A comparison of mucosal surface area and villous histology in small intestines of the brazilian free-tailed bat (Tadarida brasiliensis) and the mouse (Mus musculus)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

History and fate of a small isolated population of Weddell seals at White Island, Antarctica

Weddell seals (Leptonychotes weddellii Lesson) at White Island, Antarctica form a small, completely enclosed, natural population hypothesized to be of recent origin, likely founded by individuals from nearby Erebus Bay. This population constitutes an ideal model to document a founder event and ensuing genetic drift, with implications for conservation. Here we combined historical accounts, census and tagging data since the late 1960s, and genetic data (41 microsatellite loci and mitochondrial DNA sequences) from 84 individuals representing nearly all individuals present between 1990 and 2000 to investigate the history of the founding of the White Island population, document its population dynamics and evaluate possible future threats. We fully resolved parental relationships over three overlapping generations. Cytonuclear disequilibrium among the first generation suggested that it comprised the direct descendants of a founding group. We estimated that the White Island population was founded by a small group of individuals that accessed the island during a brief break in the surrounding sea ice in the mid-1950s, consistent with historical accounts. Direct and indirect methods of calculating effective population size were highly congruent and suggested a minimum founding group consisting of three females and two males. The White Island population showed altered reproductive dynamics compared to Erebus Bay, including highly skewed sex ratio, documented inbred mating events, and the oldest known reproducing Weddell seals. A comparison with the putative source population showed that the White Island population has an effective inbreeding coefficient (Fe) of 0.29. Based on a pedigree analysis including the hypothesized founding group, 86% of the individuals for whom parents were known had inbreeding coefficients ranging 0.09–0.31. This high level of inbreeding was correlated with reduced pup survival. Seals at White Island therefore face the combined effects of low genetic variability, lack of immigration, and inbreeding depression. Ultimately, this study provides evidence of the effects of natural isolation on a large, long-lived vertebrate and can provide clues to the potential effects of anthropogenic- caused isolation of similar taxa.

Karyotype characterization reveals an up and down of 45S and 5S rDNA sites in Crotalaria (Leguminosae-Papilionoideae) species of the section Hedriocarpae subsection Macrostachyae

The genus Crotalaria is one of the largest within the family Leguminosae-Papilionoideae, with more than 600 species. However, few karyotypes have been described. In the present paper, five species belonging to the section Hedriocarpae were studied (subsection Machrostachyae), in order to better understand chromosomal evolution in Crotalaria. The results reveals that all species presented 2n = 2x = 16 with symmetrical karyotypes, and slight differences in the chromosome morphology. A secondary constriction was identified at short arm of the pair 1. The 45S rDNA was mapped in the secondary constriction and adjacent heterochromatin (NOR-heterochromatin) and a minor site was identified in C. ochroleuca. The 5S rDNA was mapped linked to 45S rDNA at chromosome 1 short arm in all species. Additional sites for 5S rDNA were identified in C. pallida, C. striata and C. mucronata. Heterochromatin blocks around the centromeres are not CMA(+) neither DAPI(+). The karyotypes of the subsection Macrostachyae are characterized by an inversion at chromosome pair one in relation to previous specialized floral species analyzed. Additional sites of 45S and 5S rDNA were assumed to be a result of transposition events by different ways. The results suggest heterochromatin differentiation and the position of ribosomal genes indicates chromosomal rearrangements during evolution. Karyotype characteristics corroborate the morphological infrageneric classification.

Accuracy of Timed Up and Go Test for screening risk of falls among community-dwelling elderly

Objective: To determine the accuracy of the Timed Up and Go Test (TUGT) for screening the risk of falls among community-dwelling elderly individuals. Method: This is a prospective cohort study with a randomly by lots without reposition sample stratified by proportional partition in relation to gender involving 63 community-dwelling elderly individuals. Elderly individuals who reported having Parkinson's disease, a history of transitory ischemic attack, stroke and with a Mini Mental State Exam lower than the expected for the education level, were on a wheelchair and that reported a single fall in the previous six months were excluded. The TUGT, a mobility test, was the measure of interested and the occurrence of falls was the outcome. The performance of basic activities of daily living (ADL) and instrumental activities of daily living (IADL) was determined through the Older American Resources and Services, and the socio-demographic and clinical data were determined through the use of additional questionnaires. Receiver Operating Characteristic Curves were used to analyze the sensitivity and specificity of the TUGT. Results: Elderly individuals who fell had greater difficulties in ADL and IADL (p<0.01) and a slower performance on the TUGT (p=0.02). No differences were found in socio-demographic and clinical characteristics between fallers and non- fallers. Considering the different sensitivity and specificity, the best predictive value for discriminating elderly individuals who fell was 12.47 seconds [(RR= 3.2) 95% CI: 1.3- 7.7]. Conclusions: The TUGT proved to be an accurate measure for screening the risk of falls among elderly individuals. Although different from that reported in the international literature, the 12.47 second cutoff point seems to be a better predictive value for Brazilian elderly individuals.

Factors Associated with Height Catch-Up and Catch-Down Growth Among Schoolchildren

In developed countries, children with intrauterine growth restriction (IUGR) or born preterm (PT) tend to achieve catch-up growth. There is little information about height catch-up in developing countries and about height catch-down in both developed and developing countries. We studied the effect of IUGR and PT birth on height catch-up and catch-down growth of children from two cohorts of liveborn singletons. Data from 1,463 children was collected at birth and at school age in Ribeirao Preto (RP), a more developed city, and in Sao Luis (SL), a less developed city. A change in z-score between schoolchild height z-score and birth length z-score >= 0.67 was considered catch-up; a change in z-score <=-0.67 indicated catch-down growth. The explanatory variables were: appropriate weight for gestational age/PT birth in four categories: term children without IUGR (normal), IUGR only (term with IUGR), PT only ( preterm without IUGR) and preterm with IUGR; infant's sex; maternal parity, age, schooling and marital status; occupation of family head; family income and neonatal ponderal index (PI). The risk ratio for catch-up and catch-down was estimated by multinomial logistic regression for each city. In RP, preterms without IUGR (RR = 4.13) and thin children (PI<10th percentile, RR = 14.39) had a higher risk of catch-down; catch-up was higher among terms with IUGR (RR = 5.53), preterms with IUGR (RR = 5.36) and children born to primiparous mothers (RR = 1.83). In SL, catch-down was higher among preterms without IUGR (RR = 5.19), girls (RR = 1.52) and children from low-income families ( RR = 2.74); the lowest risk of catch-down (RR = 0.27) and the highest risk of catch-up (RR = 3.77) were observed among terms with IUGR. In both cities, terms with IUGR presented height catch-up growth whereas preterms with IUGR only had height catch-up growth in the more affluent setting. Preterms without IUGR presented height catch-down growth, suggesting that a better socioeconomic situation facilitates height catch-up and prevents height catch-down growth.

Lumpiness, capital adjustment costs and investment dynamics

Aggregate investment in the US economy displays a hump-shaped pattern in response to shocks, and the autocorrelation of aggregate investment growth is positive for the first few quarters, turning negative for the later quarters. This paper shows that this feature of the data is the natural outcome of a two-sector consumption/investment model designed and calibrated to reproduce plant-level evidence on capita: accumulation. (C) 2012 Elsevier B.V. All rights reserved.

Elf magnetic field influence on ION Channels studied by Patch Clamp Technique: exposure set up and "Whole Cell" measurements on Potassium currents

The aim of this thesis was to study the effects of extremely low frequency (ELF) electromagnetic magnetic fields on potassium currents in neural cell lines ( Neuroblastoma SK-N-BE ), using the whole-cell Patch Clamp technique. Such technique is a sophisticated tool capable to investigate the electrophysiological activity at a single cell, and even at single channel level. The total potassium ion currents through the cell membrane was measured while exposing the cells to a combination of static (DC) and alternate (AC) magnetic fields according to the prediction of the so-called ‘ Ion Resonance Hypothesis ’. For this purpose we have designed and fabricated a magnetic field exposure system reaching a good compromise between magnetic field homogeneity and accessibility to the biological sample under the microscope. The magnetic field exposure system consists of three large orthogonal pairs of square coils surrounding the patch clamp set up and connected to the signal generation unit, able to generate different combinations of static and/or alternate magnetic fields. Such system was characterized in term of field distribution and uniformity through computation and direct field measurements. No statistically significant changes in the potassium ion currents through cell membrane were reveled when the cells were exposed to AC/DC magnetic field combination according to the afore mentioned ‘Ion Resonance Hypothesis’.

Beyond socialism: Tanzanian theatre, neoliberalism and foreign aid complexity

Theatralität ist ein gängiges Konzept, um Theater in Afrika zu definieren. Wird dieses Konzept angewendet, so treten die historischen Unterschiede zwischen den verschiedenen Theaterformen in den Hintergrund. Deshalb ist es wichtig, Theater in einen kulturellen Kontext zu stellen, aus dem das Theater entsteht. Dadurch können nationale und internationale Suprastrukturen, die die sozialpolitische und wirtschaftliche Atmosphäre bestimmen, analysiert werden,. Da sich die aktuelle „globale“ Entwicklung auf neoliberale Grundsätze stützt, ist es offensichtlich, dass man Theater nicht diskutieren kann, ohne näher auf Neoliberalismus, Imperialismus, Kapitalismus, Entwicklungshilfe und Geberpolitik einzugehen.rnDerzeit werden die meisten Theaterprojekte in Tansania durch die Entwicklungshilfe oder ausländische Geberorganisationen unterstützt. Diese Organisationen stellen finanzielle Mittel zur Verfügung, um Theaterproduktionen auf unterschiedlichem Niveau zu ermöglichen. Diese Spendenpraxis hat zu der Fehlannahme geführt, dass Theater nur dann ein Theater ist, wenn es durch ausländische Organisationen finanziert wird. Jedoch ist es offensichtlich, dass diese finanziellen Mittel eine große Rolle in der Machtpolitik spielen. Diese Studie untersucht deshalb die Frage: Welchen Einfluss hat die neoliberale Politik, insbesondere durch die Entwicklungshilfe, auf das Theater in Tansania? Die Arbeit deckt einmal die Verbindung zwischen dem produzieren Theater und den verschiedenen dominierenden politischen Richtungen – von Nationalismus bis Neoliberalismus – auf. Darüber hinaus wird gezeigt, dass diese Verbindungen es dem Theater erschweren, diese Suprastrukturen zu vermeiden, durch die es finanziert wird. Das bedeutet, dass die neoliberale Politik mit seinen Merkmalen von Einengung, Unterdrückung und Ausbeutung auch ein eingeengtes, unterdrücktes und ausbeuterisches Theater hervorbringt. Dieser Studie bezeichnet ein solches Theater als Theater (Neo-)Liberalismus. Es ist ein Theater, das apolitisch auftritt, aber tatsächlich unter der neoliberal Politik des freien Markts und der Subventionsstreichungen ums Überleben kämpft.rnIndem diese Verbindungen zwischen Theater, Entwicklungshilfe und Geberorganisationen erläutert werden, kommt diese Forschung zu folgendem Ergebnis: Die Geberorganisationen haben kein Recht, unabhängig von der Höhe ihrer Spende, in die Souveränität eines Staates einzugreifen oder ein neues System einzuführen. Deshalb sollte die Loslösung von ausländischen Geberländern an erster Stelle stehen, damit sich das Theater ganz entwickeln und unabhängig überleben kann. Es ist deshalb notwendig, das Konzept des Volkstheaters neu zu definieren. Das Theater soll wieder mit den Initiativen von Menschen zu tun haben und ihre eigenen Themen in einem gewissen zeitlich und räumlichen Rahmen ansprechen.rnrn

Misure di audience nell'Internet Advertising: studio di caso della start-up "Taggalo"

L’avvento di Internet e delle innovazioni tecnologiche hanno determinato una rivoluzione nel panorama della comunicazione e dell’informazione soprattutto in quello pubblicitario. La pubblicità online (in inglese Advertising Online o Internet Advertising) è un fenomeno in continua crescita. Rispetto ai media tradizionali come la stampa e la televisione dove i contenuti sono trasmessi in modo generico e di massa, l’Advertising Online sfrutta la capacità del World Wide Web al fine di raggiungere una quantità notevole di persone permettendo di attuare campagne pubblicitarie verso un target mirato. In questo settore la facilità di misurare l'effetto nei confronti del pubblico permette alle aziende di investire in questo tipo di mercato rispetto a quello tradizionale poiché, in quest'ultimo l’assenza di strumenti di misurazione è basata su rilevanze teoriche. In riferimento a quanto citato, un’oggetto di studio e di analisi presentato in questa tesi riguarda la start-up TAGGALO un sistema ideato e sviluppato da un gruppo di ricercatori del CNR Puglia. Questa soluzione tecnologia è applicata nel campo pubblicitario del Digital out of Home e del Digital Signage dove la comunicazione del messaggio viene percepita dal potenziale cliente mentre è fuori casa mediante l’uso di schermi elettronici disposti nella maggior parte dei luoghi pubblici. Attraverso questa tecnologia è possibile rilevare il reale impatto della pubblicità online, rappresentando una soluzione efficace per misurare il reale ritorno per chi investe ovvero il ROI.

Analisi start-up settore digitale. Il ruolo del management.

Questo lavoro di ricerca studia il mercato digitale delle Start-Up italiane, valutando i passi del management nei vari cicli di vita aziendale. Dopo uno studio teorico sull'ecosistema italiano startup e il cambiamento dell'approccio scelto dal manager all'interno dell'azienda per arrivare al successo, è stato fatto un studio empirico con questionari e interviste. L'intento è capire in un team aziendale quali sono le figure da seguire e con che importanza. Quanto è fondamentale la figura di colui che organizza, media e detiene la responsabilità del lavoro finito nei tempi prestabiliti?

Experimental set up and characterization of a prototype for breast microwave imaging

Nella presente tesi è stato sviluppato un sistema di acquisizione automatico finalizzato allo studio del breast microwave imaging. Le misure sono state eseguite in configurazione monostatica, in cui viene acquisito un segnale da diverse posizioni lungo il perimetro dell’area di indagine. A questo scopo, è stato installato un motore ad alta precisione che permette la rotazione del fantoccio e l’esecuzione automatica delle misure da un numero di posizioni fissato. Per automatizzare il processo di acquisizione, è stato inoltre sviluppato appositamente un software in ambiente LabView. Successivamente, è stata eseguita una intensa sessione di misure finalizzate alla caratterizzazione del sistema sviluppato al variare delle condizioni di misura. Abbiamo quindi utilizzato dei fantocci di tumore di diverse dimensioni e permittività elettrica per studiare la sensibilità della strumentazione in condizione di mezzo omogeneo. Dall’analisi delle ricostruzioni multifrequenza effettuate tramite diversi algoritmi di tipo TR-MUSIC sul range di frequenze selezionato, abbiamo notato che il tumore è ricostruito correttamente in tutti gli scenari testati. Inoltre, abbiamo creato un ulteriore fantoccio per simulare la presenza di una disomogeneità nel dominio di imaging. In questo caso, abbiamo studiato le performances del sistema di acquisizione al variare della posizione del tumore, le cui caratteristiche sono state fissate, e della permittività associata al fantoccio. Dall’analisi dei risultati appare che le performances di ricostruzione sono condizionate dalla presenza della disomogeneità, in modo particolare se il tumore è posizionato all’interno di essa. Infine, abbiamo studiato delle performance di due algoritmi di ricostruzione 3D: uno di essi è basato sulla sovrappo- sizione tomografica e sfrutta metodi di interpolazione, l’altro si basa sull’utilizzo di un propagatore 3D per il dipolo Hertziano in approssimazione scalare.