant No. 01GB9702/3 from the German Ministry of Education and Research.

Enfermagem: entre a hipertecnologia e um conhecimento estético do Cuidar

Os sistemas de saúde estão a enfrentar uma realidade de radical intensidade biotecnológica, em que a digitalização ocupa um lugar central na prestação de cuidados. A inovação tecnológica e a cyborguização do humano devem traduzir-se em novos conceitos do cuidar, em que a ontologia Cyborg tem um lugar central.

Tecnologia encarnada: entre a hipertecnologia e um conhecimento estético do Cuidar

Introdução: Os sistemas de saúde estão a enfrentar uma realidade de radical intensidade biotecnológica, em que a digitalização ocupa um lugar central na prestação de cuidados. A inovação tecnológica e a cyborguização do humano devem traduzir-se em novos conceitos do cuidar, em que a ontologia cyborg tem um lugar central. Objectivos: Através de um enquadramento conceptual e tendo em conta os referentes teóricos da enfermagem sobre o cuidar, pretende-se estruturar uma análise epistemológica sobre a tecnologia, a ontologia cyborg e novos conceitos de "Cuidar em Enfermagem Metodologia: Revisão integrada de literatura, análise reflexiva e conceptual de quadros teóricos referenciais em enfermagem. Resultados: A investigação recente neste domínio baseia-se num pressuposto essencialista que remete para uma determinada conceção de "Tecnologia" enquanto aparato instrumental redutor, numa abordagem epistemológica positivista e mecanicista da tecnociência. As tecnologias de ponta aplicadas à enfermagem (onde se incluem as tecnologias de informação, plataformas digitais informáticas, sistemas de e-health, tele-enfermagem) são consideradas nesta perspetiva pragmática, como algo neutro, preexistente, uma mera ferramenta ou utensílio facilitador ao dispor dos enfermeiros. A análise dos discursos científicos assim construídos revela a adoção de uma visão instrumental da tecnologia. Afirma-se a clivagem entre pessoas e máquinas, a absoluta distinção entre tecnologia e a condição humana, num hiato entre objetos "técnicos" e objectos "naturais". Conclusões: A clivagem dicotómica entre "Tecnologia" e "Cuidado centrado na Pessoa" é artificial, fragmentária e contraria uma conceção integradora do cuidar. A dimensão estética no cuidar em enfermagem pressupõe a plena assunção da ontologia Cyborg. A conceção de uma "tecnologia encarnada" permite integrar na relação do cuidado uma hermenêutica do descentramento, incorporando, nos fenómenos alvo da intervenção de enfermagem, as paisagens relacionais, no sentido de relationscapes de Erin Manning (2013), onde os processos tecnológicos de saúde e doença e os cuidados são efetivamente vividos.

Protemic characterization of peripheral blood mononuclear cells

Peripheral blood mononuclear cells (PBMCs) play quite diverse and important roles in monitoring immune homeostasis. Thus, these subset of blood cells may provide access to potential physiological relevant biomolecules, namely proteins. For this reason, PBMCs represent a promising biological sample in scientific research, particularly as a source of potential biological markers discovery of the most diverse diseases. Prior studies of proteomic characterization of PBMCs from healthy individuals lack either the identification of a large number of proteins or its quantification in a way that is compatible with the search of potential biomarker candidates. Therefore, this study aimed to provide a comprehensive PBMCs proteome characterisation as well as to create a SWATH library. It was also evaluated if by using the BD Vacutainer® CPT™ tubes for PBMCs isolation, it would be possible to identify a larger number of immunologically relevant proteins in comparison to plasma samples. The enrichment test assay revealed that it is possible to identify more immune-related proteins from isolated PBMCs than from plasma. Moreover, the majority of the quantified proteins with an “immune system” GO term assigned is present in higher amounts in PBMCs samples. 2D LC-MS/MS proved to be the best approach to use in qualitative analysis of PBMCs and in the construction of a SWATH library, since it resulted in an increase of both identified and quantified proteins (66.3% and 16.9%, respectively) in comparison to 1D LC-MS/MS. A total of 2071 proteins were identified and it was possible to quantify 922 different proteins among six distinct samples. From these proteins, 445 were commom between all individuals. In conclusion, this work provides a comprehensive PBMCs proteome dataset that will be useful in further studies that focus on the search for potential biological markers of various pathologies in these cells. Additionally, SWATH-MS proved to be a reproducible and effective acquisition method to quantify PBMCs proteins.

Enfermagem: Entre a hipertecnologia e um conhecimento estético

Os sistemas de saúde estão a enfrentar uma realidade de radical intensidade biotecnológica, em que a digitalização ocupa um lugar central na prestação de cuidados. A inovação tecnológica e a cyborguização do humano devem traduzir-se em novos conceitos do cuidar, em que a ontologia Cyborg tem um lugar central.

Tecnologia encarnada: entre a hipertecnologia e um conhecimento estético do cuidar

Introdução: Os sistemas de saúde estão a enfrentar uma realidade de radical intensidade biotecnológica, em que a digitalização ocupa um lugar central na prestação de cuidados. A inovação tecnológica e a cyborguização do humano devem traduzir-se em novos conceitos do cuidar, em que a ontologia cyborg tem um lugar central. Objectivos: Através de um enquadramento conceptual e tendo em conta os referentes teóricos da enfermagem sobre o cuidar, pretende-se estruturar uma análise epistemológica sobre a tecnologia, a ontologia cyborg e novos conceitos de "Cuidar em Enfermagem Metodologia: Revisão integrada de literatura, análise reflexiva e conceptual de quadros teóricos referenciais em enfermagem. Resultados: A investigação recente neste domínio baseia-se num pressuposto essencialista que remete para uma determinada conceção de "Tecnologia" enquanto aparato instrumental redutor, numa abordagem epistemológica positivista e mecanicista da tecnociência. As tecnologias de ponta aplicadas à enfermagem (onde se incluem as tecnologias de informação, plataformas digitais informáticas, sistemas de e-health, tele-enfermagem) são consideradas nesta perspetiva pragmática, como algo neutro, preexistente, uma mera ferramenta ou utensílio facilitador ao dispor dos enfermeiros. A análise dos discursos científicos assim construídos revela a adoção de uma visão instrumental da tecnologia. Afirma-se a clivagem entre pessoas e máquinas, a absoluta distinção entre tecnologia e a condição humana, num hiato entre objetos "técnicos" e objectos "naturais". Conclusões: A clivagem dicotómica entre "Tecnologia" e "Cuidado centrado na Pessoa" é artificial, fragmentária e contraria uma conceção integradora do cuidar. A dimensão estética no cuidar em enfermagem pressupõe a plena assunção da ontologia Cyborg. A conceção de uma "tecnologia encarnada" permite integrar na relação do cuidado uma hermenêutica do descentramento, incorporando, nos fenómenos alvo da intervenção de enfermagem, as paisagens relacionais, no sentido de relationscapes de Erin Manning (2013), onde os processos tecnológicos de saúde e doença e os cuidados são efetivamente vividos.

Um modelo de suporte à comunicação de agentes para tratamento de informação imprecisa baseada em sinônimos

Este trabalho propõe o desenvolvimento de um modelo de suporte á comunicação entre agentes e uma Ontologia com informações imprecisas. Os conceitos desta ontologia podem possuir sinônimos possibilitando a interpretação de termos linguísticos imprecisos. Tal problema é relacionado às questões de desenvolvimento da comunicação em Sistemas Multiagentes, possuindo como referência uma base de conhecimento da qual estes agentes possam requisitar informações. Neste estudo, a premissa do modelo é o de ser útil como componente na utilização por desenvolvedores que queiram utilizar de forma simplificada uma conexão com uma Ontologia para dar suporte na comunicação. Para o presente estudo são discutidos os conceitos sobre a comunicação no ambiente multiagentes. Também é realizada uma revisão sobre o desenvolvimento de Ontologias, de forma a criar uma ontologia para os agentes. A lógica nebulosa, baseado em variáveis linguísticas, servirá como modeladora da imprecisão da informação, dando suporte a essa questão com a Ontologia e a comunicação. De forma a validar o modelo proposto, é realizado um estudo de caso no sistema multiagentes das hortas urbanas do Parque San Jerónimo, de Sevilha, Espanha.

La inobservancia a las normas sociales: hendiduras a la ontología de la realidad social

El presente estudio ofrece una interpretación de naturaleza ontológica a uno de los fenómenos más divulgados recientemente por los medios informativos de comunicación: la displicencia de los sujetos al seguimiento de normas sociales. El trabajo se centra, especialmente, en los casos en los que se asume que la indiferencia a las normas tiene como causa la condición del privilegiado social. Argumenta que la inobservancia a las normas, más que ser un asunto de desigualdad social, es la sintomatología de importantes hendiduras en la ontología de la realidad social. Así, tres partes conforman este trabajo. En la primera se describen algunos casos que, desde los medios de comunicación, se presentan como representativos en la inobservancia a las normas sociales, especialmente, aquellos que son interpretados como un asunto de abuso de privilegio social. En la segunda parte, a partir de algunos trabajos de John Austin, Ulises Moulines y Gottlob Frege, se presenta una concepción de normas sociales, y su valor para la construcción de la ontología social. En la tercera parte, desde la propuesta del filósofo John Searle, se describen algunos rasgos esenciales de la ontología de la realidad social. Se concluye que el no seguimiento de las normas sociales socava los modos de existencia de la realidad social, y que los casos interpretados por los medios de comunicación como asunto de desigualdad social, requieren una interpretación que tenga en cuenta la estructura de la realidad social.

Imagens das emoções na infância: Representações sociais da alegria e da tristeza entre crianças em idade pré-escolar

As crianças são muito sensíveis a qualquer mudança no meio familiar ou social. O modo como apreendem, vivenciam e exprimem emoções ou sentimentos, em interacção com o meio - pais, professores e colegas determina, em muito, o seu desenvolvimento, nomeadamente a nível cognitivo, emocional e social. Nesta investigação averiguámos as representações sociais dos sentimentos, em particular da alegria e da tristeza, entre crianças com 5 e 6 anos, de ambos os sexos, que frequentam Jardins de Infância em Lisboa. Enquadrámos teoricamente a investigação na teoria das Representações Sociais (e.g. Moscovici,1976). Os dados foram recolhidos através de desenhos e por associação livre de palavras, a questões colocadas individualmente às crianças, e tratados através de AFO. Os resultados revelaram bastante acerca dos sentimentos das crianças, o que também nos estimula a reflectir sobre o modo mais cuidado para actuar com elas, contribuindo para o seu bem-estar psíquico e social, e aprendizagem. ABSTRACT; Children are deeply sensitive to any change in the family or social environment. The way in which they perceive, experience, and express emotions or feelings when interacting with the environment - parents, teachers, and fellow students - largely determines their development namely on a cognitive, emotional and social level. This research examines the social representations of feelings, particularly of joy and sadness, among children aged 5 and 6 years old, of the both sexes, and attending Kindergartens in Lisbon. On a theoretical perspective, the empirical research is framed on the Social Representations theory (e.g., Moscovici,1976). The data was gathered using drawings and free association, from questions individually made to the children, and examined through FCA2. The results showed quite a lot about the feelings of the children, which also leads us to reflect on the most careful way to act with them, as to contribute to their psychic and social well being, as well as their learning

CD81 interacting proteins

Fertilization is a multistep and complex process culminating in the merge of gamete membranes, cytoplasmic unity and fusion of genome. CD81 is a tetraspanin protein that participates in sperm-oocyte interaction, being present at the oocyte surface. CD81 has also been implicated in other biological processes, however its specific function and molecular mechanisms of action remain to be elucidated. The interaction between CD81 and its binding partner proteins may underlie the CD81 involvement in a variety of cellular processes and modulate CD81/interactors specific functions. Interestingly, in a Yeast two Hybrid system previously performed in our lab, CD81 has emerged as a putative interactor of the Amyloid Precursor Protein (APP). In the work here described, bioinformatics analyses of CD81 interacting proteins were performed and the retrieved information used to construct a protein-protein interaction network, as well as to perform Gene Ontology enrichment analyses. CD81 expression was further evaluated in CHO, GC-1 and SH-SY5Y cell lines, and in human sperm cells. Additionally, its subcellular localization was analyzed in sperm cells and in the neuronal-like SH-SY5Y cell line. Subsequently, coimmunoprecipitation assays were performed in CHO and SH-SY5Y cells to attempt to prove the physical interaction between CD81 and APP. A functional interaction between these two proteins was accessed thought the analyses of the effects of CD81 overexpression on APP levels. A co-localization analysis of CD81 and some interactors proteins retrieved from the bioinformatics analyses, such as APP, AKT1 and cytoskeleton-related proteins, was also performed in sperm cells and in SH-SY5Y cells. The effects of CD81 in cytoskeleton remodeling was evaluated in SH-SY5Y cells through monitoring the effects of CD81 overexpression in actin and tubulin levels, and analyzing the colocalization between overexpressed CD81 and F-actin. Our results showed that CD81 is expressed in all cell lines tested, and also provided the first evidence of the presence of CD81 in human sperm cells. CD81 immunoreactivity was predominantly detected in the sperm head, including the acrosome membrane, and in the midpiece, where it co-localized with APP, as well as in the post-acrosomal region. Furthermore, CD81 co-localizes with APP in the plasma membrane and in cellular projections in SH-SY5Y cells, where CD81 overexpression has an influence on APP levels, also visible in CHO cells. The analysis of CD81 interacting proteins such as AKT1 and cytoskeletonrelated proteins showed that CD81 is involved in a variety of pathways that may underlie cytoskeleton remodeling events, related to processes such as sperm motility, cell migration and neuritogenesis. These results deepen our understanding on the functions of CD81 and some of its interactors in sperm and neuronal cells.

Una aproximación para la descripción semántica de requisitos para categorización docentes de investigadores Ecuatorianos

La nueva generación de la Web, la Web Semántica, plantea potenciales oportunidades para dotar de significado a los contenidos Web. Las ontologías constituyen una de las principales herramientas para especificar explícitamente los conceptos de un dominio concreto, sus propiedades y sus relaciones; de manera que la información se publique en formatos que sean inteligibles automáticamente por agentes máquinas que pueden localizar y gestionar de forma precisa la información. En este trabajo se presenta un marco de trabajo para una red de ontologías para representar conceptos, atributos, operaciones y restricciones, en relación a los ítems curriculares que se usan en procesos nacionales de categorización de docentes universitarios ecuatorianos. En una primera parte se muestra el contexto del dominio, trabajos relacionados, luego se describe el proceso seguido, la abstracción del modelo ontológico y finalmente se presenta una ontología. Es una ontología de dominio debido a que proporciona el significado de los conceptos y sus relaciones dentro del dominio de ítems curriculares producidos por docentes universitarios, que son requisitos de los proceso de categorización docente universitaria en Ecuador.