Kinetic and mechanistic studies on unconventional antioxidant activities of natural compounds

In chapter one, the autoxidation kinetics of natural oil substrates, including, triglyceric sunflower oil, olive oil, terpenic squalene, and p-cymene were calibrated through differential oximetry methods. Calibration allows their use as reference oxidizable substrates for further studies, e.g. for quantitative testing of antioxidants under biomimetic settings. Several essential oils samples, of different botanical species or different productions of same species were studied for their antioxidant activity in inhibited autoxidation kinetics. Their antioxidant activities were matched with their composition analyzed by GC-MS. In chapter two, the molecular mechanism of the synergy between the common phenolic antioxidants such as tocopherol and catechols with widespread essential component gamma-terpinene was studied through lipid oxidation kinetics. Wherein, gamma-terpinene was able to disclose the key intermediacy HOO·, which acted as a reducing agent regenerating the phenolic antioxidant. This counterintuitive role of HOO· radicals was further investigated in detail and allowed to rationalize for the first time the purported antioxidant behavior of PDA melanin nanoparticles. It will also open to a deeper understanding of the redox biology of quinones. Regarding melanin, its role is broadly important in living organisms and its control, including its inhibition, is of great importance with several relevant applications ranging from food preservation to control of human skin pigmentation. In chapter three, an oximetry method combined with the traditional UV-Vis spectroscopy was developed to study the tyrosinase inhibition kinetics, which allowed identifying Glabridin (from G. glabra, L.), as one of the most effective natural tyrosinase inhibitors.

Neuro-ophthalmological and electrophysiological characterization of mitochondrial diseases.

Aims and methods: 1) characterization of patients with Dominant Optic Atrophy (DOA) associated with mutations in AFG3L2 and ACO2 genes in comparison with classical OPA1-DOA; 2) characterization of patients with mtDNA mutations causing MELAS and MERRF syndromes and correlation with heteroplasmy; 3) longitudinal evaluation of subacute m.11778G>A/MTND4 Leber’s Hereditary Optic Neuropathy (LHON) patients co-treated with rAAV2/2-ND4 gene therapy and idebenone. We performed a comprehensive neuro-ophthalmological assessment coupled with electrophysiological examination. Results: 1) We described and compared 23 ACO2 and 13 AFG3L2 patients with 72 OPA1 patients. All patients presented temporally predominant optic atrophy, with ACO2 showing higher RNFL and GCL thicknesses at OCT, while AFG3L2 was virtually-indistinguishable from OPA1. 2) Retinopathy was the most common manifestation in 17/33 MELAS patients, conversely, optic atrophy was the most common finding in 7/8 MERRF patients. Correlation of heteroplasmy with neuro-ophthalmological parameters failed to disclose any significance in MELAS, while it negatively correlated with OCT parameters in MERRF. 3) We compared modifications in visual acuity, OCT and electrophysiological parameters at 3 timepoints in 9 LHON patients. We observed significant decrease of RNFL thickness and reduction of PhNR amplitude. Visual acuity improved of about -0.37 LogMAR, correlating significantly with time from onset and from injection, but not with idebenone therapy duration. Discussion: 1) ACO2 seems associated to better preservation of retinal ganglion cells, depending on a different pathogenic mechanism involving mtDNA maintenance, as opposed to AFG3L2 which is involved in OPA1 processing. 2) MELAS and MERRF patients presented with a clearly distinct ocular phenotype, possibly reflecting a selective susceptibility of different retinal cell types to global energy defect or oxidative stress. 3) Follow up of LHON patients treated with gene therapy confirmed the deterioration in OCT and electrophysiological parameters, while the amount of visual improvement was similar to the one observed in recent clinical trials.

Email Evidence Preservation. How to Balance the Obligation and the High Cost

With the great advancement of computer technologies, electronic information starts to play a more and more important role in modern business transactions. Therefore, electronic data, such as e-mail, is frequently required in the process of litigation. Companies, on the one hand, have the legal obligations to produce this kind of e-mail evidence. On the other hand, they also undertake a high cost of e-mail evidence preservation due to the great volume on a daily basis. This Article firstly analyzed features of e-mail evidence with the comparison of paper evidence. Then, it discussed about how e-mail is authenticated and admitted into evidence. By using the case laws in different legal aspects and current Canadian legislations, the Author demonstrated the importance of e-mail evidence preservation in ordinary business course. After that, the Article focused on the practical dilemma of the companies between their legal obligation and the expensive cost to preserve e-mail evidence. Finally, the Author proposed suggestions to both companies and courts on how to coordinate the obligation and cost. More specifically, while companies should adopt a document management policy to implement e-mail evidence preservation, courts need to take into consideration of the high cost of e-mail evidence preservation in electronic discovery.

“The Ministry of Obligation warns”: it is necessary to protect the sexual offender adolescents

This text deals with a part of the research-action “Multifamiliar groups with sexual offender adolescents” and emphasizes the written production of the adolescents during the process. two texts were involved: the first one was a letter addressed to the parents and the second was an evaluation of the multifamiliar group. Seven adolescents between 14 and 17 years old wrote the texts. About the first text we discussed: the adolescent as responsible for the domestic work; the adolescent and his need to receive support and protection and the adolescent and the recognition of his growth phase. about the second text: the feelings of the adolescent regarding sexual abuse; the relation with the institution that carries out the intervention; the symbols that identify his ambivalence. We comment about the sexual abuse practiced by these adolescents as from two main points: the adolescence seen as a development phase and the role played by the family in the conduction of this phase.

A dissertation on the right and obligation of the civil magistrate to take care of the interest of religion, and provide for its support; in which the arguments in confirmation of said right and obligation, both from reason and the Sacred Scriptures, are adduced: the usual objections examined,--together with several corollaries deduced from the subject.

to Act of Congress.>

An appeal to dissenters, on their submitting to the obligation imposed by law, for the religious celebration of marriage, according to the form prescribed in the Book of Common Prayer.

Mode of access: Internet.

United States export policy toward Iraq prior to Iraq's invasion of Kuwait : hearing before the Committee on Banking, Housing, and Urban Affairs, United States Senate, One Hundred Second Congress, second session, on did U.S. exports aid Iraq's military capabilities and did the administration accurately disclose its licensing of dual use exports to Iraq? : October 27, 1992.

Shipping list no.: 93-0202-P.

An essay on moral obligation : with a view towards settling the controversy, concerning moral and positive duties in answer to two late pamphlets, the one entitled, The true foundation of natural and revealed religion asserted being a reply to the supplement to the treatise on the Christian sacraments, the other [long dash] Some reflections upon the comparative excellency and usefulness of moral and positive duties by Mr. Chubb.

Signatures: 1 leaf unsigned, B-I⁴, K-L⁴, M².

An appeal to the candid of all denominations : in which the obligation, subjects, and mode of baptism are discussed / by Henry Slicer.

Mode of access: Internet.

God's message to the young : or, The obligation and the advantages of early piety, seriously urged upon young persons, in connexion with Eccles. xii.1. /

Mode of access: Internet.

Letter and accompanying documents from John W. Garrett, president of the Baltimore and Ohio Railroad Company, to the comptroller of Maryland, in relation to the obligation of the state, to provide for remitting to England the interest due on certain sterling bonds of the state ...

Mode of access: Internet.