Obtenção e caracterização de lipossomas unilamelares pequenos contendo cafeína

Due to great difficulty of penetration of drugs through skin, different organized systems, such as liposomes, have been studied in order to increase percutaneous penetration. The aims of this work were to obtain and characterize small unilamellar liposomes containing caffeine (CAF). Liposomes composed by soy phosphatidylcholine - PS (40 mM) or hydrogenated PS - PSH (40 mM), with and without cholesterol - CHO (6 mM) and CAF (30 mg/mL), were characterized by size distribution, determination of mean diameter and encapsulation efficiency. Uniform size distribution with low polidispersity was observed. The mean of diameters obtained were: PS/CHO (64 nm), PS (80 nm), PSH/CHO (85 nm), PS/CAF (145 nm), PS/CHO/CAF (147 nm), PSH/CHO/CAF (152 nm), PSH (166 nm) and PSH/CAF (481 nm). The obtained encapsulation efficiency was 10.84% for PSH/CHO/CAF, followed by PS/CHO (6.61%), PSH/CAF (3.07%) and PS/CAF (1.57%).

Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes

Numerous potentially mutagenic chemicals have been studied mainly because they can cause damaging and inheritable changes in the genetic material. Several tests are commonly used for biomonitoring pollution levels and to evaluate the effects of toxic and mutagenic agents present in the natural environment. This study aimed at assessing the potential of a textile effluent contaminated with azo dyes to induce chromosomal and nuclear aberrations in Allium cepa test systems. A continuous exposure of seeds in samples of the textile effluent in different concentrations was carried out (0.3%, 3%, 10%, and 100%). Cells in interphase and undergoing division were examined to assess the presence of chromosome aberrations, nuclear changes, and micronuclei. Our results revealed a mutagenic effect of the effluent at concentrations of 10% and 100%. At lower concentrations, the effluent (3% and 0.3%) did not induce mutagenic alterations in the test organism A. cepa. These findings are of concern, since cell damage may be transmitted to subsequent generations, possibly affecting the organism as a whole, as well as the local biota exposed to the effluent discharge. If the damage results in cell death, the development of the organism may be affected, which could also lead to its death. © 2008 Elsevier Ltd. All rights reserved.

Competencia bancaria en México

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Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm

In mammalian species, oocyte activation is initiated by oscillations in the intracellular concentration of free calcium ([Ca2+]i), which are also essential to allow embryonic development. To date, evidence supporting the hypothesis that a sperm factor is responsible for initiating oocyte activation has been presented in various mammalian species. Among the possible candidates to be the active sperm factor is the novel sperm-specific phospholipase C ζ (PLCζ), which besides its testis-specific expression is capable of initiating [Ca2+]i oscillations. In this study, we investigated the presence of PLCζ in the sperm of the domestic cat and whether normospermic and teratospermic cats differ in their PLCζ expression. Immunoblotting with anti-PLCζ antibodies confirmed the presence of an immunoreactive band of ~70 kDa in whole sperm lysates of domestic cat as well as in both soluble and insoluble fractions from this sperm. Additional immunoreactive bands, probably C- and N-terminal truncated versions of PLCζ, were also visualized in the soluble sperm fractions. Interestingly, immunoreactivity of PLCζ was detectable in teratospermic sperm, although with slightly less intensity than in normospermic sperm. In conclusion, domestic cat sperm express PLCζ in both cytosolic and high-pH fractions, which is consistent with data in other mammals. Sperm from teratospermic cats also express PLCζ, albeit at reduced concentrations, which may affect the fertility of these males. © 2013 Elsevier Inc..

Hacia la utopía: tenemos que ser menos desiguales

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Remodelação cardíaca induzida plea deficiência de vitamina D em ratos. -

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Istmo Centroamericano: estadísticas del subsector eléctrico: datos actualizados a 1998

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Polimorfismos no gene JY-1 e suas associações com probabilidade de prenhez precoce e características de crescimento em novilhas da raça Nelore

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interação genótipo-ambiente para a produção de leite em bubalinos no Brasil e na Colômbia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estructuras institucionales y mecanismos de promoción de exportaciones: las experiencias de México y Colombia

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Desenvolvimento e avaliação do desempenho de um equipamento para ensaio dinâmico de rodado agrícola individual

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Encontros vocálicos em português arcaico: uma interpretação fonológica

Este trabalho estuda os encontros de vogais na escrita do Português Arcaico (de agora em diante PA), no seu período trovadoresco, em busca de seus status fonológicos. Foram focalizados os encontros entre vogais no interior de palavras, como objetivo de definir se se está diante de ditongos ou hiatos. Utilizando a Fonologia Não-Linear, em especial, o modelo métrico, que trata da estrutura da sílaba, foi possível chegar a conclusões sobre a estruturação silábica do português da época e organizar hipóteses para interpretar os encontros vocálicos no nível fonológico. Finalmente, foi possível concluir que o tipo de encontro vocálico mais comum em PA, não por coincidência, é ditongo (foram constatados no corpus 722 ditongos e 123 hiatos), também o mais comum no Português Brasileiro atual. Mas é preciso ressaltar que o PA tolera os hiatos (inclusive não aceita outra solução, em alguns casos) enquanto o Português Brasileiro os evita. A conclusão a que se chega, a partir das análises empreendidas neste trabalho, é que, no PA, no nível fonológico, existem, no máximo, ditongos (os tritongos só são possíveis no nível fonético). E, mesmo no caso dos ditongos, o glide está posicionado na coda da sílaba (e não no núcleo).